Plateau de microscopie électronique à transmission

National audiencePrésentation des offres de service, des équipements disponibles et des techniques mises en oeuvre sur le plateau de ME

Calcium-independent phospholipase A2 is involved in both the retrograde and anterograde traffic in mammary epithelial cells

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Src regulates Golgi structure and KDEL receptor-dependent retrograde transport to the endoplasmic reticulum

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Dictyostelium extracellular vesicles containing Hoechst 33342 transfer the dye into the nuclei of living cells: A fluorescence study

International audienceCells of the eukaryotic unicellular microorganism Dictyostelium discoideum are constitutively resistant to vital staining of their nuclei by the DNA-specific dye Hoechst 33342. By studying the mechanisms of this resistance, we evidenced that these cells expel vesicles containing the dye for detoxification (Tatischeff et al., Cell Mol Life Sci, 54: 476–87, 1998). The question to be addressed in the present work is the potential use of these extracellular vesicles as a biological drug delivery tool, using Hoechst 33342 as a model of a DNA-targeting drug. After cell growth with or without the dye, vesicles were prepared from the cell-free growth medium by differential centrifugation, giving rise to two types of vesicles. Negative staining electron microscopy showed their large heterogeneity in size. Using fluorescence techniques, data were obtained on the dye loading and its environment inside the vesicles. By UV video-microscopy, it was demonstrated that the dye-containing vesicles were able to deliver it into the nuclei of naive Dictyostelium cells, thus overcoming their constitutive resistance to the free dye. A vesicle-mediated dye-transfer into the nuclei of living human leukaemia multidrug resistant K562r cells was also observed