8 research outputs found
Distribution and molecular characteristics of rickettsiae found in ticks across Central Mongolia
Coupled cell networks are target cells of inflammation, which can spread between different body organs and develop into systemic chronic inflammation
THE INCIDENCE OF PARTIAL EDENTULISM AND DENTURE STATUS AMONG MONGOLIANS
<p><strong>Abstract</strong></p><p><strong>Introduction: </strong>Since 2013, the prevalence of tooth loss among adults in rural areas has not been studied in Mongolia. Therefore, our goal of the survey was to determine the level of tooth loss and denture needs among the population of Mongolia's. <strong>Materials and Methods: </strong>According to the geographical region of Mongolia, 1638 subjects over 18 years old were examined from target provinces and their partial edentulous status and denture status were determined. <strong>Results: </strong>The prevalence of partial edentulous level in total subjects was 1478 (90.2%), and partial edentulous level was high (93.5%) in over < 45 year-olds. The incidence of Kennedy's class in both arches was 880 (36.0%), Class III was 853 (34.8%), and Class II was 675 (27.6%), respectively. Class IV was lower at 40 (1.6%) in both dental arches (P< 0.01). Evaluating denture status in people: 435 (67.8%) have acrylic dentures and 171 (26.6%) have flexible dentures. The number of subjects who needed prosthetic treatment was 861 (57.3%).<strong> Conclusion: </strong>The level and pattern of partial edentulism among adults Mongolia were high, and the level and pattern of tooth loss were significantly associated with age groups. Prosthetic needs among rural people are high, and all these findings are also being considered in oral health promotion strategies designed to reduce tooth loss in Mongolia's adults. </p>
Natural Anaplasma phagocytophilum infection in ticks from a forest area of Selenge province, Mongolia
Anaplasma phagocytophilum is a zoonotic agent of public health importance, infecting both humans and animals. An investigation of the presence of Anaplasma phagocytophilum as well as Anaplasma platys was conducted in a forest area of Selenge province, Mongolia, where ticks are widely distributed and tick-borne diseases are highly endemic. Ticks were collected and tested using polymerase chain reaction based on groEL methodology. Anaplasma phagocytophilum was detected in 14 (6%) of Ixodes persulcatus ticks and four (1%) Dermacentor nuttalli ticks; infection of Anaplasma platys was detected in 1% of Ixodes persulcatus ticks and 10% of Dermacentor nuttalli ticks. The phylogenetic tree showed that the Anaplasma phagocytophilum clustered with the Russian group, most likely due to similar geographical locations. This finding is significant for both veterinary and public health officials given that these agents can cause both animal and human illness
In-house Production Method for DNA Ladders to Determine Nucleotide Fragment Sizes up to 1500 Base Pairs
The human genome project was recently completed after running for 15 years and revealed the presence of 30,000 genes in the human genome with a total nucleotide length of 3.2 billion base pairs (bp). Many novel methods and techniques have been developed in the field of molecular biology and molecular genetics as a result of intensive research, where basic analysis is impossible without the use of DNA size markers or DNA ladders. This research aimed to establish an in-house method to produce DNA size markers detecting up to 1500 bp size. DNA size markers are commonly used consumables in molecular biology laboratories. In this study, we report preparation of a DNA size marker consisting of 12 fragments from 100 to 1500 bp. DNA fragments were amplified by PCR and PCR products were then ligated in the cloning vector pDYNE TA V2. Our procedure for DNA size marker production could be simple, time saving, and inexpensive.nbs