Algal culture studies related to a Closed Ecological Life Support System (CELSS)

Studies with algal cultures which relate to closed ecological life support systems (CELSS) are discussed. A description of a constant cell density apparatus for continuous culture of algae is included. Excretion of algal by-products, and nitrogen utilization and excretion are discussed

Food Safety Audits, Plant Characteristics, and Food Safety Technology Use in Meat and Poultry Plants

Food safety technology can increase a company’s capacity to prevent a foodborne contamination. A food safety audit—a quality control tool in which an auditor observes whether a plant’s processing practices and technologies are compatible with good food safety practices—can indicate how effectively food safety technology is being used. Fast food restaurants, grocery stores, and other major customers of meat and poultry processing plants conduct their own audits or hire auditors to assess the soundness of a plant’s processing operation. Meat and poultry plants can also audit themselves as a way to help maintain process control. In this report, we document the extent of food safety audits in meat and poultry processing plants. We also examine the associations between the use of audits and plant size, firm structure, and food safety technology use. Results show that larger plants, plants subject to food safety audits, and plants that are part of a multiplant firm use more food safety technology than other plants. Plants subject to both plant-hired and customer-hired audits had greater technology use than single (plant- or customer-hired) audit plants.Meat and poultry processing, safety standards, product recalls, food safety technology, food safety audits, Agribusiness, Food Consumption/Nutrition/Food Safety, Industrial Organization, Livestock Production/Industries,

Quantifying the effects of harvesting on carbon fluxes and stocks in northern temperate forests

Harvest disturbance has substantial impacts on forest carbon (C) fluxes and stocks. The quantification of these effects is essential for the better understanding of forest C dynamics and informing forest management in the context of global change. We used a process-based forest ecosystem model, PnET-CN, to evaluate how, and by what mechanisms, clear-cuts alter ecosystem C fluxes, aboveground C stocks (AGC), and leaf area index (LAI) in northern temperate forests. We compared C fluxes and stocks predicted by the model and observed at two chronosequences of eddy covariance flux sites for deciduous broadleaf forests (DBF) and evergreen needleleaf forests (ENF) in the Upper Midwest region of northern Wisconsin and Michigan, USA. The average normalized root mean square error (NRMSE) and the Willmott index of agreement (d) for carbon fluxes, LAI, and AGC in the two chronosequences were 20% and 0.90, respectively. Simulated gross primary productivity (GPP) increased with stand age, reaching a maximum (1200–1500 g C m−2 yr−1) at 11–30 years of age, and leveled off thereafter (900–1000 g C m−2 yr−1). Simulated ecosystem respiration (ER) for both plant functional types (PFTs) was initially as high as 700–1000 g C m−2 yr−1 in the first or second year after harvesting, decreased with age (400–800 g C m−2 yr−1) before canopy closure at 10–25 years of age, and increased to 800–900 g C m−2 yr−1 with stand development after canopy recovery. Simulated net ecosystem productivity (NEP) for both PFTs was initially negative, with net C losses of 400–700 g C m−2 yr−1 for 6–17 years after clear-cuts, reaching peak values of 400–600 g C m−2 yr−1 at 14–29 years of age, and eventually stabilizing in mature forests (\u3e 60 years old), with a weak C sink (100–200 g C m−2 yr−1). The decline of NEP with age was caused by the relative flattening of GPP and gradual increase of ER. ENF recovered more slowly from a net C source to a net sink, and lost more C than DBF. This suggests that in general ENF may be slower to recover to full C assimilation capacity after stand-replacing harvests, arising from the slower development of photosynthesis with stand age. Our model results indicated that increased harvesting intensity would delay the recovery of NEP after clear-cuts, but this had little effect on C dynamics during late succession. Future modeling studies of disturbance effects will benefit from the incorporation of forest population dynamics (e.g., regeneration and mortality) and relationships between age-related model parameters and state variables (e.g., LAI) into the model

On Factor Universality in Symbolic Spaces

The study of factoring relations between subshifts or cellular automata is central in symbolic dynamics. Besides, a notion of intrinsic universality for cellular automata based on an operation of rescaling is receiving more and more attention in the literature. In this paper, we propose to study the factoring relation up to rescalings, and ask for the existence of universal objects for that simulation relation. In classical simulations of a system S by a system T, the simulation takes place on a specific subset of configurations of T depending on S (this is the case for intrinsic universality). Our setting, however, asks for every configurations of T to have a meaningful interpretation in S. Despite this strong requirement, we show that there exists a cellular automaton able to simulate any other in a large class containing arbitrarily complex ones. We also consider the case of subshifts and, using arguments from recursion theory, we give negative results about the existence of universal objects in some classes

Positron-Emission Tomography

We review positron-emission tomography (PET), which has inherent advantages that avoid the shortcomings of other nuclear medicine imaging methods. PET image reconstruction methods with origins in signal and image processing are discussed, including the potential problems of these methods. A summary of statistical image reconstruction methods, which can yield improved image quality, is also presented.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/85853/1/Fessler95.pd

An admittance shaping controller for exoskeleton assistance of the lower extremities

We present a method for lower-limb exoskeleton control that defines assistance as a desired dynamic response for the human leg. Wearing the exoskeleton can be seen as replacing the leg's natural admittance with the equivalent admittance of the coupled system. The control goal is to make the leg obey an admittance model defined by target values of natural frequency, peak magnitude and zero-frequency response. No estimation of muscle torques or motion intent is necessary. Instead, the controller scales up the coupled system's sensitivity transfer function by means of a compensator employing positive feedback. This approach increases the leg's mobility and makes the exoskeleton an active device capable of performing net positive work on the limb. Although positive feedback is usually considered destabilizing, here performance and robust stability are successfully achieved through a constrained optimization that maximizes the system's gain margins while ensuring the desired location of its dominant poles

Integral admittance shaping: A unified framework for active exoskeleton control

© 2015 Elsevier B.V. Current strategies for lower-limb exoskeleton control include motion intent estimation, which is subject to inaccuracies in muscle torque estimation as well as modeling error. Approaches that rely on the phases of a uniform gait cycle have proven effective, but lack flexibility to aid other kinds of movement. This research aims at developing a more versatile control that can assist the lower limbs independently of the movement attempted. Our control strategy is based on modifying the dynamic response of the human limbs, specifically their mechanical admittance. Increasing the admittance makes the lower limbs more responsive to any muscle torque generated by the human user. We present Integral Admittance Shaping, a unified mathematical framework for: (a) determining the desired dynamic response of the coupled system formed by the human limb and the exoskeleton, and (b) synthesizing an exoskeleton controller capable of achieving said response. The present control formulation focuses on single degree-of-freedom exoskeleton devices providing performance augmentation. The algorithm generates a desired shape for the frequency response magnitude of the integral admittance (torque-to-angle relationship) of the coupled system. Simultaneously, it generates an optimal feedback controller capable of achieving the desired response while guaranteeing coupled stability and passivity. The potential effects of the exoskeleton's assistance are motion amplification for the same joint torque, and torque reduction for the same joint motion. The robustness of the derived exoskeleton controllers to parameter uncertainties is analyzed and discussed. Results from initial trials using the controller on an experimental exoskeleton are presented as well

Integral Admittance Shaping for Exoskeleton Control

A wide variety of strategies have been developed for assisting human locomotion using powered exoskeletons. Although these strategies differ in their aims as well as the control methods employed, they have the implicit property of causing a virtual modification of the dynamic response of the human limb. We use this property of the exoskeletons action to formulate a unified control design framework called Integral Admittance Shaping, which designs exoskeleton controllers capable of producing the desired dynamic response for the assisted limb. In this framework, a virtual increase in the admittance of the limb is produced by coupling it to an exoskeleton that exhibits active behavior. Specifically, our framework shapes the magnitude profile of the integral admittance (i.e. torque-to-angle relationship) of the coupled human-exoskeleton system, such that the desired assistance is achieved. This framework also ensures that the coupled stability and passivity are guaranteed. This paper presents a formulation of Integral Admittance Shaping for single degree-of-freedom (1-DOF) exoskeleton devices. We also present experimental results on a modified version of Honda’s Stride Management Assist (SMA) device that successfully demonstrate motion amplification of the assisted hip joint during walking

A Dual Read-Out Assay to Evaluate the Potency of Compounds Active against Mycobacterium tuberculosis

PMCID: PMC3617142This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

DIRECT ESTIMATION OF ABOVEGROUND FOREST PRODUCTIVITY THROUGH HYPERSPECTRAL REMOTE SENSING OF CANOPY NITROGEN

The concentration of nitrogen in foliage has been related to rates of net photosynthesis across a wide range of plant species and functional groups and thus represents a simple and biologically meaningful link between terrestrial cycles of carbon and nitrogen. Although foliar N is used by ecosystem models to predict rates of leaf‐level photosynthesis, it has rarely been examined as a direct scalar to stand‐level carbon gain. Establishment of such relationships would greatly simplify the nature of forest C and N linkages, enhancing our ability to derive estimates of forest productivity at landscape to regional scales. Here, we report on a highly predictive relationship between whole‐canopy nitrogen concentration and aboveground forest productivity in diverse forested stands of varying age and species composition across the 360 000‐ha White Mountain National Forest, New Hampshire, USA. We also demonstrate that hyperspectral remote sensing can be used to estimate foliar N concentration, and hence forest production across a large number of contiguous images. Together these data suggest that canopy‐level N concentration is an important correlate of productivity in these forested systems, and that imaging spectrometry of canopy N can provide direct estimates of forest productivity across large landscapes