In situ immobilization of cadmium and zinc in contaminated soils : fiction or fixation?

Keywords: beringite, cadmium, DOC, DOM, earthworms, immobilization, leaching, lime, manganese oxides, metal binding, metal uptake, organic matter partitioning, pH, soil contamination, remediation, sorption, Swiss chard, zeolites, zinc.It is generally assumed that a decrease in metal concentration in the soil solution reduces metal leaching, and metal uptake by and toxicity to plants and soil organisms. In situ immobilization is a soil remediation technique that aims at reducing the metal concentration in the soil solution by adding a binding material to the soil. Application of this technique requires understanding of underlying mechanisms and potential side effects.Both laboratory experiments and model calculations have been performed to gain insight in immobilizing processes. It is essential to quantify metal binding to natural organic matter. The NICA-Donnan model was designed to calculate metal binding by organic materials, but specific Zn parameters were not available due to a lack of analytical data. The Wageningen Donnan Membrane technique (WDMT) was therefore further developed to measure free Zn concentrations in humic acid solutions.Many immobilizing materials increase the soil pH. This results in an increased negative charge of soil particles, and hence in a decreased metal mobility. In some cases, the addition of alkaline materials simultaneously increases the dissolved organic matter (DOM) concentration in the soil solution, resulting in increased leaching of metal-DOM complexes. We showed that alkaline soil amendments need to contain enough Ca to suppress the dispersion of organic matter as induced by a pH increase. We also quantified the dispersion of organic matter. It appeared that the Donnan potential of the organic matter, as calculated by the NICA-Donnan model, correlated very well with the DOM concentration.The addition of alkaline materials strongly decreased the metal concentration in Swiss chard ( Beta vulgaris L. var. cicla ). In contrast, the uptake of Cd and Zn by earthworms ( Lumbricus rubellus and Eisenia veneta ) was hardly influenced by the addition of alkaline materials. Another experiment showed that the addition of MnO 2 , which did not affect soil pH, resulted in a decreased Cd concentration in the earthworm tissue. Apparently, next to dermal uptake, pH independent Cd uptake via the intestine was an important uptake route. Cd uptake by earthworms could be estimated by a soil extraction with 0.1 M triethanolamine and 0.01 M CaCl 2 adjusted to pH 7.2.</font

Тренинговые услуги в украинском туризме: состояние, тенденции, проблемы

Целью данной статьи является анализ места и роли тренингов в системе туризма Украины на фоне общемировых тенденций

Transport-limited kinetics of phosphate retention on iron-coated sand and practical implications

Iron-coated sand (ICS) is a by-product from drinking water treatment made of sand coated with ferric iron (hydr)oxides. It is considered a suitable material for large-scale measures for phosphate removal from natural and agricultural waters to prevent eutrophication. Previous studies demonstrated that the residence time of water must be very long to reach equilibrium partitioning between phosphate and ICS but specifics for application are missing. First, SEM-EDX images were used to support the conceptual assumption that P adsorption inside the coating is a transport-limited process. Second, a conceptual model of phosphate adsorption was proposed considering two types of sites: one type with fast adsorption kinetics and reaching equilibrium with the percolating solution, and another type for which adsorption is also reversible but described by pseudo-first-order kinetics. The latter is conceived to account for transport-limited adsorption in the interior of the coating while the former fraction of sites is assumed to be easily accessible and located close to the grain surface. Third, the kinetics of phosphate adsorption on ICS were quantitatively determined to describe and predict phosphate retention in filters under various flow conditions. The model was calibrated and validated with long-term column experiments, which lasted for 3500 h to approach equilibrium on the slowly reacting sites. The model reproduced the outflowing phosphate concentrations: the pronounced increase after a few pore volumes and the slow increase over the remaining part of the experiment. The parameterized model was also able to predict the time evolution of phosphate concentrations in the outflow of column experiments with different flow velocities, flow interruption, and in desorption experiments. The equilibrium partition coefficient for the experimental conditions was identified as 28.1 L/g-Fe at pH 6.8 and a phosphate concentration of 1.7 mg-P / L. The optimized first-order mass transfer coefficient for the slow adsorption process was 1.56 10 -4 h -1, implying that the slow adsorption process has a time scale of several months. However, based on the parameterized model, the slow adsorption process accounted for 95.5% of the equilibrium adsorption capacity, emphasizing the potential relevance of this process for practical applications. The implications for the design, operation, and lifespan of ICS filters are exemplarily illustrated for different scenarios

Mediterranean Style Diet and Kidney Function Loss in Kidney Transplant Recipients

Background and objectives Despite improvement of short-term graft survival over recent years, long-term graft survival after kidney transplantation has not improved. Studies in the general population suggest the Mediterranean diet benefits kidney function preservation. We investigated whether adherence to the Mediterranean diet is associated with kidney outcomes in kidney transplant recipients. Design, setting, participants, & measurements We included 632 adult kidney transplant recipients with a functioning graft for ≥1 year. Dietary intake was inquired using a 177-item validated food frequency questionnaire. Adherence to the Mediterranean diet was assessed using a nine-point Mediterranean Diet Score. Primary end point of the study was graft failure and secondary end points included kidney function decline (doubling of serum creatinine or graft failure) and graft loss (graft failure or death with a functioning graft). Cox regression analyses were used to prospectively study the associations of the Mediterranean Diet Score with study end points. Results During median follow-up of 5.4 (interquartile range, 4.9–6.0) years, 76 participants developed graft failure, 119 developed kidney function decline, and 181 developed graft loss. The Mediterranean Diet Score was inversely associated with all study end points (graft failure: hazard ratio [HR], 0.68; 95% confidence interval [95% CI], 0.50 to 0.91; kidney function decline: HR, 0.68; 95% CI, 0.55 to 0.85; and graft loss: HR, 0.74; 95% CI, 0.63 to 0.88 per two-point increase in Mediterranean Diet Score) independent of potential confounders. We identified 24-hour urinary protein excretion and time since transplantation to be an effect modifier, with stronger inverse associations between the Mediterranean Diet Score and kidney outcomes observed in participants with higher urinary protein excretion and participants transplanted more recently. Conclusions Adherence to the Mediterranean diet is associated with better kidney function outcomes in kidney transplant recipients.</p

Remnant lipoprotein cholesterol is associated with incident new onset diabetes after transplantation (NODAT) in renal transplant recipients:results of the TransplantLines Biobank and cohort Studies

BACKGROUND: New onset diabetes after transplantation (NODAT) is a frequent and serious complication of renal transplantation resulting in worse graft and patient outcomes. The pathophysiology of NODAT is incompletely understood, and no prospective biomarkers have been established to predict NODAT risk in renal transplant recipients (RTR). The present work aimed to determine whether remnant lipoprotein (RLP) cholesterol could serve as such a biomarker that would also provide a novel target for therapeutic intervention. METHODS: This longitudinal cohort study included 480 RTR free of diabetes at baseline. 53 patients (11%) were diagnosed with NODAT during a median [interquartile range, IQR] follow-up of 5.2 [4.1–5.8] years. RLP cholesterol was calculated by subtracting HDL and LDL cholesterol from total cholesterol values (all directly measured). RESULTS: Baseline remnant cholesterol values were significantly higher in RTR who subsequently developed NODAT (0.9 [0.5–1.2] mmol/L vs. 0.6 [0.4–0.9] mmol/L, p = 0.001). Kaplan-Meier analysis showed that higher RLP cholesterol values were associated with an increased risk of incident NODAT (log rank test, p < 0.001). Cox regression demonstrated a significant longitudinal association between baseline RLP cholesterol levels and NODAT (HR, 2.27 [1.64–3.14] per 1 SD increase, p < 0.001) that remained after adjusting for plasma glucose and HbA1c (p = 0.002), HDL and LDL cholesterol (p = 0.008) and use of immunosuppressive medication (p < 0.001), among others. Adding baseline remnant cholesterol to the Framingham Diabetes Risk Score significantly improved NODAT prediction (change in C-statistic, p = 0.01). CONCLUSIONS: This study demonstrates that baseline RLP cholesterol levels strongly associate with incident NODAT independent of several other recognized risk factors

Food Literacy Is Associated With Adherence to a Mediterranean-Style Diet in Kidney Transplant Recipients

Objectives Adherence to a Mediterranean-style diet is associated with improved health outcomes in kidney transplant recipients (KTR). However, poor dietary habits, including excessive sodium intake, are common in KTR, indicating difficulties with incorporating a healthy diet into daily life. Food literacy is identified as potential facilitator of a healthy diet, but the precise relationship between food literacy and dietary intake in KTR has not been investigated. This study examined food literacy levels in KTR and its association with adherence to a Mediterranean-style diet and sodium intake. Methods This cross-sectional study is part of the TransplantLines Cohort and Biobank Study. Food literacy was measured with the Self-Perceived Food Literacy (SPFL) questionnaire. Dietary intake assessment with food frequency questionnaires was used to calculate the Mediterranean Diet Score. Sodium intake was based on the 24-hour urinary sodium excretion rate. Associations of SPFL with Mediterranean Diet Score and sodium intake were assessed with univariable and multivariable linear regression analyses. Results In total, 148 KTR (age 56 [48-66]; 56% male) completed the SPFL questionnaire with a mean SPFL score of 3.63 ± 0.44. Higher SPFL was associated with a higher Mediterranean Diet Score in KTR (β = 1.51, 95% confidence interval 0.88-2.12, P ≤ .001). Although KTR with higher food literacy tended to have a lower sodium intake than those with lower food literacy (P = .08), the association of food literacy with sodium intake was not significant in a multivariable regression analysis (β = 0.52 per 10 mmol/24-hour increment, 95% confidence interval −1.79 to 2.83, P = .66). Conclusions Higher levels of food literacy are associated with better adherence to a Mediterranean-style diet in KTR. No association between food literacy and sodium intake was found. Further studies are needed to determine if interventions on improving food literacy contribute to a healthier diet and better long-term outcomes in KTR

Association of Circulating Trimethylamine N-Oxide and Its Dietary Determinants with the Risk of Kidney Graft Failure:Results of the TransplantLines Cohort Study

BACKGROUND: Due to the critical shortage of kidneys for transplantation, the identification of modifiable factors related to graft failure is highly desirable. The role of trimethylamine-N-oxide (TMAO) in graft failure remains undetermined. Here, we investigated the clinical utility of TMAO and its dietary determinants for graft failure prediction in renal transplant recipients (RTRs). METHODS: We included 448 RTRs who participated in the TransplantLines Cohort Study. Cox proportional-hazards regression analyses were performed to study the association of plasma TMAO with graft failure. Net Benefit, which is a decision analysis method, was performed to evaluate the clinical utility of TMAO and dietary information in the prediction of graft failure. RESULTS: Among RTRs (age 52.7 ± 13.1 years; 53% males), the baseline median TMAO was 5.6 (3.0-10.2) µmol/L. In multivariable regression analysis, the most important dietary determinants of TMAO were egg intake (Std. β = 0.09 [95%CI, 0.01; 0.18]; p = 0.03), fiber intake (Std. β = -0.14 [95%CI, -0.22, -0.05]; p = 0.002), and fish and seafood intake (Std. β = 0.12 [95%CI, 0.03,0.21]; p = 0.01). After a median follow-up of 5.3 (4.5-6.0) years, graft failure was observed in 58 subjects. TMAO was associated with an increased risk of graft failure, independent of age, sex, the body mass index (BMI), blood pressure, lipids, albuminuria, and the Estimated Glomerular Filtration Rate (eGFR) (Hazard Ratio per 1-SD increase of TMAO, 1.62 (95% confidence interval (CI): 1.22; 2.14, p < 0.001)). A TMAO and dietary enhanced prediction model offered approximately double the Net Benefit compared to a previously reported, validated prediction model for future graft failure, allowing the detection of 21 RTRs per 100 RTRs tested, with no false positives versus 10 RTRs, respectively. CONCLUSIONS: A predictive model for graft failure, enriched with TMAO and its dietary determinants, yielded a higher Net Benefit compared with an already validated model. This study suggests that TMAO and its dietary determinants are associated with an increased risk of graft failure and that it is clinically meaningful

Ultra-processed foods and risk of all-cause mortality in renal transplant recipients

Background Renal transplant recipients (RTRs) have a 6-fold higher risk of mortality than age- and sex-matched controls. Whether high consumption of ultra-processed foods is associated with survival in RTRs is unknown. Objectives We aimed to study the association between high consumption of ultra-processed foods and all-cause mortality in stable RTRs. Methods We conducted a prospective cohort study in adult RTRs with a stable graft. Dietary intake was assessed using a validated 177-item FFQ. Food items were categorized according to the NOVA classification system and the proportion ultra-processed foods comprised of total food weight per day was calculated. Results We included 632 stable RTRs (mean ± SD age: 53.0 ± 12.7 y, 57% men). Mean ± SD consumption of ultra-processed foods was 721 ± 341 g/d (28% of total weight of food intake), whereas the intake of unprocessed and minimally processed foods, processed culinary ingredients, and processed foods accounted for 57%, 1%, and 14%, respectively. During median follow-up of 5.4 y [IQR: 4.9–6.0 y], 129 (20%) RTRs died. In Cox regression analyses, ultra-processed foods were associated with all-cause mortality (HR per doubling of percentage of total weight: 2.13; 95% CI: 1.46, 3.10; P < 0.001), independently of potential confounders. This association was independent from the quality of the overall dietary pattern, expressed by the Mediterranean Diet Score (MDS) or Dietary Approaches to Stop Hypertension (DASH) score. When analyzing ultra-processed foods by groups, only sugar-sweetened beverages (HR: 1.21; 95% CI: 1.05, 1.39; P = 0.007), desserts (HR: 1.24; 95% CI: 1.02, 1.49; P = 0.03), and processed meats (HR: 1.87; 95% CI: 1.22, 2.86; P = 0.004) were associated with all-cause mortality. Conclusions Consumption of ultra-processed foods, in particular sugar-sweetened beverages, desserts, and processed meats, is associated with a higher risk of all-cause mortality after renal transplantation, independently of low adherence to high-quality dietary patterns, such as the Mediterranean diet and the DASH diet. This trial was registered at clinicaltrials.gov as NCT02811835

Processes controlling the flux of legacy phosphorus to surface waters at the farm scale

Phosphorus (P) leaching from agriculture is a major driver of water eutrophication in downstream rivers and lakes. In drained lowland areas with intensive agriculture, a reduction in the fertilizer applications may be insufficient to improve the water quality in the short term as the P accumulated in the soil during decades of high fertilization may continue leaching for many years. A complementary approach to reduce P exports from agriculture is to implement edge-of-field mitigation measures at the farm scale. The selection of effective measures requires a detailed insight into the chemical and hydrological transport mechanisms. Here, we determined the main P sources, processes, and transport routes at the farm scale to support the selection of appropriate mitigation measures. We quantified the legacy P, the different P pools stored in the upper soil, and related it to the yearly P export downstream. To do this, we combined high-resolution monitoring data from the soil, groundwater, surface water, and ditch sediments. The legacy P in the topsoil was high, about 2500 kg ha-1. The predominant subsurface flow and the subsoils' P sorption capacity retained the P mobilized from the topsoil and explained the relative moderate flux of P to surface waters (0.04 kg ha-1 during the 2018-2019 drainage season). The dissolved P entering the drainage ditch via groundwater discharge was bound to iron-containing particles formed due to the oxidation of dissolved ferrous iron. Once leached from the soil to the drainage ditch, resuspension of P-rich sediment particles during flow peaks were the most important P transport mechanism (78%). Therefore, we expect that hydraulic constructions that reduce flow velocities and promote sedimentation of P-containing particles could reduce the export of P further downstream