Conservation and trade of the endangered Hypancistrus zebra (Siluriformes, Loricariidae), one of the most trafficked Brazilian fish

Abstract Hypancistrus zebra, also known as the zebra pleco, is a small sucker-mouth catfish endemic to the Xingu River in Brazil where its survival in the wild is threatened by habitat modification and overfishing for the ornamental fish industry. It is a highly sought-after freshwater ornamental species and one of the most commonly trafficked from Brazil. To date, little is known about its global legal and illicit supply chains within the ornamental fish trade. Through a mixed methods approach (i.e., online survey, key informant interviews and web scraping), we examined the trade and trafficking of this species as well as the awareness of the international aquarist community and local and international stakeholders regarding its conservation. We also establish the historical timeline of zebra pleco keeping and breeding in captivity and assess whether commercial captive breeding can play an important role in the conservation of this species. The retail price of the zebra pleco increased worldwide after an export ban in 2004 but have since decreased to an average of $US 155 (+/-$US 23 based on geographical location) per fish. Fishermen have been consistently paid relatively little ($US 7–60) for each specimen compared to the average wholesale price of$US 100 (+/- $US 94 over time). We conservatively estimate ~100,000 specimens are trafficked out of Brazil annually, of which half or more die in transport, and only a small fraction is seized by law enforcement in Brazil or internationally. The fishes are primarily smuggled from Brazil to Peru and Colombia and then exported internationally with the majority sent to China. The majority of aquarists surveyed (representing 35 countries) were aware the zebra pleco is both endangered and highly endemic. There was less awareness that buying wild caught specimens shipped from Peru, Colombia or elsewhere implies supporting wildlife trafficking. Nevertheless, nearly three quarters of respondents preferred aquarium bred specimens, if available. The zebra pleco is being bred in captivity in high numbers in several countries, yet in Brazil it remains illegal to keep in private aquaria or to commercially breed them. Given the large success of hobby and commercial breeders around the world, H. zebra is well suited for indoor breeding facilities. We argue that implementing regulated local breeding facilities in Brazil to increase the already large numbers reproduced in captivity worldwide, could decrease the demand for trafficked specimens, one of the primary factors threatening its survival. Given its iconic status among freshwater fishes it should be recognized as a flagship species of the Xingu River's conservation

Assessing the impact of illumination on UAV pushbroom hyperspectral imagery collected under various cloud cover conditions

The recent development of small form-factor (<6 kg), full range (400–2500 nm) pushbroom hyperspectral imaging systems (HSI) for unmanned aerial vehicles (UAV) poses a new range of opportunities for passive remote sensing applications. The flexible deployment of these UAV-HSI systems have the potential to expand the data acquisition window to acceptable (though non-ideal) atmospheric conditions. This is an important consideration for time-sensitive applications (e.g. phenology) in areas with persistent cloud cover. Since the majority of UAV studies have focused on applications with ideal illumination conditions (e.g. minimal or non-cloud cover), little is known to what extent UAV-HSI data are affected by changes in illumination conditions due to variable cloud cover. In this study, we acquired UAV pushbroom HSI (400–2500 nm) over three consecutive days with various illumination conditions (i.e. cloud cover), which were complemented with downwelling irradiance data to characterize illumination conditions and in-situ and laboratory reference panel measurements across a range of reflectivity (i.e. 2%, 10%, 18% and 50%) used to evaluate reflectance products. Using these data we address four fundamental aspects for UAV-HSI acquired under various conditions ranging from high (624.6 ± 16.63 W·m2) to low (2.5 ± 0.9 W·m2) direct irradiance: atmospheric compensation, signal-to-noise ratio (SNR), spectral vegetation indices and endmembers extraction. For instance, two atmospheric compensation methods were applied, a radiative transfer model suitable for high direct irradiance, and an Empirical Line Model (ELM) for diffuse irradiance conditions. SNR results for two distinctive vegetation classes (i.e. tree canopy vs herbaceous vegetation) reveal wavelength dependent attenuation by cloud cover, with higher SNR under high direct irradiance for canopy vegetation. Spectral vegetation index (SVIs) results revealed high variability and index dependent effects. For example, NDVI had significant differences (p < 0.05) across illumination conditions, while NDWI appeared insensitive at the canopy level. Finally, often neglected diffuse illumination conditions may be beneficial for revealing spectral features in vegetation that are obscured by the predominantly non-Lambertian reflectance encountered under high direct illumination. To our knowledge, our study is the first to use a full range pushbroom UAV sensor (400–2500 nm) for assessing illumination effects on the aforementioned variables. Our findings pave the way for understanding the advantages and limitations of ultra-high spatial resolution full range high fidelity UAV-HSI for ecological and other applications

Comparing UAS LiDAR and Structure-from-Motion Photogrammetry for Peatland Mapping and Virtual Reality (VR) Visualization

The mapping of peatland microtopography (e.g., hummocks and hollows) is key for understanding and modeling complex hydrological and biochemical processes. Here we compare unmanned aerial system (UAS) derived structure-from-motion (SfM) photogrammetry and LiDAR point clouds and digital surface models of an ombrotrophic bog, and we assess the utility of these technologies in terms of payload, efficiency, and end product quality (e.g., point density, microform representation, etc.). In addition, given their generally poor accessibility and fragility, peatlands provide an ideal model to test the usability of virtual reality (VR) and augmented reality (AR) visualizations. As an integrated system, the LiDAR implementation was found to be more straightforward, with fewer points of potential failure (e.g., hardware interactions). It was also more efficient for data collection (10 vs. 18 min for 1.17 ha) and produced considerably smaller file sizes (e.g., 51 MB vs. 1 GB). However, SfM provided higher spatial detail of the microforms due to its greater point density (570.4 vs. 19.4 pts/m2). Our VR/AR assessment revealed that the most immersive user experience was achieved from the Oculus Quest 2 compared to Google Cardboard VR viewers or mobile AR, showcasing the potential of VR for natural sciences in different environments. We expect VR implementations in environmental sciences to become more popular, as evaluations such as the one shown in our study are carried out for different ecosystems

High-Resolution Surface Water Classifications of the Xingu River, Brazil, Pre and Post Operationalization of the Belo Monte Hydropower Complex

We describe a new high spatial resolution surface water classification dataset generated for the Xingu river, Brazil, from its confluence with the Iriri river to the Pimental dam prior to construction of the Belo Monte hydropower complex, and after its operationalization. This river is well-known for its exceptionally high diversity and endemism in ichthyofauna. Pre-existing datasets generated from moderate resolution satellite imagery (e.g., 30 m) do not adequately capture the extent of the river. Accurate measurements of water extent are important for a range of applications utilizing surface water data, including greenhouse gas emission estimation, land cover change mapping, and habitat loss/change estimates, among others. We generated the new classifications from RapidEye imagery (5 m pixel size) for 2011 and PlanteScope imagery (3 m pixel size) for 2019 using a Geographic Object Based Image Analysis (GEOBIA) approach

A New Multi-Temporal Forest Cover Classification for the Xingu River Basin, Brazil

We describe a new multi-temporal classification for forest/non-forest classes for a 1.3 million square kilometer area encompassing the Xingu River basin, Brazil. This region is well known for its exceptionally high biodiversity, especially in terms of the ichthyofauna, with approximately 600 known species, 10% of which are endemic to the river basin. Global and regional scale datasets do not adequately capture the rapidly changing land cover in this region. Accurate forest cover and forest cover change data are important for understanding the anthropogenic pressures on the aquatic ecosystems. We developed the new classifications with a minimum mapping unit of 0.8 ha from cloud free mosaics of Landsat TM5 and OLI 8 imagery in Google Earth Engine using a classification and regression tree (CART) aided by field photographs for the selection of training and validation points

Assessment of UAS Photogrammetry and Planet Imagery for Monitoring Water Levels around Railway Tracks

High water levels near railway tracks can be a major factor affecting the safety of train passage. Water conditions near the tracks are normally monitored through visual inspections. However, this method is limited in spatial coverage and may not provide comparable information over time. We evaluated the utility of satellite imagery (Planet Dove constellation at 3 m pixel size) at the landscape level to assess overall water surface area along railway tracks. Comparatively, we evaluated the use of Structure- from-Motion 3D point clouds and high spatial detail orthomosaics (3 cm) generated from a commercial off-the-shelf Unmanned Aerial System (UAS) (DJI M300 RTK) for measuring vertical water level changes and extent of surface water, respectively, within the right-of-way of a railway line in Ontario, Canada, in areas prone to high water level and flooding. Test sites of varied lengths (~180 m to 500 m), were assessed four times between June and October 2021. Our results indicate that the satellite imagery provides a large-scale overview regarding the extent of open water in wetlands at long distances from the railway tracks. Analysis of the UAS derived 3D point cloud indicates that changes in water level can be determined at the centimeter scale. Furthermore, the spatial error (horizontal and vertical alignments) between the multi-temporal UAS data collections between sites was less than 3 cm. Our research highlights the importance of using consistent UAS data collection protocols, and the significant potential of commercial off-the-shelf UAS systems for water level monitoring along railway tracks

Land Cover, Land Use, and Climate Change Impacts on Endemic Cichlid Habitats in Northern Tanzania

Freshwater ecosystems are among the most threatened on Earth, facing environmental and anthropogenic pressures often surpassing their terrestrial counterparts. Land use and land cover change (LUCC) such as degradation and fragmentation of the terrestrial landscape negatively impacts aquatic ecosystems. Satellite imagery allows for an impartial assessment of the past to determine habitat alterations. It can also be used as a forecasting tool in the development of species conservation strategies through models based on ecological factors extracted from imagery. In this study, we analyze Landsat time sequences (1984–2015) to quantify LUCC around three freshwater ecosystems with endemic cichlids in Tanzania. In addition, we examine population growth, agricultural expansion, and climate change as stressors that impact the habitats. We found that the natural vegetation cover surrounding Lake Chala decreased from 15.5% (1984) to 3.5% (2015). At Chemka Springs, we observed a decrease from 7.4% to 3.5% over the same period. While Lake Natron had minimal LUCC, severe climate change impacts have been forecasted for the region. Subsurface water data from the Gravity Recovery and Climate Experiment (GRACE) satellite observations further show a decrease in water resources for the study areas, which could be exacerbated by increased need from a growing population and an increase in agricultural land use

UAV-Based 3D Point Clouds of Freshwater Fish Habitats, Xingu River Basin, Brazil

Dense 3D point clouds were generated from Structure-from-Motion Multiview Stereo (SFM-MVS) photogrammetry for five representative freshwater fish habitats in the Xingu river basin, Brazil. The models were constructed from Unmanned Aerial Vehicle (UAV) photographs collected in 2016 and 2017. The Xingu River is one of the primary tributaries of the Amazon River. It is known for its exceptionally high aquatic biodiversity. The dense 3D point clouds were generated in the dry season when large areas of aquatic substrate are exposed due to the low water level. The point clouds were generated at ground sampling distances of 1.20&ndash;2.38 cm. These data are useful for studying the habitat characteristics and complexity of several fish species in a spatially explicit manner, such as calculation of metrics including rugosity and the Minkowski&ndash;Bouligand fractal dimension (3D complexity). From these dense 3D point clouds, substrate complexity can be determined more comprehensively than from conventional arbitrary cross sections

A Practical Validation of Uncooled Thermal Imagers for Small RPAS

Uncooled thermal imaging sensors in the LWIR (7.5 μm to 14 μm) have recently been developed for use with small RPAS. This study derives a new thermal imaging validation methodology via the use of a blackbody source (indoors) and real-world field conditions (outdoors). We have demonstrated this method with three popular LWIR cameras by DJI (Zenmuse XT-R, Zenmuse XT2 and, the M2EA) operated by three different popular DJI RPAS platforms (Matrice 600 Pro, M300 RTK and, the Mavic 2 Enterprise Advanced). Results from the blackbody work show that each camera has a highly linearized response (R2 > 0.99) in the temperature range 5–40 °C as well as a small (<2 °C) temperature bias that is less than the stated accuracy of the cameras. Field validation was accomplished by imaging vegetation and concrete targets (outdoors and at night), that were instrumented with surface temperature sensors. Environmental parameters (air temperature, humidity, pressure and, wind and gusting) were measured for several hours prior to imaging data collection and found to either not be a factor, or were constant, during the ~30 min data collection period. In-field results from imagery at five heights between 10 m and 50 m show absolute temperature retrievals of the concrete and two vegetation sites were within the specifications of the cameras. The methodology has been developed with consideration of active RPAS operational requirements

A Practical Validation of Uncooled Thermal Imagers for Small RPAS

Uncooled thermal imaging sensors in the LWIR (7.5 μm to 14 μm) have recently been developed for use with small RPAS. This study derives a new thermal imaging validation methodology via the use of a blackbody source (indoors) and real-world field conditions (outdoors). We have demonstrated this method with three popular LWIR cameras by DJI (Zenmuse XT-R, Zenmuse XT2 and, the M2EA) operated by three different popular DJI RPAS platforms (Matrice 600 Pro, M300 RTK and, the Mavic 2 Enterprise Advanced). Results from the blackbody work show that each camera has a highly linearized response (R2 &gt; 0.99) in the temperature range 5–40 °C as well as a small (&lt;2 °C) temperature bias that is less than the stated accuracy of the cameras. Field validation was accomplished by imaging vegetation and concrete targets (outdoors and at night), that were instrumented with surface temperature sensors. Environmental parameters (air temperature, humidity, pressure and, wind and gusting) were measured for several hours prior to imaging data collection and found to either not be a factor, or were constant, during the ~30 min data collection period. In-field results from imagery at five heights between 10 m and 50 m show absolute temperature retrievals of the concrete and two vegetation sites were within the specifications of the cameras. The methodology has been developed with consideration of active RPAS operational requirements