Molecular Detection of Torque Teno Sus Virus and Coinfection with African Swine Fever Virus in Blood Samples of Pigs from Some Slaughterhouses in Nigeria

Torque teno sus virus 1 (TTSuV1a/TTSuV1b) infection is present in pig herds worldwide. This study investigated the prevalence of TTSuV1a/TTSuV1b infections in domestic pigs from some slaughterhouses in Nigeria as well as coinfection with African swine fever virus (ASFV) and described the phylogeny in relation to global strains. One hundred and eighty-one (181) blood samples from four slaughterhouses were used for the study and viral nucleic acid detection was carried out by PCR. Comparative sequence analysis was carried out to infer phylogeny. The overall prevalence of TTSuV1a/b was 17.7%. Prevalence of individual genotypes was 10.5% and 7.2% for TTSuV1a and TTSuV1b, respectively. Coinfection of ASFV/TTSuV1a/b was 7.7% while that of TTSuV1a and TTSuV1b was 1.7%. ASFV alone was detected in 11.91% of the total samples. The Nigerian TTSuV1a and TTSuV1b shared a sequence identity of 91–100% and 95–100%, respectively, among each other. The ASFV sequences were 100% identical to members of genotype 1. This is the first report on the presence of TTSuV1a/b in domestic pigs in Nigeria and coinfection with ASFV. Although the prevalence of TTSuV1a/b in Nigeria was low, we recommend further studies to establish the trend and possible role in the pathogenesis of ASFV

Seroprevalence of Influenza A Virus in Dromedaries in North-Western Nigeria

Although influenza A virus is endemic in wild waterfowl, domestic poultry, swine, humans, bats, cetaceans, dogs, and horses, there is a paucity of data on the potential role of camels in zoonotic transmission of the virus. To estimate the seroprevalence of the influenza A virus in camel populations, four local government areas of Nigeria that share an international border with the Niger Republic were selected. Blood samples from 184 one-hump camels (dromedaries) were collected and tested for influenza IgG antigen by ELISA. Each camel’s demographic variable, such as age, gender, location, production system, and usage, was recorded. The overall seroprevalence rate of influenza virus IgG in this study was 10.33% (95%CI: 6.33–15.66%). In the bivariate model, there was no significant difference in gender, age, site location and production system, except for usage. There was a significantly lower seroprevalence rate among camels used for labour (odds ratio (OR) = 0.34, 95% CI: 0.10–0.97) than those used for meat consumption; however, not after adjusting for other variables in the model. Increase surveillance through early detection, prediction, and risk assessment of pathogens in animal reservoirs and environmental contamination as One Health strategies to reduce potential human spillover is recommended. Molecular epidemiology studies could better elucidate the role of camels in the dynamics of disease transmission pathways

First Report of Hepatitis E Virus Circulation in Domestic Pigs in Nigeria

Hepatitis E, a usually self-limiting but occasionally fulminant hepatitis is an important public health concern in low- and middle-income countries worldwide.1 Hepatitis E is the single most important cause of acute clinical hepatitis among adults throughout Central and Southeast Asia and the second most important cause throughout the Middle East and North Africa following hepatitis B.2 Models derived from epidemiologic and clinical studies project a conservative estimate of the annual impact of hepatitis E to include ∼3.4 million symptomatic cases, 70,000 deaths, and 3,000 stillbirths.3 The etiologic agent of hepatitis E is hepatitis E virus (HEV),4 classified as a member of the genus Hepevirus, family Hepeviridae.5 The virus is a non-enveloped, single-stranded positive-sense RNA virus that contains three open reading frames (ORF); ORF1 encodes for non-structural proteins, ORF2 encodes for the capsid protein, and ORF3 encodes for a multi-functional small protein.5 Currently, HEV has at least four recognized genotypes capable of infecting humans.5 The HEV genotypes 1 and 2 are restricted to humans and related to waterborne large outbreaks of hepatitis E in developing countries with poor sanitation conditions.6 Genotype 1 is primarily found in North Africa and Central and South Asia.7 Genotype 2 circulates in Mexico and West Africa.7 Genotype 3 circulates worldwide and contains strains from sporadic, cluster, and chronic cases of hepatitis E in humans6 and strains from pigs and wild boars, which are considered the most important reservoirs for HEV.8 Genotype 4 circulates mainly in Asia; however, it has been reported recently in Europe9–11 and includes strains from sporadic and cluster cases of hepatitis E in humans and strains from pigs. In Africa, HEV is still an emerging pathogen. The very low rate of reporting is the result of suboptimal investigations and neglect of the disease and its public health problem. The majority of available reports describe human HEV outbreaks in North Africa and there are few reports from Uganda, the Central African Republic, the Democratic Republic of the Congo, Sudan, South Sudan and Kenya.1 Available information on HEV infection in animals in Africa is very limited. Two reports describe detection of HEV in pigs in the Democratic Republic of the Congo and in Cameroon,12,13 indicating increased awareness, surveillance, and widespread nature of the virus in the environment. The only available characterized isolate of a human HEV case in Nigeria belongs to genotype 2.14 This particular genotype is commonly linked to human outbreaks. As in other African countries, the HEV infectious status of pigs in Nigeria is largely unknown. The objective of this study was to characterize HEV infection in pigs of various ages from the main pig farming regions in Nigeria.This article is published as Owolodun, Olajide A., Priscilla F. Gerber, Luis G. Giménez-Lirola, Jacob KP Kwaga, and Tanja Opriessnig. "First report of hepatitis E virus circulation in domestic pigs in Nigeria." The American Journal of Tropical Medicine and Hygiene 91, no. 4 (2014): 699. DOI: 10.4269%2Fajtmh.14-0144. Copyright 2014 The American Society of Tropical Medicine and Hygiene. Posted with permission

First Report of Hepatitis E Virus Circulation in Domestic Pigs in Nigeria

Hepatitis E virus (HEV) is an important cause of acute hepatitis in humans. Zoonotic transmission between pigs and humans has been confirmed. Human HEV infection is common in Nigeria; however, characterization of HEV infection in other species was lacking. The objective of this study was to investigate HEV infection in Nigerian pigs. A total of 286 serum samples from six states in Nigeria were tested for presence of anti-HEV IgG. Ninety fecal samples from one of these states (Plateau State) were tested for presence of HEV RNA. The overall prevalence of anti-HEV IgG-positive or suspect-positive pigs was 55.6% (159 of 286) with regional prevalence rates ranging from 36% (9 of 25; Delta State) to 88% (22 of 25; Taraba State). The overall HEV RNA prevalence rate was 76.7% (69 of 90). All polymerase chain reaction-positive samples belonged to HEV genotype 3 based on sequencing. The results indicate that HEV genotype 3 infection is widespread in Nigerian pigs

Molecular characterisation of African swine fever viruses from Nigeria (2003–2006) recovers multiple virus variants and reaffirms CVR epidemiological utility

Samples collected from wild and domestic suids in Nigeria, over a 3-year period (2003–2006), were evaluated for African swine fever (ASF) virus genome presence by targeting three discrete genome regions, namely the 478-bp C-terminal p72 gene region advocated for genotype assignment, a 780-bp region spanning the 50-ends of the pB125R and pB646L (p72) genes and the hypervariable central variable region (CVR) encoded within the 9RL ORF (pB602L). ASF virus (ASFV) presence was confirmed in 23 of the 26 wild and domestic pigs evaluated. No evidence of ASF infection was found in two warthogs from Adamawa State; however, one bushpig from Plateau State was positive. Nucleotide sequences of the 478-bp and 780-bp amplicons were identical across all ASFV-positive samples sequenced. However, five discrete CVR variants were recovered, bringing the total number identified to date, from Nigeria, to six. The largest of the CVR variants, termed ‘Tet-36’ was identical to a virus causing outbreaks in neighbouring Benin in 1997, indicating a prolonged persistence of this virus type in Nigeria. Co-circulation of three tetramer types (Tet-36, Tet-27 and Tet-20) was found in Plateau State in July 2004, whilst in Benue State, two tetramer types (Tet-20 and Tet-21) were present in August 2005. Despite simultaneous field presence, individual co-infection was not observed. This study has reaffirmed the epidemiological utility of the CVR genome region for distinguishing between geographically and temporally constrained genotype I viruses, and has revealed the presence of multiple ASFV variants in Nigeria

Development of a fluorescent microbead-based immunoassay for the detection of hepatitis E virus IgG antibodies in pigs and comparison to an enzyme-linked immunoassay

Swine hepatitis E virus (HEV) is a zoonotic virus and pigs are considered as an important reservoir. Swine HEV infection is widespread and most pig herds are infected. Humans can be infected with swine HEV via consumption of undercooked pork or through direct contact with infected pigs. To minimize the risk of zoonotic transmission, sensitive tools to assess the HEV infection status of pigs and pork products are needed. The objective of this study was to develop a fluorescent microbead-based immunoassay (FMIA) for the detection of IgG antibodies against swine HEV and compare it to an in-house enzyme-linked immunoassay (ELISA). Three sets of samples were utilized: (A) samples from pigs infected experimentally with different strains of HEV (positive controls, n= 72), (B) samples from known HEV-negative pigs (negative controls, n= 62) and (C) samples from pigs of unknown HEV infection status (n= 182). All samples were tested by both ELISA and FMIA. The results on the experimental samples with known HEV exposure indicate that both assays have a specificity of 100% while the sensitivity ranges from 84.6% (ELISA) to 92.3% (FMIA). The overall prevalence of HEV IgG antibodies in field samples from pigs with unknown HEV exposure was 21.9% (40/182) for the ELISA and 21.4% (39/182) for the FMIA. The two assays had an almost perfect overall agreement (Kappa= 0.92)

Seroprevalence of Influenza A Virus in Dromedaries in North-Western Nigeria

Although influenza A virus is endemic in wild waterfowl, domestic poultry, swine, humans, bats, cetaceans, dogs, and horses, there is a paucity of data on the potential role of camels in zoonotic transmission of the virus. To estimate the seroprevalence of the influenza A virus in camel populations, four local government areas of Nigeria that share an international border with the Niger Republic were selected. Blood samples from 184 one-hump camels (dromedaries) were collected and tested for influenza IgG antigen by ELISA. Each camel’s demographic variable, such as age, gender, location, production system, and usage, was recorded. The overall seroprevalence rate of influenza virus IgG in this study was 10.33% (95%CI: 6.33–15.66%). In the bivariate model, there was no significant difference in gender, age, site location and production system, except for usage. There was a significantly lower seroprevalence rate among camels used for labour (odds ratio (OR) = 0.34, 95% CI: 0.10–0.97) than those used for meat consumption; however, not after adjusting for other variables in the model. Increase surveillance through early detection, prediction, and risk assessment of pathogens in animal reservoirs and environmental contamination as One Health strategies to reduce potential human spillover is recommended. Molecular epidemiology studies could better elucidate the role of camels in the dynamics of disease transmission pathways

Seroprevalence of Equine Influenza and Its Associated Risk Factors in Northwest Nigeria.

Equine influenza (EI) is a fast-spreading respiratory disease of equids caused by equine influenza A virus (EIV), often resulting in high morbidity and a huge economic impact on the equine industry globally. In this cross-sectional study to determine the seroprevalence of EI and its associated risk factors, sera from 830 horses bled on a single occasion in Northwest Nigeria between October 2019 and January 2020 were screened for antibodies to A/equine/Richmond/1/2007 (H3N8) using the single radial haemolysis (SRH) assay. Antibodies were detected in 71.3% (592/830, 95% CI: 68−74%) of horses (SRH area ≥ 0.5 mm2). Although there were statistically significant univariable associations between seropositivity and age, sex, breed, purpose and coat colour, only age remained significant when included with each of the other variables in bivariable analyses. There was a clear trend for increasing odds of seropositivity with increasing age: OR 1.6, 95% CI: 1.05−2.40 (p = 0.03) for 5−14-year-olds and OR 8.13, 95% CI: 2.75−24.1 (p 150 mm2, considered sufficient to protect against clinical disease and virus shedding. Comparative screening of a subset of the horses (n = 118) with a 2019 H3N8 virus (A/equine/Worcestershire/2019) revealed a significantly greater seropositivity (p = 0.0001) than A/equine/Richmond/1/2007 consistent with exposure of the population during a widespread outbreak of EI in the region in 2019. In conclusion, there was an insufficient level of protection against EI in the region and introduction of a vaccination programme with vaccines containing recently circulating virus is recommended to mitigate against further outbreaks of EI in Nigeria

Serological detection of antibodies against Hepatitis E virus among Camels (Camelus dromedarius) in Nigeria

Hepatitis E virus (HEV) is a zoonotic disease with increasing endemicity in many countries around the world. At the moment no data on the status of and epidemiology of HEV in camels an important livestock in the semi-arid and arid parts of Nigeria. This study determined HEV seroprevalence in two areas (with high population of camel in Nigeria) using indirect Enzyme Linked Immunosorbent Assay. Out of 88 camels sampled, HEV antibodies were detected in 27 suggesting a prevalence rate of 30.7%. Higher rates were observed among camels in Maigatari LGA (22.7%) compared with 7.9% in Suletankarkar LGA, Jigawa State. These findings reinforce the need for further studies on molecular characterization and evolutionary diversity in Camel as well as pastoralists in Nigeria

First Report of Hepatitis E Virus Circulation in Domestic Pigs in Nigeria

Hepatitis E virus (HEV) is an important cause of acute hepatitis in humans. Zoonotic transmission between pigs and humans has been confirmed. Human HEV infection is common in Nigeria; however, characterization of HEV infection in other species was lacking. The objective of this study was to investigate HEV infection in Nigerian pigs. A total of 286 serum samples from six states in Nigeria were tested for presence of anti-HEV IgG. Ninety fecal samples from one of these states (Plateau State) were tested for presence of HEV RNA. The overall prevalence of anti-HEV IgG-positive or suspect-positive pigs was 55.6% (159 of 286) with regional prevalence rates ranging from 36% (9 of 25; Delta State) to 88% (22 of 25; Taraba State). The overall HEV RNA prevalence rate was 76.7% (69 of 90). All polymerase chain reaction-positive samples belonged to HEV genotype 3 based on sequencing. The results indicate that HEV genotype 3 infection is widespread in Nigerian pigs