Isolation and characterization of a yeast gene, MPD1, the overexpression of which suppresses inviability caused by protein disulfide isomerase depletion

AbstractMPD1, a yeast gene the overexpression of which suppresses the inviability caused by the loss of protein disulfide isomerase (PDI) was isolated and characterized. The MPD1 gene product retained a single disulfide isomerase active site sequence (APWCGHCK), an N-terminal putative signal sequence, and a C-terminal endoplasmic reticulum (ER) retention signal, and was a novel member of the PDI family. The gene product, identified in yeast extract, contained core size carbohydrates. MPD1 was not essential for growth, but overexpression of the gene suppressed the maturation defect of carboxypeptidase Y caused by PDI1 deletion, indicative of the related function to PDI in the yeast ER

EMPRESS. XI. SDSS and JWST Search for Local and z~4-5 Extremely Metal-Poor Galaxies (EMPGs): Clustering and Chemical Properties of Local EMPGs

We search for local extremely metal-poor galaxies (EMPGs), selecting photometric candidates by broadband color excess and machine-learning techniques with the SDSS photometric data. After removing stellar contaminants by shallow spectroscopy with Seimei and Nayuta telescopes, we confirm that three candidates are EMPGs with 0.05--0.1 $Z_\odot$ by deep Magellan/MagE spectroscopy for faint {\sc[Oiii]}$\lambda$4363 lines. Using a statistical sample consisting of 105 spectroscopically-confirmed EMPGs taken from our study and the literature, we calculate cross-correlation function (CCF) of the EMPGs and all SDSS galaxies to quantify environments of EMPGs. Comparing another CCF of all SDSS galaxies and comparison SDSS galaxies in the same stellar mass range ($10^{7.0}-10^{8.4} M_\odot$), we find no significant ($>1\sigma$) difference between these two CCFs. We also compare mass-metallicity relations (MZRs) of the EMPGs and those of galaxies at $z\sim$ 0--4 with a steady chemical evolution model and find that the EMPG MZR is comparable with the model prediction on average. These clustering and chemical properties of EMPGs are explained by a scenario of stochastic metal-poor gas accretion on metal-rich galaxies showing metal-poor star formation. Extending the broadband color-excess technique to a high-$z$ EMPG search, we select 17 candidates of $z\sim$ 4--5 EMPGs with the deep ($\simeq30$ mag) near-infrared JWST/NIRCam images obtained by ERO and ERS programs. We find galaxy candidates with negligible {\sc[Oiii]}$\lambda\lambda$4959,5007 emission weaker than the local EMPGs and known high-$z$ galaxies, suggesting that some of these candidates may fall in 0--0.01 $Z_\odot$, which potentially break the lowest metallicity limit known to date

EMPRESS. II. Highly Fe-Enriched Metal-poor Galaxies with ∼1.0\sim 1.0 (Fe/O)⊙_\odot and 0.020.02 (O/H)⊙_\odot : Possible Traces of Super Massive (>300M⊙>300 M_{\odot}) Stars in Early Galaxies

We present element abundance ratios and ionizing radiation of local young low-mass (~$10^{6}$ M_sun) extremely metal poor galaxies (EMPGs) with a 2% solar oxygen abundance (O/H)_sun and a high specific star-formation rate (sSFR~300 Gyr$^{-1}$), and other (extremely) metal poor galaxies, which are compiled from Extremely Metal-Poor Representatives Explored by the Subaru Survey (EMPRESS) and the literature. Weak emission lines such as [FeIII]4658 and HeII4686 are detected in very deep optical spectra of the EMPGs taken with 8m-class telescopes including Keck and Subaru (Kojima et al. 2019, Izotov et al. 2018), enabling us to derive element abundance ratios with photoionization models. We find that neon- and argon-to-oxygen ratios are comparable to those of known local dwarf galaxies, and that the nitrogen-to-oxygen abundance ratios (N/O) are lower than 20% (N/O)_sun consistent with the low oxygen abundance. However, the iron-to-oxygen abundance ratios (Fe/O) of the EMPGs are generally high; the EMPGs with the 2%-solar oxygen abundance show high Fe/O ratios of ~90-140% (Fe/O)_sun, which are unlikely explained by suggested scenarios of Type Ia supernova iron productions, iron's dust depletion, and metal-poor gas inflow onto previously metal-riched galaxies with solar abundances. Moreover, these EMPGs have very high HeII4686/H$\beta$ ratios of ~1/40, which are not reproduced by existing models of high-mass X-ray binaries whose progenitor stellar masses are less than 120 M_sun. Comparing stellar-nucleosynthesis and photoionization models with a comprehensive sample of EMPGs identified by this and previous EMPG studies, we propose that both the high Fe/O ratios and the high HeII4686/H$\beta$ ratios are explained by the past existence of super massive ($>$300 M_sun) stars, which may evolve into intermediate-mass black holes ($\gtrsim$100 M_sun).Comment: ApJ in press. 23 pages, 7 Figures, 6 Table

Extremely Metal-Poor Representatives Explored by the Subaru Survey (EMPRESS). I. A Successful Machine Learning Selection of Metal-Poor Galaxies and the Discovery of a Galaxy with M*<10^6 M_sun and 0.016 Z_sun

We have initiated a new survey for local extremely metal-poor galaxies (EMPGs) with Subaru/Hyper Suprime-Cam (HSC) large-area (~500 deg^2) optical images reaching a 5 sigma limit of ~26 magnitude, about 100 times deeper than the Sloan Digital Sky Survey (SDSS). To select Z/Z_sun<0.1 EMPGs from ~40 million sources detected in the Subaru images, we first develop a machine-learning (ML) classifier based on a deep neural network algorithm with a training data set consisting of optical photometry of galaxy, star, and QSO models. We test our ML classifier with SDSS objects having spectroscopic metallicity measurements, and confirm that our ML classifier accomplishes 86%-completeness and 46%-purity EMPG classifications with photometric data. Applying our ML classifier to the photometric data of the Subaru sources as well as faint SDSS objects with no spectroscopic data, we obtain 27 and 86 EMPG candidates from the Subaru and SDSS photometric data, respectively. We conduct optical follow-up spectroscopy for 10 out of our EMPG candidates with Magellan/LDSS-3+MagE, Keck/DEIMOS, and Subaru/FOCAS, and find that the 10 EMPG candidates are star-forming galaxies at z=0.007-0.03 with large H_beta equivalent widths of 104-265 A, stellar masses of log(M*/M_sun)=5.0-7.1, and high specific star-formation rates of ~300 Gyr^{-1}, which are similar to those of early galaxies at z>6 reported recently. We spectroscopically confirm that 3 out of 10 candidates are truly EMPGs with Z/Z_sun<0.1, one of which is HSC J1631+4426, the most metal-poor galaxy with Z/Z_sun=0.016 reported ever.Comment: 30 pages, 26 figures, and 8 tables; (Revision: metallicities changed due to the use of a more pricise dust correction method, but conclusion does not change, Minor: a table, some figures, and sentences are added for a clear explanation.