Incidence and Molecular Typing of Vibrio parahaemolyticus from Tiger Shrimp Culture Environments along the Southwest Coast of India

Vibrio parahaemolyticus is one of the most prevalent food-borne pathogens along the southwest coast of India, where marine foods are frequently consumed. Shrimp (Penaeus monodon) and environmental samples were collected from aquaculture farms located in and around Cochin. Confirmation of the biochemically identified strains with species-specific toxR gene and detection of virulent genes viz., tdh and trh was performed by polymerase chain reaction (PCR). The phenotypic markers for the presence of tdh and trh genes were assayed by Kanagawa phenomenon and urease activity, respectively. Protease activity was examined to identify other potential virulence factors. After phenotypic characterization of bacterial strains fingerprinting of genomic DNA was carried by various typing methods, viz., random amplified polymorphic DNA (RAPD), enterobacterial repetitive intergenic consensus sequence (ERIC), repetitive extragenic palindromic sequence (REP), and ribosomal gene spacer sequence (RS) PCR methods to assess the genetic diversity within the isolates. Eighteen percent of the samples were found positive for the incidence of V. parahaemolyticus by biochemical protocols and toxR (368 bp) targeted PCR. PCR analyses revealed 1% of the samples positive for tdh (269 bp) and trh (500 bp) gene. RAPD analysis revealed clustering of toxigenic strains into a single group. Cluster analysis revealed the conglomeration of isolates into two, five, and seven major groups using RS, ERIC, and REP PCR methods, respectively. RS PCR generated fewer amplified bands compared to REP and ERIC PCR methods, thus giving scope for higher discrimination. Moreover, RS PCR patterns were more discernible visually from other patterns, suggesting RS PCR as a considerably practical method for routine use

Note on a fishery of Parapenaeus longipes along the Veraval Coast

Parapenaeus longipes, commonly known as “Flamingo shrimp” and locally as “Bhoonsi”,it is not known to occur exclusively as fishery in any area of India and abroad

Occurrence and distribution of virulent strains of Vibrio parahaemolyticus in seafoods marketed from Cochin (India)

This study was aimed for the detection of Vibrio parahaemolyticus by biochemical and molecular methods in seafood samples collected from the markets of Cochin located at the southwest coast of India.Atotal of seventy-two V. parahaemolyticus cultures were isolated by selecting sucrose and cellobiose non-fermenting colonies. All the biochemically confirmed strains were found to have 368-bp toxR gene fragment, while an additional 24% of the samples were confirmed as V. parahaemolyticus by toxR based polymerase chain reaction (PCR) from enrichment broths

Growth Kinetics and Survival of Urease positive and negative Strains of Vibrio parahaemolyticus

Two each of urease positive and negative Vibrio parahaemolyticus strains along with their respective type strains were used for studying the growth kinetics and survival at various temperatures, NaCl concentrations and pH. Maximum growth was obtained at 37°C for both urease positive and negative isolates. The urease positive and negative strains were found to grow only at pH 7 and 9 among the pH's tested. Urease negative isolates were found to grow between 3% and 8% NaCI while urease positives could grow between 3% and 10% NaCI

Relevance vector machine based fault classification in wind energy conversion system

This Paper is an attempt to develop the multiclass classification in the Benchmark fault model applied on wind energy conversion system using the relevance vector machine (RVM). The RVM could apply a structural risk minimization by introducing a proper kernel for training and testing. The Gaussian Kernel is used for this purpose. The classification of sensor, process and actuators faults are observed and classified in the implementation. Training different fault condition and testing is carried out using the RVM implementation carried out using Matlab on the Wind Energy Conversion System (WECS). The training time becomes important while the training is carried out in a bigger WECS, and the hardware feasibility is prime while the testing is carried out on an online fault detection scenario. Matlab based implementation is carried out on the benchmark model for the fault detection in the WECS. The results are compared with the previously implemented fault detection technique and found to be performing better in terms of training time and hardware feasibility

Distribution of Plesionika semilaevis along the southwest coast of India

The commercial deep-sea caridean shrimp Plesionika martia (A. Milne-Edwards, 1883) has long been recorded from India and constitutes an important part of catches of the deep-sea shrimp fisheries. A recent survey in some deep-sea fishing harbours along the south-west coast of India, however, revealed that all material previously reported as 'P. martia' is actually a misidentification of its closely related species Plesionika semilaevis Spence Bate, 1888

Record size of (Carrot tongue sole) Cynoglossus dubius, caught off Veraval, Gujarat

The largest ever recorded tongue sole (Cynoglossus dubius) from Indian waters was landed by a monofilament gillnet at Veraval Fishing Harbour in Gujarat

Isolation and characterization of Vibrio parahaemolyticus from seafoods along the southwest coast of India

The work was aimed to study the microbial quality of the seafood sold in the domestic markets and incidence of Vibrio parahaemolyticus. Samples comprising of shellfish, finfish, and cephalopodswere collected fromvarious fish markets in and around Cochin. Presumed V. parahaemolyticus were identified by standard biochemical tests, and further confirmed by polymerase chain reaction targeting species-specific tl gene (450 bp)

A new record of deep-sea caridean shrimp Heterocarpus chani (Decapoda: Pandalidae) from the southern coast of India

Heterocarpus genus belong to the family Pandalidae (Decapoda, Caridea) which are common in deeper waters. Some species are of commercial value or fishery potential by their large size. They are characterized by rostrum armed with teeth on both margins; carapace with postrostral carina extending nearly to posterior margin and with 1 or more longitudinal lateral carinae; pereopods with 2nd pair distinctly unequal and dissimilar. The species Heterocarpus chani was recorded from various fishing harbours on the south coast (Sakthikulangara Fishing Harbour; Kalamuku Landing centre (Kerala) and the Thoothukudi and Nagapattinam Fisheries Harbours Tamil Nadu) from the deep-sea catches taken at depth of 200 – 350 m

Plesionika reflexa - a new record of deep-sea caridean shrimp from the southwest coast of India

Plesionika reflexa, Chace, 1985 (Decapoda: Pandalidae) was recorded from the catch of deep sea shrimp trawlers operated at a depth of 200-300 m off Sakthikulangara (8°56’60.78"N / 76°32’ 34.27"E), Kollam south-west coast of India. The ovigerous female [Carapace length (CL): 15mm; Rostrum length (RL): 28mm] was reddish in colour with dark red ring formation in posterior abdomen