Preliminary design of a fuel cell/battery hybrid powertrain for a heavy-duty yard truck for port logistics

Abstract The maritime transport and the port-logistic industry are key drivers of economic growth, although, they represent major contributors to climate change. In particular, maritime port facilities are typically located near cities or residential areas, thus having a significant direct environmental impact, in terms of air and water quality, as well as noise. The majority of the pollutant emissions in ports comes from cargo ships, and from all the related ports activities carried out by road vehicles. Therefore, a progressive reduction of the use of fossil fuels as a primary energy source for these vehicles and the promotion of cleaner powertrain alternatives is in order. The present study deals with the design of a new propulsion system for a heavy-duty vehicle for port applications. Specifically, this work aims at laying the foundations for the development of a benchmark industrial cargo–handling hydrogen-fueled vehicle to be used in real port operations. To this purpose, an on-field measurement campaign has been conducted to analyze the duty cycle of a commercial Diesel-engine yard truck currently used for terminal ports operations. The vehicle dynamics has been numerically modeled and validated against the acquired data, and the energy and power requirements for a plug-in fuel cell/battery hybrid powertrain replacing the Diesel powertrain on the same vehicle have been evaluated. Finally, a preliminary design of the new powertrain and a rule-based energy management strategy have been proposed, and the electric energy and hydrogen consumptions required to achieve the target driving range for roll-on and roll-off operations have been estimated. The results are promising, showing that the hybrid electric vehicle is capable of achieving excellent energy performances, by means of an efficient use of the fuel cell. An overall amount of roughly 12 kg of hydrogen is estimated to be required to accomplish the most demanding port operation, and meet the target of 6 h of continuous operation. Also, the vehicle powertrain ensures an adequate all-electric range, which is between approximately 1 and 2 h depending on the specific port operation. Potentially, the hydrogen-fueled yard truck is expected to lead to several benefits, such as local zero emissions, powertrain noise elimination, reduction of the vehicle maintenance costs, improving of the energy management, and increasing of operational efficiency

Ovine Catarrhal fever (bluetongue): Analysis of Culicoides species in seropositive farms

Bluetongue (BT) is an orbiviral disease of wild and domestic ruminants, mainly sheep. In Sicily, the first Bluetongue outbreak occurred in October 2000; there have been 76 recorded outbreaks so far. The National Surveillance Plan, based on European Union Commission Decision 138/2001/CE, establishes serological and entomological surveys. This plan consists of controls of seronegative cattle, called 'sentry' as indicators for the presence and circulation of virus in defined areas. To check the seroconversions, the regional territory has been subdivided in 400 km2 areas including 58 seronegative cattle, periodically checked by serological tests. All positive sera have been tested to detect the specific serotype by the National Reference Centre for Exotic Diseases (CESME) at the Istituto Zooprofilattico Sperimentale Abruzzo e Molise in Teramo (IZS Teramo). Moreover, entomological surveillance has been implemented in seropositive herds, to investigate the presence of insect vectors belonging to Culicoides genus. The goal of the present communication is to report on the different species of Culicoides found in the farms with Bluetongue virus and to investigate on the probable role of new competent vectors. This paper concerns data analysis of 581 light-trap catches collected in 321 farms from 2003 to 2008. We observed that 82% of checked farms were positive for Culicoides spp., and only 10% of the farms were positive for Culicoides imicola. © 2010 Blackwell Verlag GmbH

Assessment of a Hydrogen-Fueled Heavy-Duty Yard Truck for Roll-On and Roll-Off Port Operations

The port-logistic industry has a significant impact on the urban environment nearby ports and on the surrounding coastal areas. This is due to the use of large auxiliary power systems on ships operating during port stays, as well as to the employment of a number of fossil fuel powered road vehicles required for port operations. The environmental impact related to the use of these vehicles is twofold: on one hand, they contribute directly to port emissions by fuel consumption; on the other hand, they require some of the ship auxiliary systems to operate intensively, such as the ventilation system, which must operate to remove the pollutants produced by the vehicle engines inside the ship. The pathway to achieve decarbonization and mitigation of energy use in ports involves therefore the adoption of alternative and cleaner technology solutions for the propulsion systems of such port vehicles. This paper presents the performance analysis of a hydrogen powered cargo-handling vehicle for roll-on and roll-off port operations in a real case scenario. The fuel cell/battery hybrid powertrain of the vehicle has been previously designed by the authors. On the base of real data acquired during an on-field measurement campaign, and by means of a validated numerical model of the vehicle dynamics, different mission profiles are defined, in terms of driving and duty cycles, in order to represent typical port operations. A rule-based energy management strategy is then used to estimate the energy and hydrogen consumptions required by the vehicle and to assess its suitability to accomplish the defined target port operations. Outputs from this study show the potential of the proposed solution to take the place, in a foreseeable future, of conventional Diesel-engine vehicles, today commonly used in port logistics, towards a zero-emission scenario

Quality controls for cell cultures: identification of interspecies cross-contamination by PCR-RFLP analysis of the cytochrome b gene

Cross-contaminations of a cell line with cells of different species represent a potential risk in laboratories handling human and animal cells. Therefore, it is necessary to control such contaminations. Tests based on mitochondrial DNA (mtDNA) are used in forensic analysis, phylogenetic studies and in food authentication. However, the use of mtDNA in quality controls of cell cultures is recent. Mitochondrial sequence differences of closely related animal species are five- to tenfold higher than those of nuclear genes. On the contrary, intraspecies variation in mitochondrial sequences is low in most animal species. Moreover, each cell contains 100–10.000 mitochondrial genomes. The amount of mtDNA is greater than nuclear DNA, so that mtDNA can be analyzed also from small or partially degraded samples. In the present study, a method based on a PCR-Restriction Fragment Length Polymorphism (RFLP) analysis of the mitochondrial cytochrome b gene was used (2). This gene has some stable sequences which are recognized from universal primers and some variable sequences used for animal species identification by PCR-RFLP method

Canine Mesenchymal Stem Cells from visceral and subcutaneuous adipose tissue for cell-based therapy

This study compared some characteristics of canine Adipose tissue-Derived Mesenchymal Stem Cells (cAD-MSCs) from subcutaneous and visceral fat. These findings were directed to obtain high quantity and quality cAD-MSCs for clinical cell-based therapy

An innovative method for the detection of contaminant viral genome in cell cultures

The use of cell cultures involves different fields of biology, from diagnosis to research. Moreover, technologies based on animal cells represent a useful tool to the development of biological products for the prophylaxis and therapy in humans and animals. Therefore, it is necessary to perform quality controls, including virological tests. Several tests performed in research laboratories are able to discriminate one or more viral species, but it is not possible to demonstrate the presence of contaminant viral genome with one non-specific method. The aim of this work consisted on the realization of a biomolecular method able to detect and to identify by sequencing extraneous viral genome in cell cultures of animal and human origin in the absence of any specific information about the virus

Characterization of Colletotrichum strains associated with olive anthracnose in Sicily

Anthracnose caused by Colletotrichum spp. is the most damaging olive fruit disease in many countries, including Italy. This disease has been sporadically detected in Sicily, but new agronomic practices can increase risk of olive anthracnose in this region. An etiological study of the disease focused on local olive cultivars growing at the International Olive Germplasm Collection (IOGC) in Villa Zagaria, Enna, Sicily has been undertaken. During 2018 and 2019, 137 Colletotrichum strains were isolated from olives. Colony morphology, conidium characteristics, and multilocus sequence analyses aided identification of three species: C. acutatum (affecting 70% of symptomatic olives), C. gloeosporioides, and C. cigarro. Three C. acutatum strains (B1316, P77, and P185), and one stram of each C. gloeosporioides (C2.1) and C. cigarro (Perg6B) were evaluated for pathogenicity on olive fruits from 11 Sicilian cultivars, known for their high-quality oil. Differences in virulence were detected among strains and their pathogenicity to the cultivars. The C. acutatum isolates were more virulent than those of C. gloeosporioides or C. cigarro. The Sicilian olive cultivars Cavaliera, Carolea, Calatina, and Nocellara del Belice were the most susceptible to the pathogen, while the cultivars Biancolilla and Nocellara Etnea were the most tolerant. Cultivar response under field conditions showed that anthracnose severity and fruit-rot incidence were positively correlated. This is the first report of C. acutatum and C. cigarro affecting olive trees in Sicily. Control measures for anthracnose depend on accurate characterization of the etiological agents and host cultivar resistance