Synthesis of Resveratrol Ester Derivatives Using Selective Enzymatic Hydrolysis.

Resveratrol, a naturally derived stilbene, is an interesting compound mostly talked about recently because for its anti-cancer properties. Unfortunately it has some shortcomings due to its low bioavailability and low solubility in water. For this reason, my research is to overcome resveratrol\u27s drawbacks by improving its bioavailability and hydrophilicity. My research is focused on syntheses of novel derivatives of resveratrol such as 3, 5-di-O-isobutyroyl resveratrol and 3, 5-di-O-hexanoyl resveratrol using lipase catalyzed hydrolysis. Therefore, the tri-acylated resveratrols 3, 5, 4\u27-tri-O-isobutyroyl resveratrol and 3, 5,4\u27-tri-O-hexanoyl resveratrol were first synthesized. 3,5,4\u27-tri-O-isobutyroyl resveratrol and 3,5,4\u27-tri-O-hexanoyl resveratrol were then hydrolyzed using lipase (C. Antarctica) to obtain the products 3,5-di-O-isobutyroyl resveratrol and 3,5-di-O-hexanoyl resveratrol. The four compounds 3,5-di-O-isobutyroyl resveratrol, 3,5-di-O-hexanoyl resveratrol, 3,5,4\u27-tri-O-hexanoyl resveratrol, and 3,5-di-O-hexanoyl resveratrol were characterized by 1H NMR and 13C NMR

Preliminary Studies on the Use of Sawdust and Peanut Shell Powder as Adsorbents for Phosphorus Removal from Water

Phosphorus is one of the key nutrients that contribute to eutrophication and excess algal growth in rivers and lakes and other surface water bodies. Such algal blooms affect the life of aquatic organisms, especially fishes, and block sunlight from reaching bottom dwelling plants thus inhibiting photosynthesis and stunting growth. In this study, sawdust and peanut shell powder were explored as adsorbents for the removal of phosphorus from aqueous solutions. A series of batch experiments were conducted to determine the effects of adsorbent dosage, initial phosphorus concentration and contact time on the rate of phosphorus removal. The results showed that 78% and 39% of phosphorus was removed by sawdust and peanut shell powder respectively at adsorbate concentrations of 10 mg/l (total P) for a period of 180 min at an adsorbent dosage of 0.4 g. It was also found that the highest phosphorus removal occurred at adsorbent mass of 0.4 g of sawdust and 0.6 for peanut shell powder. Adsorption data were fitted to the linearised forms of the Freundlich and Langmuir isotherm models to determine the water - adsorbent partitioning coefficient. Sawdust proved to be a better adsorbent than peanut shell powder

Incidence, prevalence and severity of fall armyworm infestation in Ghana: a case of two maize enclaves in the Ashanti Region of Ghana

Maize production is an important enterprise in Ghana, providing livelihood for thousands of people. It is challenged by fall armyworm infestation causing destruction to many cultivated lands in production enclaves and threatening food security. Effective control, however, requires information on the actual pest infestation as well as the effect of climate factors on infestation in these enclaves. The study assessed the incidence, prevalence and severity of fall armyworm infestation on maize farms in two major maize enclaves (Ejura and Ejisu) in Ghana. Data was taken on the presence, infestation levels and damage of fall armyworms as well as the climatic conditions in each district. Data collection was done by sampling 50 maize plants each on 40 maize farms in both districts and assessing them for the incidence, larval prevalence, leaf damage and severity. Results showed varying infestation in both districts (p < 0.0001) with Ejisu having a higher prevalence (0.10 ± 0.04 larvae per plant) than Ejura (0.05 ± 0.03 larvae per plant) in the minor season at seedling stage. At the vegetative stage, Ejisu recorded a higher prevalence in both seasons. A low severity was recorded at the seedling stage in both districts for all seasons which, however, varied among seasons at vegetative stage. Climatic variables including rainfall, temperature, relative humidity and wind were found to significantly impact infestation in both districts. The study thus, showed fall armyworm infestation to be a major challenge to maize production in both districts confirming it as a major constraint to maize production in the region

West Africa's moist convective environment as observed by the Atmospheric Infrared Sounder (AIRS)

Knowledge of the seasonal positioning of the Intertropical Discontinuity (ITD) is critical to understanding seasonal moist convective processes and associated rainfall over West Africa. This study constitutes a new analysis of the seasonality of moist convection over West Africa, relative to the ITD, based on NASA's Atmospheric Infrared Sounder (AIRS) measurements from 2003 to 2018. Results show that AIRS resolves the seasonal march of the ITD, including its inherent diurnal-scale variations. AIRS captures the north–south daytime skin temperature dipole around the ITD, with greater relative temperatures to the north, especially during March–August. In the vicinity of the nighttime ITD, AIRS profiles indicate increased instability that is characteristic of nocturnal thunderstorm propagation. For seven Ghana weather stations, we show that AIRS positive moisture and equivalent potential temperature anomalies coincide with observed thunderstorm days. On these thunderstorm days, the mean latitude of the AIRS-derived ITD is displaced 3°, 0.2°, and 2° north of its DJF, MAM, and SON climatological positions, respectively, and 1.2° south in JJA. Among four common thunderstorm initiation indices considered, the K-index is determined to be most skillful. The findings of this study contribute to the Global Challenges Research Fund, African Science for Weather Information and Forecasting Techniques project's mission to build local tropical weather forecasting capacity and capabilities in West Africa

Revisiting the agro-climatic zones of Ghana: a re-classification in conformity with climate change and variability

The Ghana Meteorological Agency delineated Ghana’s geographical space into four agro-climatic zones namely the north, transition, forest and coastal zones. Since the demarcation in the 1960s, previous studies have rarely provided a more dis-aggregated agro-climatic zone map in tandem with contemporary climate change and variability. The continued use of this age-old classified zones is a disservice to the public. In this study, therefore, we evaluated the existing agro-climatic zone map of Ghana and reconstructed it to a more appropriate and dis-aggregated map that reflect current climate change and variability impact. This was achieved by quantifying the contrast in rainfall and temperature amount over a 30 year period for different climate windows and mapped out areas with similar rainfall and temperature regimes. Our findings revealed significant changes in the existing agro-climatic zones especially in terms of number, the boundary size and geographical orientation of the zones. The newly proposed map consist of five distinctive climate zones namely: the Sudan Savannah, Guinea Savannah, Transition, Forest and Coastal zones. The Sudan and Guinea Savannah zones showed a southerly expansion. The transition zone shriveled in size as the Guinea Savannah zone took over most of it, notably in the southeast. The forest zone shrank in size with a northwest shift while the coastal belt grew to encompass the whole coast of Ghana. These changes are strong evidence of climate change and possible food production changes. These findings are useful to agriculture sector in planning their activities, the health sector in predicting specific diseases caused by changes in weather and climate, Ghana Meteorological Agency for weather forecasting purposes, and the National Disaster Management in identifying disaster prone zones

Empowering citizen-led adaptation to systemic climate change risks

The increasing impacts of climate change instigate the need for adaptation. However, most adaptation initiatives focus on actions by government or businesses, despite growing calls for communities on the frontline of climate risks to be involved in planning and selecting strategies. Here, we appraise a pilot process using participatory systems mapping with citizens to identify 1) diverse threat vectors for local climate impacts and 2) context-relevant interventions to protect households and communities while 3) considering synergies and tradeoffs with other socially desirable outcomes. We tested the pilot process in communities in the lower Volta Basin in Ghana, the Assam region in India, and Southern England. From participants' perspectives, the process increased awareness of- and preparedness for climate change impacts and raised essential learning points for upscaling citizen-led adaptation approaches. These include understanding multiple outcomes of interventions, barriers, and enablers to implementation, and sensitivity of co-design to regional geography and socio-cultural context

Basic science232. Certolizumab pegol prevents pro-inflammatory alterations in endothelial cell function

Background: Cardiovascular disease is a major comorbidity of rheumatoid arthritis (RA) and a leading cause of death. Chronic systemic inflammation involving tumour necrosis factor alpha (TNF) could contribute to endothelial activation and atherogenesis. A number of anti-TNF therapies are in current use for the treatment of RA, including certolizumab pegol (CZP), (Cimzia ®; UCB, Belgium). Anti-TNF therapy has been associated with reduced clinical cardiovascular disease risk and ameliorated vascular function in RA patients. However, the specific effects of TNF inhibitors on endothelial cell function are largely unknown. Our aim was to investigate the mechanisms underpinning CZP effects on TNF-activated human endothelial cells. Methods: Human aortic endothelial cells (HAoECs) were cultured in vitro and exposed to a) TNF alone, b) TNF plus CZP, or c) neither agent. Microarray analysis was used to examine the transcriptional profile of cells treated for 6 hrs and quantitative polymerase chain reaction (qPCR) analysed gene expression at 1, 3, 6 and 24 hrs. NF-κB localization and IκB degradation were investigated using immunocytochemistry, high content analysis and western blotting. Flow cytometry was conducted to detect microparticle release from HAoECs. Results: Transcriptional profiling revealed that while TNF alone had strong effects on endothelial gene expression, TNF and CZP in combination produced a global gene expression pattern similar to untreated control. The two most highly up-regulated genes in response to TNF treatment were adhesion molecules E-selectin and VCAM-1 (q 0.2 compared to control; p > 0.05 compared to TNF alone). The NF-κB pathway was confirmed as a downstream target of TNF-induced HAoEC activation, via nuclear translocation of NF-κB and degradation of IκB, effects which were abolished by treatment with CZP. In addition, flow cytometry detected an increased production of endothelial microparticles in TNF-activated HAoECs, which was prevented by treatment with CZP. Conclusions: We have found at a cellular level that a clinically available TNF inhibitor, CZP reduces the expression of adhesion molecule expression, and prevents TNF-induced activation of the NF-κB pathway. Furthermore, CZP prevents the production of microparticles by activated endothelial cells. This could be central to the prevention of inflammatory environments underlying these conditions and measurement of microparticles has potential as a novel prognostic marker for future cardiovascular events in this patient group. Disclosure statement: Y.A. received a research grant from UCB. I.B. received a research grant from UCB. S.H. received a research grant from UCB. All other authors have declared no conflicts of interes

Dimethyl fumarate in patients admitted to hospital with COVID-19 (RECOVERY): a randomised, controlled, open-label, platform trial

Dimethyl fumarate (DMF) inhibits inflammasome-mediated inflammation and has been proposed as a treatment for patients hospitalised with COVID-19. This randomised, controlled, open-label platform trial (Randomised Evaluation of COVID-19 Therapy [RECOVERY]), is assessing multiple treatments in patients hospitalised for COVID-19 (NCT04381936, ISRCTN50189673). In this assessment of DMF performed at 27 UK hospitals, adults were randomly allocated (1:1) to either usual standard of care alone or usual standard of care plus DMF. The primary outcome was clinical status on day 5 measured on a seven-point ordinal scale. Secondary outcomes were time to sustained improvement in clinical status, time to discharge, day 5 peripheral blood oxygenation, day 5 C-reactive protein, and improvement in day 10 clinical status. Between 2 March 2021 and 18 November 2021, 713 patients were enroled in the DMF evaluation, of whom 356 were randomly allocated to receive usual care plus DMF, and 357 to usual care alone. 95% of patients received corticosteroids as part of routine care. There was no evidence of a beneficial effect of DMF on clinical status at day 5 (common odds ratio of unfavourable outcome 1.12; 95% CI 0.86-1.47; p = 0.40). There was no significant effect of DMF on any secondary outcome

Inter-Comparison of AIRS Temperature and Relative Humidity Profiles with AMMA and DACCIWA Radiosonde Observations over West Africa

The vertical profiles of temperature and water vapour from the Atmospheric InfraRed Sounder (AIRS) have been validated across various regions of the globe as an effort to provide a substitute for radiosonde observations. However, there is a paucity of inter-comparisons over West Africa where local convective processes dominate and radiosonde observations (RAOBs) are limited. This study validates AIRS temperature and relative humidity profiles for selected radiosonde stations in West Africa. Radiosonde data were obtained from the AMMA and DACCIWA campaigns which spanned 2006&ndash;2008 and June&ndash;July 2016 respectively and offered a period of prolonged radiosonde observations in West Africa. AIRS performance was evaluated with the bias and root mean square difference (RMSD) at seven RAOB stations which were grouped into coastal and inland. Evaluation was performed on diurnal and seasonal timescales, cloud screening conditions and derived thunderstorm instability indices. At all timescales, the temperature RMSD was higher than the AIRS accuracy mission goal of &plusmn;1 K. Relative humidity RMSD was satisfactory with deviations &lt;20% and &lt;50% for both lower and upper troposphere respectively. AIRS retrieval of water vapour under cloudy and cloud-free conditions had no significant difference whereas cloud-free temperature was found to be more accurate. The seasonal evolution of some thunderstorm convective indices were also found to be comparable for AIRS and RAOB. The ability of AIRS to capture the evolution of these indices imply it will be a useful dataset for the African Science for Weather Information and Forecasting Techniques (SWIFT) high impact weather studies

Economics analysis of Seed Yam production in Aeroponic system

The supply of seed yams for intensive yam production is hindered by many constraints, including diseases and pest infestations as well as the unavailability of quality planting material. The combination of tissue culture and aeroponics system is perceived to be the way towards clean and adequate supply of seed yam for enhanced yam production. However, the system is considered as expensive for any individual to implement. In order to encourage private sector to participate in this venture, the economic analysis determining the feasibility and viability of using aeroponics in seed yam production was performed. Using data from established tissue culture and aeroponics system in Ghana, the fixed cost and variable cost parameters as well as production costs were obtained. Results revealed that total cost of building aeroponics structure amounted to GH₵ 94,178.00 (USD 17,938.70). Annual cost of aeroponics structure was GH₵ 9,417.82 (USD 1,793.87). Annual total cost of production totalled GH₵ 204,391.75 (USD 38,931.61). Annual net revenue was GH₵ 75,888.00 (USD 14,454.86). Payback period was 15 months and benefit cost ratio was 1.4. Aeroponics system for seed yam production is therefore profitable since short period would be needed to recoup investment. For food security and creation of workplaces, government could partner with the private sector in the establishment of aeroponics systems to increase yam production and export