Implementation Barriers of Industrial Symbiosis: A Systematic Review

Industrial Symbiosis is a regional inter-firm approach towards a more sustainable industry. However, the implementation of industrial symbiosis is hampered by a multitude and variety of barriers. Although, prior work has dealt with identifying barriers, an encompassing overview is missing to date. Therefore, this study provides a comprehensive overview and description of barriers of industrial symbiosis by reviewing the scientific literature. Barriers were identified and grouped through content analysis. In total, ca. 400 barriers for the implementation of industrial symbiosis were identified and categorized into three main categories and nine subcategories. The insights gained can be used to develop strategies and tools for further development and advancement of current industrial symbiosis practice to overcome existing barriers

Bringing Telemedicine Initiatives into Regular Care: Theoretical Underpinning for User-Centred Design Processes

Telemedicine is said to change the way care is delivered. Nevertheless, it still faces barriers to overcome the pilot stage and reach a majority of patients in regular care. Missing consideration of user-centred design processes is one major reason for this development as individuals are a key component for the technology’s success. Therefore, we aim to provide recommendations for a user-centred design process, which is, in turn, crucial to successfully implementing telemedicine innovations. To reach this aim, we identified individual-related barriers for telemedicine with an umbrella review. Furthermore, we related the barriers to the Unified Theory of Acceptance and Use of Technology (UTAUT2) proposed by Venkatesh and colleagues. A theoretical explanation helps to generate a broader understanding of what prevents individual acceptance of telemedicine innovations. The provided recommendations are supposed to support researchers and practitioners planning future telemedicine solutions

Changing seasonal temperature offers a window of opportunity for stricter climate policy

Environmental catastrophes, including the increased severity and frequency of climate extremes, can act as “windows of opportunities” that challenge citizens’ mental models and motivate them to engage in reflective processes, challenging their pre-conceived ideas. Less well understood is whether experiencing changing weather conditions, common in mid-latitudes, can have a similar effect and increase the citizens’ concerns about climate change and their willingness to accept more stringent climate policies. In this paper, we investigate the effects of changing seasonal temperature on the perceived seriousness of climate change and willingness to mitigate climate change. We use data from four yearly waves of a spatially explicit representative population survey in Germany and weather records from the postal code areas in which they live. To our knowledge, this study is the first analysis to link individual perceptions towards climate change and different mitigation options with seasonal temperature changes at specific locations in Europe. The analyzed perceptions were strongly influenced by socio-demographic characteristics and broader societal changes, as well as individual experiences of seasonal temperatures. The results show that experienced seasonal temperature change influences personal climate change concerns as well as the willingness to mitigate climate change, although with a weaker effect. The results indicate that it is the absolute temperature variation experienced that is important, rather than whether it is getting colder or warmer than usual. Considering the influences identified in this study can offer a window of opportunity for more stringent and targeted climate change policy

On the Road to Telemedicine Maturity: A Systematic Review and Classification of Telemedicine Maturity Models

Telemedicine, seen as a solution for growing healthcare problems, is still not reaching its full potential. Telemedicine pilots can result in high costs, without successfully increasing patients’ wellbeing as intended. Appropriate tools for scaling up telemedicine, like prescriptive maturity models, are needed. They can help people to assess the status quo and make progress with the scaling up process by presenting them with pre-defined improvement measures. Prior research has already led to the development of such tools, but an overview is still lacking as to which models fit which purpose and whether the measures presented are helpful and, if so, in what way. The aim of this research is to provide an overview and classification of existing prescriptive maturity models for telemedicine. A systematic literature review has been conducted and a classification scheme derived to assess the identified models. The resulting overview outlines a starting point for on-going research and presents a scheme for assessing existing models with regard to how fit they are for usage

Differences in Metabolite Composition of Aloe barbadensis Mill. Extracts Lead to Differential Effects on Human Blood T Cell Activity In Vitro

Aloe barbadensis Mill. (Aloe) is used for diverse therapeutic properties including immunomodulation. However, owing to the compositionally complex nature of Aloe, bioactive component(s) responsible for its beneficial properties, though thought to be attributed to polysaccharides (acemannan), remain unknown. We therefore aimed to determine the metabolite composition of various commercial Aloe extracts and assess their effects on human blood T cell activity in vitro. Peripheral blood mononuclear cells (PBMC) from healthy donors were stimulated polyclonally in presence or absence of various Aloe extracts. T cell phenotype and proliferation were investigated by flow cytometry. Aloe extracts were analyzed using targeted 1H-NMR spectroscopy for standard phytochemical quality characterization and untargeted gas chromatography mass spectrometry (GC-MS) for metabolite profiling. Aloe extracts differing in their standard phytochemical composition had varying effects on T cell activation, proliferation, apoptosis, and cell-death in vitro, although this was not related to the acemannan content. Furthermore, each Aloe extract had its own distinct metabolite profile, where extracts rich in diverse sugar and sugar-derivatives were associated with reduced T cell activity. Our results demonstrate that all commercial Aloe extracts are unique with distinct metabolite profiles, which lead to differential effects on T cell activity in vitro, independent of the acemannan content

PauLa - für mehr regionale Wertschöpfung: PauLa - Studie zu Potenzialen und Handlungsmöglichkeiten zur Erhöhung der Wertschöpfung in ausgewählten Sektoren der sächsischen Land- und Ernährungswirtschaft

Im Mittelpunkt der Studie PauLa stand die Frage, wie die regionale Wertschöpfung in der sächsischen Land- und Ernährungswirtschaft erhöht werden kann. Am Beispiel der Sektoren Milch, Getreide, Kartoffeln, Obst und Gemüse wurde deshalb die gesamte Lebensmittel-Wertschöpfungskette unter Berücksichtigung der vielfältigen Rahmenbedingungen betrachtet. Denkbare Zielszenarien wurden formuliert und Wertschöpfungspotenziale aufgezeigt. Im Ergebnis erfolgte die Zusammenstellung von sektorübergreifenden und sektorspezifischen Handlungsempfehlungen. Die Veröffentlichung richtet sich an die verschiedenen Akteure der Lebensmittel-Wertschöpfungskette. Redaktionsschluss: 15.05.202

Voluntary Wheel Running in Old C57BL/6 Mice Reduces Age-Related Inflammation in the Colon but Not in the Brain

Inflammation is considered a possible cause of cognitive decline during aging. This study investigates the influence of physical activity and social isolation in old mice on their cognitive functions and inflammation. The Barnes maze task was performed to assess spatial learning and memory in 3, 9, 15, 24, and 28 months old male C57BL/6 mice as well as following voluntary wheel running (VWR) and social isolation (SI) in 20 months old mice. Inflammatory gene expression was analyzed in hippocampal and colonic samples by qPCR. Cognitive decline occurs in mice between 15 and 24 months of age. VWR improved cognitive functions while SI had negative effects. Expression of inflammatory markers changed during aging in the hippocampus ( Il1a / Il6 / S100b / Iba1 / Adgre1 / Cd68 / Itgam ) and colon ( Tnf / Il6 / Il1ra / P2rx7 ). VWR attenuates inflammaging specifically in the colon ( Ifng / Il10 / Ccl2 / S100b / Iba1 ), while SI regulates intestinal Il1b and Gfap . Inflammatory markers in the hippocampus were not altered following VWR and SI. The main finding of our study is that both the hippocampus and colon exhibit an increase in inflammatory markers during aging, and that voluntary wheel running in old age exclusively attenuates intestinal inflammation. Based on the existence of the gut-brain axis, our results extend therapeutic approaches preserving cognitive functions in the elderly to the colon

Fecal luminal factors from patients with irritable bowel syndrome induce distinct gene expression of colonoids

Background: Alteration of the host-microbiota cross talk at the intestinal barrier may participate in the pathophysiology of irritable bowel syndrome (IBS). Therefore, we aimed to determine effects of fecal luminal factors from IBS patients on the colonic epithelium using colonoids. Methods: Colon-derived organoid monolayers, colonoids, generated from a healthy subject, underwent stimulation with fecal supernatants from healthy subjects and IBS patients with predominant diarrhea, phosphate-buffered saline (PBS), or lipopolysaccharide (LPS). Cytokines in cell cultures and fecal LPS were measured by ELISA and mRNA gene expression of monolayers was analyzed using Qiagen RT2 Profiler PCR Arrays. The fecal microbiota profile was determined by the GA-map™ dysbiosis test and the fecal metabolite profile was analyzed by untargeted liquid chromatography/mass spectrometry. Key results: Colonoid monolayers stimulated with fecal supernatants from healthy subjects (n\ua0=\ua07), PBS (n\ua0=\ua04) or LPS (n\ua0=\ua03) presented distinct gene expression profiles, with some overlap (R2Y\ua0=\ua00.70, Q2=\ua00.43). Addition of fecal supernatants from healthy subjects and IBS patients (n\ua0=\ua09) gave rise to different gene expression profiles of the colonoid monolayers (R2Y\ua0=\ua00.79, Q2=\ua00.64). Genes (n\ua0=\ua022) related to immune response (CD1D, TLR5) and barrier integrity (CLDN15, DSC2) contributed to the separation. Levels of proinflammatory cytokines in colonoid monolayer cultures were comparable when stimulated with fecal supernatants from either donor types. Fecal microbiota and metabolite profiles, but not LPS content, differed between the study groups. Conclusions: Fecal luminal factors from IBS patients induce a distinct colonic epithelial gene expression, potentially reflecting the disease pathophysiology. The culture of colonoids from healthy subjects with fecal supernatants from IBS patients may facilitate the exploration of IBS related intestinal micro-environmental and barrier interactions

A Distinct Faecal Microbiota and Metabolite Profile Linked to Bowel Habits in Patients with Irritable Bowel Syndrome

Patients with irritable bowel syndrome (IBS) are suggested to have an altered intestinal microenvironment. We therefore aimed to determine the intestinal microenvironment profile, based on faecal microbiota and metabolites, and the potential link to symptoms in IBS patients. The faecal microbiota was evaluated by the GA-map(TM) dysbiosis test, and tandem mass spectrometry (GC-MS/MS) was used for faecal metabolomic profiling in patients with IBS and healthy subjects. Symptom severity was assessed using the IBS Severity Scoring System and anxiety and depression were assessed using the Hospital Anxiety and Depression Scale. A principal component analysis based on faecal microbiota (n = 54) and metabolites (n = 155) showed a clear separation between IBS patients (n = 40) and healthy subjects (n = 18). Metabolites were the main driver of this separation. Additionally, the intestinal microenvironment profile differed between IBS patients with constipation (n = 15) and diarrhoea (n = 11), while no clustering was detected in subgroups of patients according to symptom severity or anxiety. Furthermore, ingenuity pathway analysis predicted amino acid metabolism and several cellular and molecular functions to be altered in IBS patients. Patients with IBS have a distinct faecal microbiota and metabolite profile linked to bowel habits. Intestinal microenvironment profiling, based on faecal microbiota and metabolites, may be considered as a future non-invasive diagnostic tool, alongside providing valuable insights into the pathophysiology of IBS

Fecal Luminal Factors from Patients with Gastrointestinal Diseases Alter Gene Expression Profiles in Caco-2 Cells and Colonoids

Previous in vitro studies have shown that the intestinal luminal content, including metabolites, possibly regulates epithelial layer responses to harmful stimuli and promotes disease. Therefore, we aimed to test the hypothesis that fecal supernatants from patients with colon cancer (CC), ulcerative colitis (UC) and irritable bowel syndrome (IBS) contain distinct metabolite profiles and establish their effects on Caco-2 cells and human-derived colon organoids (colonoids). The metabolite profiles of fecal supernatants were analyzed by liquid chromatography–mass spectrometry and distinguished patients with CC (n = 6), UC (n = 6), IBS (n = 6) and healthy subjects (n = 6). Caco-2 monolayers and human apical-out colonoids underwent stimulation with fecal supernatants from different patient groups and healthy subjects. Their addition did not impair monolayer integrity, as measured by transepithelial electrical resistance; however, fecal supernatants from different patient groups and healthy subjects altered the gene expression of Caco-2 monolayers, as well as colonoid cultures. In conclusion, the stimulation of Caco-2 cells and colonoids with fecal supernatants derived from CC, UC and IBS patients altered gene expression profiles, potentially reflecting the luminal microenvironment of the fecal sample donor. This experimental approach allows for investigating the crosstalk at the gut barrier and the effects of the gut microenvironment in the pathogenesis of intestinal diseases