Histological investigation of the effect of soybean (Glycine max) extracts on the collagen layer and estrogen receptors in the skin of female rats

OBJECTIVES: The purpose of this study was to analyze the effects of soybean extracts obtained using different extraction methods on the skin of female rats. METHOD: A total of 64 female Sprague-Dawley rats were divided into 8 equal groups. Various extracts were administered to the female rats by oral gavage for one month. The groups comprised carboxymethyl cellulose-free control, carboxymethyl cellulose-plus control, 100-mg/kg n-hexane extract, 200-mg/kg n-hexane extract, 100-mg/kg ethyl acetate extract, 200-mg/kg ethyl acetate extract, 100-mg/kg ethanol extract and 200-mg/kg ethanol extract groups. The thickness of the collagen layer and the number of estrogen receptor-positive cells were evaluated. RESULTS: All the extract-treated groups showed a statistically significant decrease in the number of estrogen receptor-positive cells compared with the control groups. Regarding the thickness of the collagen layer, only the 200-mg/kg ethyl acetate extract-treated group showed a significant increase compared with the control groups (

Montelukast jest skutecznym lekiem w zapobieganiu zespołowi hiperstymulacji jajników: badania eksperymentalne

Objectives: To determine the efficacy of montelukast in comparison with cabergoline in the prevention of ovarian hyperstimulation syndrome (OHSS) in rats. Material and methods: An experimental OHSS model was formed in 35 female Wistar rats. Rats (22 days old) were randomized into 5 groups, each containing 7 animals. The control group received no therapy; the mild OHSS group was administered pregnant mare serum gonadotropin (PMSG) 10 IU for 4 days, hCG 10 IU on the 5th day; the severe OHSS group received PMSG 10 IU for 4 days, hCG 30 IU on the 5th day. The montelukast group: received montelukast 10 mg/kg/day and the cabergoline group was administered cabergoline 100μg/kg/day via oral gavage for 6 days (days 22–27), in addition to those of severe OHSS. All groups were sacrificed on 28th day. Body weight, ovarian diameter and weight, vascular permeability, vascular endothelial growth factor (VEGF), semiquantitative VEGF receptor-1, and VEGF receptor-2 (VEGFR-2) immunohistochemistry were evaluated. Results: Ovarian diameter and VEGF expression were significantly lower in the montelukast and cabergoline groups than in the severe OHSS group. While montelukast was more effective in limiting vascular permeability in the severe OHSS, cabergoline was superior to montelukast with respect to the limiting effect on increased body weight and VEGFR-2 expression. Conclusions: The VEGF/VEGFR-2 interaction plays an important role in OHSS pathogenesis. Montelukast limits VEGF expression, and cabergoline reduces both VEGF and VEGFR-2 expressions; they are both effective therapies for the prevention of severe OHSS.Cel: Ocena skuteczności montelukastu w porównaniu z kabergoliną w zapobieganiu zespołowi hiperstymulacji jajników (OHSS) u szczurów. Materiał i metoda: Model doświadczalny OHSS stanowiło 35szczurów rasy Wistar, płci żeńskiej. Szczury (22 dniowe) podzielono na 5 grup, każda zawierająca 7 zwierząt. Grupa kontrolna nie otrzymała żadnej terapii. Grupa z łagodnym OHSS otrzymała gonadotropinę z surowicy ciężarnych klaczy (PMSG) w ilości 10IU przez 4 dni, hCG 10IU w 5 dniu, grupa z ciężkim OHSS otrzymała PMSG 10IU przez 4 dni, hCG 30IU w 5 dniu. Grupa z montelukastem otrzymała montelukast w dawce 10mg/kg/dzień a grupa z kabergoliną otrzymała kabergolinę 100μg/kg/dzień przez doustny zgłębnik przez 6 dni (dni 22-27). Wszystkie zwierzęta zabito w 28 dniu. Oceniono masę ciała, wymiar i wagę jajników, przepuszczalność naczyń, czynnik wzrostu śródbłonka naczyń (VEGF) oraz w immunohistochemii półilościowo receptor – 1 VEGF i receptor-2 VEGF. Wyniki: Wymiar jajnika oraz ekspresja VEGF były istotnie niższe w grupach z monelukastem i kabergoliną niż w grupie z ciężkim OHSS. Podczas gdy montelukast był bardziej skuteczny w ograniczaniu przepuszczalności śródbłonków w ciężkim OHSS, to kabergolina okazała się lepsza od montelukastu po uwzględnieniu ograniczającego efektu zwiększonej masy ciała i ekspresji VEGFR-2. Wnioski: Wzajemne oddziaływanie VEGF/VEGFR-2 odgrywa istotną role w patogenezie OHSS. Montelukast ogranicza ekspresję VEGF, a kabergolina zmniejsza zarówno ekspresję VEGF jak i VEGFR-2; obie terapie są skuteczne w zapobieganiu ciężkiemu OHSS

Effects of retinoic acid on compensatory lung growth

<p>Abstract</p> <p>Background</p> <p>We investigated the effect of Retinoic acid in the growth of contralateral lung after pneumonectomy.</p> <p>Methods</p> <p>Twentyone adult male Wistar albino rats from the same colony were used. They were divided into three groups (Group A, B and C). Group A undergone only left posterolateral thoracotomy. In Group B and C, the rats were subjected to left posterolateral thoracotomy and left pneumonectomy. In Group C, rats were given intraperitoneal Retinoic acid during the operation and continued to be given everyday postoperatively. Rats were sacrificed on the 10^thday and their total body, right lung weights and right lung volumes were measured.</p> <p>Results</p> <p>The volume and weight indices of the lung were found to be higher in Group C. In histopathological examination, there was a reduction in the mean number of alveoli in Group B and C. A significant rise in the mean dimension and average wall thickness of the alveolar structure were determined in Group C.</p> <p>Conclusion</p> <p>Retinoic acid contributes to the compensatory growth of the residual lung tissue.</p

Bazal hücreli karsinomlarda cyclin D1 ve Bcl-2 ekspresyonunun agresif davranışla ilişkisi

Bu tezin, veri tabanı üzerinden yayınlanma izni bulunmamaktadır. Yayınlanma izni olmayan tezlerin basılı kopyalarına Üniversite kütüphaneniz aracılığıyla (TÜBESS üzerinden) erişebilirsiniz.7. ÖZET Bu çalışmada agresiv davranışa etkisi bilinen gelişim paternine göre sınıflandırdığımız BHK olgularının immunohistokimyasal olarak gösterilebilen bcl-2 ve cyclin Dİ ekspresyonlarını karşılaştırmalı olarak değerlendirerek bu belirleyicilerin agresiv davranışı belirlemedeki katkılarını araştırmayı hedefledik. Ayrıca agresiv davranışı ve metasaz kapasitesi bilinen bazoskuamöz karsinom olgularını da bu belirleyiciler açısından diğer BHK tipleri ile karşılaştırmalı olarak değerlendirdik. Araştırmada 1999-2004 yılları arasında Ege üniversitesi Tıp Fakültesi Patoloji Anabilimdalı'na gelip, Bazal hücreli karsinom (78) ve bazoskuamöz karsmom(22) tanısı alan 92 hastaya ait 1 00 tümör kullanıldı. Bazal hücreli karsinomlar erkeklerde kadınlara göre daha sıktır, öncelikle baş-boyun bölgesi olmak üzere güneşe açık bölgelerde ve ileri yaşlarda görülür. Çalışmaya alınan olguların 53'ü erkek, 39'u kadındı, yaş ortalamaları, erkeklerde 63,88±1 3, 15, kadınlarda 62,46±1 3,3 1 bulundu. Bazoskuamöz karsinomlarda yaş ortalaması biraz daha yüksek ( 68.91 ) olmakla birlikte anlamlı bir fark saptanmadı. Tümörlerin 12'si kulak çevresinde olmak üzere 72'si baş-boyun, 8'i gövde yerleşimli idi. 20 tümörde yerleşim yeri belirtilmemişti. Gelişim paternine göre 23 olgu non agresiv grupta (nodüler BHK), 77 olgu agresiv grupta yer aldı. Agıesif gruba giren olguların 44'ü infiltratif BHK, 22si BSK, dokuzu da mikst tipte idi. Desmpolazi infiltratif BHK ve BSK olgularında görüldü. Cyclin Dİ değerleri; düşük (0-1+) ve yüksek (2-3+) grup olarak ele alındığında, non ıgı >_.-,! T (ıjouüleı tip) grup ile agresif tipler arasındaki fark istatistiksel olarak (p=0.040) anlamlı bulundu. Tüm BflK olgularında bcl-2 pozitifliği bulunmasına karşılık düşük dereceli (1+ ve 2+) po/.itilik ite agresiv davranış arasında anlamlı bir ilişki saptanmıştır. Yüksek pozitiflik ise (3+ ve 4+) nodüler tiplerde ortaya çıkmıştır. Mikronodüler BHK'lerde (2+ ve 3+) boyanma elde edilmiştir, bel 2 değerleri düşük (1-2+) ve yüksek (3-4+) olarakgruplandırrtdiğında non agresif (ııodlllcr tip) ile agresîf olarak a;nlaTnIı(P-0,00) fark bulunda, NOMİİ, *usuA n'munoİ!'* i^M'iF'it^'d i'u^ut^ --j|»t,),,M C,ıi ^^ '^! ^ s ^. ü rf.^ PÜI. u,;.>!, ;,, ;vl L, j,,,,,,,, _,.,M. '.,>!,.. i :t ı ' :,,..',, :,' _,, LU ıi',.ı ı.uUı 3

Coexistence of Sarcoidosis and Systemic Sclerosis

Sarcoidosis is a multisystem granulomatous disease characterized by hilar lymphadenopathy, involvement of internal organs, and diverse skin lesions. Systemic sclerosis is an autoimmune disease characterized by skin hardening and different internal organ fibrosis, including vascular abnormality. Immune response associated with Th-2 has been shown in the early and active stage of the disease. In this paper, we report coexistence of systemic sclerosis with sarcoidosis in a female patient presenting with granulomatous dermatitis, interstitial lung disease, and Raynaud's phenomenon complaints

Coexistence of Ankylosing Spondylitis and Löfgren’s Syndrome

A 46-year-old male patient diagnosed with ankylosing spondylitis presented to our polyclinic with complaints of pain, swelling, and limitation in joint mobility in both ankles and erythema nodosum skin lesions in both pretibial sites. The sacroiliac joint graphy and the MRI taken revealed active and chronic sacroiliitis. On the thorax CT, multiple mediastinal and hilar lymphadenopathies were reported. Mediastinoscopic excisional lymph node biopsy was taken and noncalcified granulomatous structures, lymphocytes, and histiocytes were determined on histopathological examination. The patients were diagnosed with ankylosing spondylitis, sarcoidosis, and Löfgren’s syndrome. NSAIDs, sulfasalazine, and low dose corticosteroid were started. Significant regression was seen in the patient’s subjective and laboratory assessments