N-nitrosodiethylamine induced redox imbalance in rat liver: Protective role of polyphenolic extract of Blighia sapida arilli

a b s t r a c t Aim: This study investigates the reactive oxygen species detoxification potentials of polyphenolic extract of Blighia sapida arilli in the liver of N-nitrosodiethylamine-treated rats. Methods: Male rats, weighing 158 AE 2.9 g were completely randomized into 7 groups (AeG) of five rats each. Rats in C, D, E and F were administered orally once daily at 24 h interval for 7 days with 1000, 250, 500 and 1000 mg/kg body weight of polyphenolic extract of B. sapida arilli respectively. Group G was given 100 mg/kg body weight of vitamin C. On the sixth day, group B, D, E, F and G were administered with 100 mg/kg body weight N-nitrosodiethylamine (NDEA). Group A, which served as the control was treated like the test groups except, that the animals received distilled water only. Results: Reactive oxygen species detoxifying enzymes (superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glucose 6-phosphate dehydrogenase) activities were significantly (P < 0.05) induced by B. sapida arilli. These inductions significantly (P < 0.05) attenuated the decrease in reactive oxygen species detoxifying enzymes mediated by NDEA treatment and compared favourably with vitamin C. NDEA-mediated elevation in the concentrations of oxidative stress biomarkers; malondialdehyde, conjugated dienes, lipid hydroperoxides, protein carbonyl and percentage DNA fragmentation were significantly (P < 0.05) lowered by polyphenolic extract of B. sapida arilli. Conclusion: Overall, the results obtained from this study revealed that the polyphenolic extract from B. sapida arilli enhanced the detoxification of reactive oxygen species in NDEA-treated rats. The polyphenols also prevented the peroxidation of lipid, oxidation of proteins as well as fragmentation of DNA component in the liver of rats and hence gave the evidence of possible prophylactic potentials of B. sapida arilli

Imperatives of Internet and Social Media on Broadcast Journalism in Nigeria

The development of radio and later television brought a new dimension to news and events coverage in Nigeria. In 1993 the monopoly of government was broken to allow private participation.  This gave the broadcast audience multiple choices of media. The advanced technology in the broadcast media and the development of internet and social media networks further exposed the society to alternative mediums of information. The pervasiveness of these websites in our everyday lives is today changing the face of broadcasting globally and in particular the Nigerian broadcast industry. This paper therefore examines the implication of Internet and social media on broadcast journalism in Nigeria. Keywords: Imperative, Internet, social media, broadcast Journalism, Nigeri

EXPLORING SOURSOP KERNEL AS A SUSTAINABLE BIOFUEL: ANALYZING PHYSICAL AND SOLID FLOW PROPERTIES FOR FEASIBILITY ASSESSMENT

  Soursop kernel is an oil-bearing seed containing about 25% non-edible oil per 100 g sample making it a potential feedstock for biofuel production. However, exploring the full potential of the oil requires data to design and fabricate appropriate machines for its processing. So, this necessitates a thorough examination of its physical and solid flow behaviour in relation to moisture contents. The experiments conducted on rewetted samples revealed that as moisture content (mc) increased from 8% to 32.5% dry basis (db), the length, width, thickness, arithmetic and geometric mean diameter, volume, surface area, sphericity, and thousand kernels weight increased from 11.40-12.06 mm, 7.04-7.92 mm, 4.58-5.34 mm, 7.69-8.44 mm, 7.12-7.49 mm, 6.5–8.13 cm3, 160.19-199.05 mm2, 0.62- 0.66 and 186.4–291 g, respectively. The true density decreased from 720 to 670 kg/m³, whereas the bulk density increased from 470 to 570 kg/m³. The angle of repose linearly increased from 25.8° to 39.2° as moisture content increased. The highest mean values for coefficients of friction were observed on mild steel surfaces (0.44 for static and 0.53 for dynamic) at 8.0% mc (db) while the lowest values were recorded on stainless steel surfaces (0.20 for static and 0.37 for dynamic) at both 32.5% and 8.0%, (mc, db). The data obtained will prove beneficial to engineers in the design and development of appropriate machines, as well as other handling and processing equipment such as oil expellers, so as to explore the full industrial application of the oil for biodiesel production and other chemical productio

Maternal exposure to carbon monoxide in the first trimester (7-13+6 weeks) of pregnancy in the core Niger Delta

Background: Irrespective of the fact that the Niger Delta was known for its environmental pollution, neither organized environmental assessment nor human biomonitoring, including that of carbon monoxide (CO) had been performed in the region. The aim of the study therefore was to establish the severity of maternal impact on exposure to CO in the first trimester of pregnancy by quantifying the exhaled CO concentrations (ECOC) and to assess the effect of maternal age, body mass index (BMI) and parity on the severity of the impact.Methods: The study was of cross-sectional design carried out at the Rivers State University Teaching Hospital (RSUTH) in Rivers State. 490 consecutive pregnant women in the first trimester were recruited from the antenatal clinic from January 2021 to January 2022. Gestational age was estimated with the aid of ultrasound scan. Demographic, social and obstetric characteristics were taken. Exhaled carbon monoxide concentration (ECOC) was measured with the aid of a smokerlyzer. Data was analyzed, using the statistical package for social sciences (SPSS) version 25.0 (Armonk, NY) software. Ethical approval was obtained from the RSUTH ethics committee.Results: The mean value of ECOC 3.25±2.51 ppm was more than that obtained in other studies. Out of the total 490 patients that were assessed, 335 (68.37%) had mild impact from CO exposure (ECOC=1-3 ppm), 129 (26.33%) – moderate impact (ECOC=4-6 ppm) and 26 (5.31%) had severe impact (ECOC=more than 6 ppm). Moderate and severe impacts were more prominent in women of age groups 25-39 years and the differences in various age groups were statistically significant [X2=20.671, p<0.036, 95% CI (0.032, 0.040)]. Patients with higher BMI were more likely to have moderate and severe impact than those with lower BMI- 6 (33.33%) and 4 (22.22%) out of the 18 patients with class III obesity had moderate and severe impacts respectively. The differences in the impact at various BMI were statistically significant [X2=20.671, p<0.001, 95% CI (0.001, 0.002)]. There was inverse relationship between parity and the severity of the impact and the differences in various parity groups were statistically significant [X2=10.580, p<0.012, 95% CI (0.101, 0.113)]. There was also a paradoxical finding of 3 smokers having only mild impact.Conclusions: The mean value of ECOC 3.25±2.51 ppm was more than that obtained in other studies in non-pregnant women. Mild, moderate and severe impact from maternal CO exposure were established with the moderate and severe impact more prominent at maternal ages of 25-39 years, at higher BMI and at lower parity

Liver mitochondrial membrane permeability modulation in insulin-resistant, uninephrectomised male rats by Clerodendrum volubile P. Beauv and Manihot esculenta Crantz

Background: Non-alcoholic fatty liver disease, which occurs in people who are not alcohol drinkers, describes some of the pathogenic conditions that may be in the least characterized by simple steatosis or can be as serious as non-alcoholic steatohepatitis and cirrhosis. Its mechanistic pathogenesis has been said to arise from insulin resistance and oxidative stress, which may be compounded by obesity. An experimental model showing, systemic insulin resistance, obesity and accumulated hepatic fatty acids was created in adult male rats using high-fat diet manipulation and surgical removal of the left kidney (uninephrectomy). This study sought to identify the impact of these multiple burdens on the liver mitochondrial membrane permeability transition pore opening, and the possible in vitro effects of the extracts of Clerodendrum volubile and Manihot esculenta leaves on the membrane permeabilization. Results: The results indicated that the methanolic extract of Clerodendrum volubile leaf inhibited mitochondrial membrane pore opening in the insulin resistance condition or when it is followed by uni-nephrectomy, while the ethanolic extract of Manihot esculenta leaf does the same in the insulin resistance condition both prior to and following uni-nephrectomy. Conclusion: Since the vegetable extracts were able to abrogate mitochondrial pore opening at low concentrations, the structural integrity of the mitochondria can possibly be restored over time if treated by the vegetable extracts. Research efforts should, therefore, be made to harness the drugability of the bioactives of these vegetables for use in the treatment of non-alcoholic fatty liver disease arising from insulin resistance and renal failure.Fil: Ajayi, Ebenezer Idowu O. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. Osun State University; Nigeria. National Institute of Pharmaceutical Education and Research; IndiaFil: Molehin, Olorunfemi R.. Ekiti State University; NigeriaFil: Oloyede, Omotade I.. Ekiti State University; NigeriaFil: Kumar, Vinodu. National Institute of Pharmaceutical Education and Research; IndiaFil: Amara, Venkateswara R.. National Institute of Pharmaceutical Education and Research; IndiaFil: Kaur, Jasmine. National Institute of Pharmaceutical Education and Research; IndiaFil: Karpe, Pinakin. National Institute of Pharmaceutical Education and Research; IndiaFil: Tikoo, Kulbhushan B.. National Institute of Pharmaceutical Education and Research; Indi

Transforming Nigeria Food System: The Role of Agroforestry

It has been generally established that the global food system needs to be transformed urgently, in order to meet the growing demand for food, feed and fodder. Agroforestry is a type of farming system that combines trees/shrubs with crops with the objective of enjoying a mutual benefit that enhances productivity, profitability and biodiversity, which at the moment is unpopular in Nigeria. Therefore the objective of this paper is to provide relevant information on the role of agroforestry in transforming the Nigerian food system. Some of the agroforestry technologies which can be used to achieve its mutual benefit include taungya system, home garden, alley cropping, improved fallow, woodlots, live fences, fodder banks, orchards or tree gardens, windbreaks, shelterbelts, trees on pasture and apiculture with trees. Planting trees as a component of crop farming is a smart way to achieve multiple purposes with a single enterprise. Planting multipurpose trees in a world affected by climate change can decelerate the negative impact of anthropogenic cause of climate change, while providing food for humanity. Hence, the paper also review the roles of agroforestry in the global fight against climate change, while also ensuring food security through food system transformation

CLEAR – clozapine in early psychosis: study protocol for a multi-centre, randomised controlled trial of clozapine vs other antipsychotics for young people with treatment resistant schizophrenia in real world settings

Background: Clozapine is an antipsychotic drug with unique efficacy, and it is the only recommended treatment for treatment-resistant schizophrenia (TRS: failure to respond to at least two different antipsychotics). However, clozapine is also associated with a range of adverse effects which restrict its use, including blood dyscrasias, for which haematological monitoring is required. As treatment resistance is recognised earlier in the illness, the question of whether clozapine should be prescribed in children and young people is increasingly important. However, most research to date has been in older, chronic patients, and evidence regarding the efficacy and safety of clozapine in people under age 25 is lacking. The CLEAR (CLozapine in EARly psychosis) trial will assess whether clozapine is more effective than treatment as usual (TAU), at the level of clinical symptoms, patient rated outcomes, quality of life and cost-effectiveness in people below 25 years of age. Additionally, a nested biomarker study will investigate the mechanisms of action of clozapine compared to TAU. Methods and design: This is the protocol of a multi-centre, open label, blind-rated, randomised controlled effectiveness trial of clozapine vs TAU (any other oral antipsychotic monotherapy licenced in the British National Formulary) for 12 weeks in 260 children and young people with TRS (12–24 years old). Aim and objectives: The primary outcome is the change in blind-rated Positive and Negative Syndrome Scale scores at 12 weeks from baseline. Secondary outcomes include blind-rated Clinical Global Impression, patient-rated outcomes, quality of life, adverse effects, and treatment adherence. Patients will be followed up for 12 months and will be invited to give consent for longer term follow-up using clinical records and potential re-contact for further research. For mechanism of action, change in brain magnetic resonance imaging (MRI) biomarkers and peripheral inflammatory markers will be measured over 12 weeks. Discussion: The CLEAR trial will contribute knowledge on clozapine effectiveness, safety and cost-effectiveness compared to standard antipsychotics in young people with TRS, and the results may guide future clinical treatment recommendation for early psychosis. Trial registration: ISRCTN Number: 37176025, IRAS Number: 1004947. Trial status: In set-up. Protocol version 4.0 01/08/23. Current up to date protocol available here: https://fundingawards.nihr.ac.uk/award/NIHR131175#/