Luminescence study of dolomite mineral

Not availabl

siRNA knockdown of SPHK1 in vivo protects mice from systemic, type-I Allergy.

Systemic anaphylaxis is considered to be a typical immediate hypersensitivity response, determined by the activation of immune cells,
via antigen-induced aggregation of IgE-sensitized FcεRI cells. Perhaps most the important cells, in the immediate hypersensitivity responses, are mast cells. We have previously shown that SPHK1 plays a key role in the intracellular signaling pathways triggered by FceRI aggregation on human
mast cells. More recently, we performed a genome-wide gene expression profiling of human mast cells, sensitized with IgE alone, or stimulated by FcεRI aggregation. We found that sphingosine kinase 1 (SPHK1) was one
of genes activated at the earlier stages of mast cell activation, including during sensitization. Moreover, SPHK1 has been shown, by us and others, to be a key player in the intracellular signaling pathways triggered by
several immune-receptors, including fMLP, C5a, and Fcg- and Fcereceptors. Here we have investigated the in vivo role of SPHK1 in allergy, using a specific siRNA to knockdown SPHK1 in vivo. Our results support a role for
SPHK1 in the inflammatory responses that share clinical, immunological, and histological features of type I hypersensitivity. Thus, mice pretreated with the siRNA for SPHK1 were protected from the IgE mediated allergic
reactions including: temperature changes, histamine release, cytokine production, cell-adhesion molecule expression, and immune cell infiltration into the lungs

സംരംഭകത്വ വികസന പരിപാടി : കാലാവസ്ഥാ അതിജീവന മത്സ്യകൃഷി - 2015

സി.എം.എഫ്.ആർ.ഐ - നിക്ര പ്രസിദ്ധീകരണം : 4 സംരംഭകത്വ വികസന പരിപാടി : കാലാവസ്ഥാ അതിജീവന മത്സ്യകൃഷി - 201

Innovative programme for creating entrepreneurs: Success stories in sea food micro enterprises

Krishi Vigyan Kendra (Ernakulam) formulated a special training programme with an intention to create micro -sea food entrepreneurs. There was no stipulated time for the training programme, instead it continued till the selected entrepreneurs become successful. Follow up services are also being given to the successful entrepreneurs. Selection of the trainees was based on their accessibility to the raw material and availability of space for processing, packing and forwarding. The programme covered all aspects of initiating a sea food enterprise. The syllabus covered selection of good quality raw fish at a cheaper price from the auctioning centers near Kochi. The first lesson was to learn the process of fish auctioning. Subsequently, the candidates were trained in the transportation of bulk quantity of fish without quality degradation followed by temporary storage and scientific cutting and cleaning. Selection of quality additives like spices, oil etc. in bulk quantities in cheaper price and their storage were covered. Introduction of processing gadgets, labor & time management, product formulation, packing, attractive label designing, designing of trademark, registration of trademark, the importance of branding etc. were covered. In addition, the statutory licenses and certificates required for the industry and step by step procedure to obtain the same were also included in the syllabus. Tie-ups were made with retail marketing outlets and test marketing of the products were done by the participating trainees. Methods in finding out the marketing channels and waste utilization were also covered. Two entrepreneurs started products under their own brand name. The products are fish pickle, squid pickle, dry prawn roast, prawn chutney powder and ready to cook dry prawn. One entrepreneur started exporting dry prawn products and prawn wafers

കർഷക പങ്കാളിത്ത സംരംഭങ്ങൾ

എറണാകുളം ജില്ലാ കൃഷി വിഞ്ജാന കേന്ദ്രം കർഷകരുമായി സഹകരിച്ചുകൊണ്ട് ശാസ്ത്രീയ രീതിയിലുള്ള കരിമീൻ വിത്തുൽപ്പാദനം, മുയൽ കുഞ്ഞുങ്ങളുടെ ഉത്പാദനം എന്നിവ വ്യാവസായിക അടി സ്ഥാനത്തിൽ നടത്തുവാൻ തീരുമാനിച്ചിരിക്കുന്നു. മേൽ പറഞ്ഞവയുടെ വിപണനം കെ.വി.കെ. വഴി നടത്തുന്നതുകൊണ്ട് പങ്കാളിയായ സംരംഭകർക്ക് ലാഭകരമായ സംരംഭമായും കർഷകർക്ക് ഗുണമേന്മയുള്ള കുഞ്ഞുങ്ങളെ ലഭ്യമാക്കുന്നതിനുള്ള അവസരമായും ഈ പദ്ധതി വർത്തിക്കുന്നു. ഈ പദ്ധതിയിൽ താൽപര്യമുള്ള കർഷകൻ അപേക്ഷ സമർപ്പിക്കുന്നതിനനുസരിച്ച് കെവികെയിലെ സാങ്കേതിക വിദഗ്‌ദർ സ്ഥലം സന്ദർശിച്ച് സാഹ്യചര്യങ്ങളും സ്ഥലസൗകര്യങ്ങളും അനുയോജ്യമാണോ എന്ന് ഉറപ്പാക്കുന്നതാണ്. തുടർന്ന് അനുയോജ്യരായ കർഷകർക്ക് സാധ്യതാ പഠന റിപ്പോർട്ടും, സാങ്കേതികാ വിവര റിപ്പോർട്ടും, എസ്റ്റിമേറ്റും തയ്യാറാക്കി നൽകുന്നതാണ്. മേൽ പറഞ്ഞ റിപ്പോർട്ടുകൾ പ്രകാരം സൗകര്യങ്ങൾ ഒരുക്കുന്ന കർഷകരുമായി ഒരു സംയുക്ത ഉടമ്പടിയിൽ ഏർപ്പെടുന്നതും ഇവരെ കെ.വി.കെ.യുടെ വിത്തുൽപാദന പങ്കാളിയായി അംഗീകരിക്കുന്നതുമാണ്. ഇത്തരം യൂണിറ്റുകളെ കെ.വി.കെ.യുടെ ഉപഗ്രഹ വിത്തുല്‌പാദന കേന്ദ്രങ്ങൾ എന്നായിരിക്കും നാമകരണം ചെയ്യുക

Mulching brown seaweed Sargassum wightii during transplant on the growth and yield of paddy

Seaweeds gain importance recently in agriculture as several studies have shown that chemicals extracted from seaweeds are used as soil amendments to increase crop yield. We report here the positive effects of brown seaweed Sargassum wightii on paddy when applied as mulch during transplantation trials carried out in a progressive farmer’s field

KVK Ernakulam Newsletter (malayalam) April 2014 to Sept, 2014

KVK Ernakulam Newsletter (malayalam) April 2014 to Sept, 2014KVK Ernakulam Newsletter (malayalam) April 2014 to Sept, 2014Not Availabl

Decade of Progress in Technology Dissemination: Illustrated through Newspaper Clippings

ICAR-Krishi Vigyan Kendra (Ernakulam) CMFRI: A Decade of Progress in Technology Dissemination: Illustrated through Newspaper Clipping

In Silico Sperm Proteome Analysis to Investigate DNA Repair Mechanisms in Varicocele Patients

This study was supported by the American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, Ohio, USA.Varicocele, a condition associated with increased oxidative stress, negatively affects sperm DNA integrity and reduces pregnancy rates. However, the molecular mechanisms related to DNA integrity, damage, and repair in varicocele patients remain unclear. This study aimed to determine the role of DNA repair molecular mechanisms in varicocele-related infertility by combining an in silico proteomics approach with wet-laboratory techniques. Proteomics results previously generated from varicocele patients (n=50) and fertile controls (n=10) attending our Andrology Center were reanalyzed using bioinformatics tools, including the WEB-based Gene SeT AnaLysis Toolkit, Open Target Platform, and Ingenuity Pathway Analysis (IPA), to identify differentially expressed proteins (DEPs) involved in DNA repair. Subsequently, selected DEPs in spermatozoa were validated using western blotting in varicocele (n = 13) and fertile control (n = 5) samples. We identified 99 DEPs mainly involved in male reproductive system disease (n=66) and male infertility (n=47). IPA analysis identified five proteins [fatty acid synthase (FASN), myeloperoxidase (MPO), mitochondrial aconitate hydratase (ACO2), nucleoporin 93 (NUP93), and 26S proteasome non-ATPase regulatory subunit 14 (PSMD14)] associated with DNA repair deficiency, which showed altered expression in varicocele (P <0.03). We validated ACO2 downregulation (fold change=0.37, change%=-62.7%, P=0.0001) and FASN overexpression (fold change = 4.04, change %= 303.7%, P = 0.014) in men with varicocele compared to controls. This study combined a unique in silico approach with an in vitro validation of the molecular mechanisms that may be responsible for varicocele-associated infertility. We identified ACO2 and FASN as possible proteins involved in DNA repair, whose altered expression may contribute to DNA damage in varicocele pathophysiology. Copyright © 2021American Center for Reproductive Medicine, Cleveland Clini