Measurement of gas phase characteristics using amonofibre optical probe in a three-phase flow

The study of gas–liquid–solid systems structure requires reliable measurement tools. In this paper, preliminary results on the potential use of a monofibre optical probe to investigate such flow are presented. This probe, manufactured at LEGI, allows the simultaneous measurement of the gas phase residence time and gas phase velocity. This specificity makes this probe more interesting than classical single tip probes (which measure only the gas residence time) or double tip probes (which are more intrusive). Although extensively used in two-phase gas–liquid, this probe was never used in gas–liquid–solid systems. First, the probe signal response is studied for three-phase flow conditions in the presence of solids. Results show that for soft solids, the probe tips can be contaminated when the probe pierces the solid. The signal processing procedure was modified accordingly to take into account these events. Second, the probe results are validated by comparing global results (global void fraction, gas flowrate) deduced from profile measurements with measurements performed by independent means. Lastly, void fraction profiles and interfacial area are studied more in detail. Depending on the solid loading, these profiles exhibit different behaviours. These features are associated to characteristics of the flow such as the transition from an homogeneous regime to an heterogenous regime, and are consistent with global observation performed by independent means. This demonstrates the ability of the probe to connect local information to the global behaviour and structure of the flow.Fundação para a Ciência e a Tecnologia (FCT

Customization of an optical probe device and validation of a signal processing procedure to study gas-liquid-solid flows. Application to a three-phase internal-loop Gas-lift Bioreactor

The study of local hydrodynamic properties of three-phase bioreactors in biotechnology processes is of great importance, mainly because of the complex interaction between bioreactor and microorganisms. However, classical techniques used for measuring local hydrodynamic properties such as single needle probes are mainly limited to two-phase flows. In this work it was developed and validated a new system, based on the customization of an optical probe initially designed in LEGI. The necessity of a new system was due to the agglomeration of the solid-phase (spent grains which are used as the micro-organisms carrier for the targeted application) around the optical tip, which influences the measurements. This new system allows for the measurement of the main local gas-phase properties in a complex gas-liquid-solid mixture. The new system was first validated for air-water system in an internal loop gas-lift reactor and then applied to a spent grains-air-water mixture at low solid load in an internal gas lift reactor. In addition, experiments using complementary techniques (as high speed camera and PIV) were performed that allowed for the validation of the new system and the explanation of possible physical mechanisms that are underlying on this multiphase system. The system developed has the potential for improvement and use in several biotechnology applications.The authors gratefully acknowledge the financial support from FCT (Fundacao para a Ciencia e Tecnologia, SFRH/BD/37082/2007 and SFRH/BPD/45637/2008)

Sisclima: creation d'un systeme d'informations climatiques informatisees en region amazonienne.

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SYNERGISTIC ON AUXIN AND CYTOKININ 1 positively regulates growth and attenuates soil pathogen resistance

Plants as non-mobile organisms constantly integrate varying environmental signals to flexibly adapt their growth and development. Local fluctuations in water and nutrient availability, sudden changes in temperature or other abiotic and biotic stresses can trigger changes in the growth of plant organs. Multiple mutually interconnected hormonal signaling cascades act as essential endogenous translators of these exogenous signals in the adaptive responses of plants. Although the molecular backbones of hormone transduction pathways have been identified, the mechanisms underlying their interactions are largely unknown. Here, using genome wide transcriptome profiling we identify an auxin and cytokinin cross-talk component; SYNERGISTIC ON AUXIN AND CYTOKININ 1 (SYAC1), whose expression in roots is strictly dependent on both of these hormonal pathways. We show that SYAC1 is a regulator of secretory pathway, whose enhanced activity interferes with deposition of cell wall components and can fine-tune organ growth and sensitivity to soil pathogens. Cytokinin and auxin are two major hormonal regulators of plant growth. Here the authors identify SYAC1, a gene that is synergistically activated by the two hormones being applied together, and show that it is required for normal growth while negatively impacting pathogen resistance

La lutte contre l'envasement des etangs landais par epandages de craie

National audienc

Sisfito: um sistema de administração de herbário.

Com o objetivo de subsidiar os trabalhos de identificação do material botânico, está em fase adiantada de concepção, um arquivo de dados complementares inerentes ~s características morfológicas das plantas

La lutte contre l'envasement

National audienc

Beta-N-methylamino-L-alanine (BMAA) and isomers : distribution in different food web compartments of Thau lagoon, French Mediterranean Sea

The neurotoxin BMAA (beta-N-methylamino-L-alanine) and its isomer DAB (2,4-diaminobutyric acid) have been detected in seafood worldwide, including in Thau lagoon (French Mediterranean Sea). A cluster of amyotrophic lateral sclerosis (ALS), a neurodegenerative disease associated with BMAA, has also been observed in this region. Mussels, periphyton (i.e. biofilms attached to mussels) and plankton were sampled between July 2013 and October 2014, and analyzed using HILIC-MS/MS. BMAA, DAB and AEG (N-(2-aminoethyl)glycine) were found in almost all the samples of the lagoon. BMAA and DAB were present at 0.58 and 0.83, 2.6 and 33, 4.0 and 7.2 mu g g(-1) dry weight in plankton collected with nets, periphyton and mussels, respectively. Synechococcus sp., Ostreococcus tauri, Alexandrium catenella and eight species of diatoms were cultured and screened for BMAA and analogs. While Synechococcus sp., O. tauri and A. catenella did not produce BMAA under our culture conditions, four diatoms species contained both BMAA and DAB. Hence, diatoms may be a source of BMAA for mussels. Unlike other toxins produced by microalgae, BMAA and DAB were detected in significant amounts in tissues other than digestive glands in mussels