The incidence of cancer and potential role of sirolimus immunosuppression conversion on mortality among a single-center renal transplantation cohort of 1,816 patients

INTRODUCTION: The chronic use of immunosuppressive drugs in renal transplant recipients increases the risk of developing de novo malignancies. Herein we analyze the incidence of de novo tumors and the potential role of sirolimus to improve cancer-specific survival among a cohort at a single center. METHODS: This retrospective analysis of our 1,816 patients allografted between January 1983 and December 2009 sought subjects who developed de novo tumors. Epidemiological and clinical data were examined using Mann-Whitney and Pearson's chi-square or Fisher exact tests for statistical comparisons of continuous and categorical variables, respectively. Kaplan-Meier survival curves were used to determine cancer-specific survival according to type of neoplasia and immunosuppressive regimen, namely, conversion to sirolimus. RESULTS: One hundred patients (5.5%) were diagnosed with a de novo malignancy. The 110 different cancers were diagnosed at a median interval of 73 months after kidney transplantation. The overall cancer-specific survivals at 1 and 5 years after cancer diagnosis were 87.0% and 76.9%, respectively. The 15 patients converted to sirolimus showed no difference in survival. CONCLUSION: The observed frequencies of cancer in our center are consistent with the literature. Among our cohort, sirolimus did not significantly impact survival among subjects who had de novo malignancies

Competitividade de um grupo de produtores da cadeia produtiva da maçã na região de Vacaria/RS.

O objetivo deste trabalho foi identificar se um grupo de produtores da cadeia produtiva da maçã na microrregião dos Campos de Cima da Serra está enfrentando dificuldades, e quais seriam elas, bem como identificar possíveis oportunidades que possam impactar na continuidade desta cadeia, através da metodologia de pesquisa exploratória, com caráter qualitativo e quantitativo quanto à abordagem metodológica, e, descritivo-explicativa quanto aos objetivos e levantamento de bibliografia. A coleta de informações foi realizada pelo método de entrevistas semiestruturadas, por meio de questionário. Os resultados indicaram que os produtores vêm enfrentando problemas de ordem produtiva e comercial, como fatores vinculados à mão de obra e clima, estabilização de preços, baixo consumo nacional da fruta, dentre outros, podendo ser amenizados pela introdução de técnicas, variedades e controle de custos. Além disso, a introdução paralela de novas culturas similares, como uva e pêra, pode aperfeiçoar o processo produtivo pela redução do custo. Palavras-chave: Maleicultura, produtividade, competitividade

NEFRECTOMIA PARCIAL – A EXPERIÊNCIA DE 10 ANOS DO HOSPITAL DE SANTO ANTÓNIO

A nefrectomia parcial (NP) é uma técnica reconhecida no tratamento de diferentes patologias renais,nomedamente na oncológica. Os autores apresentam a sua casuística dos últimos 10 anos, com avaliação dos índices de recorrência e sobrevivência

Transvesical peritoneoscopy with rigid scope: feasibility study in human male cadaver

BACKGROUND: Transvesical port refers to the method of accessing the abdominal cavity through a natural orifice (i.e., urethra) under endoscopic visualization. Since its introduction in 2006, various reports have been published describing different surgical interventions using a rigid ureteroscope in a porcine model. The aim of this study was to test the access and feasibility of peritoneoscopy by using a rigid ureteroscope in a human male cadaver. METHODS: Two adult male cadavers were used to perform the procedures. A rigid ureteroscope was used for the creation of transvesical access into the peritoneal cavity. Peritoneoscopy, liver biopsy, and identification and manipulation of the ileocecal appendix were performed. RESULTS: Transvesical access into the peritoneal cavity was quickly established. The rigid ureteroscope easily allowed visualization of the abdominal cavity with good image quality. Liver biopsy and manipulation of ileocecal appendix were carried out without difficulties. CONCLUSIONS: Peritoneoscopy, liver biopsy, and ileocecal appendix manipulation using a rigid ureteroscope through a transvesical port is feasible in a cadaver model. The development of a specific rigid scope for the transvesical port might herald a promising future for this NOTES access

Caracterização da composição tecidual da perna de cabritos do Alto Camaquã sacrificados aos 12 meses de idade.

CIC ENPOS

Automatic solid-phase extraction by programmable flow injection coupled to chromatographic fluorimetric determination of fluoroquinolones

Fluoroquinolones are broad-spectrum bactericidal agents applied for the treatment of human and veterinary diseases. Their common use and their incorrect disposal foster environmental contamination, namely in water resources, increasing the risk of antimicrobial resistance. Hence, a method based on automatic solid-phase extraction coupled to liquid chromatography and fluorimetric detection is proposed for the determination of fluoroquinolones in environmental waters. For the solid-phase extraction procedure, a commercially available molecularly imprinted polymer targeting fluoroquinolones was trapped inside a flow-through extraction column, integrated into a programmable flow injection system using multisyringe flow injection analysis, where all steps concerning sorbent conditioning, sample loading, matrix removal, and analyte elution were performed under computer control. The eluate resulting from the sample preparation was collected and transferred at-line to chromatographic analysis using a reversed-phase monolithic column coupled to a fluorimetric detector, and isocratic elution with methanol-phosphoric acid (pH 3.0; 5.0 mM) (17.5:82.5, v/v) at a flow rate of 3.5 mL min-1. Sample treatment and chromatographic analysis were performed in tandem, with sample throughput limited by the sample treatment step. Calibration curves based on fluorescence intensity vs. analyte mass were obtained in the range of 10 to 1000 pg for norfloxacin, ciprofloxacin, and enrofloxacin with LOD values of 6-19 ng L-1 for a sample volume of 100 mL, and RSD < 11% at 0.7 ¿g L-1. The method was successfully applied to estuarine river water analysis.info:eu-repo/semantics/publishedVersio