Uklanjanje obostranog dermoida na bulbarnoj spojnici šteneta pasmine labrador retriver elektrokauterom - prikaz slučaja.

A Labrador retriever pup with a bilateral dermoid in the eyes was treated successfully by the use of a unipolar hand held electrocautery unit.Štene pasmine labrador retriver s obostranim dermoidom u očima bilo je uspješno izliječeno upotrebom jednostranog ručnog elektrokautera

Differentiation and displacement: Unpicking the relationship between accounts of illness and social structure

This article seeks to unpack the relationship between social structure and accounts of illness. Taking dentine hypersensitivity as an example, this article explores the perspective that accounts of illness are sense-making processes that draw on a readily available pool of meaning. This pool of meaning is composed of a series of distinctions that make available a range of different lines of communication and action about such conditions. Such lines of communication are condensed and preserved over time and are often formed around a concept and its counter concept. The study of such processes is referred to as semantic analysis and involves drawing on the tools and techniques of conceptual history. This article goes on to explore how the semantics of dentine hypersensitivity developed. It illustrates how processes of social differentiation led to the concept being separated from the more dominant concept of dentine sensitivity and how it was medicalised, scientised and economised. In short, this study seeks to present the story of how society has developed a specific language for communicating about sensitivity and hypersensitivity in teeth. In doing so, it proposes that accounts of dentine hypersensitivity draw on lines of communication that society has preserved over time

Performance evaluation and sensitivity analysis of ASTER and SRTM (30M) DEM-derived terrain variables in landslide susceptibility assessment: A case from the western ghats

© Kaunas University of Technology. Performance and sensitivity of freely available equal resolution space-borne digital elevation model derivatives in landslide susceptibility analysis were carried out in a selected part of the Western Ghats, India. ASTER and SRTM digital elevation models having a 30-m resolution were used to derive the terrain variables such as slope, aspect, relative relief, slope length and steepness, curvature, landform and stream networks. Most of the variables showed spatial variability in distribution pattern, which affects the results of geo-environmental processes analysed. Sensitivity and performance of each variable derived from the digital elevation models were assessed by preparing landslide susceptibility index (LSI) maps using the Information Value (InfoVal) technique and were validated through receiver operator characteristics (ROC) curve analysis. LSI maps generated point towards the capability of the SRTM digital elevation model to correctly generate the terrain variables than the ASTER elevation surface, by giving the accuracy of LSI maps greater than those produced using the ASTER-derived parameters (0.77 and 0.72 for SRTM; 0.67 and 0.65 for ASTER). The results of the present study suggest that the SRTM digital elevation data are more sensitive and suitable for terrain analysis and earth surface process modelling than the ASTER elevation data sets, although both possess equal resolutions

Mesenchymal stem cells-seeded bio-ceramic construct for bone regeneration in large critical-size bone defect in rabbit

Bone marrow derived mesenchymal stem cells (BMSC) represent an attractive cell population for tissue engineering purpose. The objective of this study was to determine whether the addition of recombinant human bone morphogenetic protein (rhBMP-2) and insulin-like growth factor (IGF-1) to a silica-coated calcium hydroxyapatite (HASi) - rabbit bone marrow derived mesenchymal stem cell (rBMSC) construct promoted bone healing in a large segmental bone defect beyond standard critical -size radial defects (15mm) in rabbits. An extensively large 30mm long radial ostectomy was performed unilaterally in thirty rabbits divided equally in five groups. Defects were filled with a HASi scaffold only (group B); HASi scaffold seeded with rBMSC (group C); HASi scaffold seeded with rBMSC along with rhBMP-2 and IGF-1 in groups D and E respectively. The same number of rBMSC (five million cells) and concentration of growth factors rhBMP-2 (50µg) and IGF-1 (50µg) was again injected at the site of bone defect after 15 days of surgery in their respective groups. An empty defect served as the control group (group A). Radiographically, bone healing was evaluated at 7, 15, 30, 45, 60 and 90 days post implantation. Histological qualitative analysis with microCT (µ-CT), haematoxylin and eosin (H & E) and Masson’s trichrome staining were performed 90 days after implantation. All rhBMP-2-added constructs induced the formation of well-differentiated mineralized woven bone surrounding the HASi scaffolds and bridging bone/implant interfaces as early as eight weeks after surgery. Bone regeneration appeared to develop earlier with the rhBMP-2 constructs than with the IGF-1 added construct. Constructs without any rhBMP-2 or IGF-1 showed osteoconductive properties limited to the bone junctions without bone ingrowths within the implantation site. In conclusion, the addition of rhBMP-2 to a HASi scaffold could promote bone generation in a large critical-size-defect

Mesenchymal stem cells-seeded bio-ceramic construct for bone regeneration in large critical-size bone defect in rabbit

Bone marrow derived mesenchymal stem cells (BMSC) represent an attractive cell population for tissue engineering purpose. The objective of this study was to determine whether the addition of recombinant human bone morphogenetic protein (rliBMP-2) and insulin-like growth factor (IGF-1) to a silica-coated calcium hydroxyapatite (HASi)- rabbit bone marrow derived mesenchymal stem cell (rBlVISC) construct promoted bone healing in a large segmental bone defect beyond standard critical-size radial defects (15mm) in rabbits. An extensively large 30111111 long radial ostectomy was performed unilaterally in thirty rabbits divided equally in five groups. Defects were filled with a HASi scaffold only (group B), HASi scaffold seeded with rl3VISC (group C); HASi scaffold seeded with 113NISC along with rhl3MP-2 and IGF-1 in groups D and E respectively. The same number of rBMSC (five million cells) and concentration of growth factors rhBMP-2 (50ps) and IGF-1 (50g) was again injected at the site of bone defect after 15 days of surgery in their respective groups. An empty defect served as the control group (group A). Radiographically, bone healing was evaluated at 7, 15, 30, 45, 60 and 90 days post implantation. Histological qualitative analysis with microCT (pt-CT), haematoxylin and eosin (H & E) and Masson's trichrome staining were performed 90 days after implantation. All rhBMP-2-ackled constructs induced the formation of well-differentiated mineralized woven bone surrounding the HASi scaffolds and bridging bone/implant interfaces as early as eight weeks after surgery. Bone regeneration appeared to develop earlier with the rliBIAP-2 constructs than with the IGF-1 added construct. Constructs without any rItEMP-2 or IGF-1 showed osteoconductive properties limited to the bone junctions without bone ingrowths within the implantation site. In conclusion, the addition of rhBIVIP-2 to a HASi scaffold could promote bone generation large critical-size-defect

Isolation, proliferation, characterization and <i>in vivo</i> osteogenic potential of bone-marrow derived mesenchymal stem cells (rBMSC) in rabbit model

79-87Information on isolation, characterization of rabbit MSC and its evaluation in critical bone defect (CSD) is scarcely available. Here, we attempted to isolate, proliferate, differentiate, characterize and evaluate the in vivo osteogenic potential of bone marrow derived mesenchymal stem cells (BMSCs) collected from New Zealand White rabbits. They were isolated and proliferated in antibiotic supplemented DMEM (Dulbecco’s Modified Eagle’s media). Osteogenic differentiation of rabbit bone marrow derived mesenchymal stem cells (rBMSCs) was induced by osteogenic supplements and evaluated by alizarin red staining and alkaline phosphatase activity assay and characterized by specific CD surface antigen markers through FACS (Fluorescent activated cell shorting) and RT-PCR. Day ‘0’ cells were round/oval and floating, and on day 3-5, cell attachment with spindle/polygonal/star morphology was seen. On subsequent passages, they assumed uniform spindle shaped morphology. After culturing in respective differentiation media rBMSCs showed increased alkaline phosphatase activity, intense alizarin red staining, blue staining for Alcian blue and deep red colour on oil red O staining supporting the osteogenic, chondrogenic and adipogenic differentiation ability. In vivo osteogenic potential of rBMSCs was evaluated in a 30 mm critical sized defect of rabbit radius. The cellular morphology of plastic adherent cells was seen as single cell form in P0 and in P1, P2 and P3, as elongated/spindle-shape in clusters. The rBMSCs were positive for CD44, CD73 and CD105 and negative for CD34 and CD45 and could differentiate to osteogenic cells in osteogenic induction media. The in vivo experiments in rabbit CSD model confirmed that rBMSCs promote faster healing of critical size defects. Hence, we may suggest that rBMSCs are suitable for bone formation in fracture healing and non-union

Nitrate and nitrite exposure in Italian populations with different gastric cancer rates.

Exposure to nitrate and nitrite from dietary sources was estimated by questionnaire and measurement of salivary levels of the ions in residents of four regions of Italy with longstanding, contrasting, gastric cancer mortality rates. Whether using salivary levels or dietary questionnaire estimations no association was found between nitrate and nitrite exposure and gastric cancer mortality rates. For salivary nitrate and nitrite, the intra-provincial variation was greater than any inter-province difference. However, for dietary nitrate and nitrite intakes, there were significant differences between the regional groups (but not related to gastric cancer risk). Despite the limitations of the methods used, there was a weak positive association between salivary concentrations of nitrate and questionnaire assessment. Dietary factors are likely to play key roles at different stages of the gastric carcinogenic process. Nitrate may play a part but is unlikely to be a rate-limiting factor in all individuals or populations