La conception, l'élaboration et l'expérimentation d'un programme d'histoire nationale pour les élèves de onzième année

Québec Université Laval, Bibliothèque 201

Revue d'histoire du Bas Saint-Laurent, vol. 3 (3-4)

Éditorial : Un climat favorable -- Le transport du courrier par le Portage de Témiscouata -- Échec d'une grande artère dans les terres de l'intérieur. Le chemin Taché -- Chemin Kempt - Chemin Matapédia -- La venue du chemin de fer dans l'Est du Québec -- Coup d'oeil sur les moyens de transport d'autrefois -- Mentalités et réactions au changement : l'avènement de l'automobile dans le Bas Saint-Laurent -- La construction de la moto-neige : les premiers essais de monsieur J.-Adalbert Landry -- Un port de mer à Pointe-au-Père, Bic ou Rimouski? -- L'île Verte, ou la tyrannie des moyens de transport -- Trois générations de commerçants, trois générations de maires: la maison H.G. Lepage de Rimousk

A novel algorithm identifies stress-induced alterations in mitochondrial connectivity and inner membrane structure from confocal images

<div><p>Mitochondria exist as a highly interconnected network that is exquisitely sensitive to variations in nutrient availability, as well as a large array of cellular stresses. Changes in length and connectivity of this network, as well as alterations in the mitochondrial inner membrane (cristae), regulate cell fate by controlling metabolism, proliferation, differentiation, and cell death. Given the key roles of mitochondrial dynamics, the process by which mitochondria constantly fuse and fragment, the measure of mitochondrial length and connectivity provides crucial information on the health and activity of various cell populations. However, despite the importance of accurately measuring mitochondrial networks, the tools required to rapidly and accurately provide this information are lacking. Here, we developed a novel probabilistic approach to automatically measure mitochondrial length distribution and connectivity from confocal images. This method accurately identified mitochondrial changes caused by starvation or the inhibition of mitochondrial function. In addition, we successfully used the algorithm to measure changes in mitochondrial inner membrane/matrix occurring in response to Complex III inhibitors. As cristae rearrangements play a critical role in metabolic regulation and cell survival, this provides a rapid method to screen for proteins or compounds affecting this process. The algorithm will thus provide a robust tool to dissect the molecular mechanisms underlying the key roles of mitochondria in the regulation of cell fate.</p></div