The Study of Sorption of the Milk Ionized Calcium by Sodium Alginate

The possibility of regulation of defatted cow milk salt composition (fat content– 0,05 %) with the use of sodium alginate as a natural ion-exchanger was studied. Realization of the properties of sodium alginate as to the bounding of calcium ions allows receive the systems on the base of cow milk, stable in time and at the thermal processing. The studies established the influence of technological factors on the sorption of ionized calcium by the solution of complex-creator by sodium alginate. It was established, that the important factors that influence the process are the active acidity and conditions of the process, namely, the phased addition of sorbent that favors the equal speed of process and, as the result, the equal speed of sorption during the whole process. At the same time the study of influence of sorption area and temperature demonstrates that these factors are not determining ones in this process. It was established, that rationalization of parameters of ionized calcium sorption results in the raise of thermal stability of defatted cow milk and the systems on its base. Materials, given in the work, are the base for elaboration and introduction of technology of dessert production (ice-cream, creams, puddings, cocktails and so on), in which composition the colloid stability is provided at the joint use of milk and fruit-berry raw material that can be used in food industry

The coasts we live in: can there be a single definition for a coastal zone?

Throughout the history of humankind, people have settled along seashores. The gradual accumulation of population and industrial activity in coastal areas has created preconditions for coastalisation - the movement of people and socio-economic activity to marine coasts. To date, coastal areas have a higher rate of economic development, fostering migration and an influx of capital across the globe. Scholars and policymakers voice concerns about the asymmetry of regional development and the increasing anthropogenic impact on the coastal ecosystem. It reinforces the importance of coastal zone management. In this study, we use an example of the Baltic region to identify the coastalisation patterns in the Baltic region and answer the question, whether there can be a single definition of the coastal zone of the Baltic region. According to a broad definition, the Baltic macro-region is nearly all coastal and, consequently, all settlements are influenced by the coastalisation effect. We have studied urban population dynamics in 128 cities of 45 coastal regions through the lens of various characteristics of a coastal city - the distance from the sea (10, 50, 100, and 150 km), location in a coastal region (NUTS 2), availability of a port and its primary maritime activity (tankers, cargo, fishing, passenger, recreational vessels and others). The research results suggest that despite the strong coherence of the Baltic region countries, there should not be a single delimitation approach to defining the coastal zone. Overall, the most active marine economic processes occur in the zone up to 10 km from the seacoast and 30 km from ports and port infrastructure. However, in the case of Sweden, Poland, and Latvia, the coastal zone can be extended to 50 km, and in Germany - up to 150 km inland

ENDOTHELIAL PROTECTIVE ACTIVITY OF 2,6-DIISOBORNYL-4-METHYLPHENOL IN A MODEL OF MYOCARDIAL ISCHEMIA/REPERFUSION IN RATS

Objective: Our research focuses on the endothelial protective effects of 2,6-diisobornyl-4-methylphenol. Its effect was revealed while studying rats experiencing myocardial ischemia/reperfusion. The research results demonstrated that there are significant disturbances in the vascular endothelium manifested by a decrease in the vasodilating activity and antiplatelet properties of 2,6-diisobornyl-4-methylphenol. Methods: We designed our own model of myocardial ischemia/reperfusion and applied it to 52 adult outbred Wistar males. We employed some methods of hemostasiological research such as thromboelastography to determine the antiplatelet activity of the vascular wall, G. Born nephelometric method to study platelet aggregation, phase contrast microscopy to count platelet counts in blood plasma, measurement of intra-arterial pressure to study the endothelial vasodilating function, and calculated the endothelial dysfunction coefficient in rats. Results: Preventive intragastric injection of 2,6-diisobornyl-4-methylphenol (100 mg/kg, 3 days before and 5 days after reproducing the myocardial ischemia/reperfusion model) increased the antiplatelet activity of the vascular endothelium in rats by 37% compared to the endothelium of the abdominal aorta segment of untreated animals. Moreover, 2,6-diisobornyl-4-methylphenol decreased the endothelial dysfunction coefficient by 43% in comparison with the value in the control group. Conclusion: 2,6-diisobornyl-4-methylphenol has an endothelial protective effect proved by its ability to increase antiplatelet properties of the endothelium and decrease the endothelial dysfunction coefficient. The revealed endothelial protective properties of 2,6-diisobornyl-4-methylphenol can be regarded as one of the potential mechanisms of cardioprotective activity of the drug

Comparison of digital image analysis methods for morphometric characterization of soil aggregates in thin sections

The purpose of this study was to investigate the applicability of semiautomatic segmentation methods for obtaining and evaluating morphometric parameters of soil aggregates in artificially prepared loose samples in soil thin sections. The object of the research is typical arable Chernozem. The aggregates were separated by wet sieving method from loose sample of upper 10 cm of the plowing horizon after erosion by a model shallow water flow on a large erosion tray. The aggregates, loosely scattered on the glass and fixed with polyester resin, were used to produce the thin sections. Images of the thin sections were taken under a polarizing microscope and then were processed using two methods compared: Adobe Photoshop + CTan and Thixomet Pro. Data on morphometric parameters of aggregates were obtained: the shape factor, the degree of roundness and the coefficient of aggregate surface roughness. The convergence of the results obtained using Photoshop + CTan by three researchers was evaluated by comparing samples using the Student's test and the Mann-Whitney test. The convergence of the averaged results obtained using Photoshop + CTan and the results obtained using Thixomet Pro was evaluated using the Mann - Whitney test. No significant differences were found between the parameters of the same aggregates obtained using a combination of Adobe Photoshop and CTan programs by different researchers. No significant differences were found between the parameters of the same aggregates obtained by the compared methods. So, one can conclude that the reliability of determining the morphometric parameters of soil aggregates using Thixomet Pro is comparable to the reliability of results when working with images of sectionsin CTan after binarization in Adobe Photoshop. The method of obtaining data on morphometric parameters of soil aggregates using Thixomet Pro completely eliminates the possibility of subjective error, shows a high degree of automation, reproducibility and reliability of the results obtained, and is faster

Drought resistance of introgressive spring common wheat lines with genetic material of tall wheatgrass

Background. To breed drought-resistant cultivars of common wheat1 (Triticum aestivum L.), it is important to use the gene pools of its relatives, including tall wheatgrass Thinopyrum ponticum (Podpěra) Z.-W. Liu & R.- C. Wang (= Agropyron elongatum (Host) Beauv.).   Materials and methods. The introgressive lines of spring common wheat with T. ponticum genetic material and standard cultivars were studied in the field in the southern forest-steppe of Western Siberia using generally recognized methods. The ecological plasticity of cultivars and introgressive lines by grain yield and yield components was calculated according to the method of S. A. Eberhart and W. A. Russell. During the research period, there was a prolonged drought in 2012, and irregular short severe droughts occurred in 2013, 2014, and 2017.   Results. An analysis of the ecological plasticity of standard cultivars adapted to the regional conditions showed that cv. ‘Pamyati Azieva’ corresponded to the extensive type, and cvs. ‘Duet’, and ‘Erythrospermum 59’ corresponded to the intensive type. Under drought conditions, the grain yield of cv. ‘Pamyati Azieva’ was determined by the stable development of productive tiller number, seed number and grain yield per main ear, but plasticity in 1000 grain weight was observed. Cvs. ‘Duet’ and ‘Erythrospermum 59’ showed ecological plasticity due to the adaptive development of two or three yield components. Introgressive lines exceeded the standard cultivars in grain yield (1.1–2.2 times) in dry seasons. Five lines were similar to cv. ‘Pamyati Azieva’ in plasticity and stability, and under drought conditions they demonstrated high and stable development of three or four yield components. The intensive lines formed their yield due to the compensatory development of three yield components in different combinations.   Conclusion. Introgressive lines with T. ponticum genetic material are valuable for breeding spring common wheat cultivars with various drought-adaptation mechanisms

Mutational re-modeling of di-aspartyl intramembrane proteases: uncoupling physiologically-relevant activities from those associated with Alzheimer\u27s disease

The intramembrane proteolytic activities of presenilins (PSEN1/PS1 and PSEN2/PS2) underlie production of beta-amyloid, the key process in Alzheimer\u27s disease (AD). Dysregulation of presenilin-mediated signaling is linked to cancers. Inhibition of the gamma-cleavage activities of PSENs that produce Abeta, but not the epsilon-like cleavage activity that release physiologically essential transcription activators, is a potential approach for the development of rational therapies for AD. In order to identify whether different activities of PSEN1 can be dissociated, we designed multiple mutations in the evolutionary conserved sites of PSEN1. We tested them in vitro and in vivo assays and compared their activities with mutant isoforms of presenilin-related intramembrane di-aspartyl protease (IMPAS1 (IMP1)/signal peptide peptidase (SPP)). PSEN1 auto-cleavage was more resistant to the mutation remodeling than the epsilon-like proteolysis. PSEN1 with a G382A or a P433A mutation in evolutionary invariant sites retains functionally important APP epsilon- and Notch S3- cleavage activities, but G382A inhibits APP gamma-cleavage and Abeta production and a P433A elevates Abeta. The G382A variant cannot restore the normal cellular ER Ca(2+) leak in PSEN1/PSEN2 double knockout cells, but efficiently rescues the loss-of-function (Egl) phenotype of presenilin in C. elegans. We found that, unlike in PSEN1 knockout cells, endoplasmic reticulum (ER) Ca(2+) leak is not changed in the absence of IMP1/SPP. IMP1/SPP with the analogous mutations retained efficiency in cleavage of transmembrane substrates and rescued the lethality of Ce-imp-2 knockouts. In summary, our data show that mutations near the active catalytic sites of intramembrane di-aspartyl proteases have different consequences on proteolytic and signaling functions

Early stages of the evolution of chernozems under forest vegetation (Belgorod Oblast)

We studied automorphic forest-steppe Luvic and Haplic Chernozems (Siltic/Clayic, Pachic) of the southern part of the Central Russian Upland (Belgorod region), which were covered with broadleaved forest vegetation at different times (from 25 to 75 years ago). The studies were carried out on an overgrowing fallow and the adjacent maple-ash shelterbelt and on an area of growth of a natural oak forest towards the virgin meadow stepp

Deficiency in origin licensing proteins impairs cilia formation: implications for the aetiology of meier-gorlin syndrome

Mutations in ORC1, ORC4, ORC6, CDT1, and CDC6, which encode proteins required for DNA replication origin licensing, cause Meier-Gorlin syndrome (MGS), a disorder conferring microcephaly, primordial dwarfism, underdeveloped ears, and skeletal abnormalities. Mutations in ATR, which also functions during replication, can cause Seckel syndrome, a clinically related disorder. These findings suggest that impaired DNA replication could underlie the developmental defects characteristic of these disorders. Here, we show that although origin licensing capacity is impaired in all patient cells with mutations in origin licensing component proteins, this does not correlate with the rate of progression through S phase. Thus, the replicative capacity in MGS patient cells does not correlate with clinical manifestation. However, ORC1-deficient cells from MGS patients and siRNA-mediated depletion of origin licensing proteins also have impaired centrosome and centriole copy number. As a novel and unexpected finding, we show that they also display a striking defect in the rate of formation of primary cilia. We demonstrate that this impacts sonic hedgehog signalling in ORC1-deficient primary fibroblasts. Additionally, reduced growth factor-dependent signaling via primary cilia affects the kinetics of cell cycle progression following cell cycle exit and re-entry, highlighting an unexpected mechanism whereby origin licensing components can influence cell cycle progression. Finally, using a cell-based model, we show that defects in cilia function impair chondroinduction. Our findings raise the possibility that a reduced efficiency in forming cilia could contribute to the clinical features of MGS, particularly the bone development abnormalities, and could provide a new dimension for considering developmental impacts of licensing deficiency

The Toxic effects of the metals connected with changes of an immune profile of children from areas with raised technogenic loading of Sverdlovsk region

Results of inspection of 2096 children which lived in the territories carried to categories of risk on development of ecologically determined pathology Are presented. Children went on inspection and treatment after special selection taking into account a total rank on toxic loading, clinical indications. The estimation of efficiency of treatment included dynamics of the maintenance of heavy metals in blood. Results of clinic and laboratory researches were compared with made blood analyses on definition of concentration of heavy metals. The method has been applied to the analysis of interrelations of parameters of a homeostasis in comparison groups with use of F-criterion of communication. Considerable changes in immunity are formation basis pathologies of children from territories with bad ecology. Proofs of participation Pb. As, Си, Ni, Cr, Zn, Cd in formation of adverse factors of the anamnesis and a deviation of an immune profile of children are received. Efficiency of the spent treatment was accompanied by authentic decrease in concentration of heavy metals in blood of patients.Представлены результаты обследования 2096 детей, которые проживали на территориях, отнесённых к категориям риска по развитию экологически детерминированной патологии. Дети направлялись на обследование и лечение после специального отбора с учетом суммарного ранга по токсической нагрузке, клинических показаний. Оценка эффективности лечения включала динамику содержания тяжелых металлов в крови. Результаты клинико-лабораторных исследований сопоставлялись с данными анализов крови на определение концентрации тяжелых металлов. Для анализа взаимосвязей параметров гомеостаза в группах сравнения был применен метод с использованием F-критерия связи. Значительные нарушения в системе клеточного, гуморального иммунитета и системы фагоцитоза являются основой формирования полиорганной патологии у детей из экологически неблагополучных территорий. Получены доказательства участия Pb, As, Си, Ni, Сг, Zn, Cd в формировании неблагоприятных факторов анамнеза и отклонения иммунного профиля у детей. Эффективность проведённого лечения сопровождалась достоверным снижением концентрации тяжелых металлов в крови пациентов