Radiosensitivitas dan Pengaruh Radiasi Sinar Gamma terhadap Pertumbuhan dan Produksi Tomat (Solanum lycopersicum L.)

This study aims to determine the radiosensitivity of tomato plants given by gamma ray radiation through the LD50 value, and to examine the effect of gamma ray irradiation dose on the growth and production characteristics of tomato plants. The research was carried out in March – August 2022 at the screenhouse and Laboratory of Physiology and Plant Breeding, Diponegoro University. The irradiation given at the National Nuclear Energy Agency of Indonesia, National Research and Innovation Agency, Jakarta. The study used a completely randomized design (CRD) with a dose of gamma ray radiation as a treatment, namely: (G0) Control, (G1) 125 Gy, (G2) 250 Gy, (G3) 375 Gy, and (G4) 500 Gy and consisted of 5 replicates. The results showed that the LD50 value of tomatoes was 460,23 Gy. Gamma rays had a significant effect on the growth and production characteristics of tomatoes, such as plant height, flowering age, harvest age, number, and weight per plant, but had no effect on stem diameter characters.Keywords: Irradiation, LD50, Gamma-ray, Tomat

No more recalcitrant: Chickpea regeneration and genetic transformation

Chickpea production is limited worldwide because of abiotic and biotic stresses. Efforts to overcome these production constraints through traditional breeding are difficult due to limited genetic variation. Novel regeneration is pre-requisite for genetic transformation offers the opportunity to overcome hybridization barriers and introduce novel genes for resistance. Although direct gene transfer via direct DNA transfer has been reported, Agrobacterium mediated transformation is the preferred method and standard protocols have been established for the production of transgenic plantlets derived from cocultivation of embryonic axes. This was soon adopted due to difficulties associated with regeneration of whole plants from callus. Only few reports have been reported using genetic transformation/transgene(s) against abiotic stress tolerance transgenic chickpea plants. Transgenic chickpea using bacterial codA gene tolerance against abiotic stresses have been developed. Chickpea improvement and application of genomics tools to the study of the chickpea genome will be enhanced through the use of genetic transformation