A clinically relevant model of osteoinduction: a process requiring calcium phosphate and BMP/Wnt signalling

In this study, we investigated a clinically relevant model of in vivo ectopic bone formation utilizing human periosteum derived cells (HPDCs) seeded in a Collagraft carrier and explored the mechanisms by which this process is driven. Bone formation occurred after eight weeks when a minimum of one million HPDCs was loaded on Collagraft carriers and implanted subcutaneously in NMRI nu/nu mice. De novo bone matrix, mainly secreted by the HPDCs, was found juxta-proximal of the calcium phosphate (CaP) granules suggesting that CaP may have triggered the 'osteoinductive program'. Indeed, removal of the CaP granules by ethylenediaminetetraacetic acid decalcification prior to cell seeding and implantation resulted in loss of bone formation. In addition, inhibition of endogenous bone morphogenetic protein and Wnt signalling by overexpression of the secreted antagonists Noggin and Frzb, respectively, also abrogated osteoinduction. Proliferation of the engrafted HPDCs was strongly reduced in the decalcified scaffolds or when seeded with adenovirus-Noggin/Frzb transduced HPDCs indicating that cell division of the engrafted HPDCs is required for the direct bone formation cascade. These data suggest that this model of bone formation is similar to that observed during physiological intramembranous bone development and may be of importance when investigating tissue engineering strategies.Published versio

Ultrashort Lifetime Expansion for Indirect Resonant Inelastic X-ray Scattering

In indirect resonant inelastic X-ray scattering (RIXS) an intermediate state is created with a core-hole that has a ultrashort lifetime. The core-hole potential therefore acts as a femtosecond pulse on the valence electrons. We show that this fact can be exploited to integrate out the intermediate states from the expressions for the scattering cross section. By this we obtain an effective scattering cross section that only contains the initial and final scattering states. We derive in detail the effective cross section which turns out to be a resonant scattering factor times a linear combination of the charge response function $S({\bf q},\omega)$ and the dynamic longitudinal spin density correlation function. This result is asymptotically exact for both strong and weak local core-hole potentials and ultrashort lifetimes. The resonant scattering pre-factor is shown to be weakly temperature dependent. We also derive a sum-rule for the total scattering intensity and generalize the results to multi-band systems. One of the remarkable outcomes is that one can change the relative charge and spin contribution to the inelastic spectral weight by varying the incident photon energy.Comment: 9 pages, 3 figures embedde

Resonant Inelastic X-ray Scattering on Spin-Orbit Coupled Insulating Iridates

We determine how the elementary excitations of iridium-oxide materials, which are dominated by a strong relativistic spin-orbit coupling, appear in Resonant Inelastic X-ray Scattering (RIXS). Whereas the RIXS spectral weight at the L2 x-ray edge vanishes, we find it to be strong at the L3-edge. Applying this to Sr2IrO4, we observe that RIXS, besides being sensitive to local doublet to quartet transitions, meticulously maps out the strongly dispersive delocalized excitations of the low-lying spin-orbit doublets.Comment: 4 pages, 3 figure

Numerical simulations of transition due to isolated roughness elements at Mach 6

An accurate prediction of transition onset behind an isolated roughness element has not yet been established. This is particularly important in hypersonic flow, where transition is accompanied by increased surface heating. In the present contribution, a number of direct numerical simulations have been performed of a Mach 6 boundary layer over a flat plate with isolated roughness elements. The effects of roughness shape, planform, ramps, and freestream disturbance levels on instability growth and transition onset are investigated. It is found that the frontal shape has a large effect on the transition onset, which is in agreement with previous studies, whereas the roughness element planform has a marginal influence. A new result is that the roughness shape in the streamwise direction (in particular, the aft section) is also an important characteristic, since an element with a ramped-down aft section allows the detached shear layer to spread out and weaken, leading to a lower instability growth rate. Above a critical value, the instability growth rate is found to be correlated with the amplitude of the low-speed streak formed by the roughness element, suggesting that a more physically based transition criterion should take account of the local liftup effect of the particular roughness shape.<br/

Potential dietary feed additives with antibacterial effects and their impact on performance of weaned piglets : a meta-analysis

This meta-analysis evaluated the use of potential dietary feed additives (pDFA) with antibacterial effects and their impact on the perfomance of weaned piglets. Twenty-three peer-reviewed in vivo studies, comprising 50 trials, were identified between January 2010 and January 2017. The pDFA in these studies could be grouped in 5 classes: antimicrobial peptides, chitosan, lysozyme, medium chain fatty acids/triglycerides and plant extracts. Mixed-effect meta-analyses with type of pDFA as fixed effect were performed for the growth parameters 'average daily gain' (ADG) and 'feed conversion ratio' (FCR), which are the two most important and used economic performance parameters for farmers. For each class of pDFA, results of the meta-analysis showed significantly higher average daily gain in the group with pDFA compared to the negative control group, while no significant difference with the positive control group was observed. Furthermore, a positive effect on FCR was found, i.e. significantly less feed was needed to gain 1 kg of body weight in the group with pDFA compared to the negative control group. No significant differences with positive control groups were observed for each class of pDFA, except for plant extracts, where the FCR was also significantly reduced in the treatment group. These results suggest that pDFA could reduce the use of antimicrobials without significant negative effects on performance indicators

Symbolic Reachability Analysis of B through ProB and LTSmin

We present a symbolic reachability analysis approach for B that can provide a significant speedup over traditional explicit state model checking. The symbolic analysis is implemented by linking ProB to LTSmin, a high-performance language independent model checker. The link is achieved via LTSmin's PINS interface, allowing ProB to benefit from LTSmin's analysis algorithms, while only writing a few hundred lines of glue-code, along with a bridge between ProB and C using ZeroMQ. ProB supports model checking of several formal specification languages such as B, Event-B, Z and TLA. Our experiments are based on a wide variety of B-Method and Event-B models to demonstrate the efficiency of the new link. Among the tested categories are state space generation and deadlock detection; but action detection and invariant checking are also feasible in principle. In many cases we observe speedups of several orders of magnitude. We also compare the results with other approaches for improving model checking, such as partial order reduction or symmetry reduction. We thus provide a new scalable, symbolic analysis algorithm for the B-Method and Event-B, along with a platform to integrate other model checking improvements via LTSmin in the future

Remote collaboration and innovative performance:the moderating role of R&D intensity

Collaboration with geographically distant partners may enhance a firm’s innovative performance. In practice, however, this may be complicated as personal contacts are more limited so that effective search and transfer of remote partners’ tacit knowledge is hampered. We tested the potential moderating role of R&D intensity which, by indicating technology-oriented absorptive capacity, may mitigate the problems associated with remote collaboration. Drawing on survey data of 248 high-tech small firms, we find that remote collaboration is positively related with innovation performance, but at low R&D intensity, the relationship vanishes

Imaging Electron Wave Functions of Quantized Energy Levels in Carbon Nanotubes

Carbon nanotubes provide a unique system to study one-dimensional quantization phenomena. Scanning tunneling microscopy is used to observe the electronic wave functions that correspond to quantized energy levels in short metallic carbon nanotubes. Discrete electron waves are apparent from periodic oscillations in the differential conductance as a function of the position along the tube axis, with a period that differs from that of the atomic lattice. Wave functions can be observed for several electron states at adjacent discrete energies. The measured wavelengths are in good agreement with the calculated Fermi wavelength for armchair nanotubes.Comment: 11 pages, 4 figures in seperate PDF fil