PECULIAIRITIES OF THE INFLUENCE NITROGEN MONOXIDE SYNTHESIS BLOCKADE ON CIRCADIAN ORGANIZATION OF KIDNEY ACID-REGULATING FUNCTION UNDER CONDITIONS OF PINEAL GLAND HYPERFUNCTION

Introduction: In recent decades chronobiology - a science that studies the regularities of the functioning of the organism, especially biological rhythms is developing rapidly. Biorhythms are stable nonstopper lifetime fluctuations with individual amplitude-frequency characteristics. Kidneys are characterized by a clear time organization of functions too, however, the peculiarities of circadian organization and the mechanisms of the participation of intracellular messengers in particular nitrogen monoxide (NО) in biorhythmic regulation of renal functions, remain insufficiently studied The aim of our work was to investigate the peculiairities of the influence nitrogen monoxide synthesis blockade on circadian organization of kidney acid-regulating function under conditions of pineal gland hyperfunction. Materials and methods: The experiments were conducted on 72 mature non-linear albino male rats with their body mass 0,15-0,18 kg. The animals were kept under vivarium conditions at a stable temperature and air humidity fed on a standard dietary intake. The control group included animals (n=36) kept under conditions of usual light regimen (12.00L:12.00D) during 7 days. The experimental group included animals (n=36) injected with N-nitro-L-arginine (L-NNA) in the dose of 20 mg/kg during 7 days under conditions of continuous absolute darkness (12.00D:12.00D). On the 8th day the animals were exposed to 5% water load with heated to room temperature water supplied and the parameters of the kidney acid-regulating function under conditions of forced diuresis were investigated. Results: The inhibition of the monoxide synthesis (NО) in conditions of pineal gland (PG) hyperfunction violated the structures of chronorhythms acid-regulating function of the kidneys. The decrease in urine pH at daytime intervals was due to a decrease in the level of hydrogen ion excretion during this period of the day. The introduction of the NО blocker against the background of the PG hyperfunction violated the structure of the acid esterification chronorhythms that are titrated. The maximum level of this indicator was recorded at 20.00 a.m. a day. The blockade of the synthesis NО on the background of the PG hyperfunction significantly reduced the level of excretion of ammonia. Conclusions: The maximum level of excretion was 12.00 a.m., bathyphase - at 24.00 a.m. The average daily level was 1.5 mcmol/100 mcl GF and was lower relative to the control animals and animals that were in the conditions of PG hyperfunction

Influence of plasmapheresis on anti-ovarian antibodies level before in vitro fertilization in women with infertility

Introduction. The study of anti-ovarian autoantibodies in patients with ovarian insufficiency against the background of normal levels of gonadotropins, prolactin and androgens shows that the incidence of autoimmune oophoritis among such women varies from 19.2% to 31.5%. The objective of the study was to study the anti-ovarian antibodies level in infertile women at the preparatory stage for in vitro fertilization. Materials and methods. We examined 30 women, who were divided into the following groups: control group – 10 reproductively healthy women, who had a birth in the history (from 1 to 3); study group – 20 women with infertility of tube origin, who were included in the in vitro fertilization program. The study was performed between 2015-2017 in the Bukovinian State Medical University and Centre of Reproductive Medicine, Ukraine. The women signed the informed consent. Results. The anti-ovarian antibodies level in infertile women was found out to be 1.7 times less than in the control group. Reduction of the level of anti-ovarian antibodies in patients from the study group was marked by the obstetric and gynecological history. Conclusions. Anti-ovarian antibodies are present in both reproductively healthy women and in patients with infertility. The level of anti-ovarian antibodies can be both increased and reduced, depending on the pathological process that caused infertility. In both cases, this fact can testify the infertility. After plasmapheresis, the increased level of anti-ovarian antibodies sharply decreases

Mueller-matrix imaging polarimetry elevated by wavelet decomposition and polarization-singular processing for analysis of specific cancerous tissue pathology

Significance Mueller-matrix polarimetry is a powerful method allowing for the visualization of malformations in biological tissues and quantitative evaluation of alterations associated with the progression of various diseases. This approach, in fact, is limited in observation of spatial localization and scale-selective changes in the poly-crystalline compound of tissue samples. Aim We aimed to improve the Mueller-matrix polarimetry approach by implementing the wavelet decomposition accompanied with the polarization-singular processing for express differential diagnosis of local changes in the poly-crystalline structure of tissue samples with various pathology. Approach Mueller-matrix maps obtained experimentally in transmitted mode are processed utilizing a combination of a topological singular polarization approach and scale-selective wavelet analysis for quantitative assessment of the adenoma and carcinoma histological sections of the prostate tissues. Results A relationship between the characteristic values of the Mueller-matrix elements and singular states of linear and circular polarization is established within the framework of the phase anisotropy phenomenological model in terms of linear birefringence. A robust method for expedited (up to ∼15 min) polarimetric-based differential diagnosis of local variations in the poly-crystalline structure of tissue samples containing various pathology abnormalities is introduced. Conclusions The benign and malignant states of the prostate tissue are identified and assessed quantitatively with a superior accuracy provided by the developed Mueller-matrix polarimetry approach

Mueller-matrix imaging polarimetry elevated by wavelet decomposition and polarization-singular processing for analysis of specific cancerous tissue pathology

Abstract Significance: Mueller-matrix polarimetry is a powerful method allowing for the visualization of malformations in biological tissues and quantitative evaluation of alterations associated with the progression of various diseases. This approach, in fact, is limited in observation of spatial localization and scale-selective changes in the poly-crystalline compound of tissue samples. Aim: We aimed to improve the Mueller-matrix polarimetry approach by implementing the wavelet decomposition accompanied with the polarization-singular processing for express differential diagnosis of local changes in the poly-crystalline structure of tissue samples with various pathology. Approach: Mueller-matrix maps obtained experimentally in transmitted mode are processed utilizing a combination of a topological singular polarization approach and scale-selective wavelet analysis for quantitative assessment of the adenoma and carcinoma histological sections of the prostate tissues. Results: A relationship between the characteristic values of the Mueller-matrix elements and singular states of linear and circular polarization is established within the framework of the phase anisotropy phenomenological model in terms of linear birefringence. A robust method for expedited (up to ∼15 min) polarimetric-based differential diagnosis of local variations in the poly-crystalline structure of tissue samples containing various pathology abnormalities is introduced. Conclusions: The benign and malignant states of the prostate tissue are identified and assessed quantitatively with a superior accuracy provided by the developed Mueller-matrix polarimetry approach