Cereal and Pulse Crops with Improved Resistance to Pratylenchus thornei Are Needed to Maximize Wheat Production and Expand Crop Sequence Options

n the subtropical grain region of eastern Australia, two experiments were conducted, one initially with 2490 P. thornei/kg soil, the other with 8150 P. thornei/kg soil at 0–0.9 m soil depth. We determined the effect of P. thornei, residual from a weed-free fallow and pre-cropping with several cultivars each of barley (Hordeum vulgare), faba bean (Vicia faba), chickpea (Cicer arietinum), and wheat (Triticum aestivum) (Phase 1), on the growth of wheat cultivars with intolerance or tolerance to P. thornei (Phase 2). Pratylenchus thornei substantially increased after growing all cultivars of the Phase 1 faba bean, barley, and most cultivars of chickpea and wheat, and decreased after two moderately resistant wheat cultivars and the fallow treatment. The biomass of the Phase 2 tolerant cultivar ranged from 5070 to 6780 kg/ha and the intolerant cultivar 1020 to 4740 kg/ha. There was a negative linear relationship between P. thornei population densities and biomass of the Phase 2 intolerant cultivar but not of the tolerant cultivar. Growers are at risk of financial loss because they are restricted in their choice of crops to reduce damaging population densities of P. thornei. The development of resistant and tolerant crop genotypes can maximize production in P. thornei-affected farming systems

Solar Orbiter SWA Observations of Electron Strahl Properties Inside 1 AU

The Solar Wind Analyser (SWA) suite on Solar Orbiter includes an Electron Analyser System (SWA-EAS) which is capable of high temporal and angular resolution measurements of solar wind electrons in the energy range ∼1 eV to ∼5 keV. In this article we report early nominal phase observations of the suprathermal electron population at energies ≥70 eV (representative of the ’strahl’ population), and use a simple fitting routine and classification system to determine the characteristics of the distributions and determine the variations in their properties as a function of heliocentric distance and solar wind properties. We find that under our classification system a significant population of radially outward moving strahl beams is identifiable in the tested samples. These are seen in across solar wind speed regimes, but, consistent with earlier observations, are slightly more prevalent in high speed wind. These beams occur at all distances examined (∼0.43 to ∼1.0 AU), but do not show significant evolution with distance, suggesting a balance between focusing and scattering processes across the distance range covered. However, the data suggests that the beams broaden on average with increasing magnetic field strength and narrow on average with increasing solar wind speed. We also identify a small population, occurring in sporadic clusters, which have deficits in phase space density in the sunward moving part of the electron distribution. These clusters occur across the distance range sampled and show some variations in average properties with radial distance, suggesting they too are influenced by competing scattering and (de-)focusing processes. The implications for the origin and evolution of these electron populations derived from these new observations are explored

Assessment of High-Sensitivity C-Reactive Protein Levels as Diagnostic Discriminator of Maturity-Onset Diabetes of the Young Due to HNF1A Mutations

OBJECTIVE: Despite the clinical importance of an accurate diagnosis in individuals with monogenic forms of diabetes, restricted access to genetic testing leaves many patients with undiagnosed diabetes. Recently, common variation near the HNF1 homeobox A (HNF1A) gene was shown to influence C-reactive protein levels in healthy adults. We hypothesized that serum levels of high-sensitivity C-reactive protein (hs-CRP) could represent a clinically useful biomarker for the identification of HNF1A mutations causing maturity-onset diabetes of the young (MODY). RESEARCH DESIGN AND METHODS: Serum hs-CRP was measured in subjects with HNF1A-MODY (n = 31), autoimmune diabetes (n = 316), type 2 diabetes (n = 240), and glucokinase (GCK) MODY (n = 24) and in nondiabetic individuals (n = 198). The discriminative accuracy of hs-CRP was evaluated through receiver operating characteristic (ROC) curve analysis, and performance was compared with standard diagnostic criteria. Our primary analyses excluded approximately 11% of subjects in whom the single available hs-CRP measurement was >10 mg/l. RESULTS: Geometric mean (SD range) hs-CRP levels were significantly lower (

Testing Hydrodynamic Models of LMC X-4 with UV and X-ray Spectra

We compare the predictions of hydrodynamic models of the LMC X-4 X-ray binary system with observations of UV P Cygni lines with the GHRS and STIS spectrographs on the Hubble Space Telescope. The hydrodynamic model determines density and velocity fields of the stellar wind, wind-compressed disk, accretion stream, Keplerian accretion disk, and accretion disk wind. We use a Monte Carlo code to determine the UV P Cygni line profiles by simulating the radiative transfer of UV photons that originate on the star and are scattered in the wind. The qualitative orbital variation predicted is similar to that observed, although the model fails to reproduce the strong orbital asymmetry (the observed absorption is strongest for phi>0.5). The model predicts a mid-eclipse X-ray spectrum, due almost entirely to Compton scattering, with a factor 4 less flux than observed with ASCA. We discuss how the model may need to be altered to explain the spectral variability of the system.Comment: 11 figures, accepted by Ap

Non-field-aligned Proton Beams and Their Roles in the Growth of Fast Magnetosonic/Whistler Waves: Solar Orbiter Observations

The proton beam is an important population of the non-Maxwellian proton velocity distribution in the solar wind, but its role in wave activity remains unclear. In particular, the velocity vector of the proton beam and its influence on wave growth/damping have not been addressed before. Here we explore the origin and the associated particle dynamics of a kinetic wave event in the solar wind by analyzing measurements from Solar Orbiter and comparing them with theoretical predictions from linear Vlasov theory. We identify the waves as outward-propagating circularly polarized fast magnetosonic/whistler (FM/W) waves. The proton’s velocity distribution functions can destabilize FM/W waves. According to linear Vlasov theory, the velocity fluctuations of the core and the beam associated with FM/W waves render the original field-aligned background drift velocity non-field-aligned. This non-field-aligned drift velocity carrying the information of the velocity fluctuations of the core and the beam is responsible for the wave growth/damping. Specifically, for the FM/W waves we analyze, the non-field-aligned fluctuating velocity of the beam population is responsible for the growth of these unstable waves in the presence of a proton beam. In contrast, the core population plays the opposite role, partially suppressing the wave growth. Remarkably, the observed drift velocity vector between the core and the beam is not field aligned during an entire wave period. This result contrasts the traditional expectation that the proton beam is field aligned

RelA regulates CXCL1/CXCR2-dependent oncogene-induced senescence in murine Kras-driven pancreatic carcinogenesis

Tumor suppression that is mediated by oncogene-induced senescence (OIS) is considered to function as a safeguard during development of pancreatic ductal adenocarcinoma (PDAC). However, the mechanisms that regulate OIS in PDAC are poorly understood. Here, we have determined that nuclear RelA reinforces OIS to inhibit carcinogenesis in the Kras mouse model of PDAC. Inactivation of RelA accelerated pancreatic lesion formation in Kras mice by abrogating the senescence-associated secretory phenotype (SASP) gene transcription signature. Using genetic and pharmacological tools, we determined that RelA activation promotes OIS via elevation of the SASP factor CXCL1 (also known as KC), which activates CXCR2, during pancreatic carcinogenesis. In Kras mice, pancreas-specific inactivation of CXCR2 prevented OIS and was correlated with increased tumor proliferation and decreased survival. Moreover, reductions in CXCR2 levels were associated with advanced neoplastic lesions in tissue from human pancreatic specimens. Genetically disabling OIS in Kras mice caused RelA to promote tumor proliferation, suggesting a dual role for RelA signaling in pancreatic carcinogenesis. Taken together, our data suggest a pivotal role for RelA in regulating OIS in preneoplastic lesions and implicate the RelA/CXCL1/CXCR2 axis as an essential mechanism of tumor surveillance in PDAC

Low Frequency Variants in the Exons Only Encoding Isoform A of HNF1A Do Not Contribute to Susceptibility to Type 2 Diabetes

Background: There is considerable interest in the hypothesis that low frequency, intermediate penetrance variants contribute to the proportion of Type 2 Diabetes (T2D) susceptibility not attributable to the common variants uncovered through genome-wide association approaches. Genes previously implicated in monogenic and multifactorial forms of diabetes are obvious candidates in this respect. In this study, we focussed on exons 8-10 of the HNF1A gene since rare, penetrant mutations in these exons (which are only transcribed in selected HNF1A isoforms) are associated with a later age of diagnosis of Maturity onset diabetes of the young (MODY) than mutations in exons 1-7. The age of diagnosis in the subgroup of HNF1A-MODY individuals with exon 8-10 mutations overlaps with that of early multifactorial T2D, and we set out to test the hypothesis that these exons might also harbour low-frequency coding variants of intermediate penetrance that contribute to risk of multifactorial T2D. Methodology and principal findings: We performed targeted capillary resequencing of HNF1A exons 8-10 in 591 European T2D subjects enriched for genetic aetiology on the basis of an early age of diagnosis (≤ 45 years) and/or family history of T2D (≥ 1 affected sibling). PCR products were sequenced and compared to the published HNF1A sequence. We identified several variants (rs735396 [IVS9-24T>C], rs1169304 [IVS8+29T>C], c.1768+44C>T [IVS9+44C>T] and rd61953349 [c.1545G>A, p.T515T] but no novel non-synonymous coding variants were detected. Conclusions and significance: We conclude that low frequency, nonsynonymous coding variants in the terminal exons of HNF1A are unlikely to contribute to T2D-susceptibility in European samples. Nevertheless, the rationale for seeking low-frequency causal variants in genes known to contain rare, penetrant mutations remains strong and should motivate efforts to screen other genes in a similar fashion

Transdermal Blood Sampling for C-peptide Is a Minimally Invasive, Reliable Alternative to Venous Sampling in Children and Adults With Type 1 Diabetes

OBJECTIVE:C-peptide and islet autoantibodies are key type 1 diabetes biomarkers, typically requiring venous sampling, which limits their utility. We assessed transdermal capillary blood (TCB) collection as a practical alternative.RESEARCH DESIGN AND METHODS:Ninety-one individuals (71 with type 1 diabetes, 20 controls; individuals with type 1 diabetes: aged median 14.8 years [interquartile range (IQR) 9.1–17.1], diabetes duration 4.0 years [1.5–7.7]; controls: 42.2 years [38.0–52.1]) underwent contemporaneous venous and TCB sampling for measurement of plasma C-peptide. Participants with type 1 diabetes also provided venous serum and plasma, and TCB plasma for measurement of autoantibodies to glutamate decarboxylase, islet antigen-2, and zinc transporter 8. The ability of TCB plasma to detect significant endogenous insulin secretion (venous C-peptide ≥200 pmol/L) was compared along with agreement in levels, using Bland-Altman. Venous serum was compared with venous and TCB plasma for detection of autoantibodies, using established thresholds. Acceptability was assessed by age-appropriate questionnaire.RESULTS:Transdermal sampling took a mean of 2.35 min (SD 1.49). Median sample volume was 50 µL (IQR 40–50) with 3 of 91 (3.3%) failures, and 13 of 88 (14.7%) <35 µL. TCB C-peptide showed good agreement with venous plasma (mean venous ln[C-peptide] – TCB ln[C-peptide] = 0.008, 95% CI [−0.23, 0.29], with 100% [36 of 36] sensitivity/100% [50 of 50] specificity to detect venous C-peptide ≥200 pmol/L). Where venous serum in multiple autoantibody positive TCB plasma agreed in 22 of 32 (sensitivity 69%), comparative specificity was 35 of 36 (97%). TCB was preferred to venous sampling (type 1 diabetes: 63% vs. 7%; 30% undecided).CONCLUSIONS:Transdermal capillary testing for C-peptide is a sensitive, specific, and acceptable alternative to venous sampling; TCB sampling for islet autoantibodies needs further assessment