Biologic Treatments for Sports Injuries II Think Tank-Current Concepts, Future Research, and Barriers to Advancement, Part 2:Rotator Cuff

Rotator cuff tears are common and result in considerable morbidity. Tears within the tendon substance or at its insertion into the humeral head represent a considerable clinical challenge because of the hostile local environment that precludes healing. Tears often progress without intervention, and current surgical treatments are inadequate. Although surgical implants, instrumentation, and techniques have improved, healing rates have not improved, and a high failure rate remains for large and massive rotator cuff tears. The use of biologic adjuvants that contribute to a regenerative microenvironment have great potential for improving healing rates and function after surgery. This article presents a review of current and emerging biologic approaches to augment rotator cuff tendon and muscle regeneration focusing on the scientific rationale, preclinical, and clinical evidence for efficacy, areas for future research, and current barriers to advancement and implementation

Molecular and cellular mechanisms underlying the evolution of form and function in the amniote jaw.

The amniote jaw complex is a remarkable amalgamation of derivatives from distinct embryonic cell lineages. During development, the cells in these lineages experience concerted movements, migrations, and signaling interactions that take them from their initial origins to their final destinations and imbue their derivatives with aspects of form including their axial orientation, anatomical identity, size, and shape. Perturbations along the way can produce defects and disease, but also generate the variation necessary for jaw evolution and adaptation. We focus on molecular and cellular mechanisms that regulate form in the amniote jaw complex, and that enable structural and functional integration. Special emphasis is placed on the role of cranial neural crest mesenchyme (NCM) during the species-specific patterning of bone, cartilage, tendon, muscle, and other jaw tissues. We also address the effects of biomechanical forces during jaw development and discuss ways in which certain molecular and cellular responses add adaptive and evolutionary plasticity to jaw morphology. Overall, we highlight how variation in molecular and cellular programs can promote the phenomenal diversity and functional morphology achieved during amniote jaw evolution or lead to the range of jaw defects and disease that affect the human condition

Studies on the virome of the entomopathogenic fungus Beauveria bassiana reveal novel dsRNA elements and mild hypervirulence.

© 2017 Kotta-Loizou, Coutts. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Kotta-Loizou I, Coutts RHA (2017) 'Studies on the Virome of the Entomopathogenic Fungus Beauveria bassiana Reveal Novel dsRNA Elements and Mild Hypervirulence', PLoS Pathogens, 13(1): e1006183. doi:10.1371/journal.ppat.1006183The entomopathogenic fungus Beauveria bassiana has a wide host range and is used as a biocontrol agent against arthropod pests. Mycoviruses have been described in phytopathogenic fungi while in entomopathogenic fungi their presence has been reported only rarely. Here we show that 21.3% of a collection of B. bassiana isolates sourced from worldwide locations, harbor dsRNA elements. Molecular characterization of these elements revealed the prevalence of mycoviruses belonging to the Partitiviridae and Totiviridae families, the smallest reported virus to date, belonging to the family Narnaviridae, and viruses unassigned to a family or genus. Of particular importance is the discovery of members of a newly proposed family Polymycoviridae in B. bassiana. Polymycoviruses, previously designated as tetramycoviruses, consist of four non-conventionally encapsidated capped dsRNAs. The presence of additional non-homologous genomic segments in B. bassiana polymycoviruses and other fungi illustrates the unprecedented dynamic nature of the viral genome. Finally, a comparison of virus-free and virus-infected isogenic lines derived from an exemplar B. bassiana isolate revealed a mild hypervirulent effect of mycoviruses on the growth of their host isolate and on its pathogenicity against the greater wax moth Galleria mellonella, highlighting for the first time the potential of mycoviruses as enhancers of biocontrol agents.Peer reviewedFinal Published versio

Euclid preparation. III. Galaxy cluster detection in the wide photometric survey, performance and algorithm selection

Galaxy cluster counts in bins of mass and redshift have been shown to be a competitive probe to test cosmological models. This method requires an efficient blind detection of clusters from surveys with a well-known selection function and robust mass estimates, which is particularly challenging at high redshift. The Euclid wide survey will cover 15 000 deg2 of the sky, avoiding contamination by light from our Galaxy and our solar system in the optical and near-infrared bands, down to magnitude 24 in the H-band. The resulting data will make it possible to detect a large number of galaxy clusters spanning a wide-range of masses up to redshift ∼2 and possibly higher. This paper presents the final results of the Euclid Cluster Finder Challenge (CFC), fourth in a series of similar challenges. The objective of these challenges was to select the cluster detection algorithms that best meet the requirements of the Euclid mission. The final CFC included six independent detection algorithms, based on different techniques, such as photometric redshift tomography, optimal filtering, hierarchical approach, wavelet and friend-of-friends algorithms. These algorithms were blindly applied to a mock galaxy catalog with representative Euclid-like properties. The relative performance of the algorithms was assessed by matching the resulting detections to known clusters in the simulations down to masses of M₂₀₀ ∼ 10^(13.25) M⊙. Several matching procedures were tested, thus making it possible to estimate the associated systematic effects on completeness to 80% completeness for a mean purity of 80% down to masses of 10¹⁴ M⊙ and up to redshift z = 2. Based on these results, two algorithms were selected to be implemented in the Euclid pipeline, the Adaptive Matched Identifier of Clustered Objects (AMICO) code, based on matched filtering, and the PZWav code, based on an adaptive wavelet approach