Progetto di recupero e riqualificazione del Centro Diocesano Giovanile di Carrara

Il complesso edilizio del Centro Diocesano Giovanile di Carrara (MS) fin dagli anni '60 rappresenta un importante punto di riferimento, soprattutto a livello giovanile, nel cuore della città. In questa tesi si affronta il problema della riqualificazione architettonica del complesso,oggi diffusamente degradato e in parte non più utilizzato. Il progetto, teso ad una forte caratterizzazione dell'area, prevede il recupero delle strutture destinate alle attività aggregative/formative e la realizzazione della nuova biblioteca civica multimediale di Carrara

LA TUTELA CAUTELARE NEL PROCESSO TRIBUTARIO

Il presente lavoro si proposto di analizzare l’istituto della tutela cautelare all’interno del processo tributario. Tema che appare di particolare rilevanza in ambito tributario, considerando che la maggior parte degli atti emessi da parte dell’Amministrazione finanziaria, in virtù del potere di c.d. autotutela positiva che l’ordinamento le conferisce, sono immediatamente esecutivi, ovvero possono incidere direttamente nella sfera giuridica del contribuente, senza che sia necessario l’intervento di un giudice. La questione di particolare interesse, su cui maggiormente l’elaborato si concentrerà, verte principalmente sulla recente revisione subita dall’istituto, operata per mezzo del d.lgs. 24 settembre 2015, n. 156, a cui si è voluto offrire un contributo, andando a comprendere come le nuove previsioni operano per il processo tributario ed analizzando le questioni rimaste tuttora irrisolte. Saranno analizzate le questioni relative alla mancanza di forme di tutela cautelare ante causam e la non impugnabilità dell’ordinanza di sospensione, entrambe escluse per il solo processo tributario ed ammesse invece, a seguito dell’evoluzione legislativa e della giurisprudenza di legittimità, tanto nel processo civile che in quello amministrativo. Si proverà quindi a ravvisare e sostenere eventuali profili di incostituzionalità a seguito di tali mancanze. Sarà inoltre analizzata la discussa sospensione anche dei c.d. “atti negativi”, attraverso le sue possibili modalità applicative. Nelle conclusioni finali si farà una valutazione di quello che è l’istituto oggi, con riflessioni critiche sulle effettive esigenze di tutela che necessità il processo tributario. Saranno offerti inoltre degli spunti di evoluzione dell’istituto in relazione all’applicazione delle forme di tutela previste nel rito civile e amministrativo

The Impact of IXPs on the AS-level Topology Structure of the Internet

The AS-level topology of the Internet has been quite a hot research topic in the last few years. However, only a small number of studies have been developed that give a structural interpretation of this graph. Such an interpretation is crucially important in order to test protocols and optimal routing algorithms, to design efficient networks, and for failure detection purposes. Moreover, most research does not highlight the role that IXPs have on the AS-level structure of the Internet, although their role is recognized as fundamental. The initial contribution of this study is an analysis of the most important AS-level topologies that are publicly found on the web and an analysis of the topology obtained when they are merged. We compiled structural information from this topology making considerable use of the k-core decomposition technique to delineate various particular classes of nodes. Next, we associated node properties with a reasonable modus operandi of the ASs on the Internet. The second contribution is a study of the impact that ASs connected to IXPs and BGP connections crossing IXPs have on the AS-level topology. To achieve this, we developed a procedure to gather reliable information related to IXPs and their participants

Pitt-Hopkins syndrome: report of a case with a TCF4 gene mutation

<p>Abstract</p> <p>Aims</p> <p>We will discuss the clinical and genetic diagnosis of a child with severe psychomotor delay, who at 3 years of age presented with paroxysms of hyperpnea-apnea and seizures unrelated to breathing anomalies.</p> <p>Methods</p> <p>The child underwent genetic (karyotype, FISH telomeres) and neuroradiological (cranial CT and MRI) tests, which proved to be normal. He came under our clinical observation at 3 years and 5 months of age. Due to severe psychomotor delay and facial dysmorphisms we completed the genetic investigations based on his clinical feature and analysis of the available literature.</p> <p>Results</p> <p>The presence of severe mental retardation associated with anomalous breathing pattern may suggest the Joubert and Rett syndrome, however these were excluded on the basis of clinical and genetic examination. Angelman syndrome, suspected for facial dysmorphisms and absent language, was also excluded because of the presence of a normal pattern of methylation at SNRPN locus. Another possible diagnosis was the Pitt-Hopkins Syndrome (PHS), characterized by severe mental retardation, breathing anomalies (paroxisms of hyperpnea-apnea), dysmorphisms and sometimes epilepsy. Haploinsufficiency of TCF4 gene located at 18q21.2 region has been recently identified as causative of this syndrome. In our patient the research of TCF4 mutation by the Institute of Human Genetics, University Hospital Erlangen (Germany), showed a de novo mutation.</p> <p>Conclusions</p> <p>The diagnosis of Pitt-Hopkins syndrome, an underdiagnosed cause of mental retardation, was based on clinical and genetic findings. Searching for TCF4 mutations is highly recommended when others overlapping syndromes was excluded. At our knowledge our patient is the first italian case of PHS diagnosed at molecular level.</p

The brain–heart interaction in epilepsy: implications fordiagnosis, therapy, and SUDEP prevention

Epilèpsia; Malalties cardiaques; Mort sobtadaEpilepsia; Enfermedades cardiacas; Muerte súbitaEpilepsy; Heart disease; Sudden deathThe influence of the central nervous system and autonomic system on cardiac activity is being intensively studied, as it contributes to the high rate of cardiologic comorbidities observed in people with epilepsy. Indeed, neuroanatomic connections between the brain and the heart provide links that allow cardiac arrhythmias to occur in response to brain activation, have been shown to produce arrhythmia both experimentally and clinically. Moreover, seizures may induce a variety of transient cardiac effects, which include changes in heart rate, heart rate variability, arrhythmias, asystole, and other ECG abnormalities, and can trigger the development of Takotsubo syndrome. People with epilepsy are at a higher risk of death than the general population, and sudden unexpected death in epilepsy (SUDEP) is the most important direct epilepsy-related cause of death. Although the cause of SUDEP is still unknown, cardiac abnormalities during and between seizures could play a significant role in its pathogenesis, as highlighted by studies on animal models of SUDEP and registration of SUDEP events. Recently, genetic mutations in genes co-expressed in the heart and brain, which may result in epilepsy and cardiac comorbidity/increased risk for SUDEP, have been described. Recognition and a better understanding of brain-heart interactions, together with new advances in sequencing techniques, may provide new insights into future novel therapies and help in the prevention of cardiac dysfunction and sudden death in epileptic individuals

Neutral Solar Wind Detector (NSWD) for Solar Orbiter

The Neutral Solar Wind Detector (NSWD), to be flown on board Solar Orbiter, consists of a neutral atom sensor able to detect and characterize (in terms of velocity and direction) the energetic neutrals flowing together the ionised particles within the solar wind, between ∼0.05 keV/nuc and ∼5 keV/nuc. This may be a stand-alone instrument (indicated as high priority augmentation payload in the Solar Orbiter PDD), but it is also suitable for inclusion in the solar wind particle package SWA. The NSWD primary scientific objectives may be summarized as in the following: • observation of neutral solar wind flux; • velocity, density and temperature of the neutral solar wind; • comprehension of solar Ly-α corona, i.e. deduction of solar wind plasma velocity distributions anisotropy perpendicular and along the solar magnetic field lines from neutral solar wind observations; • study of the solar wind acceleration region via the detection of the neutral solar wind hydrogen atoms and investigation of the temporal and spatial details of the solar wind using the co-aligned movement of the Solar Orbiter spacecraft with respect to the solar rotation; • observation of the fast and slow neutral solar wind in different solar conditions, potentially including transitions regions and CMEs; • resolution of the "inner source" pick-up ion puzzle thought to originate from solar wind plasma - dust interaction in the solar atmosphere region within 0.2 AU

Regenerative medicine in hearing recovery

Hearing loss, or deafness, affects 360 million people worldwide of which about 32 million are children. Deafness is irreversible when it involves sensory hair cell death because the regenerative ability of these cells is lost in mammals after embryo development. The therapeutic strategies for deafness include hearing aids and/or implantable devices. However, not all patients are eligible or truly benefit from these medical devices. Regenerative medicine based on stem cell application could play a role in both improvement of extant medical devices and in vivo recovery of auditory function by regeneration of inner ear cells and neurons. A review of recent literature on the subject indicates that two promising approaches to renewal and differentiation of cochlear tissues are transplantation of stem cells and in situ administration of growth factors. Rather than directly regenerating dead cells, these procedures apparently induce, through various pathways, differentiation of resident cochlear cells. More studies on the possible adverse effects of transplanted cells and the recovery of tonotopic sensorineural activity or required. To date, no reliable clinical results have been obtained in the field of cochlear regeneration. \ua9 2017 International Society for Cellular Therapy

LED lighting for indoor cultivation of basil

Indoor cultivation systems are gaining importance worldwide, thanks to their greater efficiency in the use of resources (water, land and nutrients). The limiting factor for these systems is the illumination costs that are still high. In this context, LEDs (light emitting diodes) are gaining attention because of their ability to provide the required light spectra, and high electricity use efficiency. The goal of this study is to identify the role played by red:blue (R:B) ratio on the resource use efficiency of indoor basil cultivation, linking the light physiological response to changes in yield and nutritional properties. Basil plants were cultivated in growth chamber under 5 different R:B ratio LED lighting regimens (respectively, RB0.5, RB1, RB2, RB3, and RB4), using fluorescent lamps as control (CK1). For the six light treatments, a PPFD of 215 mol m-2 s-1 and a photoperiod of 16/8 light/dark per day were provided. Greater biomass production was associated with LEDs lighting as compared with fluorescent lamp, with best performances observed using RB≥2. Adoption of RB2 and RB3 improved also the plant’s capacity to transform resources, resulting in greatest water, land and energy use efficiency. Nutrient use efficiency was increased by using LED lights with a greater portion of blue light in the spectrum. Decreasing R:B ratio also increased leaf stomatal conductance. Plant grown under RB3 showed the best antioxidant properties in terms of flavonoid content and FRAP as compared to the other light treatments. From this study it can be concluded that a R:B ratio of 3 (RB3) provides optimal growing conditions for indoor cultivation of basil

Pediatric hypnic headache. a systematic review

Introduction: Hypnic headache (HH) is a primary headache, and it is considered a rare condition in children. The underlying mechanisms of HH are not yet fully understood. This systematic review aims to provide a comprehensive description of the clinical features of all published cases of pediatric HH. It will also discuss the differences in headache features between children and adults, the increased diagnostic sensitivity of the new diagnostic criteria (ICHD-3), potential pathophysiological hypotheses explaining the higher incidence in adults, differential diagnoses, and therapeutic options for children. Methods: A systematic search was conducted to identify and analyze articles reporting cases of HH in patients under the age of 18. The search was performed in major medical databases including Cochrane Library, EBSCO, Embase, Medline, PubMed, Science Direct, Scopus, and Web of Science. The search covered the period from 1988 to April 2023. Relevant studies were screened for eligibility, and data extraction was performed using a standardized approach. Results: Seven children with HH were included in the analysis. The mean age of onset for headache attacks was 10 ± 4.3 years (range 3-15 years). The average time from the start of headaches to diagnosis was 15.8 ± 25.0 months (range 1-60 months). Headache features in children differed from those observed in adult HH patients. Children experienced throbbing/pulsating pain, while adults reported dull/pressure-like pain. Children also had lower frequency and shorter duration of attacks compared to adults. The use of ICHD-3 criteria appeared to be more sensitive and inclusive for diagnosing HH in children compared to the previous ICHD-2 criteria. The association of headache attacks with sleep suggests that HH may be a primary disorder with a chronobiological origin. Hypothalamic dysfunction and melatonin dysregulation, which are more prevalent in older individuals, could potentially explain the higher incidence of HH in adults. Other primary headaches and secondary causes should be ruled out. Melatonin prophylactic therapy may be considered for pediatric patients. Discussion: Further evaluation of the clinical features of HH in children is needed. The development of specific diagnostic criteria for pediatric cases could improve diagnostic rates and enhance the management of children with HH

A novel DAG-dependent mechanism links PKCα and cyclin B1 regulating the G2/M progression of cell cycle

Protein kinase C α has been reported to regulate cell cycle in several cell lines. Most of the reports describe a role for PKC α in G1/S transition but little is known about its possible involvement in G2/M progression. Our studies on the effects of PKC inhibitors, PKC α silencing and overexpression demonstrated a novel and positive role for PKC α in cyclin B1 regulation in human erythroleukemia cell line, K562. On the other hand, using PKC inhibitors and a PKC α inactive mutant, we could report that PKC α activity was not necessary for cyclin B1 regulation. Moreover, immunoprecipitation and immunocytochemistry experiments showed that these two proteins could physically interact each other and enter into the nuclei during G2/M progression. In order to better understand this mechanism, we investigated how PKC α could be attracted into the nuclei. We found a high increase of nuclear DAG during the G2/M phase. Then, using PMA and PLC inhibitors, we showed that PKC α translocation was due to the increase in nuclear DAG. Surprisingly, we saw the same effect on cyclin B1. Finally, in order to discover which PLC was involved, we silenced the nuclear localized PLCβ1 founding a decrease in PKC α and cyclin B1 nuclear amount. Taken together, our data demonstrate the existence of a novel DAG dependent mechanism linking PKC α and cyclin B1 which can regulate their entry into the nuclei during the G2/M phase of cell cycle