369 research outputs found
Demographic analysis reveals gradual senescence in the flatworm Macrostomum lignano
Free-living flatworms ("Turbellaria") are appropriate model organisms to gain better insight into the role of stem cells in ageing and rejuvenation. Ageing research in flatworms is, however, still scarce. This is partly due to culture difficulties and the lack of a complete set of demographic data, including parameters such as median lifespan and age-specific mortality rate. In this paper, we report on the first flatworm survival analysis. We used the species Macrostomum lignano, which is an emerging model for studying the reciprocal influence between stem cells, ageing and rejuvenation. This species has a median lifespan of 205 Β± 13 days (average Β± standard deviation [SD]) and a 90th percentile lifespan of 373 Β± 32 days. The maximum lifespan, however, is more than 745 days, and the average survival curve is characterised by a long tail because a small number of individuals lives twice as long as 90% of the population. Similar to earlier observations in a wide range of animals, in M. lignano the age-specific mortality rate increases exponentially, but levels off at the oldest ages. To compare the senescence of M. lignano with that of other ageing models, we determined the mortality rate doubling time, which is 0.20 Β± 0.02 years. As a result, we can conclude that M. lignano shows gradual senescence at a rate similar to the vertebrate ageing models Rattus norvegicus and Mus musculus. We argue that M. lignano is a suitable model for ageing and rejuvenation research, and especially for the role of stem cells in these processes, due to its accessible stem cell system and regeneration capacity, and the possibility of combining stem cell studies with demographic analyses
The role of macrofauna in the functioning of a sea floor: is there any seasonal, density or functional identity effect?
Macrobenthos influences rates and intensities of benthic processes. The way in which these processes are affected depends on their densities and functional characteristics in terms of sediment reworking (bioturbation and bio-irrigation). This study focuses on the importance of three different functional groups (FG) of macrobenthos in the ecosystem processes of the Western Coastal Banks area (Belgian Part of the North Sea). Macrobenthic activity depends on temperature and food availability. Therefore two lab experiments were performed: one before sedimentation of the phytoplankton bloom (spring: low food availability and temperature) and one when organic matter had been settled on the sea bottom (late summer: high food availability and higher temperatures). Single - species treatments of key-species belonging to three different functional groups were added to microcosms at three density levels (average natural, lower and very low) to account for possible density declines. These species are the bivalve Abra alba (FG: biodiffuser), the tube-building polychaete Lanice conchilega (FG: piston-pumper) andthe predatory polychaete Nephthy sp. (FG: regenerator/gallery-diffuser).In both winter - and summertime, L. conchilega had a more pronounced influence on oxygen consumption and release Nephtys sp.. Abra alba appeared to be a more effective sediment reworker than Nephtys sp. in both seasons. In addition, ecosystem functioning (as oxygen consumption by the sediment community and bioturbation) seems to be related to animal densities. As such, a decline of densities (due to anthropogenic or natural disturbances) most probably will decrease the rates of ecosystem functioning in theWestern Coastal Banks area
A multi-parent recombinant inbred line population of C. elegans allows identification of novel QTLs for complex life history traits
Background
The nematode Caenorhabditis elegans has been extensively used to explore the relationships between complex traits, genotypes, and environments. Complex traits can vary across different genotypes of a species, and the genetic regulators of trait variation can be mapped on the genome using quantitative trait locus (QTL) analysis of recombinant inbred lines (RILs) derived from genetically and phenotypically divergent parents. Most RILs have been derived from crossing two parents from globally distant locations. However, the genetic diversity between local C. elegans populations can be as diverse as between global populations and could thus provide means of identifying genetic variation associated with complex traits relevant on a broader scale.
Results
To investigate the effect of local genetic variation on heritable traits, we developed a new RIL population derived from 4 parental wild isolates collected from 2 closely located sites in France: Orsay and Santeuil. We crossed these 4 genetically diverse parental isolates to generate a population of 200 multi-parental RILs and used RNA-seq to obtain sequence polymorphisms identifying almost 9000 SNPs variable between the 4 genotypes with an average spacing of 11 kb, doubling the mapping resolution relative to currently available RIL panels for many loci. The SNPs were used to construct a genetic map to facilitate QTL analysis. We measured life history traits such as lifespan, stress resistance, developmental speed, and population growth in different environments, and found substantial variation for most traits. We detected multiple QTLs for most traits, including novel QTLs not found in previous QTL analysis, including those for lifespan and pathogen responses. This shows that recombining genetic variation across C. elegans populations that are in geographical close proximity provides ample variation for QTL mapping.
Conclusion
Taken together, we show that using more parents than the classical two parental genotypes to construct a RIL population facilitates the detection of QTLs and that the use of wild isolates facilitates the detection of QTLs. The use of multi-parent RIL populations can further enhance our understanding of local adaptation and life history trade-offs
Comparison of the haemostatic properties of conventional monopolar and bipolar transurethral resection of the prostate in patients on oral anticoagulants
A b s t r a c t I In nt tr ro od du uc ct ti io on n: : The aim of study is comparing the haemostatic properties of conventional monopolar resection (TURP) and bipolar transurethral resection in saline (TURIS) of the prostate in patients under chronic oral anticoagulants. M Ma at te er ri ia al l a an nd d m me et th ho od ds s: : Out of a cohort group of 550 endoscopic resections for bladder outlet obstruction, 176 patients on chronic oral anticoagulant therapy required endoscopic resection either by monopolar TURP or bipolar TURIS technology. Changes in haemoglobin, blood transfusion, and clot retention were compared between both groups. R Re es su ul lt ts s: : Mean postoperative change in haemoglobin level was -1.21 Β±0.92 mg/dl in the TURP group compared to -1.29 Β±0.99 mg/dl in the TURIS group (p = 0.603). The need for blood transfusions and the mean numbers of units transfused did not significantly differ between the 2 groups. Clot retention appeared in 12 patients (15%) in the TURP group compared to 13 patients (13%) in the TURIS group (p = 0.828). C Co on nc cl lu us si io on ns s: : Despite promising experimental results of better haemostasis and deeper coagulation depth, bipolar technology does not permit one to reduce the amount of blood loss when compared to patients treated by conventional monopolar technology in this study group of patients on oral anticoagulation therapy. Patients on oral anticoagulants suffer more incidents of clot retention, which sometimes results in re-hospitalisation. K Ke ey y w wo or rd ds s: : prostate, bipolar, transurethral resection of prostate
Effect of Concomitant Icosapent Ethyl (Eicosapentaenoic Acid Ethyl Ester) on the Pharmacokinetics of Atorvastatin
Understanding benthic, pelagic and airborne ecosystem interactions in shallow coastal seas. "Westbanks": Final Report
Organism-sediment interactions govern post-hypoxia recovery of ecosystem functioning
Hypoxia represents one of the major causes of biodiversity and ecosystem functioning loss for coastal waters. Since eutrophication-induced hypoxic events are becoming increasingly frequent and intense, understanding the response of ecosystems to hypoxia is of primary importance to understand and predict the stability of ecosystem functioning. Such ecological stability may greatly depend on the recovery patterns of communities and the return time of the system properties associated to these patterns. Here, we have examined how the reassembly of a benthic community contributed to the recovery of ecosystem functioning following experimentally-induced hypoxia in a tidal flat. We demonstrate that organism-sediment interactions that depend on organism size and relate to mobility traits and sediment reworking capacities are generally more important than recovering species richness to set the return time of the measured sediment processes and properties. Specifically, increasing macrofauna bioturbation potential during community reassembly significantly contributed to the recovery of sediment processes and properties such as denitrification, bedload sediment transport, primary production and deep pore water ammonium concentration. Such bioturbation potential was due to the replacement of the small-sized organisms that recolonised at early stages by large-sized bioturbating organisms, which had a disproportionately stronger influence on sediment. This study suggests that the complete recovery of organism-sediment interactions is a necessary condition for ecosystem functioning recovery, and that such process requires long periods after disturbance due to the slow growth of juveniles into adult stages involved in these interactions. Consequently, repeated episodes of disturbance at intervals smaller than the time needed for the system to fully recover organism-sediment interactions may greatly impair the resilience of ecosystem functioning.
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