Photoactivatable metal complexes : from theory to applications in biotechnology and medicine

This short review highlights some of the exciting new experimental and theoretical developments in the field of photoactivatable metal complexes and their applications in biotechnology and medicine. The examples chosen are based on some of the presentations at the Royal Society Discussion Meeting in June 2012, many of which are featured in more detail in other articles in this issue. This is a young field. Even the photochemistry of well-known systems such as metal–carbonyl complexes is still being elucidated. Striking are the recent developments in theory and computation (e.g. time-dependent density functional theory) and in ultrafast-pulsed radiation techniques which allow photochemical reactions to be followed and their mechanisms to be revealed on picosecond/nanosecond time scales. Not only do some metal complexes (e.g. those of Ru and Ir) possess favourable emission properties which allow functional imaging of cells and tissues (e.g. DNA interactions), but metal complexes can also provide spatially controlled photorelease of bioactive small molecules (e.g. CO and NO)—a novel strategy for site-directed therapy. This extends to cancer therapy, where metal-based precursors offer the prospect of generating excited-state drugs with new mechanisms of action that complement and augment those of current organic photosensitizers

Excited-state dynamics of a two-photon-activatable ruthenium prodrug

We present a new approach to investigate how the photodynamics of an octahedral ruthenium(II) complex activated through two-photon absorption (TPA) differ from the equivalent complex activated through one-photon absorption (OPA). We photoactivated a RuII polypyridyl complex containing bioactive monodentate ligands in the photodynamic therapy window (620–1000 nm) by using TPA and used transient UV/Vis absorption spectroscopy to elucidate its reaction pathways. Density functional calculations allowed us to identify the nature of the initially populated states and kinetic analysis recovers a photoactivation lifetime of approximately 100 ps. The dynamics displayed following TPA or OPA are identical, showing that TPA prodrug design may use knowledge gathered from the more numerous and easily conducted OPA studies

New HI-detected Galaxies in the Zone of Avoidance

We present the first results of a blind HI survey for galaxies in the southern Zone of Avoidance with a multibeam receiver on the Parkes telescope. This survey is eventually expected to catalog several thousand galaxies within Galactic latitude |b|<5 degrees, mostly unrecognised before due to Galactic extinction and confusion. We present here results of the first three detections to have been imaged with the Australia Telescope Compact Array (ATCA). The galaxies all lie near Galactic longitude 325 degrees and were selected because of their large angular sizes, up to 1.3 degrees. Linear sizes range from 53 to 108 kpc. The first galaxy is a massive 5.7x10^11 solar mass disk galaxy with a faint optical counterpart, SGC 1511.1--5249. The second is probably an interacting group of galaxies straddling the Galactic equator. No optical identification is possible. The third object appears to be an interacting pair of low column density galaxies, possibly belonging to an extended Circinus or Centaurus A galaxy group. No optical counterpart has been seen despite the predicted extinction (A(B) = 2.7 - 4.4 mag) not being excessive. We discuss the implications of the results, in particular the low HI column densities (~10^19 atoms/sq.cm) found for two of the three galaxies.Comment: 17 pages, 8 figures (Fig.1 in three parts, Fig.5 in two parts). To appear in Astronomical Journal (Dec 1998). See http://www.atnf.csiro.au/research/multibea

HI Bright Galaxies in the Southern Zone of Avoidance

A blind survey for HI bright galaxies in the southern Zone of Avoidance, (212 deg < l < 36 deg; |b| < 5 deg), has been made with the 21 cm multibeam receiver on the Parkes 64 m radiotelescope. The survey, sensitive to normal spiral galaxies to a distance of about 40 Mpc and more nearby dwarfs, detected 110 galaxies. Of these, 67 have no counterparts in the NASA/IPAC Extragalactic Database. In general, the uncataloged galaxies lie behind thicker obscuration than do the cataloged objects. All of the newly-discovered galaxies have HI flux integrals more than an order of magnitude lower than the Circinus galaxy. The survey recovers the Puppis cluster and foreground group (Kraan-Korteweg & Huchtmeier 1992), and the Local Void remains empty. The HI mass function derived for the sample is satisfactorily fit by a Schechter function with parameters alpha = 1.51 +- 0.12, Phi* = 0.006 +- 0.003, and log M* = 9.7 +- 0.10.Comment: To appear in The Astronomical Journa

Trace.space: a psychogeographical community project with members of an arts and health organisation

In this paper we theorise a situationist psychogeographical community group work project, conducted with members of an arts and health organization. Using creative ways to improve the mental health and well-being of individuals, we draw on the concepts of trace and spaces both to map relationships between researchers and group members and project implications. This project was driven by three aims which were: to do community group-work in order to produce contributions both inside and outside of the University; to use a psychogeographical approach to playfully critique everyday life in consumer capitalist society and finally, to consider the extent to which wider personal and political changes could be enabled. To realise these aims, we put into practice a range of architectural, critical community psychological and psychogeographical methods including photo-elicitation, dice walking and site specific investigations. We also facilitated participatory workshops via the creation of artistic and reflective maps and writing poems and stories. Reflections from all the stakeholders, conclusions and implications of this work are considered in terms of individual, group and societal changes. We argue for more psychogeographically inspired work

Combatting AMR : photoactivatable ruthenium(ii)-isoniazid complex exhibits rapid selective antimycobacterial activity

The novel photoactive ruthenium(II) complex cis-[Ru(bpy)2(INH)2][PF6]2 (1·2PF6, INH = isoniazid) was designed to incorporate the anti-tuberculosis drug, isoniazid, that could be released from the Ru(II) cage by photoactivation with visible light. In aqueous solution, 1 rapidly released two equivalents of isoniazid and formed the photoproduct cis-[Ru(bpy)2(H2O)2]2+ upon irradiation with 465 nm blue light. We screened for activity against bacteria containing the three major classes of cell envelope: Gram-positive Bacillus subtilis, Gram-negative Escherichia coli, and Mycobacterium smegmatis in vitro using blue and multi-colored LED multi-well arrays. Complex 1 is inactive in the dark, but when photoactivated is 5.5× more potent towards M. smegmatis compared to the clinical drug isoniazid alone. Complementary pump-probe spectroscopy measurements along with density functional theory calculations reveal that the mono-aqua product is formed in <500 ps, likely facilitated by a 3MC state. Importantly, complex 1 is highly selective in killing mycobacteria versus normal human cells, towards which it is relatively non-toxic. This work suggests that photoactivatable prodrugs such as 1 are potentially powerful new agents in combatting the global problem of antibiotic resistance

Heterogeneity within the Asian American community

BACKGROUND: Educational interventions are grounded on scientific data and assumptions about the community to be served. While the Pan Asian community is composed of multiple, ethnic subgroups, it is often treated as a single group for which one health promotion program will be applicable for all of its cultural subgroups. Compounding this stereotypical view of the Pan Asian community, there is sparse data about the cultural subgroups' similarities and dissimilarities. The Asian Grocery Store based cancer education program evaluation data provided an opportunity to compare data collected under identical circumstances from members of six Asian American cultural groups. METHODS: A convenience sample of 1,202 Asian American women evaluated the cultural alignment of a cancer education program, completing baseline and follow-up surveys that included questions about their breast cancer knowledge, attitudes, and screening behaviors. Participants took part in a brief education program that facilitated adherence to recommended screening guidelines. RESULTS: Unique recruitment methods were needed to attract participants from each ethnic group. Impressions gained from the aggregate data revealed different insights than the disaggregate data. Statistically significant variations existed among the subgroups' breast cancer knowledge, attitudes, and screening behaviors that could contribute to health disparities among the subgroups and within the aggregate Pan Asian community. CONCLUSION: Health promotion efforts of providers, educators, and policy makers can be enhanced if cultural differences are identified and taken into account when developing strategies to reduce health disparities and promote health equity

The disruption of nearby galaxies by the Milky Way

Interactions between galaxies are common and are an important factor in determining their physical properties such as position along the Hubble sequence and star-formation rate. There are many possible galaxy interaction mechanisms, including merging, ram-pressure stripping, gas compression, gravitational interaction and cluster tides. The relative importance of these mechanisms is often not clear, as their strength depends on poorly known parameters such as the density, extent and nature of the massive dark halos that surround galaxies. A nearby example of a galaxy interaction where the mechanism is controversial is that between our own Galaxy and two of its neighbours -- the Large and Small Magellanic Clouds. Here we present the first results of a new HI survey which provides a spectacular view of this interaction. In addition to the previously known Magellanic Stream, which trails 100 degrees behind the Clouds, the new data reveal a counter-stream which lies in the opposite direction and leads the motion of the Clouds. This result supports the gravitational model in which leading and trailing streams are tidally torn from the body of the Magellanic Clouds.Comment: 17 pages with 5 figures in gif format, scheduled for publication in the August 20th, 1998 issue of Natur

The Fornax Spectroscopic Survey: The Number of Unresolved Compact Galaxies

We describe a sample of thirteen bright (18.5<Bj<20.1) compact galaxies at low redshift (0.05<z<0.21) behind the Fornax Cluster. These galaxies are unresolved on UK Schmidt sky survey plates, so would be missing from most galaxy catalogs compiled from this material. The objects were found during initial observations of The Fornax Spectroscopic Survey. This project is using the Two-degree Field spectrograph on the Anglo-Australian Telescope to obtain spectra for a complete sample of all 14000 objects, stellar and non-stellar, with 16.5<Bj<19.7, in a 12 square degree area centered on the Fornax cluster of galaxies. The surface density of compact galaxies with magnitudes 16.5<Bj<19.7 is 7+/-3 /sq.deg., representing 2.8+/-1.6% of all local (z<0.2) galaxies to this limit. There are 12+/-3 /sq.deg. with 16.5<Bj<20.2. They are luminous (-21.5<Mb<-18.0, for H0=50 km/s/mpc) and most have strong emission lines (H alpha equivalent widths of 40-200 A) and small sizes typical of luminous HII galaxies and compact narrow emission line galaxies. Four out of thirteen have red colors and early-type spectra, so are unlikely to have been detected in any previous surveys.Comment: LaTeX source; 5 pages including 3 figures; uses emulateapj.st