131 research outputs found
Content Analysis of Acculturation Research in Counseling and Counseling Psychology: A 22-Year Review
The authors conducted a 22-year (1988–2009) content analysis of quantitative empirical research that included acculturation and/or enculturation as a study variable(s). A total of 138 studies in 134 articles were systematically evaluated from 5 major American Psychological Association and American Counseling Association journals in counseling and counseling psychology, including Journal of Counseling Psychology, The Counseling Psychologist, Journal of Counseling and Development, Journal of Multicultural Counseling and Development, and Cultural Diversity and Ethnic Minority Psychology. To guide the analysis, the authors conceptualized acculturation/enculturation as a “bilinear” (i.e., developing cultural orientations to both majority and ethnic cultures) and “multidimensional” (i.e., across multiple areas such as behaviors, values, identity, and knowledge) cultural socialization process that occurs in interaction with “social contexts” (e.g., home, school, work, West Coast, Midwest). Findings include the patterns and trends of acculturation/enculturation research in (a) conceptualization and use of acculturation/enculturation variable(s), (b) research designs (e.g., sample characteristics, instruments, data collection, and analysis methods), (c) content areas, and (d) changes in total publications and trends over time. Additionally, meta-analyses were conducted on the relationship of acculturation/enculturation and a few key variables of mental health, adjustment, and well-being. Major findings and directions for future research are discussed
Quantifying the Integration of Quorum-Sensing Signals with Single-Cell Resolution
Cell-to-cell communication in bacteria is a process known as quorum sensing that relies on the production, detection, and response to the extracellular accumulation of signaling molecules called autoinducers. Often, bacteria use multiple autoinducers to obtain information about the vicinal cell density. However, how cells integrate and interpret the information contained within multiple autoinducers remains a mystery. Using single-cell fluorescence microscopy, we quantified the signaling responses to and analyzed the integration of multiple autoinducers by the model quorum-sensing bacterium Vibrio harveyi. Our results revealed that signals from two distinct autoinducers, AI-1 and AI-2, are combined strictly additively in a shared phosphorelay pathway, with each autoinducer contributing nearly equally to the total response. We found a coherent response across the population with little cell-to-cell variation, indicating that the entire population of cells can reliably distinguish several distinct conditions of external autoinducer concentration. We speculate that the use of multiple autoinducers allows a growing population of cells to synchronize gene expression during a series of distinct developmental stages
Hypohidrotic Ectodermal Dysplasia and Immunodeficiency with Coincident NEMO and EDA Mutations
Ectodermal dysplasias (ED) are uncommon genetic disorders resulting in abnormalities in ectodermally derived structures. Many ED-associated genes have been described, of which ectodysplasin-A (EDA) is one of the more common. The NF-κB essential modulator (NEMO encoded by the IKBKG gene) is unique in that mutations result in severe humoral and cellular immunologic defects in addition to ED. We describe three unrelated kindreds with defects in both EDA and IKBKG resulting from X-chromosome crossover. This demonstrates the importance of thorough immunologic consideration of patients with ED even when an EDA etiology is confirmed, and raises the possibility of a specific phenotype arising from coincident mutations in EDA and IKBKG
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The Community Ecology of Herbivore Regulation in an Agroecosystem: Lessons from Complex Systems
AbstractWhether an ecological community is controlled from above or below remains a popular framework that continues generating interesting research questions and takes on especially important meaning in agroecosystems. We describe the regulation from above of three coffee herbivores, a leaf herbivore (the green coffee scale, Coccus viridis), a seed predator (the coffee berry borer, Hypothenemus hampei), and a plant pathogen (the coffee rust disease, caused by Hemelia vastatrix) by various natural enemies, emphasizing the remarkable complexity involved. We emphasize the intersection of this classical question of ecology with the burgeoning field of complex systems, including references to chaos, critical transitions, hysteresis, basin or boundary collision, and spatial self-organization, all aimed at the applied question of pest control in the coffee agroecosystem
PrEP Product Awareness, Preferences, and Past Experiences among Transgender Women and Men Who Have Sex with Men in Asia and Australia: The PrEP APPEAL Study Report
Model binding experiments with cucurbit[7]uril and p-sulfonatocalix[4]arene support use of explicit solvation term in governing equation for binding equilibria
The thermodynamics of model host–guest-binding reactions is examined in depth using isothermal titration calorimetry. In conflict with classical thermodynamics, the results indicate that the equilibrium-binding quotient, K, is not a constant for all pairings. This outcome is predicted by an equation for binding equilibria that includes an explicit term for the change in solvation free energy that accompanies the formation of a binary complex. Application of this framework to the experimentally observed concentration dependence of K allows one to obtain the energetic contribution of the solvent, a linked equilibrium denoted here as ΔGH2O. The estimated values of ΔGH2O are large and unfavourable for the binding of selected guest molecules to two hosts, cucurbit[7]uril and p-sulfonatocalix[4]arene. Intriguingly, the estimated values of ΔGH2O are near zero for the binding of two hydrophobic guest molecules to β-cyclodextrin, leading to a thought-provoking discussion on the driving force behind the hydrophobic effect
Measurement of the copy number of the master quorum-sensing regulator of a bacterial cell
Quorum sensing is the mechanism by which bacteria communicate and synchronize
group behaviors. Quantitative information on parameters such as the copy number
of particular quorum-sensing proteins should contribute strongly to
understanding how the quorum-sensing network functions. Here we show that the
copy number of the master regulator protein LuxR in Vibrio harveyi, can be
determined in vivo by exploiting small-number fluctuations of the protein
distribution when cells undergo division. When a cell divides, both its volume
and LuxR protein copy number N are partitioned with slight asymmetries. We have
measured the distribution functions describing the partitioning of the protein
fluorescence and the cell volume. The fluorescence distribution is found to
narrow systematically as the LuxR population increases while the volume
partitioning is unchanged. Analyzing these changes statistically, we have
determined that N = 80-135 dimers at low cell density and 575 dimers at high
cell density. In addition, we have measured the static distribution of LuxR
over a large (3,000) clonal population. Combining the static and time-lapse
experiments, we determine the magnitude of the Fano factor of the distribution.
This technique has broad applicability as a general, in vivo technique for
measuring protein copy number and burst size.Comment: Main text 23 pages, 5 figures. Supporting material 19 pages, 7
figures. In new version, text revised, one figure reformatte
A Small-Molecule Inhibitor of T. gondii Motility Induces the Posttranslational Modification of Myosin Light Chain-1 and Inhibits Myosin Motor Activity
Toxoplasma gondii is an obligate intracellular parasite that enters cells by a process of active penetration. Host cell penetration and parasite motility are driven by a myosin motor complex consisting of four known proteins: TgMyoA, an unconventional Class XIV myosin; TgMLC1, a myosin light chain; and two membrane-associated proteins, TgGAP45 and TgGAP50. Little is known about how the activity of the myosin motor complex is regulated. Here, we show that treatment of parasites with a recently identified small-molecule inhibitor of invasion and motility results in a rapid and irreversible change in the electrophoretic mobility of TgMLC1. While the precise nature of the TgMLC1 modification has not yet been established, it was mapped to the peptide Val46-Arg59. To determine if the TgMLC1 modification is responsible for the motility defect observed in parasites after compound treatment, the activity of myosin motor complexes from control and compound-treated parasites was compared in an in vitro motility assay. TgMyoA motor complexes containing the modified TgMLC1 showed significantly decreased motor activity compared to control complexes. This change in motor activity likely accounts for the motility defects seen in the parasites after compound treatment and provides the first evidence, in any species, that the mechanical activity of Class XIV myosins can be modulated by posttranslational modifications to their associated light chains
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