PHYTOSANITARY REGULATION AND AGRICULTURAL FLOWS: TOBACCO INPUTS AND CIGARETTES OUTPUTS

This paper examines the effects of the use of increasingly-popular phytosanitary regulations on production costs, and output and factor trade flows. The case addressed is that of the European regulation of maximum chemical residues in cigarettes manufactured with tobacco containing maleic hydrazide. The paper presents simulations of the effects of tightening the input/output market linkages and on the substitution away from the residue-contaminated U.S. input to residue-free non-U.S. inputs. This induced substitution results in higher costs, lower quantity supplied of the final product, and higher prices for U.S. cigarettes in Europe. Cross-price effects lead to higher quantities of EU cigarettes sold and a corresponding increase in the use of all inputs, including U.S. tobacco. When the U.S. tobacco price is allowed to fall, direct price effects stimulate the EU derived demand for U.S. tobacco. Although the regulation is protectionist in the output market, it leads to increased EU imports of the residue-contaminated input. When the price of U.S. tobacco adjusts, the regulation is actually antiprotective for EU growers. The regulation also indirectly influences production practices of U.S. tobacco growers and leads to lower levels of MH residues on U.S. leaf.Agricultural and Food Policy,

The Writing and Producing of Pecos Bill and the Indians, an Original Musical Comedy for Educational Theatre

The writing and producing of this musical comedy was a creative production thesis. The playwright also served as director and designer. The organization of the thesis is basically the organization of the project

Digital Circuit-Level Emulation of Transistor-Based Guitar Distortion Effects

The objective of this thesis was to model the Fuzz Face , a transistor-based guitar distortion effect, digitally at the circuit level, and explore how changes in the discrete analog components change the digital model. The circuit was first simulated using SPICE simulation software. Typically outputs and how they changed based on transistor gains were documented. A test circuit was then constructed in lab to determine true transistor gains. An analog Fuzz Face circuit was then constructed, and physical parameters were recorded. A digital model was then created using MATLAB. Capacitive filtering effects were found to be negligible in terms of the guitar signal and were not modeled. The transistors were modeled using the Ebers-Moll equations. A MATLAB algorithm was written to produce Fuzz Face type distortion given an input guitar signal. The algorithm used numerical techniques to solve the nonlinear equations and stored them in a look-up table. This table was used to process the input clips. The sound of the Fuzz Face was not perfectly modeled, but the equations were found to provide a reasonable approximation of the circuit. Further study is needed to determine a more complete modeling equation for the circuit.M.S.Committee Chair: Lanterman, Aaron; Committee Member: Hasler, Paul; Committee Member: Leach, Marshal

Asymmetric localisation of Miranda and its cargo proteins during neuroblast division requires the anaphase-promoting complex/cyclosome

Asymmetric cell divisions generate cell fate diversity during both invertebrate and vertebrate development. Drosophila neural progenitors or neuroblasts (NBs) each divide asymmetrically to produce a larger neuroblast and a smaller ganglion mother cell (GMC). The asymmetric localisation of neural cell fate determinants and their adapter proteins to the neuroblast cortex during mitosis facilitates their preferential segregation to the GMC upon cytokinesis. In this study we report a novel role for the anaphase-promoting complex/cyclosome (APC/C) during this process. Attenuation of APC/C activity disrupts the asymmetric localisation of the adapter protein Miranda and its associated cargo proteins Staufen, Prospero and Brat, but not other components of the asymmetric division machinery. We demonstrate that Miranda is ubiquitylated via its C-terminal domain; removal of this domain disrupts Miranda localisation and replacement of this domain with a ubiquitin moiety restores normal asymmetric Miranda localisation. Our results demonstrate that APC/C activity and ubiquitylation of Miranda are required for the asymmetric localisation of Miranda and its cargo proteins to the NB cortex

A mosaic genetic screen for novel mutations affecting Drosophila neuroblast divisions

BACKGROUND: The asymmetric segregation of determinants during cell division is a fundamental mechanism for generating cell fate diversity during development. In Drosophila, neural precursors (neuroblasts) divide in a stem cell-like manner generating a larger apical neuroblast and a smaller basal ganglion mother cell. The cell fate determinant Prospero and its adapter protein Miranda are asymmetrically localized to the basal cortex of the dividing neuroblast and segregated into the GMC upon cytokinesis. Previous screens to identify components of the asymmetric division machinery have concentrated on embryonic phenotypes. However, such screens are reaching saturation and are limited in that the maternal contribution of many genes can mask the effects of zygotic loss of function, and other approaches will be necessary to identify further genes involved in neuroblast asymmetric division. RESULTS: We have performed a genetic screen in the third instar larval brain using the basal localization of Miranda as a marker for neuroblast asymmetry. In addition to the examination of pupal lethal mutations, we have employed the MARCM (Mosaic Analysis with a Repressible Cell Marker) system to generate postembryonic clones of mutations with an early lethal phase. We have screened a total of 2,300 mutagenized chromosomes and isolated alleles affecting cell fate, the localization of basal determinants or the orientation of the mitotic spindle. We have also identified a number of complementation groups exhibiting defects in cell cycle progression and cytokinesis, including both novel genes and new alleles of known components of these processes. CONCLUSION: We have identified four mutations which affect the process of neuroblast asymmetric division. One of these, mapping to the imaginal discs arrested locus, suggests a novel role for the anaphase promoting complex/cyclosome (APC/C) in the targeting of determinants to the basal cortex. The identification and analysis of the remaining mutations will further advance our understanding of the process of asymmetric cell division. We have also isolated a number of mutations affecting cell division which will complement the functional genomics approaches to this process being employed by other laboratories. Taken together, these results demonstrate the value of mosaic screens in the identification of genes involved in neuroblast division

The forests of the United States : their use

Gerald W. Williams Collectio

Assimilation of Oil-Derived Elements by Oysters Due to the Deepwater Horizon Oil Spill

During and after the Deepwater Horizon Oil Spill (DWHOS), oysters (Crassostrea virginica) were exposed to oil and susceptible to incidental consumption of surface and subsurface oil materials. We determined the contribution of oil materials from the DWHOS to diet of oysters by comparing carbon (C) and nitrogen (N) stable isotope ratios in oyster shell to ratios in suspended particulate matter (SPM) and in fresh and weathered oil. Average δ13C and δ15N values in oyster shell (−21 ± 1‰ and 9−11‰, respectively) were consistent with consumption of naturally available SPM as opposed to values in oil (−27 ± 0.2‰, 1.6 ± 0.4‰). Stable isotope ratios in oyster adductor muscle were similar to shell for δ15N but not δ13C, suggesting either a recent shift in diet composition or differential assimilation of C between tissue types. We found no evidence of assimilation of oil-derived C and N and, therefore, no evidence of an oyster-based conduit to higher trophic levels. Trace elements in shell were inconclusive to corroborate oil exposure. These findings are not an indication that oysters were not exposed to oil; rather they imply oysters either did not consume oil-derived materials or consumed too little to be detectable compared to natural diet

Relationships among HIV infection, metabolic risk factors, and left ventricular structure and function

Our objective was to determine if the presence of metabolic complications (MC) conveyed an additional risk for left ventricular (LV) dysfunction in people with HIV. HIV(+) and HIV(−) men and women were categorized into four groups: (1) HIV(+) with MC (43±7 years, n=64), (2) HIV(+) without MC (42±7 years, n=59), (3) HIV(−) with MC (44±8 years, n=37), or (4) HIV(−) controls without MC (42±8 years, n=41). All participants underwent two-dimensional (2-D), Doppler, and tissue Doppler echocardiography. Overall, the prevalence of systolic dysfunction (15 vs. 4%, p=0.02) and LV hypertrophy (9 vs. 1%, p=0.03) was greater in HIV(+) than in HIV(−) participants. Participants with MC had a greater prevalence of LV hypertrophy (10% vs. 1%). Early mitral annular velocity during diastole was significantly (p<0.005) lower in groups with MC (HIV(+)/MC(+): 11.6±2.3, HIV(−)/MC(+): 12.0±2.3 vs. HIV(+)/MC(−): 12.4±2.3, HIV(−)/MC(−): 13.1±2.4 cm/s) and tended to be lower in groups with HIV (p=0.10). However, there was no interaction effect of HIV and MC for any systolic or diastolic variable. Regardless of HIV status, participants with MC had reduced LV diastolic function. Although both the presence of MC and HIV infection were associated with lower diastolic function, there was no additive negative effect of HIV on diastolic function beyond the effect of MC. Also, HIV was independently associated with lower systolic function. Clinical monitoring of LV function in individuals with metabolic risk factors, regardless of HIV status, is warranted

Avlusing med kaldt vann? – effektivitet og laksens velferd — sluttrapport FHF prosjekt 901488

Termisk behandling av oppdrettslaks med varmt vann blir hyppig brukt av næringen som avlusingsmetode. Behandlingen rapporteres å fjerne mobile lus effektivt, men kan også føre til dårligere fiskevelferd og økt dødelighet. Kaldt vann har potensiale som en ny termisk avlusingsmetode og kan også være bedre for laksens velferd. I dette pilotforsøket undersøkes effekten av kaldt vann på lusefjerning og fiskens velferd. Samlet sett var det avlusingseffekt på rundt 40 % ved de mest ekstreme behandlingene, men også en liten nedgang i velferdskår. Ytterligere studier er nødvendig før metoden kan tas i bruk. Særlig bør det undersøkes om kaldt vann gir bedre effekt i kombinasjon med mekanisk påvirkning, og hvordan fiskevelferd endres under kommersielle forhold.Avlusing med kaldt vann? – effektivitet og laksens velferd — sluttrapport FHF prosjekt 901488publishedVersio