Using psychological and physiological measures in arts-based activities in a community sample of people with a dementia and their caregivers: a feasibility and pilot study

Introduction: Nearly all community-based dementia care studies employ either qualitative methods or use a combination of self-report questionnaire within mixed-methods research designs. Physiological measures, however, are rarely used in community-based studies with this population yet could provide valuable biological information for specific activities across the course of the dementias. Method: The study employed a within-subjects design to assess the feasibility of obtaining physiological measures (salivary cortisol and Heart Rate Variability (HRV)) alongside subjective measures of wellbeing and stress using Visual Analogue Scales (VAS) during two community-based activiti of choral singing and art viewing, respectively, for People With Dementia (PWD) in the early to middle stages of impairment and their caregivers. Results: As anticipated, pre-post subjective wellbeing measures were relatively easy to complete by PWD and caregivers with no substantive difficulties observed or reported; significant increases in composite wellbeing, happiness and optimism were found. Continuous measurement of physiological data of HRV through a sensor-based device (Empatica® E4) was also found to be neither non-intrusive to participants nor disruptive to the interventions. Preliminary indications showed, for example, HRV significantly increased for PWD during choral singing, which may be associated with an increase in wellbeing. Measuring stress hormones through pre-post saliva samples, however, encountered several difficulties. Conclusions: The findings provide support for the feasibility of using non-intrusive sensor-based physiological measures alongside subjective measures for this population. Subjective measures may also give more confidence in interpreting directionality of physiological measures. A full pilot study is warranted to further investigate interactions of physiological and psychological variables in choral singing and viewing art activities but it is uncertain whether measuring stress hormones through saliva collection is feasible for this population and raised doubts about their use in a large scale trial. Holding particular promise is the use of sensor-based technology across different stages of dementia as well as across different activities

Differing calcification processes in cultured vascular smooth muscle cells and osteoblasts

© 2019 Published by Elsevier Inc.Arterial medial calcification (AMC) is the deposition of calcium phosphate mineral, often as hydroxyapatite, inthe medial layer of the arteries. AMC shares some similarities to skeletal mineralisation and has been associatedwith the transdifferentiation of vascular smooth muscle cells (VSMCs) towards an osteoblast-like phenotype. Thisstudy used primary mouse VSMCs and calvarial osteoblasts to directly compare the established and widely usedin vitromodels of AMC and bone formation. Significant differences were identified between osteoblasts andcalcifying VSMCs. First, osteoblasts formed large mineralised bone nodules that were associated with widespreaddeposition of an extracellular collagenous matrix. In contrast, VSMCs formed small discrete regions of calcifi-cation that were not associated with collagen deposition and did not resemble bone. Second, calcifying VSMCsdisplayed a progressive reduction in cell viability over time (≤7-fold), with a 50% increase in apoptosis,whereas osteoblast and control VSMCs viability remained unchanged. Third, osteoblasts expressed high levels ofalkaline phosphatase (TNAP) activity and TNAP inhibition reduced bone formation by to 90%. TNAP activity incalcifying VSMCs was∼100-fold lower than that of bone-forming osteoblasts and cultures treated withβ-gly-cerophosphate, a TNAP substrate, did not calcify. Furthermore, TNAP inhibition had no effect on VSMC calci-fication. Although, VSMC calcification was associated with increased mRNA expression of osteoblast-relatedgenes (e.g. Runx2, osterix, osteocalcin, osteopontin), the relative expression of these genes was up to 40-foldlower in calcifying VSMCs versus bone-forming osteoblasts. In summary, calcifying VSMCsin vitrodisplay somelimited osteoblast-like characteristics but also differ in several key respects: 1) their inability to form collagen-containing bone; 2) their lack of reliance on TNAP to promote mineral deposition; and, 3) the deleterious effectof calcification on their viability.Peer reviewedFinal Published versio

Plus One: HIV diagnosis and disclosure

Plus One: HIV sero-discordant relationships among black African people in England (NAHIP) Duration: September 2010 - November 2011 Plus One involved in-depth, face-to-face interviews with black African people living in England who were in relationships where one person had diagnosed HIV and the other did not (ie. HIV serodiscordant)

Dust attenuation in 2<z<3 star-forming galaxies from deep ALMA observations of the Hubble Ultra Deep Field

17 pages, 7 figures, accepted version to be published in MNRASWe present the results of a new study of the relationship between infrared excess (IRX ≡ L IR/L UV), ultraviolet (UV) spectral slope (β) and stellar mass at redshifts 2 < z < 3, based on a deep Atacama Large Millimeter Array (ALMA) 1.3-mm continuum mosaic of the Hubble Ultra Deep Field. Excluding the most heavily obscured sources, we use a stacking analysis to show that z ≃ 2.5 star-forming galaxies in the mass range 9.25 ≤ log(M*/M ⊙) ≤ 10.75 are fully consistent with the IRX-β relation expected for a relatively grey attenuation curve, similar to the commonly adopted Calzetti law. Based on a large, mass-complete sample of 2 ≤ z ≤ 3 star-forming galaxies drawn frommultiple surveys, we proceed to derive a new empirical relationship between β and stellar mass, making it possible to predict UV attenuation (A1600) and IRX as a function of stellar mass, for any assumed attenuation law. Once again, we find that z ≃ 2.5 star-forming galaxies follow A1600-M* and IRX-M* relations consistent with a relatively grey attenuation law, and find no compelling evidence that star-forming galaxies at this epoch follow a reddening law as steep as the Small Magellanic Cloud (SMC) extinction curve. In fact, we use a simple simulation to demonstrate that previous determinations of the IRX-β relation may have been biased towards low values of IRX at red values of β, mimicking the signature expected for an SMC-like dust law. We show that this provides a plausible mechanism for reconciling apparently contradictory results in the literature and that, based on typical measurement uncertainties, stellar mass provides a cleaner prediction of UV attenuation than β. Although the situation at lower stellar masses remains uncertain, we conclude that for 2 < z < 3 star-forming galaxies with log(M*/M ⊙) ≥ 9.75, both the IRX-β and IRX-M* relations are well described by a Calzetti-like attenuation law.Peer reviewe

Plus One: Managing the relationship

Plus One: HIV sero-discordant relationships among black African people in England (NAHIP) Duration: September 2010 - November 2011 Plus One involved in-depth, face-to-face interviews with black African people living in England who were in relationships where one person had diagnosed HIV and the other did not (ie. HIV serodiscordant)

Plus One: External Influences

Plus One: HIV sero-discordant relationships among black African people in England (NAHIP) Duration: September 2010 - November 2011 Plus One involved in-depth, face-to-face interviews with black African people living in England who were in relationships where one person had diagnosed HIV and the other did not (ie. HIV serodiscordant). Catherine Dodds, Peter , Annabel , Edith , John , Lawrence , Pamela , Kathie & Gary

Plus One: Sex and risk

Plus One: HIV sero-discordant relationships among black African people in England (NAHIP) Duration: September 2010 - November 2011 Plus One involved in-depth, face-to-face interviews with black African people living in England who were in relationships where one person had diagnosed HIV and the other did not (ie. HIV serodiscordant)

ISUOG Safety Committee updated recommendation on use of respirators by practitioners undertaking obstetric and gynecological ultrasound in context of SARS-CoV-2 Omicron variant of concern

status: publishe

Plus One: Executive Summary

Plus One: HIV sero-discordant relationships among black African people in England (NAHIP) Duration: September 2010 - November 2011 Plus One involved in-depth, face-to-face interviews with black African people living in England who were in relationships where one person had diagnosed HIV and the other did not (ie. HIV serodiscordant)