Characterisation of cv. Refosˇk (Vitis vinifera L.) by SSR markers

Fifty-five accessions of cv. Refošk from a clonal selection vineyard in the Karst district (Slovenia) were screened by 6 SSR markers in order to assess their uniformity. Two of the accessions showed different patterns (Clone 7 and Clone 50), while 53 accessions revealed identical SSR allelic profiles. Four of the uniform and the two different accessions were compared to 11 Refošk types from adjacent regions (Slovenia (Koper), Croatia and Italy) using 23 SSR markers. The SSR analysis revealed 7 identical genotypes (4 uniform Karst clones, one Italian and two Koper types), while three Koper (Slovenia) and three Italian types, as well as Teran from Croatia, showed genetic polymorphisms on an intra-varietal level. Clone 7, Clone 50 and Sladki Teran (Croatia) showed highly diverse genetic patterns from other types and should be considered different varieties. Comparative analysis allowed reliable construction of the predominant Refošk type grown in Slovenia.

Integrative transcriptomic analysis in human and mouse model of anaphylaxis identifies gene signatures associated with cell movement, migration and neuroinflammatory signalling

BackgroundAnaphylaxis is an acute life-threatening allergic reaction and a concern at a global level; therefore, further progress in understanding the underlying mechanisms and more effective strategies for diagnosis, prevention and management are needed.ObjectiveWe sought to identify the global architecture of blood transcriptomic features of anaphylaxis by integrating expression data from human patients and mouse model of anaphylaxis.MethodsBulk RNA-sequencings of peripheral whole blood were performed in: i) 14 emergency department (ED) patients with acute anaphylaxis, predominantly to Hymenoptera venom, ii) 11 patients with peanut allergy undergoing double-blind, placebo-controlled food challenge (DBPCFC) to peanut, iii) murine model of IgE-mediated anaphylaxis. Integrative characterisation of differential gene expression, immune cell-type-specific gene expression profiles, and functional and pathway analysis was undertaken.Results1023 genes were commonly and significantly dysregulated during anaphylaxis in ED and DBPCFC patients; of those genes, 29 were also dysregulated in the mouse model. Cell-type-specific gene expression profiles showed a rapid downregulation of blood basophil and upregulation of neutrophil signature in ED and DBPCFC patients and the mouse model, but no consistent and/or significant differences were found for other blood cells. Functional and pathway analysis demonstrated that human and mouse blood transcriptomic signatures of anaphylaxis follow trajectories of upregulation of cell movement, migration and neuroinflammatory signalling, and downregulation of lipid activating nuclear receptors signalling.ConclusionOur study highlights the matched and extensive blood transcriptomic changes and suggests the involvement of discrete cellular components and upregulation of migration and neuroinflammatory pathways during anaphylaxis

Large Scale Problems in Practice: The effect of dimensionality on the interaction among variables

The file attached to this record is the author's final peer reviewed version. The Publisher's final version can be found by following the DOI link.This article performs a study on correlation between pairs of variables in dependence on the problem dimensionality. Two tests, based on Pearson and Spearman coefficients, have been designed and used in this work. In total, $86$ test problems ranging between 10 and 1000 variables have been studied. If the most commonly used experimental conditions are used, the correlation between pairs of variables appears, from the perspective of the search algorithm, to consistently decrease. This effect is not due to the fact that the dimensionality modifies the nature of the problem but is a consequence of the experimental conditions: the computational feasibility of the experiments imposes an extremely shallow search in case of high dimensions. An exponential increase of budget and population with the dimensionality is still practically impossible. Nonetheless, since real-world application may require that large scale problems are tackled despite of the limited budget, an algorithm can quickly improve upon initial guesses if it integrates the knowledge that an apparent weak correlation between pairs of variables occurs, regardless the nature of the problem

Important and specific role for basophils in acute allergic reactions

IgE‐mediated allergic reactions involve the activation of effector cells, predominantly through the high‐affinity IgE receptor (FcεRI) on mast cells and basophils. Although the mast cell is considered the major effector cell during acute allergic reactions, more recent studies indicate a potentially important and specific role for basophils and their migration which occurs rapidly upon allergen challenge in humans undergoing anaphylaxis. We review the evidence for a role of basophils in contributing to clinical symptoms of anaphylaxis and discuss the possibility that basophil trafficking during anaphylaxis might be a pathogenic (to target organs) or protective (preventing degranulation in circulation) response. Finally, we examine the potential role of basophils in asthma exacerbations. Understanding the factors that regulate basophil trafficking and activation might lead to new diagnostic and therapeutic strategies in anaphylaxis and asthma

Preface: Special issue on “Understanding of evolutionary optimization behavior”, Part 1

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