Pathogénicité résiduelle d'une souche de virus de la stomatite vésiculeuse contagieuse (Indiana) de culture cellulaire III. — Histopathologie des lésions cutanéomuqueuses et cytologie cellulaire

Joubert L., Tuaillon P., Prave Michel, Chabrouty Françoise, Fédida Maurice, Desmettre Ph. Pathogénicité résiduelle d’une souche de virus de la stomatite vésiculeuse contagieuse (Indiana) de culture cellulaire. III. Histopathologie des lésions cutanéomuqueuses et cytologie cellulaire. In: Bulletin de l'Académie Vétérinaire de France tome 126 n°3, 1973. pp. 145-154

Self-healing capability of concrete with crystalline admixtures in different environments

The aim of this study is analyzing the self-healing effect of a crystalline admixture in four types of environmental exposure comparing with a reference concrete. Healing was studied by means of permeability tests on cracked specimens and physical closing of the crack was observed by optic microscope and quantified through crack geometrical parameters. The studied crack openings were under 300 pm and the time set for healing was 42 days. The results show a different healing behavior depending on the exposure and the presence of the crystalline admixture, demonstrating that the presence of water is necessary for the healing reactions. (C) 2015 Elsevier Ltd. All rights reserved.Roig Flores, M.; Moscato, S.; Serna Ros, P.; Ferrara, L. (2015). Self-healing capability of concrete with crystalline admixtures in different environments. Construction and Building Materials. 86:1-11. doi:10.1016/j.conbuildmat.2015.03.091S1118

Pre-hospital management protocols and perceived difficulty in diagnosing acute heart failure

Aim To illustrate the pre-hospital management arsenals and protocols in different EMS units, and to estimate the perceived difficulty of diagnosing suspected acute heart failure (AHF) compared with other common pre-hospital conditions. Methods and results A multinational survey included 104 emergency medical service (EMS) regions from 18 countries. Diagnostic and therapeutic arsenals related to AHF management were reported for each type of EMS unit. The prevalence and contents of management protocols for common medical conditions treated pre-hospitally was collected. The perceived difficulty of diagnosing AHF and other medical conditions by emergency medical dispatchers and EMS personnel was interrogated. Ultrasound devices and point-of-care testing were available in advanced life support and helicopter EMS units in fewer than 25% of EMS regions. AHF protocols were present in 80.8% of regions. Protocols for ST-elevation myocardial infarction, chest pain, and dyspnoea were present in 95.2, 80.8, and 76.0% of EMS regions, respectively. Protocolized diagnostic actions for AHF management included 12-lead electrocardiogram (92.1% of regions), ultrasound examination (16.0%), and point-of-care testings for troponin and BNP (6.0 and 3.5%). Therapeutic actions included supplementary oxygen (93.2%), non-invasive ventilation (80.7%), intravenous furosemide, opiates, nitroglycerine (69.0, 68.6, and 57.0%), and intubation 71.5%. Diagnosing suspected AHF was considered easy to moderate by EMS personnel and moderate to difficult by emergency medical dispatchers (without significant differences between de novo and decompensated heart failure). In both settings, diagnosis of suspected AHF was considered easier than pulmonary embolism and more difficult than ST-elevation myocardial infarction, asthma, and stroke. Conclusions The prevalence of AHF protocols is rather high but the contents seem to vary. Difficulty of diagnosing suspected AHF seems to be moderate compared with other pre-hospital conditions

Intradermal Indocyanine Green for In Vivo Fluorescence Laser Scanning Microscopy of Human Skin: A Pilot Study

BACKGROUND: In clinical diagnostics, as well as in routine dermatology, the increased need for non-invasive diagnosis is currently satisfied by reflectance laser scanning microscopy. However, this technique has some limitations as it relies solely on differences in the reflection properties of epidermal and dermal structures. To date, the superior method of fluorescence laser scanning microscopy is not generally applied in dermatology and predominantly restricted to fluorescein as fluorescent tracer, which has a number of limitations. Therefore, we searched for an alternative fluorophore matching a novel skin imaging device to advance this promising diagnostic approach. METHODOLOGY/PRINCIPAL FINDINGS: Using a Vivascope®-1500 Multilaser microscope, we found that the fluorophore Indocyanine-Green (ICG) is well suited as a fluorescent marker for skin imaging in vivo after intradermal injection. ICG is one of few fluorescent dyes approved for use in humans. Its fluorescence properties are compatible with the application of a near-infrared laser, which penetrates deeper into the tissue than the standard 488 nm laser for fluorescein. ICG-fluorescence turned out to be much more stable than fluorescein in vivo, persisting for more than 48 hours without significant photobleaching whereas fluorescein fades within 2 hours. The well-defined intercellular staining pattern of ICG allows automated cell-recognition algorithms, which we accomplished with the free software CellProfiler, providing the possibility of quantitative high-content imaging. Furthermore, we demonstrate the superiority of ICG-based fluorescence microscopy for selected skin pathologies, including dermal nevi, irritant contact dermatitis and necrotic skin. CONCLUSIONS/SIGNIFICANCE: Our results introduce a novel in vivo skin imaging technique using ICG, which delivers a stable intercellular fluorescence signal ideal for morphological assessment down to sub-cellular detail. The application of ICG in combination with the near infrared laser opens new ways for minimal-invasive diagnosis and monitoring of skin disorders

Permanent 125I-seed prostate brachytherapy: early prostate specific antigen value as a predictor of PSA bounce occurrence

<p>Abstract</p> <p>Purpose</p> <p>To evaluate predictive factors for PSA bounce after ¹²⁵I permanent seed prostate brachytherapy and identify criteria that distinguish between benign bounces and biochemical relapses.</p> <p>Materials and methods</p> <p>Men treated with exclusive permanent ¹²⁵I seed brachytherapy from November 1999, with at least a 36 months follow-up were included. Bounce was defined as an increase ≥ 0.2 ng/ml above the nadir, followed by a spontaneous return to the nadir. Biochemical failure (BF) was defined using the criteria of the Phoenix conference: nadir +2 ng/ml.</p> <p>Results</p> <p>198 men were included. After a median follow-up of 63.9 months, 21 patients experienced a BF, and 35.9% had at least one bounce which occurred after a median period of 17 months after implantation (4-50). Bounce amplitude was 0.6 ng/ml (0.2-5.1), and duration was 13.6 months (4.0-44.9). In 12.5%, bounce magnitude exceeded the threshold defining BF. Age at the time of treatment and high PSA level assessed at 6 weeks were significantly correlated with bounce but not with BF. Bounce patients had a higher BF free survival than the others (100% versus 92%, p = 0,007). In case of PSA increase, PSA doubling time and velocity were not significantly different between bounce and BF patients. Bounces occurred significantly earlier than relapses and than nadir + 0.2 ng/ml in BF patients (17 vs 27.8 months, p < 0.0001).</p> <p>Conclusion</p> <p>High PSA value assessed 6 weeks after brachytherapy and young age were significantly associated to a higher risk of bounces but not to BF. Long delays between brachytherapy and PSA increase are more indicative of BF.</p

Evaluation of parenteral vaccination methods with glycoproteins against Aujeszky's disease in pigs

A comparative evaluation of vaccination methods with glycoproteins for the induction of immune responses and protection of the pig against Aujeszky's disease virus (ADV) was performed. Different vaccination routes (intradermal (I.D.) versus intramuscular (I.M.)), inoculation sites (the neck versus the back) and number of inoculation points (2 versus 6) per site were compared, Body weight (BW) changes and viral excretion after challenge were compared with virus-neutralizing titers, antigen-specific IgG and IgA responses in serum and virus-specific lymphoproliferative responses in peripheral blood during the immunisation period. According to BW changes better protection was obtained with six-point than two-point I.D. injections. I.D. vaccination in the back at six points gave similar results as I.M. vaccination in the neck but appeared inferior in the reduction of virus excretion, Regarding the immunological parameters, the virus-specific IgA response in serum gave the best indication for protection. It can be concluded that according to BW changes, six-point I.D. immunisation in the back and I.M. immunisation in the neck provided the best protection and that six-point I.D. injections resulted in a better vaccination than two-point I.D. injections. (C) 1997 Elsevier Science B.V.status: publishe