13 research outputs found
Recurring side-chain triads in monomeric enzymatic structures
An algorithm was developed to exhaustively screen monomeric enzymatic structures for recurring 3-residue side-chain arrangements. The algorithm was used in the screening of two datasets: 100 enzymatic structures with a recurring 3-residue active site, and 100 enzymatic structures with a unique 3-residue active site. In each structure, the algorithm considered all distinct side-chain triads that can be compiled from the entire complement of residues in a single isolated chain. Increasing chain length demonstrated a logarithmic growth in the number of recurring triads. The distribution of total distances in recurring triads adhered to normality while the distribution of unique triad distances appeared negatively skewed. Analysis of variance indicated that the means of maximum and average total distances are significantly greater in unique triads than in recurring triads. Screening for recurring triads in synthetically generated alternative rotamer structures demonstrated an overall decrease in the percent of recurring triads, as compared to the natural structures
Comprehensive and unbiased multiparameter high-throughput screening by compaRe finds effective and subtle drug responses in AML models
Large-scale multiparameter screening has become increasingly feasible and straightforward to perform thanks to developments in technologies such as high-content microscopy and high-throughput flow cytometry. The automated toolkits for analyzing similarities and differences between large numbers of tested conditions have not kept pace with these technological developments. Thus, effective analysis of multiparameter screening datasets becomes a bottleneck and a limiting factor in unbiased interpretation of results. Here we introduce compaRe, a toolkit for large-scale multiparameter data analysis, which integrates quality control, data bias correction, and data visualization methods with a mass-aware gridding algorithm-based similarity analysis providing a much faster and more robust analyses than existing methods. Using mass and flow cytometry data from acute myeloid leukemia and myelodysplastic syndrome patients, we show that compaRe can reveal interpatient heterogeneity and recognizable phenotypic profiles. By applying compaRe to high-throughput flow cytometry drug response data in AML models, we robustly identified multiple types of both deep and subtle phenotypic response patterns, highlighting how this analysis could be used for therapeutic discoveries. In conclusion, compaRe is a toolkit that uniquely allows for automated, rapid, and precise comparisons of large-scale multiparameter datasets, including high-throughput screens.Peer reviewe
Dysregulated Glial Differentiation in Schizophrenia May Be Relieved by Suppression of SMAD4- and REST-Dependent Signaling
Cell-Intrinsic Astrocytic Pathology is Conserved Across Both Species and Models of Huntington Disease
Cell-intrinsic glial pathology is conserved across human and murine models of Huntington's disease
Estimation of protein function using template-based alignment of enzyme active sites
BACKGROUND: The accumulation of protein structural data occurs more rapidly than it can be characterized by traditional laboratory means. This has motivated widespread efforts to predict enzyme function computationally. The most useful/accurate strategies employed to date are based on the detection of motifs in novel structures that correspond to a specific function. Functional residues are critical components of predictively useful motifs. We have implemented a novel method, to complement current approaches, which detects motifs solely on the basis of distance restraints between catalytic residues. RESULTS: ProMOL is a plugin for the PyMOL molecular graphics environment that can be used to create active site motifs for enzymes. A library of 181 active site motifs has been created with ProMOL, based on definitions published in the Catalytic Site Atlas (CSA). Searches with ProMOL produce better than 50% useful Enzyme Commission (EC) class suggestions for level 1 searches in EC classes 1, 4 and 5, and produce some useful results for other classes. 261 additional motifs automatically translated from Jonathan Barker’s JESS motif set [Bioinformatics 19:1644–1649, 2003] and a set of NMR motifs is under development. Alignments are evaluated by visual superposition, Levenshtein distance and root-mean-square deviation (RMSD) and are reasonably consistent with related search methods. CONCLUSION: The ProMOL plugin for PyMOL provides ready access to template-based local alignments. Recent improvements to ProMOL, including the expanded motif library, RMSD calculations and output selection formatting, have greatly increased the program’s usability and speed, and have improved the way that the results are presented
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