ANTI-INFLAMMATORY AND ANALGESIC EFFECTS OF AQUEOUS LEAF EXTRACTS OF Gomphrena celosioides AND Momordica charantia

This   study  reports the   anti-inflammatory   and  analgesic  properties  of   aqueous leaf   extracts  of  Gom-        phrena celosioides and Momordica charantia in rats and mice.   Hot plate latency test and acetic acid        induced writhing movement method were used as the model for analgesic evaluation, while the carra-        geenan-induced rat paw oedema was used as the model for anti-inflammatory activity.    The result of        the study revealed that the leaf extracts of the two plants possess anti-inflammatory property as they        were found to significantly (p<0.05) inhibit oedema induced by carrageenan in the rat paws. The leaf        extract of Momordica charantia was also found to significantly (p<0.05) increase the reaction time of        the mice in hot plate test method, while the number of writhing movement of the mice was also signifi-        cantly(p<0.05) reduced in dose-dependent manner. Similar result was observed for mice treated with        extract   of  Gomphrena   celosioides. The  pilot toxicity   study   revealed   the   plants  to   have  appreciable        safety margin, but high dose of Momordica charantia could cause purgation in rats. The results of the        study   suggest   the   anti-inflammatory   and   analgesic   effects   of   the   aqueous   leaf   extracts   of   the   two        plants.&nbsp

Factors Affecting Perceived Stigma in Leprosy Affected Persons in Western Nepal

Background There are various factors which construct the perception of stigma in both leprosy affected persons and unaffected persons. The main purpose of this study was to determine the level of perceived stigma and the risk factors contributing to it among leprosy affected person attending the Green Pastures Hospital, Pokhara municipality of western Nepal. Methods A cross-sectional study was conducted among 135 people affected by leprosy at Green Pastures Hospital and Rehabilitation Centre. Persons above the age of 18 were interviewed using a set of questionnaire form and Explanatory Model Interview Catalogue (EMIC). In addition, two sets of focused group discussions each containing 10 participants from the ward were conducted with the objectives of answering the frequently affected EMIC items. Results Among 135 leprosy affected persons, the median score of perceived stigma was 10 while it ranged from 0–34. Higher perceived stigma score was found in illiterate persons (p = 0.008), participants whose incomes were self-described as inadequate (p = 0.014) and who had changed their occupation due to leprosy (p = 0.018). Patients who lacked information on leprosy (p = 0.025), knowledge about the causes (p = 0.02) and transmission of leprosy (p = 0.046) and those who had perception that leprosy is a severe disease (p<0.001) and is difficult to treat (p<0.001) had higher perceived stigma score. Participants with disfigurement or deformities (p = 0.014), ulcers (p = 0.022) and odorous ulcers (p = 0.043) had higher perceived stigma score. Conclusion The factors associated with higher stigma were illiteracy, perceived economical inadequacy, change of occupation due to leprosy, lack of knowledge about leprosy, perception of leprosy as a severe disease and difficult to treat. Similarly, visible deformities and ulcers were associated with higher stigma. There is an urgent need of stigma reduction strategies focused on health education and health awareness programs in addition to the necessary rehabilitation support

HEMAGLUTINAÇÃO DOS ERITRÓCITOS DE MAMÍFEROS POR CEPAS DE VÍRUS DA DOENÇA DE NEWCASTLE ISOLADOS NA NIGÉRIA CENTRAL

The haemagglutinability of mammalian erythrocytes by field and vaccine strains of theNewcastle disease virus was assessed. Variability in the pattern of agglutination of thevarious specie erythrocytes was observed. Whereas noticeable differences in the patternof agglutination between field virus and vaccine strains were not apparent, differencesbetween the velogenic and non-velogenic strains was observed. The possibility ofdeveloping haemagglutinability tests using mammalian erythrocytes for rapid straindifferentiation in less developed laboratories is discussed.A hemaglutinação dos eritrócitos de mamíferos por cepas de campo e vacinais do vírusda doença de Newcastle foi avaliada. Foi verificado variabilidade no modelo deaglutinação entre as várias espécies. Embora diferenças perceptíveis no padrão deaglutinação entre vírus de campo e cepas vacinais não tenham sido evidentes, diferençasentre as cepas velogénicas e não velogénicas foram observadas. A possibilidade dedesenvolvimento de testes hemaglutinação utilizando eritrócitos de mamíferos para arápida diferenciação de cepas em laboratórios menos desenvolvidos é aqui discutida

Farmers’ perception and Knowledge need for adoption of new cultivars of cassava in Igabi Local Government Area (LGA), Kaduna State.

The study examined farmers’ perception and knowledge need for adoption new varieties of cassava in Igabi LGA, Kaduna State. The purpose of the research was to;( 1) ascertain how cassava farmers in the area perceive the new varieties of cassava vis-a-vis the technologies required for its propagation, and (2) the relationship between the existing and recommended knowledge of cassava cultivation in the area. Seventy six respondents used for the study were selected by random sampling while focus group discussion and interview schedule were employed to gather data. Data were analyzed by mean score analysis. The result of the analysis shows that majority (36.8%) were between 30- 39 years, 89% married and 34.2% had secondary education while 50% had regular extension contact. However, out of five (5) main recommended production technologies, only two- planting methods (51.3%) and weeding interval (56.6%) were in line with farmers’ previous experience or knowledge. Farmers perceived the new cassava cultivars as beneficial through its high yielding capacity (3.3*), fast maturity (3.1) and disease resistance (2.5) among others, while capital and marketing (3.2 apiece), farm size (3.0) were among the perceived constraints to adoption of the new cultivars. The study recommended among others formation of cooperative society to aid farming activities in the area. Keywords: awareness, benefit, constraints, knowledge of planting cassava, perception

SELECTED WELFARE PARAMETERS OF BROILER CHICKENS ON DIFFERENT FEED QUANTITY AND TIME

This study was conducted to determine welfare of broiler chickens fed ad libitum and restricted feed during early (08.00hour) and late (16:00hour) of the day. One hundred and eighty (21d old) broiler chickens were randomly allocated to 4 treatments in a 2x2 factorial arrangement comprising of ad libitum and restricted feeding (75% of ad libitum) quantity at 8 and 16 hours feeding time in three replicates. Data were collected on leg problem, dust bathing, body temperature, mortality and haematology. Result revealed that neither feed quantity nor time had significant (P&gt;0.05) effect on leg problem, dust bathing and mortality of broiler chickens. Broiler chickens under ad lib feed quantity and 16:00hours feeding time had (P&lt;0.05) highest body temperature (40.93oC) while those under ad libitum morning (08:00hours) recorded the least (40.75°C). Pack Cell Volume had similar (P&gt;0.05) increase from 26.75% at the beginning (21d) to 31.25 (%) at 56d with broiler chickens under restricted feeding and fed at 16:00hours feeding time. Basophil count reduced from a range of 0.5-2% (P&lt;0.05) to 1% (P&gt;0.05) with restricted feeding. Also eosinophil reduced from 2-4% (P&lt;0.05) range to 0-2.5% (P&gt;0.05) with restricted feeding while birds under ad lib increased from 0-1% (P&lt;0.05) at 21d to 2.75% (P&gt;0.05) at 56d. Better welfare (good body temperature) of broiler chicken could be achieved with early feeding time irrespective of quantity. However, restricted feeding could be explored in broiler production where reduced basophil, eosinophil and stress are vital for enhanced welfare and performance. &nbsp; &nbsp

Environmental Susceptibility and Resistance to Coronavirus Disease-19 (COVID-19): A Review

It is believed that certain environmental factors modulate coronavirus disease-19 (COVID-19). This review outlines the role of environmental factors in COVID-19 infectivity, spread, and severity. Relevant articles were retrieved from Google Scholar, SpringerLink, and Scopus, then pooled and duplicates removed with EndNote software. Available information reveals that temperature, relative humidity (RH), sunlight, pollutants, and population density modulate COVID-19 infectivity and pathogenicity. COVID-19 spread is promoted by low temperature (&lt; 25 °C) and RH (&lt; 40%), whereas it is inhibited by high temperature (&gt; 25 °C) and RH (&gt; 40%). Sunlight exposure alters the virus’s genetic material and boosts the host’s immune function by raising serum vitamin D (25-hydroxyvitanim D), reducing the virus’s viability and replication. Prolonged indoor stays with poor ventilation cause re-breathing of the air and increase carbon dioxide concentration, particularly in crowded rooms, predisposing to COVID-19. Pollutants, including particulate matter, nitrogen dioxide, ozone, and sulphur dioxide, may overexpress the virus’s receptor called angiotensin-converting enzyme 2 (ACE2), thereby increasing the virus’s infectivity. Pollutants may also induce inflammation of the respiratory tract, weakening the immune function and thereby increasing susceptibility to COVID-19. High population density increases body contact and thus susceptibility to the virus. To stem the incidence and mortality of COVID-19, the mentioned environmental factors must be kept at healthy levels

Surveillance for Avian Influenza H 5 Antibodies and Viruses in Commercial Chicken Farms in Kano State, Nigeria

Abstract: Outbreaks of highly pathogenic Avian Influenza occurred previously for 3 consecutive years, 2006, 2007 and 2008, in Kano State, Nigeria, causing heavy economic losses to farmers and the government. It was against this background that Avian Influenza (AI) surveillance study in commercial poultry farms in the State was conducted. Haemagglutination Inhibition (HI) test was conducted to determine the presence of AI H 5 antibodies in 1,160 sera obtained from flocks in 33 Avian influenza affected (AF) and 25 Non Avian influenza-affected (NAF) farms. To complement the study, 320 cloacal swabs obtained from flocks in farms that were serologically positive for AI H 5 antibodies, were further subjected to Reverse Transcription-Polymerase Chain Reaction (RT-PCR), to determine if the chickens were shedding AI viruses. Of the 1,160 sera tested, 150 (12.9%) were positive for AI H 5 antibodies, with flocks in 16 (27.6%) of the farms being positive. Prevalence rates of 14.1 and 11.4% and mean antibody titres of 5.4±0.2 and 4.6±0.1 log 2 for AI H 5 antibodies were obtained for AF and NAF farms, respectively. The RT-PCR results showed that all the 320 cloacal swabs tested were negative for AI H 5 viruses. The antibodies detected between flocks in the AF and NAF farms might be attributed to vaccination and the titres determined were above the minimum protection level recommended by the OIE. It was recommended that vaccination of chickens against AI should be discouraged because it may interfere with the stamping out policy adopted by Nigeria in the control and eradication of the disease

Paediatric brought-in-dead at a tertiary health facility in South western Nigeria: Patterns and drivers

Background: ‘Brought- in-dead’ (BID) refers to the demise of an individual before presentation to a health facility. This study assessed the pattern of paediatric BID cases seen at a tertiary health facility in southwest Nigeria. Method: A cross-sectional, descriptive study was done at the Children Emergency Ward (CEW) of the hospital between January 2014 and December 2018. The patterns of BID cases and presumed causes of death were determined using a standardized checklist adapted from the WHO verbal autopsy instrument. Results: Ninety-eight BID cases were seen during the study, constituting 2.5% of total patients seen during the period. The median (IQR) age of cases was 24.0 (8.75 – 63.0) months and 72.4% were under-fives. Most had symptoms related to the haematologic (36.7%), respiratory (24.5%) or digestive (20.4%) systems. Severe anaemia 31(31.6%), gastroenteritis 19 (19.4) and aspiration 17 (17.3%) were the most common causes of death. The median (IQR) duration of illness before presentation was 3.0 (1.0 – 7.0) days but most presented from 4 – 7 days of illness. A significant relationship was found between the duration of illness and whether or not pre-hospital treatment was received (p &lt; 0.0001). Unprescribed drugs purchased over the counter were the most commonly used treatment in 79.1% of cases (p &lt; 0.0001). Conclusion: This study has highlighted the prevalence and pattern of paediatric BID in a tertiary health facility in southwest Nigeria and the factors that were associated with it. More efforts need to be geared towards community sensitization and pediatric health care to prevent factors drivingits menace

Musculoskeletal symptoms and non-prescribed treatments are common in an urban African population of people living with HIV.

There are no data from West Africa reporting musculoskeletal (MSK) disease in people living with HIV (PLWH). Our primary outcome was to measure the prevalence of MSK symptoms in PLWH in urban West Africa. Our secondary outcomes were to describe the disability, impact on work and treatment use associated with the presence of MSK pain. We conducted an e-questionnaire-based point prevalence study of musculoskeletal symptoms, associated disability and treatment in 292 PLWH attending routine follow-up in Lagos, Nigeria. Seventy-three (25%) patients reported MSK pain; 28 (38%) reported chronic symptoms (> 3 months). HIV suppression rates were high in this population (n = 240, 82%) and comparable between individuals with and without chronic pain. MSK pain was associated with female gender and higher body mass index (BMI). Mechanical pain was the most common pain syndrome identified (n = 34, 47%). Lumbar spine and knee were the most common sites. Chronic pain was associated with increased disability compared with the presence of any MSK pain. High rates of treatment-seeking behaviour were seen in those individuals reporting MSK pain (n = 62, 85%). The majority of these individuals sought traditional treatments (n = 48, 66%). Chronic MSK pain and non-prescribed treatments are common in PLWH established on ART in urban West Africa. Studies are required to measure the long-term impact of these symptoms and medicines on retention in HIV care and ART adherence, besides other long-term health outcomes