Finite Sample Properties of Moran's I Test for Spatial Autocorrelation in Probit and Tobit Models - Empirical Evidence

In this paper, we investigate the finite sample properties of Moran’s I test statistic for spatial autocorrelation in limited dependent variable models suggested by Kelejian and Prucha (2001). We analyze the socio- economic determinants of the availability of dialysis equipment in 5,507 Brazilian municipalities in 2009 by means of a probit and tobit specifica- tion. We assess the extent to which evidence of spatial autocorrelation can be remedied by the inclusion of spatial fixed effects. We find spa- tial autocorrelation in both model specifications. For the probit model, a spatial fixed effects approach removes evidence of spatial autocorrelation. However, this is not the case for the tobit specification. We further fill a void in the theoretical literature by investigating the finite sample prop- erties of these test statistics in a series of Monte Carlo simulations, using data sets ranging from 49 to 15,625 observations. We find that the tests are unbiased and have considerable power for even medium-sized sample sizes. Under the null hypothesis of no spatial autocorrelation, their em- pirical distribution cannot be distinguished from the asymptotic normal distribution, empirically confirming the theoretical results of Kelejian and Prucha (2001), although the sample size required to achieve this result is larger in the tobit case than in the probit case.

Thermofield-Bosonization on Compact Space

We develop the construction of fermionic fields in terms of bosonic ones to describe free and interaction models in the circle, using thermofielddynamics. The description in the case of finite temperature is developed for both normal modes and zero modes. The treatment extends the thermofield-bosonization for periodic space

Workplace Romance and Fraternization Policies

With the ever increasing number of hours Americans spend at work, many are finding romance at the workplace. What should the employer consider when deciding whether and to what extent it should control romantic relationships between supervisors and subordinates and among co-workers? This paper addresses some of the social and legal issues surrounding these relationships and whether fraternization policies are a viable tool for handling the complex human issue of romance in the workplace

Transcriptional regulation in skeletal muscle of zebrafish in response to nutritional status, photoperiod and experimental selection for body size

In the present study, the ease of rearing, short generation time and molecular research tools available for the zebrafish model (Danio rerio, Hamilton) were exploited to investigate transcriptional regulation in relation to feeding, photoperiod and experimental selection. Chapter 2 describes transcriptional regulation in fast skeletal muscle following fasting and a single satiating meal of bloodworms. Changes in transcript abundance were investigated in relation to the food content in the gut. Using qPCR, the transcription patterns of 16 genes comprising the insulin-like growth factor (IGF) system were characterized, and differential regulation between some of the paralogues was recorded. For example, feeding was associated with upregulation of igf1a and igf2b at 3 and 6h after the single-meal was offered, respectively, whereas igf1b was not detected in skeletal muscle. On the other hand, fasting triggered the upregulation of the igf1 receptors and igfbp1a/b, the only binding proteins whose transcription was responsive to a single-satiating meal. In addition to the investigation of the IGF-axis, an agnostic approach was used to discover other genes involved in transcriptional response to nutritional status, by employing a whole-genome microarray containing 44K probes. This resulted in the discovery of 147 genes in skeletal muscle that were differentially expressed between fasting and satiation. Ubiquitin-ligases involved in proteasome-mediated protein degradation, and antiproliferative and pro-apoptotic genes were among the genes upregulated during fasting, whereas satiation resulted in an upregulation of genes involved in protein synthesis and folding, and a gene highly correlated with growth in mice and fish, the enzyme ornithine decarboxylase 1. Zebrafish exhibit circadian rhythms of breeding, locomotor activity and feeding that are controlled by molecular clock mechanisms in central and peripheral organs. In chapter 3 the transcription of 17 known clock genes was investigated in skeletal muscle in relation to the photoperiod and food content in the gut. The hypothesis that myogenic regulatory factors and components of the IGF-pathway were clock-controlled was also tested. Positive (clock1 and bmal1 paralogues) and negative oscillators (cry1a and per genes) showed a strong circadian pattern in skeletal muscle in anti-phase with each other. MyoD was not clock-controlled in zebrafish in contrast to findings in mice, whereas myf6 showed a circadian pattern of expression in phase with clock and bmal. Similarly, the expression of two IGF binding proteins (igfbp3 and 5b) was circadian and in phase with the positive oscillators clock and bmal. It was also found that some paralogues responded differently to photoperiod. For example, clock1a was 3-fold more responsive than clock1b. Cry1b did not show a circadian pattern of expression. These patterns of expression provide evidence that the molecular clock mechanisms in skeletal muscle are synchronized with the molecular clock in central pacemaker organs such as eyes and the pineal gland. Using the short generation time of zebrafish the effects of selective breeding for body size at age were investigated and are described in chapter 4. Three rounds of artificial selection for small (S-lineage) and large body size (L-lineage) resulted in zebrafish populations whose average standard length were, respectively, 2% lower and 10% higher than an unselected control lineage (U-lineage). Fish from the L-lineage showed an increased egg production and bigger egg size with more yolk, possibly contributing to the larger body size observed in the early larval stage (6dpf) of fish from this lineage. Fish from S- and L-lineage exposed to fasting and refeeding showed very similar feed intake, providing evidence that experimental selection did not cause significant changes in appetite control. Investigation of the expression of the IGF-axis and nutritionally-response in skeletal muscle after fasting and refeeding revealed that the pattern of expression was not different between the selected lineages, but that a differential responsiveness was observed in a limited number of genes, providing evidence that experimental selection might have changed the way fish allocate the energy acquired through feeding. For example, a constitutive higher expression of igf1a was recorded in skeletal muscle of fish from the L-lineage whereas igfbp1a/b transcripts were higher in muscle of fish from the S-lineage. These findings demonstrate the rapid changes in growth and transcriptional response in skeletal muscle of zebrafish after only three rounds of selection. Furthermore, it provides evidences that differences in growth during embryonic and larval stages might be related to higher levels of energy deposited during oogenesis, whereas differences in adult fish were better explained by changes in energy allocation instead of energy acquisition. In chapter 5 the main findings made during this study and their impact on the literature are discussed.European Community's Seventh Framework Programme (FP7/2007-2013) under grant agreement n° 222719 – LIFECYCLEFunded by the Seventh Framework Programme (European Commission); Programme Alβan, the European Union Programme of High Level Scholarships for Latin America (scholarship n° E07D402823BR); the Capes Foundation, Ministry of Education of Brazil (scholarship nº BEX 0449-10-5); and the Sir Harold Mitchell Fun