19 research outputs found
Contrasting TiO2 compositions in early cenozoic mafic sills of the Faroe Islands : an example of basalt formation from distinct melting regimes
The Paleocene lava succession of the Faroe Islands Basalt Group (FIBG), which is a part of the North Atlantic Igneous Province (NAIP), is intruded by numerous basaltic sills. These can be grouped into three main categories according to their geochemical characteristics: A low-TiO2 sill category (TiO2 = 0.7-0.9), a relatively high-TiO2 sill category (TiO2 = 1.95-2.6) and an intermediate-TiO2 sill that displays major element compositions lying between the other two categories. Mantle normalised plots for the high-TiO2 and low-TiO2 sills display relatively uniform flat LREE trends and slightly steeper HREE slopes for high-TiO2 relative to low-TiO2 sills. The intermediate-TiO2 Morskranes Sill is LREE depleted. Mantle normalised trace elements of low-TiO2 sill samples define positive Eu and Sr anomalies, whereas high-TiO2 sill samples display negative anomalies for these same lements. Different Nb and Ta anomalies (positive versus negative) in many high-TiO2 versus low-TiO2 sill samples suggest various metasomatism of their sources prior to partial melting. The intermediate-TiO2 sill displays noticeably lower 87Sr/86Sr, 206Pb/204Pb and 208Pb/204Pb ratios relative to both the high-TiO2 and the low-TiO2 sill samples. Pb isotope compositions displayed by local contaminated basaltic lavas imply that some of these assimilated distinct crustal material from E Greenland or basement from NW Britain, while others probably assimilated only distinct E Greenland type of crustal material. A third crustal source of E Greenland or Rockall-type basement could be required in order to explain some of the range in lead isotopes displayed by the intermediate-TiO2 Morskranes Sill. Geochemical modelling suggest that Faroese high-TiO2 sills, could have formed by ~4 to 7.5% batch melting of moderately fertile lherzolites, while 16 to 21% batch melting fertile mantle sources could explain geochemical compositions of Faroese low-TiO2 sills. The intermediate-TiO2 sill samples could have formed by a range of 6 to 7% batch melting of a depleted mantle source, probably with a composition comparable to sources that gave rise to local low-TiO2 and intermediate-TiO2 host-rocks. Most Faroese sill samples probably developed outside the garnet stabilitry field and probably formed by batch melting of mantle materials comparable in composition to those reported for the sub-continental lithospheric mantle (SCLM) previously at depths of ⤠85 km. Relative enrichments in LREE (and LILE in general), and their varying Nb and Ta anomalies point to sources affected by metasomatism
Assessment of leachable elements in volcanic ashfall : a review and evaluation of a standardized protocol for ash hazard characterization.
Volcanic ash presents a widespread and common hazard during and after eruptions. Complex interactions between solid ash surfaces and volcanic gases lead to the formation of soluble salts that may be mobilized in aqueous environments. A variety of stakeholders may be concerned about the effects of ash on human and animal health, drinking water supplies, crops, soils and surface runoff. As part of the immediate emergency response, rapid dissemination of information regarding potentially hazardous concentrations of soluble species is critical. However, substantial variability in the methods used to characterize leachable elements makes it challenging to compare datasets and eruption impacts. To address these challenges, the International Volcanic Health Hazard Network (www.ivhhn.org) organized a two-day workshop to define appropriate methods for hazard assessment. The outcome of this workshop was a âconsensus protocolâ for analysis of volcanic ash samples for rapid assessment of hazards from leachable elements, which was subsequently ratified by leading volcanological organizations. The purpose of this protocol is to recommend clear, standard and reliable methods applicable to a range of purposes during eruption response, such as assessing impacts on drinking-water supplies and ingestion hazards to livestock, and also applicable to research purposes. Where possible, it is intended that the methods make use of commonly available equipment and require little training. To evaluate method transferability, an interlaboratory comparison exercise was organized among six laboratories worldwide. Each laboratory received a split of pristine ash, and independently analyzed it according to the protocol for a wide range of elements. Collated results indicate good repeatability and reproducibility for most elements, thus indicating that the development of this protocol is a useful step towards providing standardized and reliable methods for ash hazard characterization. In this article, we review recent ash leachate studies, report the outcomes of the comparison exercise and present a revised and updated protocol based on the experiences and recommendations of the exercise participants. The adoption of standardized methods will improve and facilitate the comparability of results among studies and enable the ongoing development of a global database of leachate information relevant for informing volcanic health hazards assessment
BoletĂn Oficial de la Provincia de Oviedo: NĂşmero 154 - 1962 julio 7
Prostate cancer (PCa) is an increasing health issue worldwide. It is of a particular concern in New Zealand, which has one of the highest incidences of PCa globally. Broadly, PCa is composed of two main forms; latent organ-confined disease and aggressive metastatic. Treatment options for the aggressive form are limited to androgen deprivation therapy, which is only effective for a short period of time. Paired with this, the current diagnostic options available are unable to distinguish between benign prostate disease and PCa. This warrants research into more robust diagnostic and therapeutic options.
Activins are members of the TGF-β superfamily and a clear association between activins and PCa has been described. Of particular importance is activin A, which has the potential to impede cancer development by promoting apoptosis and inhibiting cell proliferation. Given the potency of activin A, tight regulation is critical and one level is provided in the form of antagonists, such as follistatin. In previous years it has become clear that other factors are crucial in the regulation of cellular processes, particularly non-coding RNAs, such as microRNAs (miRNAs). MiRNAs are ~18-23 nucleotides in length and function to negatively regulate gene expression at the level of translation. While miRNAs have been shown to be important in physiological processes they have also been implicated in disease, particularly cancer. The effects of activin A on human embryonic stem cells has been investigated previously, however, the impact of activin A on miRNAs in PCa has not. This therefore represented a significant void in the literature. Hence, the overarching aim of this thesis was to investigate the effect of activin A on miRNA expression in prostate cell lines, particularly LNCaP cells, which are highly sensitive to activin A.
These data herein, demonstrate the potential of activin A to modulate miRNA expression in LNCaP cells. Initially, RT-qPCR showed that 9 miRNAs demonstrated significant alterations following activin A treatment. Utilising miRPath enrichment software it was evident that these miRNAs were targeting genes contained in both canonical and non-canonical activin A signalling pathways, including, CDKN1B, PCNA, and MKI67, whose inverse expression correlated with the expression of numerous activin A-mediated miRNAs. Subsequently, following treatment with activin A and follistatin, NanoString technology revealed that 5 miRNAs significantly altered by activin A were antagonised by follistatin. Furthermore, NanoString PanCancer analysis demonstrated that MYC was highlighted in of 6 out of 13 canonical cancer pathways following activin A treatment, of which MYC was shown to contain binding sites for activin A-mediated miRNAs. MYC, therefore could be playing a central role following activin A treatment. Next-generation sequencing libraries for activin A and media control were generated for LNCaP cells and screened for the presence of novel miRNAs using miRDeep* software and identified a potentially novel miRNA which was modulated by activin A.
In conclusion, for the first time, these data demonstrate a relationship between activin A, miRNA expression and downstream target genes in PCa. This thesis forms a foundation for further investigation into activin A expression levels in conjunction with the expression of miRNAs and the genes they target. These findings may provide potential targets for the diagnosis and treatment of PCa
Yigitbas Erdinc; Winchester John A.; Ottley Christopher J. (2008). The geochemistry and setting of the Demirci paragneisses of the Sunnice Massif, NW Turkey.
Proterozoic ophiolites and mafic-ultramafic complexes marginal to the Istanbul Block: An exotic terrane of Avalonian affinity in NW Turkey
Among the Proterozoic inliers in the Istanbul Block, the lowest structural levels are exposed in the Sunnice Massif, north of Bolu. Amphibolite-facies mafic and subordinate ultramafic rocks of the Cele meta-ophiolite underlie the greenschist-facies Ediacaran calc-alkaline Yellice metavolcanics, which are intruded by the similar to 565-576 Ma Dirgine granitoids. Homblende gneisses of the Cele meta-ophiolite comprise island arc metatholeiites and transitional to calc-alkaline metabasalts which, together with minor serpentinite are disposed in a broadly antiformal structure. The meta-ophiolitic rocks are therefore the oldest ophiolites in NW Turkey, and are themselves thrust on to a putative pre-existing continental margin, now represented by the metasedimentary migmatites of the Demirci gneisses, which may thus be the oldest rocks of the complex. The Istanbul Block is an exotic terrane. Unlike other western Turkish terranes, it lacks Variscan metamorphism: its different provenance, indicated by its geological record, faunal affinities, and inherited mid-Proterozoic isotopic dates, suggests a former link with Avalonian basement in England, NW Europe and the Maritime Provinces of Canada. Hence, together with other terranes now situated further east than the Avalonian terranes of NW Europe, the Istanbul Block may represent the easternmost extremities of Avalonia, which were detached during its end-Ordovician collision with the Bruno-Silesian Promontory on the SW margin of Baltica. Subsequent migration of the Istanbul Block to its present location occurred by eastward displacement by sinistral transpression along the southern margin of Baltica to a point east of the Dobrogea and south of the Scythian Platform, followed by collision with the Sakarya Block in the Mesozoic and Late Cretaceous southward displacement with the opening of the Black Sea basin
Bozkurt Erdin; Winchester John A.; Yigitbas Erdinc and Ottley Christopher J.. Proterozoic ophiolites and mafic-ultramafic complexes marginal to the Istanbul Block: An exotic terrane of Avalonian affinity in NW Turkey.
Compartmentalisation and groundwaterâsurface water interactions in a prospective shale gas basin: Assessment using variance analysis and multivariate statistics on water quality data
An environmental concern with hydraulic fracturing for shale gas is the risk of groundwater and surface water contamination. Assessing this risk partly involves the identification and understanding of groundwaterâsurface water interactions because potentially contaminating fluids could move from one water body to the other along hydraulic pathways. In this study we use water quality data from a prospective shale gas basin to determine: if surface water sampling could identify groundwater compartmentalisation by lowâpermeability faults; and if surface waters interact with groundwater in underlying bedrock formations, thereby indicating hydraulic pathways. Variance analysis showed that bedrock geology was a significant factor influencing surface water quality, indicating regionalâscale groundwaterâsurface water interactions despite the presence of an overlying regionâwide layer of superficial deposits averaging 30â40âm thickness. We propose that surface waters interact with a weathered bedrock layer through the complex distribution of glaciofluvial sands and gravels. Principal component analysis showed that surface water compositions were constrained within groundwater endâmember compositions. Surface water quality data showed no relationship with groundwater compartmentalisation known to be caused by a major basin fault. Therefore, there was no chemical evidence to suggest that deeper groundwater in this particular area of the prospective basin was reaching the surface in response to compartmentalisation. Consequently, in this case compartmentalisation does not appear to increase the risk of frackingârelated contaminants reaching surface waters, although this may differ under different hydrogeological scenarios
Lilypad aggregation: localised self-assembly and metal sequestration at a liquid-vapour interface
Spatially resolved soft materials, such as vesicles and microgels, have shown promise as selective adsorbents and microscale reaction vessels. However, spatiotemporal control of aggregation can be difficult to achieve. In this study, nickel(II) chloride and a dipyridyl oligo(urea) ligand were combined in a vapour-diffusion setup to produce a localised spheroidal aggregate at the liquidâvapour interface. This aggregate forms via the self-assembly and fusion of monodisperse colloids and grows until its weight is no longer counterbalanced by surface tension. A simple physical model reveals that this process, termed lilypad aggregation, is possible only for surface energies that favour neither bulk aggregation nor the growth of an interfacial film. These surface energies dictate the final size and shape of the aggregate and may be estimated through visual monitoring of its changing morphology. Lilypad aggregates sequester metal from the surrounding sol and can be collected manually from the surface of the liquid
Cellular and molecular mechanisms for the synergistic cytotoxicity elicited by oxaliplatin and pemetrexed in colon cancer cell lines
Purpose: Oxaliplatin effect in the treatment of colorectal cancer is improved upon combination with thymidylate synthase (TS) inhibitors. Pemetrexed is polyglutamated by the folylpolyglutamate synthase (FPGS) and blocks folate metabolism and DNA synthesis by inhibiting TS, dihydrofolate reductase (DHFR) and glycinamide ribonucleotide formyltransferase (GARFT). The present study evaluates the pharmacological interaction between oxaliplatin and pemetrexed in colorectal cancer cells. Methods: Human HT29, WiDr, SW620 and LS174T cells were treated with oxaliplatin and pemetrexed. Drug interaction was studied using the combination index method, while cell cycle was investigated with flow cytometry. The effects of drugs on Akt phosphorylation and apoptosis were studied with ELISA and fluorescence microscopy, respectively. RT-PCR analysis was performed to assess whether drugs modulated the expression of pemetrexed targets and of genes involved in DNA repair (ERCC1 and ERCC2). Finally, platinumâDNA adduct levels were detected by ultra-sensitive multi-collector inductively coupled plasma mass spectrometry (ICP-MS). Results: A dose-dependent inhibition of cell growth was observed after drug exposure, while a synergistic interaction was detected preferentially with sequential combinations. Oxaliplatin enhanced cellular population in the S-phase. Drug combinations increased apoptotic indices with respect to single agents, and both drugs inhibited Akt phosphorylation. RT-PCR analysis showed a correlation between the FPGS/(TS Ă DHFR Ă GARFT) ratio and pemetrexed sensitivity, as well as a downregulation of ERCC1, ERCC2, TS, DHFR and GARFT after drug exposure. In addition, pretreatment with pemetrexed resulted in an increase of oxaliplatinâDNA adducts. Conclusion: These data demonstrate that oxaliplatin and pemetrexed synergistically interact against colon cancer cells, through modulation of cell cycle, inhibition of Akt phosphorylation, induction of apoptosis and modulation of gene expression
Substituted β-cyclodextrin and calix[4]arene as encapsulatory vehicles for platinum(II)-based DNA intercalators
The encapsulation of three platinum(II)-based anticancer complexes, [(5,6-dimethyl-1,10-phenanthroline)(1S,2Sdiaminocyclohexane) platinum(II)]2+ (56MESS), [(5,6-dimethyl-1,10-phenanthroline)(1R,2R-diaminocyclohexane)platinum( II)]2+ (56MERR), and [(5,6-dimethyl-1,10-phenanthroline)(ethylenediamine)platinum(II)]2+ (56MEEN), with carboxylated-β-cyclodextrin (c-β-CD) and p-sulfonatocalix[4]arene (s-CX[4]) has been examined by one- and twodimensional 1H nuclear magnetic resonance (NMR) spectroscopy, pulsed gradient spin-echo NMR, ultraviolet spectrophotometry, glutathione degradation experiments, and growth inhibition assays. Titration of any of the three metal complexes with c-β-CD resulted in 1:1 encapsulation complexes with the cyclodextrin located over the intercalating ligand of the metal complexes, with a binding constant of 104-105 M-1. In addition to binding over the phenanthroline ligand of 56MEEN, c-β-CD was also found to portal bind to the ethylenediamine ligand, with fast exchange kinetics on the NMR timescale between the two binding sites. In contrast, the three metal complexes all formed 2:2 inclusion complexes with s-CX[4] where the two metal complexes stacked in a head-to-tail configuration and were capped by the s-CX[4] molecules. Interestingly, the 56MEEN-s-CX[4] complex appeared to undergo a thermodynamically controlled rearrangement to a less soluble complex over time. Encapsulation of the metal complexes in either c-β-CD or s-CX[4] significantly decreased the metal complexesâ rate of diffusion, consistent with the formation of larger particle volumes. Encapsulation of 56MESS within s-CX[4] or c-β-CD protected the metal complex from degradation by reduced L-glutathione, with a reaction half-life greater than 9 days. In vitro growth inhibition assays using the LoVo human colorectal cancer cell line showed no significant change in the cytotoxicity of 56MESS when encapsulated by either s-CX[4] or c-β-CD