COMPORTAMIENTO FISIOLÓGICO POSTCOSECHA DE TALLOS FLORALES DE ROSA (Rosa hybrida L.) EN RESPUESTA AL FÓSFORO APLICADO EN PRECOSECHA

Durante el crecimiento y desarro- llo de las flores, la nutrición fosforada es un factor que puede influir en la vida en flore- ro. El objetivo de este trabajo fue evaluar el efecto de cinco niveles de fósforo (P): 0, 0.5, 1.0, 1.5 y 2.0 me·L-1 de P aplicados en precosecha, mediante un sistema hidropóni- co abierto, en el comportamiento fisiológico postcosecha de tallos florales de rosa (Rosa hybrida L.) ‘Classy’ y ‘Vega’. Las variables evaluadas durante la vida de florero de los tallos florales fueron: transpiración foliar y floral, potencial de turgencia foliar y de pétalos. Además, se evaluó la concentración de antocianinas en pétalos al momento de la cosecha. Las aplicaciones de P en precose- cha tuvieron efecto significativo únicamente en la transpiración foliar; sin embargo, se demostró que la mayor vida en florero (15.8 días) de ‘Classy’ en comparación con ‘Vega’ (11.7 días) estuvo directamente asociada con mayores potenciales de turgencia foliar y de pétalos, mayor tasa transpiratoria floral y menor tasa transpiratoria foliar en ‘Classy’

Respuesta conductual de Aedes aegypti (Linneaus, 1762) frente adulticidas piretroides de uso frecuente en Salud Pública

Objetivos. Evaluar la respuesta conductual de la generación F1 de Aedes aegyti (L) colectados en el área metropolitana de Monterrey (Nuevo León, México) frente a tres adulticidas piretroides de uso frecuente en salud pública. Materiales y métodos. Se utilizó un sistema modular novedoso denominado HITSS (High-Throughput Screening System), para evaluar dos respuestas de comportamiento (irritación de contacto y repelencia espacial), así como la toxicidad de tres insecticidas DDT, permetrina y bifentrina a diferentes concentraciones (0,025, 0,25, 25 y 250 nmol/cm2). Resultados. En la concentración 2,5 nmol/cm2, el DDT (4,3 ± 2,4) y la permetrina (8,0 ± 1,4) son los insecticidas que tienen mayor efecto irritante (p<0,05); la bifentrina requiere dosis 20 veces más alta para lograr efectos similares. En repelencia espacial, los tres insecticidas evaluados producen respuestas similares en todas las concentraciones; para DDT de 7 a 14%; permetrina de 9 a 15% y bifentrina de 19 a 27%. La permetrina y bifentrina a concentraciones 0,025 nmol/cm2, producen efectos knockdown superiores a 34%, con una mortalidad 19%, el DDT requiere concentraciones diez veces más alta para lograr efectos similares. Conclusiones. El sistema HITTS puede ser usado para evaluar la respuesta conductual frente a insecticidas

Hydrogenation of an iridium-coordinated imidazol-2-ylidene ligand fragment

An iridium complex featuring a metalated lutidine-derived CNP ligand reacts with KOt Bu to yield a dimeric species with the two metal centers bound to the opposite ligands through the CHN arms. Furthermore, reaction with H2 in the presence of KOt Bu of the same iridium derivative results in the hydrogenation of the –CHQCH– imidazolylidene moiety of the complex. NMR spectroscopy monitoring of the latter reaction supports the initial formation of a dihydride iridium complex containing an imidazolylidene ligand fragment that is hydrogenated after prolonged reaction time.Ministerio de Economía y Competitividad de España (MINECO) y fondos FEDER de la Unión Europea-CTQ2015-69568-P, CTQ2016-80814-R y CTQ2016- 81797-RED

The Respiratory Exchange Ratio is Associated with Fitness Indicators Both in Trained and Untrained Men: A Possible Application for People with Reduced Exercise Tolerance

Background The respiratory exchange ratio (RER) indirectly shows the muscle's oxidative capacity to get energy. Sedentarism, exercise and physically active lifestyles modify it. For that reason, this study evaluates the associations between RER during sub-maximum exercise and other well established fitness indicators (body fat, maximum heart rate, maximum O 2 uptake, workload, and lactate threshold), in physically active trained and untrained men. Methods The RER, O 2 uptake and blood lactate were measured in eight endurance trained and eight untrained men (age, 22.9 ± 4.5 vs. 21.9 ± 2.8 years; body mass, 67.1 ± 5.4 vs. 72.2 ± 7.7 kg; body fat, 10.6 ± 2.4% vs. 16.6 ± 3.8% and maximum O2 uptake, 68.9 ± 6.3 vs. 51.6 ± 5.8 ml · kg −1 · min −1 ), during maximum exercise test and during three different sub-maximum exercises at fixed workload: below, within or above the lactate threshold. Results Endurance trained men presented higher O 2 uptake, lower blood lactate concentrations and lower RER values than those in untrained men at the three similar relative workloads. Even though with these differences in RER, a strong association (p < 0.05) of RER during sub-maximum exercise with the other well established fitness indicators was observed, and both maximum O 2 uptake and lactate threshold determined more than 57% of its variance (p < 0.05). Conclusions These data demonstrate that RER measurement under sub-maximum exercise conditions was well correlated with other established physical fitness indicators, despite training condition. Furthermore, the results suggest that RER could help obtain an easy approach of fitness status under low exercise intensity and could be utilized in subjects with reduced exercise tolerance

Reproducción del falso vampiro lanudo Chrotopterus auritus (Chiroptera: Phyllostomidae) en un bosque tropical húmedo de la costa pacífica de Guatemala

We documented one female Chrotopterus auritus in a lactation state with her calf present in the ventral part of her body. The specimen was captured with a harp trap in a humid tropical forest of the Pacific coast of Guatemala, within private natural reserve Finca Patrocinio, Quetzaltenango, Guatemala on May 29th, 2017. There is little information about this species’ biology, especially within Guatemala, which reflects the importance of further studies on the wild behavior of this species.Documentamos una hembra de Chrotopterus auritus en estado de lactancia con su cría presente en la parte ventral de su cuerpo. El espécimen fue capturado con una trampa de arpa en un bosque tropical húmedo de la costa del Pacífico de Guatemala, dentro de la reserva natural privada Finca Patrocinio, Quetzaltenango, Guatemala el 29 de mayo de 2017. Hay poca información sobre la biología de esta especie, especialmente en Guatemala, lo que refleja la importancia de nuevos estudios sobre el comportamiento silvestre de esta especie

Servicio de Control Microbiológico (SCM), como Estrategia Didáctica para el laboratorio de Tecnología de Alimentos [Microbiological Control Service, as Didactic Strategy for Food Technology Lab]

Servicio de Control Microbiológico (SCM) es una Estrategia Didáctica dirigida a alumnos de Laboratorios de: Tecnología de Alimentos (LABTEC) y Microbiología de Alimentos (LMA), en la Facultad de Química (FQ) de la Universidad Nacional Autónoma de México (UNAM). Se basa en el desempeño de roles profesionales, donde alumnos de LABTEC que elaboran alimentos actúan como productores que requieren análisis microbiológicos; los alumnos de LMA actúan como proveedores del servicio de control microbiológico. Se elaboran solicitudes y reportes formales, basados en normas de referencia, para que se pueda concluir sobre la calidad higiénica del alimento. La estrategia ha permitido a los participantes, una experiencia muy cercana al ejercicio profesional, para evaluar buenas prácticas de manufactura, trabajo individual y en equipo. Todos desarrollaron habilidades de comunicación profesional, solución de problemas y conciencia sobre la importancia de la calidad higiénica de los alimentos. El control microbiológico en LABTEC se ha realizado sin recursos adicionales, pues se aplican aquellos destinados a las prácticas de LMA. Además de la experiencia de aprendizaje, se está generando mejora continua en ambas asignaturas. [Microbiological control service (SCM) as a didactic strategy is aimed to those students in laboratories of food technology (LABTEC) and food microbiology (LMA), at the Universidad Nacional Autónoma de México’s School of Chemistry (FQ). It is based in professional role playing; LABTEC students elaborate the products and act as food producers in need of microbiological analysis; LMA students perform as service providers, for microbiological control. They elaborate formal requests and microbiological analysis report, based on mandatory standards, which can lead to conclusions about product’s hygienic quality. The strategy has given students an experience close to professional practice, to evaluate good manufacturing practices, individual and team work. All developed professional communication skills, problems identification and proposals for solving them, as well as consciousness about the importance of hygienic quality of food. The microbiological control in LABTEC has been carried out without the need for additional resources, since those intended for LMA practices are applied to these real samples. The experience is generating continuous improvement in both courses.

Human immunodeficiency virus/hepatits C virus coinfection in Spain: Elimination is feasible, but the burden of residual cirrhosis will be significant

Background We assessed the prevalence of antibodies against hepatitis C virus (HCV-Abs) and active HCV infection in patients infected with human immunodeficiency virus (HIV) in Spain in 2016 and compared the results with those of similar studies performed in 2002, 2009, and 2015. Methods The study was performed in 43 centers during October-November 2016. The sample was estimated for an accuracy of 2% and selected by proportional allocation and simple random sampling. During 2016, criteria for therapy based on direct-acting antiviral agents (DAA) were at least significant liver fibrosis, severe extrahepatic manifestations of HCV, and high risk of HCV transmissibility. Results The reference population and the sample size were 38904 and 1588 patients, respectively. The prevalence of HCV-Abs in 2002, 2009, 2015, and 2016 was 60.8%, 50.2%, 37.7%, and 34.6%, respectively (P trend <.001, from 2002 to 2015). The prevalence of active HCV in 2002, 2009, 2015, and 2016 was 54.0%, 34.0%, 22.1%, and 11.7%, respectively (P trend <.001). The anti-HCV treatment uptake in 2002, 2009, 2015, and 2016 was 23.0%, 48.0%, 59.3%, and 74.7%, respectively (P trend <.001). In 2016, HCV-related cirrhosis was present in 7.6% of all HIV-infected individuals, 15.0% of patients with active HCV, and 31.5% of patients who cleared HCV after anti-HCV therapy. Conclusions Our findings suggest that with universal access to DAA-based therapy and continued efforts in prevention and screening, it will be possible to eliminate active HCV among HIV-infected individuals in Spain in the short term. However, the burden of HCV-related cirrhosis will continue to be significant among HIV-infected individuals

Relationships between heavy metal concentrations in three different body fluids and male reproductive parameters: a pilot study

<p>Abstract</p> <p>Background</p> <p>Animal studies have shown the reproductive toxicity of a number of heavy metals. Very few human observational studies have analyzed the relationship between male reproductive function and heavy metal concentrations in diverse biological fluids.</p> <p>Methods</p> <p>The current study assessed the associations between seminal and hormonal parameters and the concentration of the 3 most frequent heavy metal toxicants (lead, cadmium and mercury) in three different body fluids. Sixty one men attending infertility clinics that participated in a case-control study to explore the role of environmental toxins and lifestyles on male infertility were analyzed. Concentration of lead, cadmium and mercury were measured in blood and seminal plasma and whole blood using anodic stripping voltammetry and atomic absorption spectrophotometry. Serum samples were analyzed for follicle-stimulating hormone, luteinizing hormone and testosterone. Semen analyses were performed according to World Health Organization criteria. Mann-Whitney test and Spearman's rank correlations were used for unadjusted analyses. Multiple linear regression models were performed controlling for age, body mass index and number of cigarettes per day.</p> <p>Results</p> <p>There were no significant differences between cases and controls in the concentrations of heavy metals in any of the three body fluids. In multivariate analyses using all subjects no significant associations were found between serum hormone levels and metal concentrations. However there was a significant positive association between the percentage of immotile sperms and seminal plasma levels of lead and cadmium.</p> <p>Conclusions</p> <p>Our results suggest that the presence of lead and cadmium in the reproductive tract of men may be related to a moderate alteration of their seminal parameters.</p

Detection of kinase domain mutations in BCR::ABL1 leukemia by ultra-deep sequencing of genomic DNA

The screening of the BCR::ABL1 kinase domain (KD) mutation has become a routine analysis in case of warning/failure for chronic myeloid leukemia (CML) and B-cell precursor acute lymphoblastic leukemia (ALL) Philadelphia (Ph)-positive patients. In this study, we present a novel DNA-based next-generation sequencing (NGS) methodology for KD ABL1 mutation detection and monitoring with a 1.0E−4 sensitivity. This approach was validated with a well-stablished RNA-based nested NGS method. The correlation of both techniques for the quantification of ABL1 mutations was high (Pearson r = 0.858, p < 0.001), offering DNA-DeepNGS a sensitivity of 92% and specificity of 82%. The clinical impact was studied in a cohort of 129 patients (n = 67 for CML and n = 62 for B-ALL patients). A total of 162 samples (n = 86 CML and n = 76 B-ALL) were studied. Of them, 27 out of 86 harbored mutations (6 in warning and 21 in failure) for CML, and 13 out of 76 (2 diagnostic and 11 relapse samples) did in B-ALL patients. In addition, in four cases were detected mutation despite BCR::ABL1 < 1%. In conclusion, we were able to detect KD ABL1 mutations with a 1.0E−4 sensitivity by NGS using DNA as starting material even in patients with low levels of disease.Tis project was funded in part by CRIS CANCER FOUNDATION

Oral health service utilization by elderly beneficiaries of the Mexican Institute of Social Security in México city

<p>Abstract</p> <p>Background</p> <p>The aging population poses a challenge to Mexican health services. The aim of this study is to describe recent oral health services utilization and its association with socio-demographic characteristics and co-morbidity in Mexican Social Security beneficiaries 60 years and older.</p> <p>Methods</p> <p>A sample of 700 individuals aged 60+ years was randomly chosen from the databases of the Mexican Institute of Social Security (IMSS). These participants resided in the southwest of Mexico City and made up the final sample of a cohort study for identifying risk factors for root caries in elderly patients. Sociodemographic variables, presence of cognitive decline, depression, morbidity, medication consumption, and utilization of as well as reasons for seeking oral health services within the past 12 months were collected through a questionnaire. Clinical oral assessments were carried out to determine coronal and root caries experience.</p> <p>Results</p> <p>The sample consisted of 698 individuals aged 71.6 years on average, of whom 68.3% were women. 374 participants (53.6%) had made use of oral health services within the past 12 months. 81% of those who used oral health services sought private medical care, 12.8% sought social security services, and 6.2% public health services. 99.7% had experienced coronal caries and 44.0% root caries. Female sex (OR = 2.0), 6 years' schooling or less (OR = 1.4), and caries experience in more than 22 teeth (OR = 0.6) are factors associated with the utilization of these services.</p> <p>Conclusion</p> <p>About half the elderly beneficiaries of social security have made use of oral health services within the past 12 months, and many of them have to use private services. Being a woman, having little schooling, and low caries experience are factors associated with the use of these services.</p