A review of challenges to genetic improvement of indigenous livestock for improved food production in Nigeria

Indigenous livestock production is intertwined with the life style of Nigerians. The Nigerian livestock industry employs the bulk of the rural work-force, and indigenous livestock breeds are abundant in the industry. These breeds are distributed across the diverse agro-ecological production systems, and are carriers of unique and responsive genotypes shaped by the needs of their managers. Despite their unique features, most indigenous livestock breeds are characteristically low in production and productivity. Improvement of these breeds represents a logical starting point for improving food security and agricultural productivity in Nigeria. To better understand natural genetic variation in these indigenous livestock breeds and strategies for improvement, better genetic characterization is required. Molecular assisted selection (MAS) will be valuable in the pursuit of selection for increased production, but the application of MAS to livestock improvement is constrained by a variety of limitations. The focus of this paper is to elucidate the potential of MAS as a tool for genetic improvement of indigenous livestock, to identify constraints and challenges in MAS implementation and propose solutions to increasing MAS feasibility in pursuit of improved food security and sustainability in Nigeria.Keywords: Food security, Genetic improvement, Genomic Selection, Indigenous livestock, Marker assisted selection, Nigeri

CHARACTERISATION OF MULTIVARIATE TRAITS IN SWAZI INDIGENOUS GOATS

Swazi goats from two different regions in Swaziland kept in communal systems were characterised based on phenotypic measurements. Objective measurements for 200 goats included body weight (BW), abdominal circumference (AC), body length (BL), chest circumference (CC), face length (FL), neck circumference (NC), neck length (NL), rump height (RH) and height at withers (HW), while qualitative traits included coat colour, coat texture and presence or absence of horns. Seven coat colours were observed across both regions. Goats with straight coat texture (82%), short hair length (68%) and with horns (84%) were predominantly more. Goats in Manzini region had the lower BW, AC, BL and CC (51.77±1.32, 61.77±1.32, 62.49±0.66, 57.96±1.18) than Shiselweni goats (53.09±1.01, 71.09±1.00, 73.67±0.94, 53.83±0.90). The study provided a reference for phenotypic and genetic diversity for Swazi goats useful for designing genetic strategies for improved production, food security and sustainable utilisation

The origin of domestication genes in goats

Goat domestication was critical for agriculture and civilization, but its underlying genetic changes and selection regimes remain unclear. Here, we analyze the genomes of worldwide domestic goats, wild caprid species, and historical remains, providing evidence of an ancient introgression event from a West Caucasian tur-like species to the ancestor of domestic goats. One introgressed locus with a strong signature of selection harbors the MUC6 gene, which encodes a gastrointestinally secreted mucin. Experiments revealed that the nearly fixed introgressed haplotype confers enhanced immune resistance to gastrointestinal pathogens. Another locus with a strong signal of selection may be related to behavior. The selected alleles at these two loci emerged in domestic goats at least 7200 and 8100 years ago, respectively, and increased to high frequencies concurrent with the expansion of the ubiquitous modern mitochondrial haplogroup A. Tracking these archaeologically

A Mapping Literature Review of Medical Cannabis Clinical Outcomes and Quality of Evidence in Approved Conditions in the USA from 2016 to 2019

In 2017, a National Academies of Sciences, Engineering, and Medicine (NASEM) report comprehensively evaluated the body of evidence regarding cannabis health effects through the year 2016. The objectives of this study are to identify and map the most recently (2016-2019) published literature across approved conditions for medical cannabis and to evaluate the quality of identified recent systematic reviews, published following the NASEM report. Following the literature search from 5 databases and consultation with experts, 11 conditions were identified for evidence compilation and evaluation: amyotrophic lateral sclerosis, autism, cancer, chronic noncancer pain, Crohn's disease, epilepsy, glaucoma, human immunodeficiency virus/AIDS, multiple sclerosis (MS), Parkinson's disease, and posttraumatic stress disorder. A total of 198 studies were included after screening for condition-specific relevance and after imposing the following exclusion criteria: preclinical focus, non-English language, abstracts only, editorials/commentary, case studies/series, and non-U.S. study setting. Data extracted from studies included: study design type, outcome definition, intervention definition, sample size, study setting, and reported effect size. Few completed randomized controlled trials (RCTs) were identified. Studies classified as systematic reviews were graded using the Assessing the Methodological Quality of Systematic Reviews-2 tool to evaluate the quality of evidence. Few high-quality systematic reviews were available for most conditions, with the exceptions of MS (9 of 9 graded moderate/high quality; evidence for 2/9 indicating cannabis improved outcomes; evidence for 7/9 indicating cannabis inconclusive), epilepsy (3 of 4 graded moderate/high quality; 3 indicating cannabis improved outcomes; 1 indicating cannabis inconclusive), and chronic noncancer pain (12 of 13 graded moderate/high quality; evidence for 7/13 indicating cannabis improved outcomes; evidence from 6/7 indicating cannabis inconclusive). Among RCTs, we identified few studies of substantial rigor and quality to contribute to the evidence base. However, there are some conditions for which significant evidence suggests that select dosage forms and routes of administration likely have favorable risk-benefit ratios (i.e., epilepsy and chronic noncancer pain). The body of evidence for medical cannabis requires more rigorous evaluation before consideration as a treatment option for many conditions, and evidence necessary to inform policy and treatment guidelines is currently insufficient for many conditions

Morphologic variations of periwinkle and profiles of periwinkle marketers and harvesters in two states in the Niger delta area of Nigeria

Periwinkles are invertebrates rich in protein and omega-3 fatty acids. These make them relished delicacies. Three hundred and fifteen (315) individual periwinkles were hand-picked from mangrove swamp river banks and intertidal zones of Twon-Brass in Bayelsa State and Abonnema town of Rivers State. Shell length and the weight of both the meat and shell were taken with Veneer caliper and a sensitive electronic scale balance respectively. Information on the economics of production was gathered from market women and harvesters through the use of structured questionnaires. The present study indentified possible phenotypic differences among periwinkle harvested in Bayelsa and Rivers States base on shell length and meat weight differences. Periwinkle harvested in Rivers is bigger and heavier than those harvested in Bayelsa State. Prediction of meat and total weight of from shell length was done using regression analysis. The study also identified the involvement of the different age groups that form the work-force in the States; showing that there are more young adults and teenagers in the business of harvesting and sales than aged people. There are more men involved in the business than women. There is a decline in size, quantity and availability of periwinkle presently than in the past, a factor that could be attributed to over-harvesting.Key Words: Bayelsa, Morphology, Niger Delta, Periwinkle, Rivers

Multi-Strain Probiotics: Synergy among Isolates Enhances Biological Activities

The use of probiotics for health benefits is becoming popular because of the quest for safer products with protective and therapeutic effects against diseases and infectious agents. The emergence and spread of antimicrobial resistance among pathogens had prompted restrictions over the non-therapeutic use of antibiotics for prophylaxis and growth promotion, especially in animal husbandry. While single-strain probiotics are beneficial to health, multi-strain probiotics might be more helpful because of synergy and additive effects among the individual isolates. This article documents the mechanisms by which multi-strain probiotics exert their effects in managing infectious and non-infectious diseases, inhibiting antibiotic-resistant pathogens and health improvement. The administration of multi-strain probiotics was revealed to effectively alleviate bowel tract conditions, such as irritable bowel syndrome, inhibition of pathogens and modulation of the immune system and gut microbiota. Finally, while most of the current research focuses on comparing the effects of multi-strain and single-strain probiotics, there is a dearth of information on the molecular mechanisms of synergy among multi-strain probiotics isolates. This forms a basis for future research in the development of multi-strain probiotics for enhanced health benefits

A Comparative Study of Genetic Diversity and Multiplicity of Infection in Uncomplicated <i>Plasmodium falciparum</i> Infections in Selected Regions of Pre-Elimination and High Transmission Settings Using <i>MSP1</i> and <i>MSP2</i> Genes

Background: Understanding the genetic structure of P. falciparum population in different regions is pivotal to malaria elimination. Genetic diversity and the multiplicity of infection are indicators used for measuring malaria endemicity across different transmission settings. Therefore, this study characterized P. falciparum infections from selected areas constituting pre-elimination and high transmission settings in South Africa and Nigeria, respectively. Methods: Parasite genomic DNA was extracted from 129 participants with uncomplicated P. falciparum infections. Isolates were collected from 78 participants in South Africa (southern Africa) and 51 in Nigeria (western Africa). Allelic typing of the msp1 and msp2 genes was carried out using nested PCR. Results: In msp1, the K1 allele (39.7%) was the most common allele among the South African isolates, while the RO33 allele (90.2%) was the most common allele among the Nigerian isolates. In the msp2 gene, FC27 and IC3D7 showed almost the same percentage distribution (44.9% and 43.6%) in the South African isolates, whereas FC27 had the highest percentage distribution (60.8%) in the Nigerian isolates. The msp2 gene showed highly distinctive genotypes, indicating high genetic diversity in the South African isolates, whereas msp1 showed high genetic diversity in the Nigerian isolates. The RO33 allelic family displayed an inverse relationship with participants’ age in the Nigerian isolates. The overall multiplicity of infection (MOI) was significantly higher in Nigeria (2.87) than in South Africa (2.44) (p P. falciparum that were observed in this study could provide surveillance data, on the basis of which appropriate control strategies should be adopted

Design of a Multiepitope Vaccine against Chicken Anemia Virus Disease

Chicken anemia virus (CAV) causes severe clinical and sub-clinical infection in poultry globally and thus leads to economic losses. The drawbacks of the commercially available vaccines against CAV disease signal the need for a novel, safe, and effective vaccine design. In this study, a multiepitope vaccine (MEV) consisting of T-cell and B-cell epitopes from CAV viral proteins (VP1 and VP2) was computationally constructed with the help of linkers and adjuvant. The 3D model of the MEV construct was refined and validated by different online bioinformatics tools. Molecular docking showed stable interaction of the MEV construct with TLR3, and this was confirmed by Molecular Dynamics Simulation. Codon optimization and in silico cloning of the vaccine in pET-28a (+) vector also showed its potential expression in the E. coli K12 system. The immune simulation also indicated the ability of this vaccine to induce an effective immune response against this virus. Although the vaccine in this study was computationally constructed and still requires further in vivo study to confirm its effectiveness, this study marks a very important step towards designing a potential vaccine against CAV disease

Screening of apical membrane antigen-1 (AMA1), dense granule protein-7 (GRA7) and rhoptry protein-16 (ROP16) antigens for a potential vaccine candidate against Toxoplasma gondii for chickens

DATA AVAILABILITY : Data will be made available on request.Toxoplasmosis is a zoonotic disease caused by the protozoan parasite, Toxoplasma gondii known to infect almost all animals, including birds and humans globally. This disease has impacted the livestock industry and public health, where infection of domestic animals increases the zoonotic risk of transmission of infection to humans, threatening public health. Hence the need to discover novel and safe vaccines to fight against toxoplasmosis. In the current study, a novel multiepitope vaccine was designed using immunoinformatics techniques targeting T. gondii AMA1, GRA7 and ROP16 antigens, consisting of antigenic, immunogenic, non-allergenic and cytokine inducing T-cell (9 CD8+ and 15 CD4+) epitopes and four (4) B-cell epitopes fused together using AAY, KK and GPGPG linkers. The tertiary model of the proposed vaccine was predicted and validated to confirm the structural quality of the vaccine. The designed vaccine was highly antigenic (antigenicity = 0.6645), immunogenic (score = 2.89998), with molecular weight of 73.35 kDa, instability and aliphatic index of 28.70 and 64.10, respectively; and GRAVY of 0.363. The binding interaction, stability and flexibility were assessed with molecular docking and dynamics simulation, which revealed the proposed vaccine to have good structural interaction (binding affinity = 106.882 kcal/mol) and stability when docked with Toll like receptor-4 (TLR4). The results revealed that the Profilin-adjuvanted vaccine is promising, as it predicted induction of enhanced immune responses through the production of cytokines and antibodies critical in blocking host invasion.The National Research Foundation (NRF) of South Africa.https://www.elsevier.com/locate/jvacxam2023Veterinary Tropical DiseasesSDG-03:Good heatlh and well-bein