Creative industries as an innovative way to sustainable development

The creative industries refer to a range of economic activities which are concerned with the generation or exploitation of knowledge and information. They may variously also be referred to as the cultural industries (especially in Europe) or the creative economy. The creative industries have been seen to become increasingly important to economic well-being, proponents suggesting that “human creativity is the ultimate economic resource” and that “the industries of the twenty-first century will depend increasingly on the generation of knowledge through creativity and innovation and will lead to the sustainable development”. When you are citing the document, use the following link http://essuir.sumdu.edu.ua/handle/123456789/3114

Язык, речь, личность в зеркале психолингвистики

В издании раскрываются вопросы психологии языка, речи и личности. Исследуются проблемы переводческих универсалий. Особое внимание уделяется сравнительно новым научным понятиям и категориям: языковая личность, языковая картина мира, чувство языка, речевой опыт. Для научных работников, аспирантов, студентов и всех, кто интересуется вопросами психологических особенностей речевой деятельности.СОДЕРЖАНИЕ Предисловие 4; Часть 1. Язык и мир. Речь и личность 6; Засекина Л. В. Интеллект личности в зеркале психолингвистики 6; Засекин С. В. Язык в зеркале перевода: психолингвистическое исследование переводческих универсалий 25; Лавриненко А. Л. Когнитивно-мотивационные особенности языковой личности 53; Орап М. О. Психология речевого опыта личности 73; Савченко Е. В. Нарративный подход в исследовании индивидуального опыта на личностном уровне 94; Фомина Н. А. Соотношение различных свойств языковой личности и особенностей ее речевой деятельности 126; Часть 2. Психология языка, речи и речевой деятельности личности 148; Белык Т. Н. Психологические особености речевого имиджа личности 148; Дячук Н. В. Творческий потенциал будущего переводчика художественных текстов в его профессиональной деятельности 162; Крупыч С. О. Психологические проявления личности в речевой деятельности 176; Тарасюк И. В. Психологические основы речевой адаптации детей мигрантов 198; Шишкина К. Ю. Информационная безопасность личности в контексте психолингвистики 216; Часть 3. Психолингвистические исследования речи личности: норма-аномалия-патология 223; Василюк О. П. Психолингвистические средства реорганизации травматической памяти личности 223; Соловей О. А. Речевая деятельность детей с синдромом дефицита внимания и гиперактивности 244; Тригуб Г. В. Психологические основы овладения иностранным языком младшими школьниками с ведущей левой рукой 268; Об авторах 288

<i>Spidey</i> expression during development is essential for adult survival.

<p>(<b>A</b>) Silencing <i>spidey</i> expression from 0–72 hours after egg laying (AEL) results in pharate lethality of males and females. Male survivorship significantly improves when <i>spidey</i> is suppressed from 96 hours AEL but few females successfully eclose. Survival % is calculated as the number of successfully eclosed adults relative to the total number of male and female embryos; E: embryo; L1: 1^st instar larvae; L2: 2^nd instar larvae; L3: 3^rd instar larvae; P: pupae. N = 50 embryos for each stage. See <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1006126#pgen.1006126.s012" target="_blank">S6 Table</a> for full genotypes. (<b>B</b>) Top: <i>oeno>spidey</i>^<i>RNAi</i> flies fail to emerge from the pupal case. Bottom left: malformed legs (arrowheads) are shorter in <i>oeno>spidey</i>^<i>RNAi</i> males and females compared to genetic controls (bottom right). (<b>C</b>) Representative DART MS spectra from <i>oeno>spidey</i>^<i>RNAi</i> males reveals an overall decrease of major cuticular lipid signals (blue, arrows) relative to tricosene (peak 1, red). In spectra from control lines, pentacosene (peak 4) and tricosene (peak 1) exhibit similar relative intensity levels. (<b>D</b>) Continuous overexpression of <i>spidey</i> at 29°C during development results in significant pre-pupal and pharate lethality compared to controls raised at 19°C (p<0.0001 for <i>oeno>spidey</i> and <i>oeno>spidey</i>.<i>HA</i>, compared to controls, Chi-square test, n = 189). (<b>E</b>) Top: <i>oeno>spidey</i> and <i>oeno>spidey</i>.<i>HA</i> overexpression lines exhibit pre-pupal lethality and defects in head (arrowheads) and spiracle (*) eversion. Bottom: controls raised at 19°C. (<b>F</b>) Knockdown or overexpression of <i>spidey</i> at 29°C alters <i>Cyp18a1</i> and <i>spidey</i> transcript levels relative to control levels measured at 19°C. <i>Rp49</i> was used as an internal control for normalization. Data represent the average of 3 experimental replicates ± SEM.</p

Partial rescue of <i>spidey</i> defects by 20HE.

<p>(<b>A</b>) At 8 days old, 20HE supplementation restores total CHC levels to near control conditions in male but not female <i>spidey</i>^<i>KD</i> flies. Data shown are the mean ± SEM from 3 experimental replicates with 8 flies each; one-way ANOVA with Tukey’s multiple comparison test, *p<0.05, ns: not significant; a.u.: arbitrary units. See <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1006126#pgen.1006126.s012" target="_blank">S6 Table</a> for full genotypes. (<b>B</b>) At 20 days old, GFP signal intensity of oenocytes in <i>oeno>spidey</i>^<i>RNAi</i> males remains low and aberrantly distributed despite 20HE supplementation. (<b>C</b>) Females exhibit a significant increase in GFP intensity with 20HE feeding. Mean GFP intensity ± SEM shown; samples sizes are indicated above; Student’s t-test, **: p<0.01; ns: not significant.</p

<i>Spidey</i> is essential for cuticular lipid synthesis in adult flies.

<p>(<b>A</b>) Two different feeding regimes of adult flies are used to produce an immediate or delayed knockdown of <i>spidey</i> expression. Gene suppression is achieved by feeding RU-486 to <i>GSoeno>spidey</i>^<i>RNAi</i> flies; suppression is removed by placing flies on standard food. Cuticular lipids are extracted at 8 days old. A 65–85% decrease in CHC levels is apparent when <i>spidey</i> is suppressed using the immediate knockdown regime but not the delayed regime. Data represent the mean intensity of 3 experimental replicates (8 flies each) ± SEM; Student’s t-test, *p<0.05; a.u.: arbitrary units. See <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1006126#pgen.1006126.s012" target="_blank">S6 Table</a> for full genotypes. (<b>B</b>) Immediate and delayed knockdown of <i>spidey</i> over 15 days. The CHC levels do not recover at 15 days when <i>spidey</i> is suppressed from age 0–2 days. <i>Spidey</i> suppression between age 10–12 days produces an intermediate effect on CHC levels. Data shown represent the mean ± SEM from 3 experimental replicates with 8 flies each); Student’s t-test, *p<0.05. (<b>C</b>) Representative GCMS chromatograms of cuticular lipid extract from 15 day old <i>spidey</i>^{<i>control</i>} and <i>spidey</i>^<i>KD</i> males. Major peaks are labelled with the number of carbon atoms followed by number of double bonds; T: tricosene; P: pentacosene; STD: C26:0 standard spiked into the hexane solvent.</p

Quantification of ecdysteroids and ecdysonic acids metabolites by LC-MS/MS.

<p>(<b>A</b>) Top row: 20HE-oic acid levels were significantly elevated in <i>spidey</i>^<i>KD</i> L3 larvae, resulting in a lower 20HE to 20HE-oic acid ratio. 20HE-oic acid was not detected in L1 and L2 animals. Bottom row: makisterone A (MA) and makisteronoic acid (MA-oic acid) levels were lower in <i>spidey</i>^<i>KD</i> animals compared to <i>spidey</i>^{<i>control</i>} at mid-pupal stage (P). However, the ratio of MA to MA-oic acid in pupal stage was not significantly different. (<b>B</b>) Following overexpression of <i>spidey</i>, L3 larvae exhibit a significant decrease of 20HE and MA compared to controls. Data represent the average log-transformed values of 3 experimental replicates ± SEM; Student’s t-test.</p

Transgenic lines identified by RNAi/ MS screen.

<p>Transgenic lines identified by RNAi/ MS screen.</p

Schematic of RNA interference screen used to identify genes expressed in the oenocytes that contribute to cuticular lipid production.

<p>(<b>A</b>) Tissue-specific knockdown of the expression of 80 candidate genes was performed using 2 different oenocyte-expressing drivers, <i>oeno-Gal4</i> and <i>dsx-Gal4</i>. (<b>B)</b> Progeny from each cross were assayed individually in the DART MS source (arrowhead). (<b>C</b>) Chemical profiles obtained from female and male cuticles. The intensity index for molecules of interest (in blue) is calculated by normalizing signal intensity to an internal reference peak (in red: 7,11-HD for females, 7-T for males); 7-T: (<i>Z</i>)-7-tricosene; 7-P: (<i>Z</i>)-7-pentacosene; 7,11-PD: (<i>Z</i>, <i>Z</i>)-7,11-pentacosadiene; 7,11-HD: (<i>Z</i>, <i>Z</i>)-7,11-heptacosadiene; 7,11-ND: (<i>Z</i>, <i>Z</i>)-7,11-nonacosadiene. (<b>D</b>) Transgenic lines displaying intensity indices which are 2 standard deviations (SD) above or below the average value are subjected to a secondary DART MS screen and quantification by GCMS. (<b>E</b>) Chemical structures of the major CHC species characteristic of male or female cuticular profiles.</p