Evaluación de hongos endófitos de frutilla como antagonistas de neopestalotiopsis clavispora, patógeno de raíz y corona

PosterLos hongos de raíz-corona ocasionan grandes pérdidas en el cultivo de frutilla (Fragariaxananassa Duch.). Entre éstos, Neopestalotiopsis clavispora es un patógeno que provoca grandes pérdidas debido a la muerte de las plantas afectadas. En la actualidad es cada vez más requerido el uso de insumos biológicos para el manejo de patógenos de cultivos. Con este fin, se evaluó el efecto antagonista de hongos en dófitos aislados a partir de plantas de frutillas obre N. clavispora.Instituto de Patología VegetalFil: Meneguzzi, Natalia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Obregon, Veronica Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bella Vista; ArgentinaFil: Valetti, Lucio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Bianco, María Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales ; Argentin

Biofumigation experiences in Argentina

Artículo publicado en el sitio de Harper Adams University. Centre for Integrated Pest Management. International Biofumigation Network recopila información del uso de la biofumigación en cultivos hortícolas en distintas regiones de nuestro país.EEA San PedroFil: Mitidieri, Mariel Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; ArgentinaFil: Peralta, Romina. Universidad Nacional de Rosario; ArgentinaFil: Barbieri, Martín Osvaldo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; ArgentinaFil: Brambilla, María Virginia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; ArgentinaFil: Piris, Estela Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; ArgentinaFil: Sasia, Fabiana. Profesional actividad privada; ArgentinaFil: Obregón, Verónica Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bella Vista; ArgentinaFil: Vasquez, Pablo Antonio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Alto Valle; ArgentinaFil: Reybet, Graciela. Universidad Nacional del Comahue; ArgentinaFil: Baron, Claudio. Corporación del Mercado Central de Buenos Aires; Argentina

Biofumigation Experiences in Argentina : Short Report

Biofumigation experiences in Argentina have been held along a wide territory, and have proved to be much more effective when combined with solarization. These practices have been successfully implemented, allowing the disinfection of soils in a sustainable manner and the improvement of their physical, chemical and biological properties. In Corrientes a subtropical province specialized in off season production, incorporation of chicken and cattle manure into the greenhouse soil prior to solarization was effective against Ralstonia solanacearum, Pythium aphanidermatum, Rhizoctonia solani and Sclerotium rolfsii, other biofumigants essayed were pine tree fallen leaves, grass, cabbage and sorghum. In the centre of Argentina, horticultural and ornamental crops are grown under mild winter climate. Biosolarization (biofumigation + solarization) was effective controlling Pyrenochaeta lycopersici, Fusarium solani, Sclerotium rolfsii and Sclerotinia sclero tiorum, weeds and damping off pathogens, as well as nematodes like Nacobbus aberrans, Helycotylenchus and Criconemella. The amendments used were chicken manure, broccoli, sorghum, tomato and pepper crop debris, mustard, rapeseed and Brassica campestris. At the west of the country, in Mendoza a province with arid and continental weather, summer is hot, and good control of strawberry diseases as Phytophthora, Rhizoctonia, Phytium, Verticilium, Macrophomina, and nematodes as Meloidogyne, Ditylenchus has been achieved using rapeseed as fumigant in the greenhouse. In Bahía Blanca, a city at the south of Buenos Aires province with a colder weather Meloidogyne hapla was controlled using cattle manure and cauliflower in spring and summer in the greenhouse, nematode s of the same genus were controlled in winter using Melia azedarach seeds as fumigant. At the North of Patagonia, a semiarid region with hot summers but very cold winters, weeds in onion open field nurseries were controlled in summer using chicken manure and cabbage. Similar results were obtained at the northwest of Rio Negro province, were weeds were controlled using cabbage in spring for open field tomato crops. In the same province Fusarium oxysporum in onion was controlled using cabbage in autumn and summer.EEA San PedroFil: Mitidieri, Mariel Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; ArgentinaFil: Peralta, Romina. Universidad Nacional de Rosario; ArgentinaFil: Barbieri, Martín Osvaldo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; ArgentinaFil: Brambilla, María Virginia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; ArgentinaFil: Piris, Estela Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; ArgentinaFil: Obregón, Verónica Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bella Vista; ArgentinaFil: Vásquez, Pablo Antonio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Alto Valle; ArgentinaFil: Iriarte, Liliana. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaFil: Reybet, Graciela. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; ArgentinaFil: Barón, Claudio. Universidad de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Cuellas, Marisol Virginia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Área Metropolitana de Buenos Aires. Agencia de Extensión Rural La Plata; ArgentinaFil: Garbi, Mariana. Universidad Nacional de Luján. Departamento de Tecnología; ArgentinaFil: Martínez, Susana. Universidad Nacional del Noroeste de la Provincia de Buenos Aires; ArgentinaFil: Amoia, Rita Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Área Metropolitana de Buenos Aires. Agencia de Extensión Rural La Plata; ArgentinaFil: Delmazzo, Pablo Ricardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Área Metropolitana de Buenos Aires. Agencia de Extensión Rural La Plata; ArgentinaFil: Sordo, María Del Huerto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Agencia de Extensión Rural Monte Vera; ArgentinaFil: Adlercreutz, Enrique. INTA, Estación Experimental Agropecuaria Balcarce, Agencia de Extensión Mar del Plata, ArgentinaFil: Puerta, Analia Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; ArgentinaFil: Puerta, Analia Veronica. Universidad Nacional de Luján. Departemento de Tecnología; Argentin

Oomycetes associated with strawberry diseases in Corrientes, Argentina

Oomycetes species are widely distributed and represent a significant threat to horticulture, forestry and agriculture. Several factors, such as initial health condition of the plants, date of transplant, environmental conditions and crop management practices contribute to the incidence of these pathogens, leading to important economic losses. Despite the importance of the diseases caused by Oomycetes in vegetable crops in Corrientes (Argentina), there is a serious lack of information about host-pathogen interaction, which makes it difficult to establish efficient control strategies. The objective of this work was to characterize and identify pathogenic oomycetes of strawberry crown and roots in Corrientes. A total of 7 isolates were obtained after field sampling. Morphological and molecular characterization was carried out as well as pathogenicity tests. Five different species of oomycetes were identified: Pythium irregulare, Pythium spinosum, Phytophthora cactorum, Phytopythium vexans and Phytopythium mercuriale. To our knowledge, this is the first record of Pythium irregulare, Phytopythium mercuriale and P. vexans in strawberry in Argentina.EEA Bella VistaFil: Ibañez, Julia Magalí. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bella Vista; Argentina.Fil: Favaro, María Alejandra. Universidad Nacional del Litoral. Instituto de Ciencias Agropecuarias del Litoral; ArgentinaFil: Obregón, Verónica Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bella Vista; Argentina.Fil: Lattar, Tatiana Elisabet. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bella Vista; Argentina.Fil: Favaro, María Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Agropecuarias del Litoral; Argentin

Homozygous N540K hypochondroplasia-First report: Radiological and clinical features

We describe a 16-month-old male with N540K homozygous mutation in the FGFR3 gene who showed a more severe phenotype than hypochondroplasia (HCH). To our knowledge, a homozygous state for this mutation causing HCH has not been reported before. The clinical and radiological characteristics of our patient represent an intermediate condition between achondroplasia and achondroplasia/hypochondroplasia compound heterozygosity. This case represents a new expression of FGFR3 spectrum and it is of considerable importance for the genetic counseling in cases where both parents are affected with HCH.Fil: García de Rosa, María Laura. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría ; ArgentinaFil: Fano, Virginia. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría ; ArgentinaFil: Araoz, H. Verónica. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría ; ArgentinaFil: Chertkoff, Lilien Patricia. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría ; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Obregon, M. Gabriela. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría ; Argentin

Correção cirúrgica da origem anômala de artéria pulmonar direita em aorta ascendente

A origem anômala da artéria pulmonar direita em aorta ascendente é uma malformação congênita rara. Descrevemos o caso de uma criança submetida à correção cirúrgica com anastomose direta entre a artéria pulmonar direita e o tronco pulmonar. Após 18 meses a paciente permanece assintomática sem apresentar estenose residual significativa na angioressonância

Clinical and molecular characterization of mitochondrial DNA disorders in a group of Argentinian pediatric patients

Objective: To describe the clinical and molecular features of a group of Argentinian pediatric patients with mitochondrial DNA (mtDNA) disorders, and to evaluate the results of the implementation of a classical approach for the molecular diagnosis of mitochondrial diseases. Methods: Clinical data from 27 patients with confirmed mtDNA pathogenic variants were obtained from a database of 89 patients with suspected mitochondrial disease, registered from 2014 to 2020. Clinical data, biochemical analysis, neuroimaging findings, muscle biopsy and molecular studies were analyzed. Results: Patients were 18 females and 9 males, with ages at onset ranging from 1 week to 14 years (median = 4 years). The clinical phenotypes were: mitochondrial myopathy, encephalopathy, lactic acidosis, and stroke-like episodes (MELAS) syndrome (n = 11), Leigh syndrome (n = 5), Kearns-Sayre syndrome (n = 3), Chronic Progressive External Ophthalmoplegia (n = 2), Leber hereditary optic neuropathy (n = 2), myoclonic epilepsy associated with ragged-red fibers (n = 1) and reversible infantile myopathy with cytochrome-C oxidase deficiency (n = 3). Most of the patients harbored pathogenic single nucleotide variants, mainly involving mt-tRNA genes, such as MT-TL1, MT-TE and MT-TK. Other point variants were found in complex I subunits, like MT-ND6, MT-ND4, MT-ND5; or in MT-ATP6. The m.13513G > A variant in MT-ND5 and the m.9185 T > C variant in MT-ATP6 were apparently de novo. The rest of the patients presented large scale-rearrangements, either the ?common? deletion or a larger deletion. Conclusions: This study highlights the clinical and genetic heterogeneity of pediatric mtDNA disorders. All the cases presented with classical phenotypes, being MELAS the most frequent. Applying classical molecular methods, it was possible to achieve a genetic diagnosis in 30% of the cases, suggesting that this is an effective first approach, especially for those centers from low-middle income countries, leaving NGS studies for those patients with inconclusive results.Fil: Loos, Mariana Amina. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; ArgentinaFil: Gomez, Gimena. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; ArgentinaFil: Mayorga, Lía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Caraballo, Roberto Horacio. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Eiroa, Hernán Diego. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; ArgentinaFil: Obregon, María Gabriela. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; ArgentinaFil: Rugilo, Carlos. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; ArgentinaFil: Lubieniecki, Fabiana. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; ArgentinaFil: Taratuto, Ana Lía. No especifíca;Fil: Saccoliti, María. No especifíca;Fil: Alonso, Cristina Noemí. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; ArgentinaFil: Aráoz, Hilda Verónica. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; Argentin

Moonlighting Peptides with Emerging Function

<div><p>Hunter-killer peptides combine two activities in a single polypeptide that work in an independent fashion like many other multi-functional, multi-domain proteins. We hypothesize that emergent functions may result from the combination of two or more activities in a single protein domain and that could be a mechanism selected in nature to form moonlighting proteins. We designed moonlighting peptides using the two mechanisms proposed to be involved in the evolution of such molecules (<em>i.e</em>., to mutate non-functional residues and the use of natively unfolded peptides). We observed that our moonlighting peptides exhibited two activities that together rendered a new function that induces cell death in yeast. Thus, we propose that moonlighting in proteins promotes emergent properties providing a further level of complexity in living organisms so far unappreciated.</p> </div

Antibacterial and mitochondrial swelling activities of Iztli peptides.

<p>The activity for Iztli peptide IP1 (20 µM) (▪ in <b>A</b>, black bars in <b>B</b>) against the bacteria <i>E. coli</i> (DH10B) was tested in two ways: following the optical cell density of the culture at 600 nm (<b>A</b>) and counting the colony forming units (CFU) (<b>B</b>). Three controls are included: LB with no peptide (<b>▪</b> in <b>A</b>, white bars in <b>B</b>)<b>,</b> α-pheromone (25 µM; ▪ in <b>A</b>, gray bars in <b>B</b>) and the six residues at the N-terminus of IP1 (fIP1) (119 µM; ⋄ in <b>A</b>)<b>.</b> The results of 4 experiments are presented for plots in <b>A</b> and <b>B</b>. The bars represent standard deviations. Mitochondrial swelling was followed at 540 nm (<b>C</b>) in the presence of Iztli peptide IP1 (▪ 117 µM); three controls were used: distilled water (<b>▪)</b>, α-pheromone (▪ 160 µM) and fIP1 (⋄ 100 µM), Polyethylene glycol 3.4 kDa was added at 180 seconds. Only the IP1 data is shown here; the 3 other IP peptides display similar activity than IP1.</p