Potential distribution and biosecurity risks from three economically important plant - parasitic nematodes

The plant-parasitic nematodes (PPNs), Heterodera zeae, Hirschmanniella oryzae and Meloidogyne graminicola, are economically important pests of major grain crops. Each of these species is still absent from the majority of countries, so there is great concern about their spread. However, the areas particularly at risk of invasion and the potential global distributions of these nematode pests are yet to be identified. This paper identifies these areas, together with the main factors that could facilitate establishment in new areas using CLIMEX models. Parameterised using published studies of species phenology and global species distributions, the models correctly identified the areas invaded recently by M. graminicola and H. zeae. The vast majority of the areas of the world under wheat, rice and maize were identified as suitable for the nematodes. The nematodes are not yet established in many of these areas, suggesting that biosecurity measures may be particularly warranted to prevent the spread of these species to the new suitable areas identified. Intensive cropping systems under irrigation were identified as being at much greater risk than rain-fed cropping systems. The potential detrimental effects from introduction and local spread of PPNs in irrigation water could undermine suggested potential gains from using irrigation to increase world food production and meet increasing demand. These results mean that biosecurity measures for PPNs are justified

Intestinal Diffuse Large B-Cell Lymphoma in a Patient with Systemic Lupus Erythematosus

A 44-year-old Japanese woman with systemic lupus erythematosus (SLE) presented to our hospital with abdominal pain. Radiological and endoscopic examinations led to the diagnosis of diffuse large B-cell lymphoma of the jejunum, which was subsequently resected. Patients with SLE reportedly have an increased risk of non-Hodgkin lymphoma, as demonstrated by our patient. Hence, lymphoma should be considered in the differential diagnosis of neoplastic lesions emerging in SLE patients. In addition, flow cytometry using endoscopically biopsied fragments is useful for the immediate diagnosis of lymphoma, leading to timely and accurate preoperative staging

Improving climate suitability for Bemisia tabaci in East Africa is correlated with increased prevalence of whiteflies and cassava diseases

Projected climate changes are thought to promote emerging infectious diseases, though to date, evidence linking climate changes and such diseases in plants has not been available. Cassava is perhaps the most important crop in Africa for smallholder farmers. Since the late 1990's there have been reports from East and Central Africa of pandemics of begomoviruses in cassava linked to high abundances of whitefly species within the Bemisia tabaci complex. We used CLIMEX, a process-oriented climatic niche model, to explore if this pandemic was linked to recent historical climatic changes. The climatic niche model was corroborated with independent observed field abundance of B. tabaci in Uganda over a 13-year time-series, and with the probability of occurrence of B. tabaci over 2 years across the African study area. Throughout a 39-year climate time-series spanning the period during which the pandemics emerged, the modelled climatic conditions for B. tabaci improved significantly in the areas where the pandemics had been reported and were constant or decreased elsewhere. This is the first reported case where observed historical climate changes have been attributed to the increase in abundance of an insect pest, contributing to a crop disease pandemic

An operative case of hepatic pseudolymphoma difficult to differentiate from primary hepatic marginal zone B-cell lymphoma of mucosa-associated lymphoid tissue

Hepatic pseudolymphoma (HPL) and primary hepatic marginal zone B cell lymphoma of mucosa-associated lymphoid tissue (MALT lymphoma) are rare diseases and the differential diagnosis between these two entities is sometimes difficult. We herein report a 56-year-old Japanese woman who was pointed out to have a space occupying lesion in the left lateral segment of the liver. Hepatitis viral-associated antigen/antibody was negative and liver function tests including lactic dehydrogenase, peripheral blood count, tumor markers and soluble interleukin-2 receptor were all within normal limit. Imaging study using computed tomography and magnetic resonance imaging were not typical for hepatocellular carcinoma, cholangiocarcinoma, or other metastatic cancer. Fluorodeoxyglucose-positron emission tomography examination integrated with computed tomography scanning showed high standardized uptake value in the solitary lesion in the liver. Under a diagnosis of primary liver neoplasm, laparoscopic-assisted lateral segmentectomy was performed. Liver tumor of maximal 1.0 cm in diameter was consisted of aggregation of lymphocytes of predominantly B-cell, containing multiple lymphocyte follicles positive for CD10 and bcl-2, consistent with a diagnosis of HPL rather than MALT lymphoma, although a definitive differentiation was pending. The background liver showed non-alcoholic fatty liver disease/early non-alcoholic steatohepatitis. The patient is currently doing well with no sign of relapse 13 months after the surgery. Since the accurate diagnosis is difficult, laparoscopic approach would provide a reasonable procedure of diagnostic and therapeutic advantage with minimal invasiveness for patients. Considering that the real nature of this entity remains unclear, vigilant follow-up of patient is essential

LECT2 functions as a hepatokine that links obesity to skeletal muscle insulin resistance

Recent articles have reported an association between fatty liver disease and systemic insulin resistance in humans, but the causal relationship remains unclear. The liver may contribute to muscle insulin resistance by releasing secretory proteins called hepatokines. Here we demonstrate that leukocyte cell-derived chemotaxin 2 (LECT2), an energy-sensing hepatokine, is a link between obesity and skeletal muscle insulin resistance. Circulating LECT2 positively correlated with the severity of both obesity and insulin resistance in humans. LECT2 expression was negatively regulated by starvation-sensing kinase adenosine monophosphate-activated protein kinase in H4IIEC hepatocytes. Genetic deletion of LECT2 in mice increased insulin sensitivity in the skeletal muscle. Treatment with recombinant LECT2 protein impaired insulin signaling via phosphorylation of Jun NH2-terminal kinase in C2C12 myocytes. These results demonstrate the involvement of LECT2 in glucose metabolism and suggest that LECT2 may be a therapeutic target for obesity-associated insulin resistance. © 2014 by the American Diabetes Association

The whole blood transcriptional regulation landscape in 465 COVID-19 infected samples from Japan COVID-19 Task Force

「コロナ制圧タスクフォース」COVID-19患者由来の血液細胞における遺伝子発現の網羅的解析 --重症度に応じた遺伝子発現の変化には、ヒトゲノム配列の個人差が影響する--. 京都大学プレスリリース. 2022-08-23.Coronavirus disease 2019 (COVID-19) is a recently-emerged infectious disease that has caused millions of deaths, where comprehensive understanding of disease mechanisms is still unestablished. In particular, studies of gene expression dynamics and regulation landscape in COVID-19 infected individuals are limited. Here, we report on a thorough analysis of whole blood RNA-seq data from 465 genotyped samples from the Japan COVID-19 Task Force, including 359 severe and 106 non-severe COVID-19 cases. We discover 1169 putative causal expression quantitative trait loci (eQTLs) including 34 possible colocalizations with biobank fine-mapping results of hematopoietic traits in a Japanese population, 1549 putative causal splice QTLs (sQTLs; e.g. two independent sQTLs at TOR1AIP1), as well as biologically interpretable trans-eQTL examples (e.g., REST and STING1), all fine-mapped at single variant resolution. We perform differential gene expression analysis to elucidate 198 genes with increased expression in severe COVID-19 cases and enriched for innate immune-related functions. Finally, we evaluate the limited but non-zero effect of COVID-19 phenotype on eQTL discovery, and highlight the presence of COVID-19 severity-interaction eQTLs (ieQTLs; e.g., CLEC4C and MYBL2). Our study provides a comprehensive catalog of whole blood regulatory variants in Japanese, as well as a reference for transcriptional landscapes in response to COVID-19 infection

DOCK2 is involved in the host genetics and biology of severe COVID-19

「コロナ制圧タスクフォース」COVID-19疾患感受性遺伝子DOCK2の重症化機序を解明 --アジア最大のバイオレポジトリーでCOVID-19の治療標的を発見--. 京都大学プレスリリース. 2022-08-10.Identifying the host genetic factors underlying severe COVID-19 is an emerging challenge. Here we conducted a genome-wide association study (GWAS) involving 2, 393 cases of COVID-19 in a cohort of Japanese individuals collected during the initial waves of the pandemic, with 3, 289 unaffected controls. We identified a variant on chromosome 5 at 5q35 (rs60200309-A), close to the dedicator of cytokinesis 2 gene (DOCK2), which was associated with severe COVID-19 in patients less than 65 years of age. This risk allele was prevalent in East Asian individuals but rare in Europeans, highlighting the value of genome-wide association studies in non-European populations. RNA-sequencing analysis of 473 bulk peripheral blood samples identified decreased expression of DOCK2 associated with the risk allele in these younger patients. DOCK2 expression was suppressed in patients with severe cases of COVID-19. Single-cell RNA-sequencing analysis (n = 61 individuals) identified cell-type-specific downregulation of DOCK2 and a COVID-19-specific decreasing effect of the risk allele on DOCK2 expression in non-classical monocytes. Immunohistochemistry of lung specimens from patients with severe COVID-19 pneumonia showed suppressed DOCK2 expression. Moreover, inhibition of DOCK2 function with CPYPP increased the severity of pneumonia in a Syrian hamster model of SARS-CoV-2 infection, characterized by weight loss, lung oedema, enhanced viral loads, impaired macrophage recruitment and dysregulated type I interferon responses. We conclude that DOCK2 has an important role in the host immune response to SARS-CoV-2 infection and the development of severe COVID-19, and could be further explored as a potential biomarker and/or therapeutic target

Queries on the Birth of Poet Ishikawa Takuboku

近年來日本近代文學研究受到文化研究（Culture Studies）的影響，文學作品或作家主體本身之研究，呈現被驅至幕後的傾向。本稿主要考察明治36年12月《明星》雜誌中，詩人石川啄木（1886─1912）以雅號「啄木」初次登場之經過。以往的說法是，雅號「啄木」為《明星》主編者與謝野鐵幹命名之；而現在則是以岩城之德所提出的啄木自我命名說為定論。不過竊以為「啄木」之雅號是鐵幹和啄木兩人共同合作命名的。本稿企圖藉由重新探討雅號命名之問題，考察「作品」和「作家」的關係，進而反省此考察在文學研究中所具有之意義。The recent trend of the so-called cultural research by the Japanese contemporary literature research is on the detailed probing in the literature creation or on the writer’s background. The focus of this paper is to probe into the poet who has an elegant name, “Takuboku”, and has his premier appearance in the 1903 December edition of the “Myojo” -Ishikawa Takuboku (1886-1912). Some say name “Takuboku” was named by the master of the “Myojo”- and Yosono Tekkan while Iwaki Yukinori believed it was Takuboku whom had given himself of such name. As for me, I would think it was the name under the collaboration of Tekkan and Takuboku. But the question I would like to raise is the meaning of probing into the relationships between the “composition” and the “writer” in the research of literature through the discussion of elegant names

Queries on the Birth of Poet Ishikawa Takuboku

近年來日本近代文學研究受到文化研究（Culture Studies）的影響，文學作品或作家主體本身之研究，呈現被驅至幕後的傾向。本稿主要考察明治36年12月《明星》雜誌中，詩人石川啄木（1886─1912）以雅號「啄木」初次登場之經過。以往的說法是，雅號「啄木」為《明星》主編者與謝野鐵幹命名之；而現在則是以岩城之德所提出的啄木自我命名說為定論。不過竊以為「啄木」之雅號是鐵幹和啄木兩人共同合作命名的。本稿企圖藉由重新探討雅號命名之問題，考察「作品」和「作家」的關係，進而反省此考察在文學研究中所具有之意義。The recent trend of the so-called cultural research by the Japanese contemporary literature research is on the detailed probing in the literature creation or on the writer’s background. The focus of this paper is to probe into the poet who has an elegant name, “Takuboku”, and has his premier appearance in the 1903 December edition of the “Myojo” -Ishikawa Takuboku (1886-1912). Some say name “Takuboku” was named by the master of the “Myojo”- and Yosono Tekkan while Iwaki Yukinori believed it was Takuboku whom had given himself of such name. As for me, I would think it was the name under the collaboration of Tekkan and Takuboku. But the question I would like to raise is the meaning of probing into the relationships between the “composition” and the “writer” in the research of literature through the discussion of elegant names

The potential distribution of cassava mealybug (Phenacoccus manihoti), a threat to food security for the poor

The cassava mealybug is a clear and present threat to the food security and livelihoods of some of the world's most impoverished citizens. Niche models, such as CLIMEX, are useful tools to indicate where and when such threats may extend, and can assist with planning for biosecurity and the management of pest invasions. They can also contribute to bioeconomic analyses that underpin the allocation of resources to alleviate poverty. Because species can invade and establish in areas with climates that are different from those that are found in their native range, it is essential to define robust range-limiting mechanisms in niche models. To avoid spurious results when applied to novel climates, it is necessary to employ crossvalidation techniques spanning different knowledge domains (e.g., distribution data, experimental results, phenological observations). We build upon and update a CLIMEX niche model by Parsa et al. (PloS ONE 7: e47675), correcting inconsistent parameters and re-fitting it based on a careful examination of geographical distribution data and relevant literature. Further, we consider the role of irrigation, the known distribution of cassava production and a targeted review of satellite imagery to refine, validate and interpret our model and results. In so doing, we bring new insights into the potential spread of this invasive insect, enabling us to identify potential bio-security threats and biological control opportunities. The fit of the revised model is improved, particularly in relation to the wet and dry limits to establishment, and the parameter values are biologically plausible and accord with published scientific literature