4 research outputs found
Insulin receptor substrate 1 gene expression is strongly up-regulated by HSPB8 silencing in U87 glioma cells
Objective. The aim of the present investigation was to study the expression of genes encoding
IRS1 (insulin receptor substrate 1) and some other functionally active proteins in U87 glioma cells
under silencing of polyfunctional chaperone HSPB8 for evaluation of the possible significance of
this protein in intergenic interactions.
Methods. Silencing of HSPB8 mRNA was introduced by HSPB8 specific siRNA. The expression
level of HSPB8, IRS1, HK2, GLO1, HOMER3, MYL9, NAMPT, PER2, PERP, GADD45A, and DEK
genes was studied in U87 glioma cells by quantitative polymerase chain reaction.
Results. It was shown that silencing of HSPB8 mRNA by specific to HSPB8 siRNA led to a
strong down-regulation of this mRNA and significant modification of the expression of IRS1 and
many other genes in glioma cells: strong up-regulated of HOMER3, GLO1, and PERP and downregulated of MYL9, NAMPT, PER2, GADD45A, and DEK gene expressions. At the same time, no
significant changes were detected in the expression of HK2 gene in glioma cells treated by siRNA,
specific to HSPB8. Moreover, the silencing of HSPB8 mRNA enhanced the glioma cells proliferation rate.
Conclusions. Results of this investigation demonstrated that silencing of HSPB8 mRNA affected the expression of IRS1 gene as well as many other genes encoding tumor growth related
proteins. It is possible that the dysregulation of most of the studied genes in glioma cells after
silencing of HSPB8 is reflected by a complex of intergenic interactions and that this polyfunctional
chaperone is an important factor for the stability of genome function and regulatory mechanisms
contributing to the tumorigenesis control
Insulin receptor substrate 1 gene expression is strongly up-regulated by HSPB8 silencing in U87 glioma cells
Objective. The aim of the present investigation was to study the expression of genes encoding IRS1 (insulin receptor substrate 1) and some other functionally active proteins in U87 glioma cells under silencing of polyfunctional chaperone HSPB8 for evaluation of the possible significance of this protein in intergenic interactions
Insulin receptor substrate 1 gene expression is strongly up-regulated by HSPB8 silencing in U87 glioma cells
Objective. The aim of the present investigation was to study the expression of genes encoding
IRS1 (insulin receptor substrate 1) and some other functionally active proteins in U87 glioma cells
under silencing of polyfunctional chaperone HSPB8 for evaluation of the possible significance of
this protein in intergenic interactions.
Methods. Silencing of HSPB8 mRNA was introduced by HSPB8 specific siRNA. The expression
level of HSPB8, IRS1, HK2, GLO1, HOMER3, MYL9, NAMPT, PER2, PERP, GADD45A, and DEK
genes was studied in U87 glioma cells by quantitative polymerase chain reaction.
Results. It was shown that silencing of HSPB8 mRNA by specific to HSPB8 siRNA led to a
strong down-regulation of this mRNA and significant modification of the expression of IRS1 and
many other genes in glioma cells: strong up-regulated of HOMER3, GLO1, and PERP and downregulated of MYL9, NAMPT, PER2, GADD45A, and DEK gene expressions. At the same time, no
significant changes were detected in the expression of HK2 gene in glioma cells treated by siRNA,
specific to HSPB8. Moreover, the silencing of HSPB8 mRNA enhanced the glioma cells proliferation rate.
Conclusions. Results of this investigation demonstrated that silencing of HSPB8 mRNA affected the expression of IRS1 gene as well as many other genes encoding tumor growth related
proteins. It is possible that the dysregulation of most of the studied genes in glioma cells after
silencing of HSPB8 is reflected by a complex of intergenic interactions and that this polyfunctional
chaperone is an important factor for the stability of genome function and regulatory mechanisms
contributing to the tumorigenesis control