Investigation on Malondialdehyde, S100B, and Advanced Oxidation Protein Product Levels in Significant Hyperbilirubinemia and the Effect of Intensive Phototherapy on these Parameters

BackgroundThe parameters of oxidative stress [advanced oxidation protein products (AOPPs), malondialdehyde (MDA), and S100B] and the effect of intensive phototherapy (PT) on these parameters have not been studied extensively in newborns with significant hyperbilirubinemia (SH). We aimed to measure the levels of MDA, S100B, and AOPPs in newborns with SH, and to compare newborns with healthy control newborns without hyperbilirubinemia on the basis of these parameters of oxidative stress. In addition, we investigated the effect of intensive PT on these parameters during the treatment of SH and report our findings for the first time in the literature.MethodsThe study was performed in newborns (n = 62) who underwent intensive PT because of SH. Newborns without jaundice constituted the control group (n = 30). Both groups were compared with respect to demographic characteristics and biochemical (laboratory) parameters including MDA, AOPPs, and S100B. MDA, AOPPs, and S100B were also compared before and after intensive PT in the PT group. In the study group, a correlation analysis of demographic characteristics; MDA, AOPP, and S100B values; and changes occurring in MDA, AOPPs, and S100B values due to the effect of intensive PT was performed.ResultsSerum total bilirubin, S100B, and MDA levels in the PT group before performing PT were significantly higher than those in the control group. In newborns receiving PT serum total bilirubin, MDA and AOPP levels decreased significantly after intensive PT. In correlation analysis, a statistically significant negative correlation was found only between the amount of bilirubin decrease with PT and AOPP levels after PT in the study group.ConclusionWhether the significant decrease in MDA levels, which was higher prior to PT, is due to the decrease in serum bilirubin levels or due to the effect of intensive PT itself remains to be determined in further studies. The decrease in AOPP levels after PT implies that intensive PT has protective effects on oxidative stress

Bifosfonat Kullanımına Bağlı Olarak Maksillada Kemik Nekrozu Oluşumu: Bir Olgu Sunumu

Amaç: Bifosfonatlar, metastatik kemik kanserlerin önlenmesinde ve osteopörözün tedavisinde kullanılmaktadır. İntravenöz (i.v) bifosfonat (pamidronat, zoledronat) tedavisine bağlı olarak çene kemiklerinde osteonekroz gelişmektedir. Bu vaka raporu ile kliniğimize başvuran hastanın tedavisi sunulmuştur. Vaka Raporu: Kliniğimize başvuran 51 yaşındaki kadın hastada, uzun süreli intravenöz bifosfonat kullanımına bağlı sol maksilla molar bölgesinde asemptomatik osteonekroz görülmüştür. Hastaya gerekli debridman, sekestrasyon ve hiperbarik oksijen tedavisi uygulanmıştır. Hasta belirli aralıklarla takip edilmektedir. Sonuç: Bifosfonat kullanımına bağlı oluşan osteonekroz, i.v. bifosfonat kullanımının artmasına bağlı olarak daha sık görülmektedir. Bu nedenle bifosfonat tedavisi görecek olan hastaların oluşabilecek komplikasyonların engellenmesi amacıyla, tedavi öncesinde diş hekimine yönlendirilip, tüm dental tedavilerinin yapılması önerilmektedir

The local clinical validation of a new lithium heparin tube with a barrier: BD Vacutainer® Barricor LH Plasma tube

Introduction: Although serum-providing blood tubes with a barrier are still widely used due to their significant advantages, the use of blood tubes with a barrier to provide plasma is becoming widespread. We compared 22 analytes in a BD Vacutainer® Barricor LH Plasma tube for local clinical validation of this new lithium heparin tube with a barrier. Materials and methods: Samples from 44 volunteers were collected in different tubes (Becton Dickinson and Company): Z tube without additive (reference), clot-activator tube with gel (SST), lithium heparin tube without gel (LiH), and lithium heparin tube with barrier (Barricor). Analyte concentrations in different tubes were compared with the reference tube. All tubes were also evaluated according to additional testing (different centrifugation durations, blood-sampling techniques and individual differences). Results: Aspartate aminotransferase (AST), glucose (Glc), potassium (K), lactate dehydrogenase (LD), sodium (Na), and total protein (TP) had a significant bias in Barricor (9.19%, - 3.24%, - 4.88%, 21.60%, - 0.40%, 5.03%, respectively) relative to the reference tube. There was no statistical difference between different centrifugation durations and individual differences for AST, K and LD in LiH and/or Barricor (P > 0.05). There was a significant bias for LD between LiH and Barricor in terms of blood-sampling techniques (21.2% and 12.4%, respectively). Conclusions: Recently, the use of plasma has become prominent due to some of its advantages. In this study, plasma AST, K, LD, Glc and TP levels in Barricor were clinically different in comparison to serum. The results of additional tests showed that higher levels of LD in Barricor did not result from haemolysis, and they might be related to other factors including number of platelets, cellular fragility, or functional environment

Flow analysis in centrifugal compressor vaneless diffusers

348-354Flow computations have been performed in vaneless diffuser (diam, 1 m), in low speed backswept centrifugal compressor using finite volume method (FVM), coupled with Standard k-e turbulent model by solving on commercial package FLUENT assuming steady, incompressible flow condition with design flow-rate in Navier-Stokes equations. Mean velocities and pressure have been computed on eight planes through the diffuser. Flow field at diffuser entry clearly shows impeller jet-wake flow pattern and the blade wakes. Passage wake is located on shroud side of diffuser and mixes out slowly as flow moves through diffuser. On the other hand, blade wakes distort and mix out rapidly in diffuser. Contours of turbulent kinetic energy are also presented on each of the diffuser stations, from which regions of turbulent mixing are identified. Close agreement was observed between numerical simulation and experimental results

Effect of impeller-diffuser radial gap ratio in a centrifugal pump

203-213In this study, effects of flow behavior in a non-traditional centrifugal pump, whose diffuser was subjected to different radial gaps, were investigated numerically using multi-purpose FLUENT code. Flow was assumed to be three dimensional, viscous and incompressible. RNG with standard near-wall functions was used. Centrifugal pump had a five backward curved-bladed impeller running at 890 rpm and a nine vaned diffuser. Investigations for three different radial gaps (10%, 15%, 20%) have been carried out for 15 different volume flow rates. For present pump, 20% radial gap is found most suitable for pressure fluctuations

A multipurpose landmark for skull-base surgery: Henle's spine

Objective: To determine whether Henle's spine could be used as a reliable and multipurpose landmark for the other important structures of the skull base