2,567 research outputs found
PCA-RECT: An Energy-efficient Object Detection Approach for Event Cameras
We present the first purely event-based, energy-efficient approach for object
detection and categorization using an event camera. Compared to traditional
frame-based cameras, choosing event cameras results in high temporal resolution
(order of microseconds), low power consumption (few hundred mW) and wide
dynamic range (120 dB) as attractive properties. However, event-based object
recognition systems are far behind their frame-based counterparts in terms of
accuracy. To this end, this paper presents an event-based feature extraction
method devised by accumulating local activity across the image frame and then
applying principal component analysis (PCA) to the normalized neighborhood
region. Subsequently, we propose a backtracking-free k-d tree mechanism for
efficient feature matching by taking advantage of the low-dimensionality of the
feature representation. Additionally, the proposed k-d tree mechanism allows
for feature selection to obtain a lower-dimensional dictionary representation
when hardware resources are limited to implement dimensionality reduction.
Consequently, the proposed system can be realized on a field-programmable gate
array (FPGA) device leading to high performance over resource ratio. The
proposed system is tested on real-world event-based datasets for object
categorization, showing superior classification performance and relevance to
state-of-the-art algorithms. Additionally, we verified the object detection
method and real-time FPGA performance in lab settings under non-controlled
illumination conditions with limited training data and ground truth
annotations.Comment: Accepted in ACCV 2018 Workshops, to appea
An encoded N-terminal extension results in low levels of heterologous protein production in Escherichia coli
BACKGROUND: The tdk gene (encoding deoxythymidine kinase) of the gamma-proteobacterium Xenorhabdus nematophila has two potential translation start sites. The promoter-distal start site was predicted to be functional based on amino acid sequence alignment with closely related Tdk proteins. However, to experimentally determine if either of the two possible start codons allows production of a functional Tdk, we expressed the "long-form" (using the promoter-proximal start codon) and "short-form" (using the promoter-distal start codon) X. nematophila tdk genes from the T7 promoter of the pET-28a(+) vector. We assessed Tdk production and activity using a functional assay in an Escherichia coli tdk mutant, which, since it lacks functional Tdk, is able to grow in 5-fluorodeoxyuridine (FUdR)-containing medium. RESULTS: Short-form Tdk complemented the E. coli tdk mutant strain, resulting in FUdR sensitivity of the strain. However, the E. coli tdk mutant expressing the long form of tdk remained FUdR resistant, indicating it did not have a functional deoxythymidine kinase enzyme. We report that long-form Tdk is at least 13-fold less abundant than short-form Tdk, the limited protein produced was as stable as short-form Tdk and the long-form transcript was 1.7-fold less abundant than short-form transcript. Additionally, we report that the long-form extension was sufficient to decrease heterologous production of a different X. nematophila protein, NilC. CONCLUSION: We conclude that the difference in the FUdR growth phenotype between the E. coli tdk mutant carrying the long-or short-form X. nematophila tdk is due to a difference in Tdk levels. The lower long-form protein level does not result from protein instability, but instead from reduced transcript levels possibly combined with reduced translation efficiency. Because the observed effect of the encoded N-terminal extension is not specific to Tdk production and can be overcome with induction of gene expression, these results may have particular relevance to researchers attempting to limit production of toxic proteins under non-inducing conditions
Non-coding RNA regulatory networks
It is well established that the vast majority of human RNA transcripts do not encode for proteins and that non-coding RNAs regulate cell physiology and shape cellular functions. A subset of them is involved in gene regulation at different levels, from epigenetic gene silencing to post-transcriptional regulation of mRNA stability. Notably, the aberrant expression of many non-coding RNAs has been associated with aggressive pathologies. Rapid advances in network biology indicates that the robustness of cellular processes is the result of specific properties of biological networks such as scale-free degree distribution and hierarchical modularity, suggesting that regulatory network analyses could provide new insights on gene regulation and dysfunction mechanisms. In this study we present an overview of public repositories where non-coding RNA-regulatory interactions are collected and annotated, we discuss unresolved questions for data integration and we recall existing resources to build and analyse networks
The virtuoso and the truth lies elsewhere: an encounter with the past through a reading of W.G. Sebald's Austerlitz
The creative project, The Virtuoso , is a novel manuscript loosely based on the last eight years in the life of Australian-born concert pianist Noel Mewton-Wood, who rose to prominence in London in the years during and after the Second World War, before committing suicide in 1953 at the age of thirty-one. The narrator is an unnamed, fictional character who is romantically obsessed with Mewton-Wood, hence his insight into Mewton-Wood and his representation of events is severely coloured by his own desires. Although based on actual events, the manuscript is essentially a study of obsession, fantasy and creativity, and rather than providing an historical record of Mewton-Woodâs life, infers the difficulty in creating such a definitive and objective record. The exegesis, The Truth Lies Elsewhere: An Encounter with the Past through a Reading of W.G. Sebaldâs Austerlitz , is an examination of the techniques W.G. Sebald employs in his novel, Austerlitz , to comment upon the ways we remember our public past; in particular, his use of fiction and narrative strategies to explore memory and outline the problems inherent in the translation of an event into an historical narrative. I argue that by doing this, he not only calls into question the pursuit of a knowable, singular truth in history, but also encourages an alternative approach to âunderstandingâ past events
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Selfâreported sleep patterns and quality amongst adolescents: crossâsectional and prospective associations with anxiety and depression
Background
Sleep problems are common in adolescence, and frequently comorbid with both anxiety and depression. Research studies have suggested a bidirectional relationship between sleep and psychopathology, which includes evidence that sleep interventions can alleviate symptoms of anxiety and depression. However, little is known about the nature of sleep problems amongst adolescents with anxiety and depression, and whether specific sleeping difficulties are involved in the longitudinal relationship between sleep, anxiety and depression.
Method
The sample was derived from the Avon Longitudinal Study of Parents and Children (ALSPAC), a populationâbased, prospective, birth cohort study of children born in 1991â1992. Data were explored from a subset of participants who took part in a clinical assessment at age 15, on selfâreport sleep patterns and quality, and diagnostic outcomes of anxiety and depression (N = 5,033). Subsequent diagnostic and symptom severity data on anxiety and depression at ages 17, 21 and 24 were also examined.
Results
Crossâsectional and longitudinal analyses were conducted to explore the relationship between sleep problems, anxiety and depression. Results revealed that adolescents aged 15 with depression experience difficulties with both sleep patterns and sleep quality, whereas adolescents with anxiety only reported problems with sleep quality. A range of sleep variables at age 15 predicted the severity of anxiety and depression symptoms and the diagnoses of anxiety and depressive disorders at age 17, 21 and 24 years.
Conclusions
The results provide further insight into the nature of sleep problems amongst adolescents with anxiety and depression, and the prospective relationship between sleep disturbance and future psychopathology. These data suggest that targeting sleep difficulties during adolescence may have longâterm mental health benefits
Social and behavioural factors in Non-suspicious unexpected death in infancy; experience from metropolitan police project indigo investigation
BACKGROUND: Risk factors for Sudden Unexpected Death in Infancy (SUDI) are well described, and such cases are now investigated according to standard protocols. In London, Project Indigo of the Metropolitan Police provides a unique, detailed framework for such data collection. We investigate such data to provide a contemporary account of SUDI in a large city and further link data to publically available datasets to investigate interactions with social factors. METHODS: Retrospective analysis of data routinely collected by the Metropolitan Police Service in all cases of non-suspicious SUDI deaths in London during a six year period. RESULTS: SUDI deaths are associated with markers of social deprivation in London. A significant proportion of such deaths are associated with potentially modifiable risk factors such as cigarette smoking and co-sleeping, such behaviour also being associated with social factors, including accommodation issues. CONCLUSIONS: Routinely collected data provide valuable insight into patterns and associations of mortality, with SUDI remaining a significant issue in London. Risk factors include social disadvantage, which may manifest in part by affecting behavioural patterns such as co-sleeping and public health interventions to reduce rates require significant social modification
Coupling of morphology to surface transport in ion-beam irradiated surfaces. I. Oblique incidence
We propose and study a continuum model for the dynamics of amorphizable
surfaces undergoing ion-beam sputtering (IBS) at intermediate energies and
oblique incidence. After considering the current limitations of more standard
descriptions in which a single evolution equation is posed for the surface
height, we overcome (some of) them by explicitly formulating the dynamics of
the species that transport along the surface, and by coupling it to that of the
surface height proper. In this we follow recent proposals inspired by
``hydrodynamic'' descriptions of pattern formation in aeolian sand dunes and
ion-sputtered systems. From this enlarged model, and by exploiting the
time-scale separation among various dynamical processes in the system, we
derive a single height equation in which coefficients can be related to
experimental parameters. This equation generalizes those obtained by previous
continuum models and is able to account for many experimental features of
pattern formation by IBS at oblique incidence, such as the evolution of the
irradiation-induced amorphous layer, transverse ripple motion with non-uniform
velocity, ripple coarsening, onset of kinetic roughening and other.
Additionally, the dynamics of the full two-field model is compared with that of
the effective interface equation.Comment: 23 pages, 14 figures. Movies of figures 6, 7, and 8 available at
http://gisc.uc3m.es/~javier/Movies
Wavelength Tunability of Ion-bombardment Induced Ripples on Sapphire
A study of ripple formation on sapphire surfaces by 300-2000 eV Ar+ ion
bombardment is presented. Surface characterization by in-situ synchrotron
grazing incidence small angle x-ray scattering and ex-situ atomic force
microscopy is performed in order to study the wavelength of ripples formed on
sapphire (0001) surfaces. We find that the wavelength can be varied over a
remarkably wide range-nearly two orders of magnitude-by changing the ion
incidence angle. Within the linear theory regime, the ion induced viscous flow
smoothing mechanism explains the general trends of the ripple wavelength at low
temperature and incidence angles larger than 30. In this model, relaxation is
confined to a few-nm thick damaged surface layer. The behavior at high
temperature suggests relaxation by surface diffusion. However, strong smoothing
is inferred from the observed ripple wavelength near normal incidence, which is
not consistent with either surface diffusion or viscous flow relaxation.Comment: Revtex4, 19 pages, 10 figures with JPEG forma
Mineral sinks within ripening grape berries (Vitis vinifera L.)
Trends in the accumulation of mineral elements into the grape berry components give information about vascular flow into the berry. Shiraz berries were dissected into receptacle, skin, pulp, brush and seeds and the accumulation of 10 mineral elements into these components was followed through development. The elements were separated into two categories according to their accumulation pattern into the berry. The first group of elements continued to accumulate throughout berry growth and ripening, and was comprised of phloem-mobile potassium, phosphorus, sulphur, magnesium, boron, iron and copper. The second group of elements accumulated mostly prior to veraison, and included the xylem-mobile minerals calcium, manganese and zinc. These results indicate that the xylem contribution to berry growth diminished after veraison. Berry fresh weight, dry weight, as well as berry sugar content, were all highly correlated with berry potassium content. While the pulp and skin were the strongest sinks for potassium and boron, seeds were the strongest sinks for calcium, phosphorus, sulphur, manganese and zinc. With the exception of calcium and manganese, seeds ceased to accumulate most elements during late ripening. The berry receptacle and brush did not accumulate any of the elements to levels above those of the other berry components at any stage of development. Therefore, they did not act as sinks for xylem- or phloem-mobile elements as vascular flow to the pulp and skin slowed.
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