Protein interaction mapping related to to becker muscular dystrophy

Objective Becker Muscular Dystrophy (BMD) is a neuromuscular disorder which is incurable. In this research protein interaction network of most associated proteins with BMD to provide better clarification of disorder underlying mechanism was investigated. Materials & Methods The related genes to BMD were retrieved via string database and conducted by Cytoscape and the related algorithms. The network centrality analysis was performed based on degree, betweenness, closeness, and stress parameters. Gene ontology and clustering were performed via ClueGO analysis. Results DMD as the super-hub as well as other central proteins including UTRN, TTN, DNM2, and RYR1 are important in BMD in terms of interactive features. The impairment of muscular contraction may be vital in BMD disease pathogenesis as it is the highlighted biological process term obtained by ClueGO analysis. Conclusion DMD targeting may be the main concern for dystrophy clinical approaches. However, the other suggested proteins should be evaluated. Targeting these key proteins are required for treatment goals following extensive validation studies. © 2019, Iranian Child Neurology Society. All rights reserved

Protein-protein interaction network analysis of human fibroblast cells treated with ethanol

Introduction: Studies show that ethanol can induce changes in proteomic profile of human fibroblast cells. Some of these proteins are important in promoting cancer. Thus, analyzing function and interaction networks of these proteins are essential for better understanding the carcinogenesis mechanism of ethanol. Materials and Methods: In this study the protein-protein interaction network (PPI) of six significant down-regulated proteins in human fibroblast cells (HFFF2) treated with ethanol were analyzed by using Cytoscape software and its algorithms. Results: PPI network analysis showed that the constructed network consisted of 756 nodes and 1166 edges. Results indicated that Heterogeneous nuclear ribonucleoprotein A1 with degree = 528 and Betweenness Centrality = 0.74 is a hub protein that ethanol can alter its expression. In addition, module evaluation showed that the hub protein has a key role in different overlapped complexes. On the other hand, annotation studies by using DAVID program indicated that this protein is involved in different important biological processes in the cell. Conclusion: The six down-regulated proteins treated with ethanol may become carcinogenic and can impose vast alterations in other vital biological processes of the cell. Among them, Heterogeneous nuclear ribonucleoprotein A1 is the most important one. © 2016, Semnan University of Medical Sciences. All rights reserved

The Prevalence of Musculoskeletal Injuries in Junior Elite Squash Players

Background: The dynamics and competitiveness of squash place a high demand on the musculoskeletal system of players, thus increasing the injury risk of youth squash players. Objectives: The purpose of this study was to analyze and report the musculoskeletal injury epidemiology of junior elite squash players. Methods: Twenty-one male adolescent squash players were included in this retrospective analysis of routinely collected data over a period of 6-years. Origin, location, diagnosis and incidence of all injuries are reported. Results: A total of 212 injuries were recorded: 58% of these injuries (n = 124) were squash (on-court) related, 16% (n = 33) were because of other training activities and 26% of these injuries were (n = 55) non-squash related. Most of squash related training injuries were in the lower limbs (67%, n = 105). The most frequently reported diagnosis was “osteochondrosis” (22%, n = 34), followed by “contusion” (13%, n = 20). Time-loss injuries accounted for 103 injuries and the overall injury incidence was 4.78 per 1000 hours of training. Conclusions: It was established that pre-mature squash players are at greater risk of growth related injuries during their peak growth period. A significant number of injuries at junior level are found to occur outside of training time and result in periods of time loss. As a result, skill acquisition is negatively affected which in turn negatively affects development opportunities in junior athletes

Physical therapy in early management of the burn patient.

Profonde ou atteignant une large surface cutanée, la brûlure engendre souvent des séquelles fonctionnelles impor- tantes. Ces séquelles se constituent précocement et ont des répercussions non négligeables sur la qualité de vie des patients. Pour les prévenir et les traiter, le kinésithérapeute est un acteur important dans leur prise en charge précoce. Il devra évaluer les fonctions respiratoires, musculaires, orthopédiques mais également cutanées. L’objectif est de définir des stratégies de traitement faisant appel à des techniques de kinésithérapie classiques adaptées à cette pathologie spécifique