29 research outputs found
Glycosylation site prediction using ensembles of Support Vector Machine classifiers
Article discussing the performance of different computational methods for prediction of glycosylation sites from amino acid sequences
T7 RNA Polymerase Functions In Vitro without Clustering
Many nucleic acid polymerases function in clusters known as factories. We investigate whether the RNA polymerase (RNAP) of phage T7 also clusters when active. Using ‘pulldowns’ and fluorescence correlation spectroscopy we find that elongation complexes do not interact in vitro with a Kd<1 µM. Chromosome conformation capture also reveals that genes located 100 kb apart on the E. coli chromosome do not associate more frequently when transcribed by T7 RNAP. We conclude that if clustering does occur in vivo, it must be driven by weak interactions, or mediated by a phage-encoded protein
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Piezoelectric motor development at AlliedSignal Inc., Kansas City Division
The Kansas City Division of AlliedSignal Inc. has been investigating the fabrication and use of piezoelectric motors in mechanisms for United States Department of Energy (DOE) weapons applications for about four years. These motors exhibit advantages over solenoids and other electromagnetic actuators. Prototype processes have been developed for complete fabrication of motors from stock materials, including abrasive machining of piezoelectric ceramics and more traditional machining of other motor components, electrode plating and sputtering, electric poling, cleaning, bonding and assembly. Drive circuits have been fabricated and motor controls are being developed. Laboratory facilities have been established for electrical/mechanical testing and evaluation of piezo materials and completed motors. Recent project efforts have focused on the potential of piezoelectric devices for commercial and industrial use. A broad range of various motor types and application areas has been identified, primarily in Japan. The Japanese have been developing piezo motors for many years and have more recently begun commercialization. Piezoelectric motor and actuator technology is emerging in the United States and quickly gaining in commercial interest. The Kansas City Division is continuing development of piezoelectric motors and actuators for defense applications while supporting and participating in the commercialization of piezoelectric devices with private industry through various technology transfer and cooperative development initiatives
Austral birds offer insightful complementary models in ecology and evolution
Theuerkauf J, Villavicencio CP, Adreani NM, et al. Austral birds offer insightful complementary models in ecology and evolution. Trends in Ecology & Evolution. 2022;37(9):759-767.The Southern Hemisphere differs in many aspects from the Northern Hemisphere. However, most ecological and evolutionary research is conducted in the Northern Hemisphere and its conclusions are extrapolated to the entire globe. Therefore, unique organismal and evolutionary characteristics of the south are overlooked. We use ornithology to show the importance of including a southern perspective. We present examples of plumage pigmentation, brood-parasitic nestling ejection, flightlessness, female song, and female aggression modulated by progesterone as complementary models for investigating fundamental biological questions. More research in the Southern Hemisphere, together with increased cooperation among researchers across the hemispheres and within the Southern Hemisphere, will provide a greater global outlook into ecology and evolution
Pandora's flying box - Borrelia burgdorferi sensu lato prevalence in Ixodes species from birds throughout Europe
Birds are important hosts for ticks and may act as reservoirs for several zoonotic pathogens. Because of their high mobility, especially of the long distance migratory species, they can act as dispersers for ticks and pathogens, ultimately affecting their distribution and phylogeography.N/
Proper Protein Glycosylation Promotes Mitogen-Activated Protein Kinase Signal Fidelity
The ability of cells to sense and respond appropriately to changing environmental conditions is often mediated by signal transduction pathways that employ mitogen-activated protein kinases (MAPKs). In the yeast Saccharomyces cerevisiae, the high osmolarity glycerol (HOG) and the filamentous growth (FG) pathways are activated following hyperosmotic stress and nutrient deprivation, respectively. Whereas the HOG pathway requires the MAPK Hog1, the FG pathway employs the MAPK Kss1. We conducted a comprehensive screen of nearly 5,000 gene deletion strains for mutants that exhibit inappropriate cross-talk between the HOG and FG pathways. We identified two novel mutants, mnn10Δ and mnn11Δ, that allow activation of Kss1 under conditions that normally stimulate Hog1. MNN10 and MNN11 encode mannosyltransferases that are part of the N-glycosylation machinery within the Golgi apparatus; deletion of either gene results in N-glycosylated proteins that have shorter mannan chains. Deletion of the cell surface mucin Msb2 suppressed the mnn11Δ phenotype, while mutation of a single glycosylation site within Msb2 was sufficient to confer inappropriate activation of Kss1 by salt stress. These findings reveal new components of the N-glycosylation machinery needed to ensure MAPK signaling fidelity