1,685 research outputs found
The HLA-E Gene encodes two differentially regulated Transcripts and a Cell Surface Protein
An HLA-E-specific oligonucleotide probe was used
to study the expressioonf HLA-E. This probed etects
two HLA-E transcripts, 1.8 and 2.7 kb in size, which
are present in varying ratios in allt issues and cell
lines investigated. We demonstrate that alternative
poly(A) site usage accounts for the differential regulation
of the two HLA-E mRNA species. Sequence
analysis of three cDNA clones, representing the two
transcripts of HLA-E, and of anH LA-E gene encoded
by cosmid cd3.14, revealed identity of gene and
cDNA in the 3’ untranslated region. S1 nuclease
protection assays confirmed that the two HLA-E
transcripts are not alternative splicing products.
Introduction of cd3.14, together with human ,&m
into the murine myeloma cell line P3X63-Ag8.653,
resulted in a cell surface expresosf ioan HLA-class
I heavy chain detectablbey indirect immunofluorescence
whereas transfection into the humBaznr n expressing
mouse L cell line, 527 was negative with
regard to cell surface expressionC. ell surface labeling
of transfectants and immunoprecipitation with
a monomorphic HLA class I-specific antibodyo r an
antibody against human &m confirmed the presence
of an HLA-E H chain on the cell surface. These
results indicate that the HLA-E gene codes for a
class I H chain that can be expressed on the cell
surface
Utilization of a fixed base simulator to study the stall and spin characteristics of fighter airplanes
Feasibility of using fixed simulator to determine stall and spin characteristics of fighter aircraf
Spin vectors in the Koronis family: V. Resolving the ambiguous rotation period of (3032) Evans
A sidereal rotation counting approach is demonstrated by resolving an
ambiguity in the synodic rotation period of Koronis family member (3032) Evans,
whose rotation lightcurves' features did not easily distinguish between doubly-
and quadruply-periodic. It confirms that Evans's spin rate does not exceed the
rubble-pile spin barrier and thus presents no inconsistency with being a ~14-km
reaccumulated object. The full spin vector solution for Evans is comparable to
those for the known prograde low-obliquity comparably-fast rotators in the
Koronis family, consistent with having been spun up by YORP thermal radiation
torques.Comment: 8 pages, 6 figures, accepted for publication in Icaru
Charon's radius and density from the combined data sets of the 2005 July 11 occultation
The 2005 July 11 C313.2 stellar occultation by Charon was observed by three
separate research groups, including our own, at observatories throughout South
America. Here, the published timings from the three data sets have been
combined to more accurately determine the mean radius of Charon: 606.0 +/- 1.5
km. Our analysis indicates that a slight oblateness in the body (0.006 +/-
0.003) best matches the data, with a confidence level of 86%. The oblateness
has a pole position angle of 71.4 deg +/- 10.4 deg and is consistent with
Charon's pole position angle of 67 deg. Charon's mean radius corresponds to a
bulk density of 1.63 +/- 0.07 g/cm3, which is significantly less than Pluto's
(1.92 +/- 0.12 g/cm3). This density differential favors an impact formation
scenario for the system in which at least one of the impactors was
differentiated. Finally, unexplained differences between chord timings measured
at Cerro Pachon and the rest of the data set could be indicative of a
depression as deep as 7 km on Charon's limb.Comment: 25 pages including 4 tables and 2 figures. Submitted to the
Astronomical Journal on 2006 Feb 0
Operational streamlining in a high-throughput genome sequencing center
Thesis (M.B.A.)--Massachusetts Institute of Technology, Sloan School of Management; and, (S.M.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering; in conjunction with the Leaders for Manufacturing Program at MIT, 2006.Includes bibliographical references (p. 83-84).Advances in medicine rely on accurate data that is rapidly provided. It is therefore critical for the Genome Sequencing platform of the Broad Institute of MIT and Harvard to continually strive to reduce cost, improve throughput, and increase the quality of its data output. In the past, new technology in the form of both chemistry improvements and robotics has allowed the Institute to achieve these goals in a step-wise manner. However, as the rate of technology progression in sequencing has slowed, the Institute has been forced to look to continuous, incremental improvement in order to achieve its goals. The Core Sequencing/Detection group handles the high-throughput sequencing duties at the Broad Institute. Through the use of robotics and cutting edge biology, they are able to process and sequence upwards of 50 billion bases of DNA per year. The work that this thesis was based on took place primarily in this automated production area. This thesis utilizes a number of lean concepts, including the 7 Wastes and pull production control.(cont.) Kanban systems, workflow changes, and a 5S implementation were used to bring these concepts to life at the Broad Institute. In order to correctly size the kanban system, process buildup diagrams and discrete event simulation were used. Each of these tools helped to drive the process towards the Institute's goals of reducing cost and improving quality and throughput.by Kerry P. Person.S.M.M.B.A
Long-term outcome of noninvasive positive pressure ventilation for obesity hypoventilation syndrome
BACKGROUND: Few data are available on the long-term outcome of noninvasive positive pressure ventilation (NPPV) for obesity hypoventilation syndrome (OHS). This study was designed to determine long-term survival, treatment adherence, and prognostic factors in patients with OHS in whom NPPV was initiated in an acute setting vs under stable clinical conditions.METHODS: One hundred thirty consecutive patients with OHS (56 women) who started NPPV between January 1995 and December 2006 either under stable conditions (stable group, n = 92) or during ICU management of acute hypercapnic exacerbation (acute group, n = 38) were retrospectively analyzed. RESULTS: Arterial blood gases and the Epworth sleepiness scale were both significantly improved after 6 months of NPPV. With a mean follow-up of 4.1 +/- 2.9 years, 24 (18.5%) patients died and 24 (18.5%) discontinued NPPV. On Kaplan-Meier analysis, 1-, 2-, 3-, and 5-year survival probabilities were 97.5%, 93%, 88.3%, and 77.3%, respectively. Mortality was lower than that described in a previous series of patients with untreated OHS. Supplemental oxygen therapy was the only independent predictor of mortality. The probability of continuing NPPV was 80% at 3 years with a high rate of daily use ( > 7 h). Female sex was predictive of lower long-term adherence to NPPV. The acute and stable groups did not differ in terms of arterial blood gases and Epworth sleepiness scale at 6 months, long-term survival, and treatment adherence. CONCLUSIONS: The results of this study support long-term NPPV as an effective and well-tolerated treatment of OHS whether initiated in the acute or chronic setting
Pneumopathie médicamenteuse sous traitement par venlafaxine et propranolol
Introduction
Venlafaxine and propranolol have rarely been identified as causes of pulmonary pathology. We describe a case of drug-induced pneumonitis occurring in a patient treated with these two medications.
Case report
A 55 years old woman with liver cirrhosis treated with venlafaxine for 1 year and propranolol for 1 month was admitted to the intensive care unit because of acute respiratory failure. A Mycoplasma pneumoniae pneumonitis was diagnosed. After initial improvement under antibiotics, a new deterioration of respiratory status was observed 4 days after the reintroduction of venlafaxine and propranolol. Spontaneous recovery occurred after these treatments were withheld. Co administration of venlafaxine and propranolol, 2 drugs with affinity for the same cytochrome P450 isoenzyme (CYP2D6), may have contributed to drug accumulation and pulmonary toxicity. The liver cirrhosis of our patient may also have contributed to decreased cytochrome P450 enzymatic activity.
Conclusions
Venlafaxine and propranolol share the same metabolic pathway and their co-administration may be complicated by drug induced pneumonitis
Dipole Antenna Printed on Paper Substrate for WLAN Applications
International audienceThe design of a dipole antenna printed on a paper substrate is presented in this paper. The antenna which integrates a compact balun is devoted for dual-band 2.4/5 GHz WLAN applications. The antenna is based on a double-side printed multilayer paper substrate and is fed with a coaxial cable for the testing. The simulated results of the whole structure are also presented in detail and compared with the measured performances
The Mission Accessible Near-Earth Objects Survey: Four years of photometry
Over 4.5 years, the Mission Accessible Near-Earth Object Survey (MANOS)
assembled 228 Near-Earth Object (NEO) lightcurves. We report rotational
lightcurves for 82 NEOs, constraints on amplitudes and periods for 21 NEOs,
lightcurves with no detected variability within the image signal to noise and
length of our observing block for 30 NEOs, and 10 tumblers. We uncovered 2
ultra-rapid rotators with periods below 20s; 2016MA with a potential rotational
periodicity of 18.4s, and 2017QG rotating in 11.9s, and estimate the
fraction of fast/ultra-rapid rotators undetected in our project plus the
percentage of NEOs with a moderate/long periodicity undetectable during our
typical observing blocks. We summarize the findings of a simple model of
synthetic NEOs to infer the object morphologies distribution using the measured
distribution of lightcurve amplitudes. This model suggests a uniform
distribution of axis ratio can reproduce the observed sample. This suggests
that the quantity of spherical NEOs (e.g., Bennu) is almost equivalent to the
quantity of highly elongated objects (e.g., Itokawa), a result that can be
directly tested thanks to shape models from Doppler delay radar imaging
analysis. Finally, we fully characterized 2 NEOs as appropriate targets for a
potential robotic/human mission: 2013YS and 2014FA due to their
moderate spin periods and low .Comment: Accepted for Publication, The Astrophysical Journal Supplement Serie
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