Linear polarization Yb³⁺-doped fiber laser with novel innerclad structures

Results on high radiance Yb3+-doped fiber lasers with novel double innerclad structures (double-D clad and four hole) and polarized output at ≈1090 nm are presented. We have demonstrated >40% of the total output power being polarized, making the fiber laser suitable for LIDAR and second-harmonic generation (SHG) applications. It also showed a 10-nm tuning range with low (less than 10 mW) average power variations. The narrow linewidth source was pumped with a low cost, low brightness laser diode, and exhibited a relatively low slope efficiency, which gives room for improvement by using a 976-nm pump source where Yb3+ has a narrower linewidth and at least five times higher absorption

Thermal Diffusion and Specular Reflection, Monte Carlo-based Study on Human Skin via Pulsed Fiber Laser Energy

The aim of traditional Chinese medicine (TCM) in acupuncture is sometimes to restore and regulate energy balance by stimulating specific points along the specific meridians traced on the human body via different techniques such as mechanical pressure, moxibustion and others. Hence, physicians have struggled to improve treatment for common diseases such as migraine and headaches. Heat stimulation and some pharmacological effects from moxa have been attributed to the therapeutic efficacy of such techniques. As heat can diffuse through the tissue, skins temperature will rise in the surrounding tissue. In this work, heat diffusion on a simple, 5-layer model of human skin is presented. Based on this, and by using Monte Carlo techniques, a photon or a photon package is launched into the tissue for mimicking the propagation of such photons at two different wavelengths through the tissue. The method generally describes the scholastic nature of radiation interactions. Most of the laser energy is deposited within a volume which cross-sectional area is the size of the beam itself. As could be seen, in the epidermis layer of the model, the heat does not go deep and nearly all the heat diffusion occurs on the edges of the beam, causing losses. Heat dissipation occurs faster and goes down to 2°C in the adipose tissue since there is low water content in this region. On the contrary, there is a fast heat increase in the muscle layer, up to 6°C at the most superficial layer. Since melanin is the most important epidermal chromophore, it can be noted that light shows strong absorption via melanin, at 690nm laser wavelength. In the papillary dermis the heat decreases and spreads out to the surrounding tissue. Once it reaches the adipose tissue, the heat is not absorbed enough; therefore, it is transmitted into the muscle, where the temperature rise is higher and reaches nearly 40 °C. Finally, photodynamics in a simple 5-layer skin model were explored at two laser wavelengths: 690nm and 1069nm, where no thermal damage would be expected, given the energy level of the employed pulses. Such pulsed laser energy levels remain to be tested in living tissue

High heart rate associated early repolarization causes J‐waves in both zebra finch and mouse

Animal science

Loss of bone morphogenetic protein signaling in fibroblasts results in CXCL12-driven serrated polyp development

Mutations in Bone Morphogenetic Protein (BMP) Receptor (BMPR)1A and SMAD4 are detected in 50% of juvenile polyposis syndrome (JPS) patients, who develop stroma-rich hamartomatous polyps. The established role of stromal cells in regulating BMP activity in the intestine implies a role for stromal cells in polyp development. We used conditional Cre-LoxP mice to investigate how specific loss of BMPR1A in endothelial cells, fibroblasts, or myofibroblasts/smooth muscle cells affects intestinal homeostasis. Selective loss of BMPR1A in fibroblasts causes severe histological changes in the intestines with a significant increase in stromal cell content and epithelial cell hyperproliferation, leading to numerous serrated polyps. This phenotype suggests that crucial changes occur in the fibroblast secretome that influences polyp development. Analyses of publicly available RNA expression databases identified CXCL12 as a potential candidate. RNAscope in situ hybridization showed an evident increase of Cxcl12-expressing fibroblasts. In vitro, stimulation of fibroblasts with BMPs resulted in downregulation of CXCL12, while inhibition of the BMP pathway resulted in gradual upregulation of CXCL12 over time. Moreover, neutralization of CXCL12 in vivo in the fibroblast-specific BMPR1A KO mice resulted in a significant decrease in polyp formation. Finally, in CRC patient specimens, mRNA-expression data showed that patients with high GREMLIN1 and CXCL12 expression had a significantly poorer overall survival. Significantly higher GREMLIN1, NOGGIN, and CXCL12 expression were detected in the Consensus Molecular Subtype 4 (CMS4) colorectal cancers, which are thought to arise from serrated polyps. Taken together, these data imply that fibroblast-specific BMP signaling-CXCL12 interaction could have a role in the etiology of serrated polyp formation.Cellular mechanisms in basic and clinical gastroenterology and hepatolog

Barrett esophagus and cancer: pathogenesis, carcinogenesis, and diagnostic dilemmas

A metaplastic process, in which native squamous epithelium of the distal esophagus is replaced by columnar epithelium, is known as Barrett esophagus (BE). Over the past years, intestinal metaplasia was recognized as a marker for BE. The risk for the development of esophageal adenocarcinoma in a patients with BE is much hi"gh er when com~aredto the normal population. Duodeno-gastro-esophageal reflux is supposed to play a role in the pathogenesis of BE and rising incidence of adenocarcinoma of the esophagus. With current therapeutic options, when clinical manifestation of this cancer occurs, it is too late for cure in the majority of patients. Therefore, attention should be focused on early diagnosis, for which molecular genetic techniques might become available. Current data on genetic alterations involved in carcinogenesis of BE are discussed. Grading of dysplasia in BE carries important clinical consequences for the individual patient: intensification of endoscopic surveillance or 'prophylactic esophagectomy'. Several morpho- andlor cytometric parameters may be used for discrimination between different grades of dysplasia in BE. Therefore, a new and original algorythm for the potential application of quantitative pathology in grading of dysplasia in patients with BE has been proposed. Molecular biology together with image analysis of histological spectrum of BE enable better understanding of the mechanisms of malignant degeneration and might ultimately lead to targeted cancer prevention andlor therapeutic interventions

Clinical applications of detecting dysfunctional p53 tumor suppressor protein

The p53 gene encodes for a protein, p53, which plays a critical role in controlling the cell cycle, in DNA repair and in programed cell death (apoptosis). p53 is one of the most frequently mutated genes in human neoplasms and a variety of techniques have been developed to detect these mutations. These range from advanced molecular-genetic analyses to immunohistochemical staining for the p53 protein. This review will summarize our current understanding of the function of p53 as well as current methods to detect dysfunctional p53 and the clinical value of such analyses.

Characteristics of Q-switched cladding-pumped ytterbium-doped fiber lasers with different high-energy fiber designs

We theoretically and experimentally analyze Q-switched cladding pumped ytterbium-doped fiber lasers designed for high pulse energies. We compare the extractable energy from two high-energy fiber designs: (1) single- or few-moded low-NA large mode area (LMA) fibers and (2) large-core multimode fibers, which may incorporate a fiber taper for brightness enhancement. Our results show that the pulse energy is proportional to the effective core area and, therefore, LMA fibers and multimode fibers of comparable core size give comparable results. However, the energy storage in multimode fibers is mostly limited by strong losses due to amplified spontaneous emission (ASE) or even spurious lasing between pulses. The ASE power increases with the number of modes in a fiber. Furthermore, spurious feedback is more difficult to suppress with a higher NA, and Rayleigh back-scattering increases with higher NA, too. These effects are smaller in low-NA LMA fibers, allowing for somewhat higher energy storage. For the LMA fibers, we found that facet damage was a more severe restriction than ASE losses or spurious lasing. With a modified laser cavity, we could avoid facet damage in the LMA fiber, and reached output pulse energies as high as 2.3 mJ, limited by ASE. Theoretical estimates suggest that output pulse energies around 10 mJ are feasible with a larger core fiber, while maintaining a good beam qualit

Wide wavelength-tuning of a double-clad Yb3+-doped fiber laser based on a fiber Bragg grating array

We report wide wavelength tuning in a double-clad ytterbium-doped fiber laser. The laser cavity consists of an array of broadband high-reflection fiber Bragg gratings and a bulk grating as output coupler and wavelength selection element. The proposed fiber laser configuration combines low intra-cavity loss of fiber Bragg grating mirrors with wide wavelength tuning of bulk gratings.We demonstrate >70 nm wavelength tuning range, limited only by the available fiber Bragg gratings