Increase of metallothionein-immunopositive chloride cells in the gills of brown trout and rainbow trout after exposure to sewage treatment plant effluents

Metallothionein, a biomarker of exposure and toxicity of heavy metals, has been detected in the gills of brown trout (Salmo trutta fario L.) and rainbow trout (Oncorhynchus mykiss Richardson) by means of immunohistochemistry. A very prominent labelling of chloride cells was found after exposure to diluted sewage plant effluents. No significant increase was observed in either the number of labelled cells or their labelling intensity after exposure to water of a polluted river compared to fish kept in tap water. These results do not correlate with findings of a histopathological study, suggesting that the metal levels at the sewage treatment plant were too low to produce gross histopathology. A comparison between the species indicated that the rainbow trout showed a generally higher metallothionein expression than the brown trout

Distribution of mutational fitness effects and of epistasis in the 5' untranslated region of a plant RNA virus

[Background[ Understanding the causes and consequences of phenotypic variability is a central topic of evolutionary biology. Mutations within non-coding cis-regulatory regions are thought to be of major effect since they affect the expression of downstream genes. To address the evolutionary potential of mutations affecting such regions in RNA viruses, we explored the fitness properties of mutations affecting the 5’-untranslated region (UTR) of a prototypical member of the picorna-like superfamily, tobacco etch virus (TEV). This 5’ UTR acts as an internal ribosomal entry site (IRES) and is essential for expression of all viral genes.[Results] We determined in vitro the folding of 5’ UTR using the selective 2’-hydroxyl acylation analyzed by primer extension (SHAPE) technique. Then, we created a collection of single-nucleotide substitutions on this region and evaluated the statistical properties of their fitness effects in vivo. We found that, compared to random mutations affecting coding sequences, mutations at the 5’ UTR were of weaker effect. We also created double mutants by combining pairs of these single mutations and found variation in the magnitude and sign of epistatic interactions, with an enrichment of cases of positive epistasis. A correlation exists between the magnitude of fitness effects and the size of the perturbation made in the RNA folding structure, suggesting that the larger the departure from the predicted fold, the more negative impact in viral fitness.[Conclusions] Evidence that mutational fitness effects on the short 5’ UTR regulatory sequence of TEV are weaker than those affecting its coding sequences have been found. Epistasis among pairs of mutations on the 5’ UTR ranged between the extreme cases of synthetic lethal and compensatory. A plausible hypothesis to explain all these observations is that the interaction between the 5’ UTR and the host translational machinery was shaped by natural selection to be robust to mutations, thus ensuring the homeostatic expression of viral genes even at high mutation rates.This work was supported by grant BFU2012-30805 from the Spanish Ministry of Economy and Competitiveness (MINECO), grant PROMETEOII/2014/021 from Generalitat Valenciana and the EvoEvo (ICT610427) project from the European Commission 7th Framework Program. Publication fees have been partially paid by the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI).Peer reviewe

Atomic matter wave scanner

We report on the experimental realization of an atom optical device, that allows scanning of an atomic beam. We used a time-modulated evanescent wave field above a glass surface to diffract a continuous beam of metastable Neon atoms at grazing incidence. The diffraction angles and efficiencies were controlled by the frequency and form of modulation, respectively. With an optimized shape, obtained from a numerical simulation, we were able to transfer more than 50% of the atoms into the first order beam, which we were able to move over a range of 8 mrad.Comment: 4 pages, 4 figure

Permissive Controller Synthesis for Probabilistic Systems

We propose novel controller synthesis techniques for probabilistic systems modelled using stochastic two-player games: one player acts as a controller, the second represents its environment, and probability is used to capture uncertainty arising due to, for example, unreliable sensors or faulty system components. Our aim is to generate robust controllers that are resilient to unexpected system changes at runtime, and flexible enough to be adapted if additional constraints need to be imposed. We develop a permissive controller synthesis framework, which generates multi-strategies for the controller, offering a choice of control actions to take at each time step. We formalise the notion of permissivity using penalties, which are incurred each time a possible control action is disallowed by a multi-strategy. Permissive controller synthesis aims to generate a multi-strategy that minimises these penalties, whilst guaranteeing the satisfaction of a specified system property. We establish several key results about the optimality of multi-strategies and the complexity of synthesising them. Then, we develop methods to perform permissive controller synthesis using mixed integer linear programming and illustrate their effectiveness on a selection of case studies

Sum Rules and Moments of the Nucleon Spin Structure Functions

The nucleon has been used as a laboratory to investigate its own spin structure and Quantum Chromodynamics. New experimental data on nucleon spin structure at low to intermediate momentum transfers combined with existing high momentum transfer data offer a comprehensive picture of the transition region from the {\it confinement} regime of the theory to its {\it asymptotic freedom} regime. Insight for some aspects of the theory is gained by exploring lower moments of spin structure functions and their corresponding sum rules (i.e. the Gerasimov-Drell-Hearn, Bjorken and Burkhardt-Cottingham). These moments are expressed in terms of an operator product expansion using quark and gluon degrees of freedom at moderately large momentum transfers. The sum rules are verified to a good accuracy assuming that no singular behavior of the structure functions is present at very high excitation energies. The higher twist contributions have been examined through the moments evolution as the moments evolution as the momentum transfer varies from higher to lower values. Furthermore, QCD-inspired low-energy effective theories, which explicitly include chiral symmetry breaking, are tested at low momentum transfers. The validity of these theories is further examined as the momentum transfer increases to moderate values. It is found that chiral perturbation calculations agree reasonably well with the first moment of the spin structure function $g_1$ at momentum transfer of 0.1 GeV$^2$ but fail to reproduce the neutron data in the case of the generalized polarizability $\delta_{LT}$.Comment: 21 pages, 4 figures, review for Modern Physics Letters A. Minor modifications in text and improved quality for one figure. Corrected mistakes in section

The games plant viruses play

[EN] Mixed virus infections in plants are common in nature. The outcome of such virus–virus interactions ranges from cooperation and coexistence (synergism) to mutual exclusion (antagonism). A priori, the outcome of mixed infections is hard to predict. To date, the analyses of plant virus mixed infections were limited to reports of emerging symptoms and/or to qualitative, at best quantitative, descriptions of the accumulation of both viruses. Here, we show that evolutionary game theory provides an adequate theoretical framework to analyze mixed viral infections and to predict the long-term evolution of the mixed populations.This work was supported by the Spanish Secretaria de Estado de Investigacion, Desarrollo e Innovacion grant BFU2012-30805.Elena Fito, SF.; Bernet, GP.; Carrasco Jiménez, JL. (2014). The games plant viruses play. Current Opinion in Virology. 8:62-67. https://doi.org/10.1016/j.coviro.2014.07.0036267

Selective Harmonic Elimination Model Predictive Control for Multilevel Power Converters

© 2016 IEEE. In this study, a model predictive control (MPC) strategy that combines finite-control-set MPC with selective harmonic elimination (SHE) modulation pattern in its formulation is proposed to govern multilevel power converters. Based on a desired operating point for the system state (converter current reference), an associated predefined SHE voltage pattern is obtained as a required steady-state control input reference. Then, the cost function is formulated with the inclusion of both system state and control input references. According with the proposed reference and cost function formulation, the predictive controller prefers to track the converter output current reference in transients, while preserving the SHE voltage pattern in steady state. Hence, as evidenced by experimental results, a fast dynamic response is obtained throughout transients while a predefined voltage and current spectrum with low switching frequency is achieved in steady state

The phase spiral in Gaia DR3

We aim to study the phase spiral in the Milky Way (MW) with Gaia DR3. We used an edge detection algorithm to find the border of the phase spiral, allowing us to robustly quantify its shape at different positions and for different selections. We calculated the time of onset of the phase-mixing by determining the different turns of the phase spiral and using the vertical frequencies from commonly used MW potential models. We find that the phase spiral extends down to $-1.2$ kpc in height below the plane (about 3 to 5 scale heights of the thin disc) and beyond $\pm 50$ km/s in $V_Z$. We see a secondary branch mostly at positive vertical velocities when coloured by azimuthal velocity and in the counts projection. We also find complex variations of the phase spirals with angular momentum and azimuth. All these possibly provide evidence of multiple perturbations (from different times or from different perturbers) and/or of the complexity of the phase mixing process. We detect the phase spiral from 6 to 11 kpc from the Galactic centre and find signatures of vertical asymmetries 1-2 kpc beyond this range. We measure small but clear variations with azimuth. When we determine the phase mixing times from the phase spiral at different angular momenta and using the different spiral turns (at different $Z$) we obtain inconsistent times with systematic differences (times increasing with $|L_Z|$ and with $|Z|$). Our determinations are mostly in the range of [0.3-0.9] Gyr, with an average of 0.5 Gyr. The inconsistencies do not change when using different usual potential models, different stellar distances or frequencies for different kinetic temperatures. They could stem from the inconsistency of potential models with the true MW, and from too simple modelling, in particular neglecting self-gravity, not considering the multiple perturbations and the interference with other processes.Comment: accepted for publication in A&