Draft genome sequence of Pantoea ananatis strain AMG521, a rice plant growth-promoting bacterial endophyte isolated from the Guadalquivir Marshes in Southern Spain.

The rice endophyte Pantoea ananatis AMG521 shows several plant growth-promoting properties and promotes rice yield increases.Its draft genome was estimated at 4,891,568 bp with 4,704 coding sequences (CDS). The genome encodes genes for N-acylhomoserine lactone (AHL) synthases, AHL hydrolases, hyperadherence (yidQ, yidP, and yidR), fusaric acid resistance, andoxidation of lignin, highlighting its biotechnological potential

Genome sequence of Pantoea sp. strain 1.19, isolated from rice rhizosphere, with the capacity to promote growth of legumes and nonlegumes.

Made available in DSpace on 2017-11-22T23:20:51Z (GMT). No. of bitstreams: 1 2017MegiasetalGenomeAnnouncPantoea1.19.pdf: 126531 bytes, checksum: 7cfdbca5f1de71fa233ed687cebf351f (MD5) Previous issue date: 2017-11-22bitstream/item/167212/1/2017MegiasetalGenomeAnnounc-Pantoea1.19.pd

RNA-seq analysis of the Rhizobium tropici CIAT 899 Transcripitome shows similarites in the activation patterns of symbiotic genes in the presence of apigenin and salt.

Rhizobium tropici strain CIAT 899 establishes effective symbioses with several legume species, including Phaseolus vulgaris and Leucaena leucocephala. This bacterium synthesizes a large variety of nodulation factors in response to nod-gene inducing flavonoids and, surprisingly, also under salt stress conditions. The aim of this study was to identify differentially expressed genes in the presence of both inducer molecules, and analyze the promoter regions located upstream of these genes. Results obtained by RNA-seq analyses of CIAT 899 induced with apigenin, a nod gene-inducing flavonoid for this strain, or salt allowed the identification of 19 and 790 differentially expressed genes, respectively. Fifteen of these genes were up-regulated in both conditions and were involved in the synthesis of both Nod factors and indole-3-acetic acid. Transcription of these genes was presumably activated through binding of at least one of the five NodD proteins present in this strain to specific nod box promoter sequences when the bacterium was induced by both apigenin and salt. Finally, under saline conditions, many other transcriptional responses were detected, including an increase in the transcription of genes involved in trehalose catabolism, chemotaxis and protein secretion, as well as ribosomal genes, and a decrease in the transcription of genes involved in transmembrane transport. To our knowledge this is the first time that a transcriptomic study shows that salt stress induces the expression of nodulation genes in the absence of flavonoids. Thus, in the presence of both nodulation inducer molecules, apigenin and salt, R. tropici CIAT 899 up-regulated the same set of symbiotic genes. It could be possible that the increases in the transcription levels of several genes related to nodulation under saline conditions could represent a strategy to establish symbiosis under abiotic stressing conditions

Analysis of Forces Involved in the Perching Maneuver of Flapping-Wing Aerial Systems and Development of an Ultra-Lightweight Perching System

Trying to optimize the design of aerial robotics systems, this work presents an optimized low-weight landing system for flapping-wing aerial robots. The design, based on the use of low-sized neodymium magnets, intends to provide that these aerial robots have the capability of landing in restricted areas by using the presented solution. This capacity will increase the application range of these robots. A study of this situation has been done to analyze the perching maneuver forces and evaluate the system. The solution presented is low-weight, lowsized, and also relatively inexpensive. Therefore, this solution may apply to most ornithopter robots. Design, analysis of the implied forces, development and experimental validation of the idea are presented in this work, demonstrating that the developed solution can overcome the ornithopter’s payload limitation providing an efficient and reliable solutionUnión Europea SI-1867/23/2018 ERC-AD

Uso de metabolitos microbianos como aditivos en inoculantes.

Título do evento por extenso: REUNIÃO DA REDE DE LABORATÓRIOS PARA RECOMENDAÇÃO, PADRONIZAÇÃO E DIFUSÃO DE TECNOLOGIAS DE INOCULANTES MICROBIANOS DE INTERESSE AGRÍCOLA, 19., 2018, Foz do Iguaçu. Editores Técnicos: Jerri Édson Zilli, Fábio Bueno dos Reis Júnior

La fusión glaciar en el Pirineo: condicionantes ambientales y primeros resultados bianuales en el glaciar de Infiernos (Pirineo aragonés)

Data are presented about the environmental characters of the Infiernos glacier and of the regression suffered in the last century, as well as the meltwater flow values corresponding to two daily cycle of the Infierno glacier. These values were measured at the end of the summer of 1998 and 1999. There is a close relationship between these data and both thermic rythms. This fact suggests that meltwater flow should become a useful additional parameter to explain the environmental control of the drainage systems derived from the glacier fusion, as well as the serious glacier regression happened in the last decades, indicative of balances between the accumulation nival and the ablation clearly negative. The data are accompanied of measures of regression glacier among the summers of 1998 and 1999.Se presentan datos acerca de las características ambientales del Glaciar del Infierno y de la regresión sufrida en el Último siglo, así como los valores de los caudales de fusión de dos ciclos diarios en este glaciar, medidos a finales de dos veranos consecutivos (años 1998 y 1999). La relación entre este dato y los ritmos térmicos es muy estrecha, lo cual permite disponer de un dato más, de gran importancia, para explicar el control ambiental en los sistemas de drenaje de la fusión glaciar, así como la acusada regresión glaciar de las últimas décadas, indicativa de balances entre la acumulación nival y la ablación claramente negativos. Los datos se acompañan de medidas de retroceso glaciar entre los veranos de 1998 y 1999

Regulatory nodD1 and nodD2 genes of Rhizobium tropici strain CIAT 899 and their roles in the early stages of molecular signaling and host-legume nodulation.

Nodulation and symbiotic nitrogen fixation are mediated by several genes, both of the host legume and of the bacterium. The rhizobial regulatory nodD gene plays a critical role, orchestrating the transcription of the other nodulation genes. Rhizobium tropici strain CIAT 899 is an effective symbiont of several legumes?with an emphasis on common bean (Phaseolus vulgaris)?and is unusual in carrying multiple copies of nodD, the roles of which remain to be elucidated. Results: Phenotypes, Nod factors and gene expression of nodD1 and nodD2 mutants of CIAT 899 were compared with those of the wild type strain, both in the presence and in the absence of the nod-gene-inducing molecules apigenin and salt (NaCl). Differences between the wild type and mutants were observed in swimming motility and IAA (indole acetic acid) synthesis. In the presence of both apigenin and salt, large numbers of Nod factors were detected in CIAT 899, with fewer detected in the mutants. nodC expression was lower in both mutants; differences in nodD1 and nodD2 expression were observed between the wild type and the mutants, with variation according to the inducing molecule, and with a major role of apigenin with nodD1 and of salt with nodD2. In the nodD1 mutant, nodulation was markedly reduced in common bean and abolished in leucaena (Leucaena leucocephala) and siratro (Macroptilium atropurpureum), whereas a mutation in nodD2 reduced nodulation in common bean, but not in the other two legumes. Conclusion: Our proposed model considers that full nodulation of common bean by R. tropici requires both nodD1 and nodD2, whereas, in other legume species that might represent the original host, nodD1 plays the major role. In general, nodD2 is an activator of nod-gene transcription, but, in specific conditions, it can slightly repress nodD1. nodD1 and nodD2 play other roles beyond nodulation, such as swimming motility and IAA synthesis

Draft genome sequence of Pantoea ananatis Strain 1.38, a bacterium isolated from the rhizosphere of Oryza sativa var. puntal that shows biotechnological potential as an inoculant.

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